Journal: Iranian Journal of Biotechnology

Article Title: Targeting TPX2 to Enhance Metastasis Control and Radiosensitivity in Esophageal Squamous Cell Carcinoma Through PI3K/AKT Pathway Modulation

doi: 10.30498/ijb.2025.549444.4243

Figure Lengend Snippet: TPX2 promotes the invasion and migration ability of ESCC cells. A) Western blot analysis was conducted to assess TPX2 expression levels in ESCC cell lines (K150, K180, K410, K450, K510 and TE-1 cells). B-E) Lentivirus transfection was employed to enhance TPX2 expression in KYSE150 and KYSE510 cells. RT-qPCR and Western blotting analyses demonstrated a substantial increase in TPX2 mRNA and protein levels. Statistical analyses were performed by Student’s t-test. F) Effects of TPX2 overexpression on wound healing in K150 cells; G, H) Transwell assay was performed to analyze the effect of TPX2 on K150 cell migration and invasion in vitro . (Scale bar = 100 µm); I) Effects of TPX2 overexpression on wound healing in K510 cells; J, K) Transwell assay was performed to analyze the effect of TPX2 on K510 cell migration and invasion in vitro. (Scale bar = 100 µm) Each bar displays the mean ± SD of 3 independent experiments as analyzed by paired two-tailed Student’s t-test. * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001.

Article Snippet: For RT-qPCR gene expression analysis, SYBR Green (cat. # C0008, Target Mol) was utilized following the manufacturer’s instructions.

Techniques: Migration, Western Blot, Expressing, Transfection, Quantitative RT-PCR, Over Expression, Transwell Assay, In Vitro, Two Tailed Test

Journal: Iranian Journal of Biotechnology

Article Title: Targeting TPX2 to Enhance Metastasis Control and Radiosensitivity in Esophageal Squamous Cell Carcinoma Through PI3K/AKT Pathway Modulation

doi: 10.30498/ijb.2025.549444.4243

Figure Lengend Snippet: TPX2 affects the expression of TPX2 in surrounding cells through exosomes. A-D) Characterization of exosomes was performed using transmission electron microscopy (TEM), nanotracker (NTA). E) Western blot analysis with positive markers CD63 and TSG101. F) Labeling exosomes with the fluorescent dye PKH26 confirmed their uptake by cells. G) RT-qPCR data showed significantly higher levels of TPX2 mRNA in exosomes derived from cells overexpressing TPX2 compared to exosomes from control cells. H, I) we examined whether neighboring cells could absorb TPX2 from these exosomes. Exosomes from different sources were incubated with K150 and K510 cells. After 24 hours, cells were collected, and a Western blot analysis of TPX2 expression was conducted. *** indicates p<0.001.

Article Snippet: For RT-qPCR gene expression analysis, SYBR Green (cat. # C0008, Target Mol) was utilized following the manufacturer’s instructions.

Techniques: Expressing, Transmission Assay, Electron Microscopy, Western Blot, Labeling, Quantitative RT-PCR, Derivative Assay, Control, Incubation

Journal: bioRxiv

Article Title: Precursor of chemically expanded hepatocytes (pre-cHep) with 1-million-fold expansion potential and liver repopulation capacity

doi: 10.64898/2026.05.15.725446

Figure Lengend Snippet: Characterization of cHep. A. Direct differentiation of cryopreserved pre-cHep using a protocol equivalent to 3D PHH spheroid formation. B. Representative images of 3D PHH and cHep. C. Principal component analysis (PCA) plot including cHep generated from day 30 (D30) J03, J06, and J13 L4 pre-cHep, 3D PHH, and published hepatocyte-like cells (HLC). D. Heatmap showing expression of 264 liver-enriched genes. E–H. Distance-based similarity scores based on gene expression associated with xenobiotic metabolism (E), amino acid biosynthesis (F), bile acid biosynthesis (G), and the urea cycle (H). I. Immunofluorescence staining for albumin, HNF4α, CYP2E1, and ASGR1. J. Cholyl-lysyl-fluorescein (CLF) staining.

Article Snippet: Hierarchical clustering and gene expression heatmap analysis of 264 liver-enriched genes from Human Protein Atlas (gene list in Supplementary Table S3) also showed that D30 cHep clustered closely with 3D PHH, although expression of some genes remained lower than in uncultured PHH and liver samples ( ).

Techniques: Generated, Expressing, Gene Expression, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: Precursor of chemically expanded hepatocytes (pre-cHep) with 1-million-fold expansion potential and liver repopulation capacity

doi: 10.64898/2026.05.15.725446

Figure Lengend Snippet: Gene expression analysis of HLC. Distance-based similarity score and gene expression heatmap with KEGG gene sets: Fatty acid metabolism (A, D), Retinol metabolism (B, E) and Complement coagulation cascade (C, F).

Article Snippet: Hierarchical clustering and gene expression heatmap analysis of 264 liver-enriched genes from Human Protein Atlas (gene list in Supplementary Table S3) also showed that D30 cHep clustered closely with 3D PHH, although expression of some genes remained lower than in uncultured PHH and liver samples ( ).

Techniques: Gene Expression, Coagulation