gene exp vegfa mm00437304 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp vegfa mm00437304 m1
    DFP treatment decreases retinal markers of oxidative stress. Graph showing retinal isoprostane F2α-VI levels in the retinas of 9-month-old DKO mice treated with DFP for 5 months relative to untreated, age-matched DKO and WT mice ( A , n = 3 mice per group). DFP treatment significantly reduced Epo mRNA levels, as measured by qPCR, in the retinas of DKO mice treated for 6 months with DFP relative to untreated 9-month-old DKOs ( B , n = 3 mice per group). DFP did not change <t>Vegfa</t> mRNA levels in either neurosensory retina ( C ) or RPE/choroid ( D ), but it significantly reduced Cd68 mRNA levels in neurosensory retinas of treated DKO relative to untreated DKO controls ( E ) and showed a trend toward C3 mRNA level reduction in the RPE/choroid ( F ). *Significant difference ( P
    Gene Exp Vegfa Mm00437304 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp vegfa mm00437304 m1 - by Bioz Stars, 2021-03
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    Images

    1) Product Images from "The Oral Iron Chelator Deferiprone Protects against Iron Overload–Induced Retinal Degeneration"

    Article Title: The Oral Iron Chelator Deferiprone Protects against Iron Overload–Induced Retinal Degeneration

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.10-6207

    DFP treatment decreases retinal markers of oxidative stress. Graph showing retinal isoprostane F2α-VI levels in the retinas of 9-month-old DKO mice treated with DFP for 5 months relative to untreated, age-matched DKO and WT mice ( A , n = 3 mice per group). DFP treatment significantly reduced Epo mRNA levels, as measured by qPCR, in the retinas of DKO mice treated for 6 months with DFP relative to untreated 9-month-old DKOs ( B , n = 3 mice per group). DFP did not change Vegfa mRNA levels in either neurosensory retina ( C ) or RPE/choroid ( D ), but it significantly reduced Cd68 mRNA levels in neurosensory retinas of treated DKO relative to untreated DKO controls ( E ) and showed a trend toward C3 mRNA level reduction in the RPE/choroid ( F ). *Significant difference ( P
    Figure Legend Snippet: DFP treatment decreases retinal markers of oxidative stress. Graph showing retinal isoprostane F2α-VI levels in the retinas of 9-month-old DKO mice treated with DFP for 5 months relative to untreated, age-matched DKO and WT mice ( A , n = 3 mice per group). DFP treatment significantly reduced Epo mRNA levels, as measured by qPCR, in the retinas of DKO mice treated for 6 months with DFP relative to untreated 9-month-old DKOs ( B , n = 3 mice per group). DFP did not change Vegfa mRNA levels in either neurosensory retina ( C ) or RPE/choroid ( D ), but it significantly reduced Cd68 mRNA levels in neurosensory retinas of treated DKO relative to untreated DKO controls ( E ) and showed a trend toward C3 mRNA level reduction in the RPE/choroid ( F ). *Significant difference ( P

    Techniques Used: Mouse Assay, Real-time Polymerase Chain Reaction

    2) Product Images from "Developmental regression of hyaloid vasculature is triggered by neurons"

    Article Title: Developmental regression of hyaloid vasculature is triggered by neurons

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20151966

    Deletion of VEGF normalizes persistent hyaloid vessels in neuronal VEGFR2 knockout mice. (A) Quantitative PCR analysis for retinal tissues at P6 ( n = 4). (B) Representative immunoblots and quantification in three independent experiments. (C–O) Whole-mount staining of hyaloid vessels at P6 and quantification ( n = 4). Spontaneous endothelial apoptosis detected in control mice (arrowheads in K) is greatly reduced in Vegfr2 Δneuro mice and is recovered in Vegfr2 ; Vegfa Δneuro mice (arrowheads in M). (P) Schematic diagram depicting the transition of ocular circulatory systems. Bars: (C–G) 500 µm; (H–J) 200 µm; (K–M) 50 µm. *, P
    Figure Legend Snippet: Deletion of VEGF normalizes persistent hyaloid vessels in neuronal VEGFR2 knockout mice. (A) Quantitative PCR analysis for retinal tissues at P6 ( n = 4). (B) Representative immunoblots and quantification in three independent experiments. (C–O) Whole-mount staining of hyaloid vessels at P6 and quantification ( n = 4). Spontaneous endothelial apoptosis detected in control mice (arrowheads in K) is greatly reduced in Vegfr2 Δneuro mice and is recovered in Vegfr2 ; Vegfa Δneuro mice (arrowheads in M). (P) Schematic diagram depicting the transition of ocular circulatory systems. Bars: (C–G) 500 µm; (H–J) 200 µm; (K–M) 50 µm. *, P

    Techniques Used: Knock-Out, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot, Staining

    3) Product Images from "Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice"

    Article Title: Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice

    Journal:

    doi: 10.1096/fj.08-108548

    Inhibition of HDACs prevents hypoxia-induced increases in Vegfa expression in isolated cardiac myocytes. A , B ) Cells were pretreated with DMSO/TSA ( A ) or Nullscript/Scriptaid ( B ) for 1 h and exposed to 1% O 2 for 5 h. Vegfa expression levels were determined
    Figure Legend Snippet: Inhibition of HDACs prevents hypoxia-induced increases in Vegfa expression in isolated cardiac myocytes. A , B ) Cells were pretreated with DMSO/TSA ( A ) or Nullscript/Scriptaid ( B ) for 1 h and exposed to 1% O 2 for 5 h. Vegfa expression levels were determined

    Techniques Used: Inhibition, Expressing, Isolation

    Single-dose i.p. administration of HDACIs reduces I/R-induced Vegfa induction and vascular permeability in vivo . A ) Vegfa mRNA levels from the LV free wall of treated or control mice increase with ischemia, returning to baseline levels with single-dose
    Figure Legend Snippet: Single-dose i.p. administration of HDACIs reduces I/R-induced Vegfa induction and vascular permeability in vivo . A ) Vegfa mRNA levels from the LV free wall of treated or control mice increase with ischemia, returning to baseline levels with single-dose

    Techniques Used: Permeability, In Vivo, Mouse Assay

    HDAC4 plays a primary role in mediating the hypoxic response of Vegfa and Egln3 in isolated cardiac myocytes. A ) Cells were pretreated with Nullscript (N) or Scriptaid (S) for 1 h and exposed to 1% O 2 for 5 h. Quantitative RT-PCR analysis was performed
    Figure Legend Snippet: HDAC4 plays a primary role in mediating the hypoxic response of Vegfa and Egln3 in isolated cardiac myocytes. A ) Cells were pretreated with Nullscript (N) or Scriptaid (S) for 1 h and exposed to 1% O 2 for 5 h. Quantitative RT-PCR analysis was performed

    Techniques Used: Isolation, Quantitative RT-PCR

    4) Product Images from "Visceral Endoderm Expression of Yin-Yang1 (YY1) Is Required for VEGFA Maintenance and Yolk Sac Development"

    Article Title: Visceral Endoderm Expression of Yin-Yang1 (YY1) Is Required for VEGFA Maintenance and Yolk Sac Development

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0058828

    cKO embryos display a variety of defects in the yolk sac mesoderm. A–B, D–E) Whole mount immunofluorescence of 9.5 dpc WT (A–B) or mutant (D–E) yolk sacs (YS) using the endothelial marker PECAM (green) and the vascular smooth muscle marker (αSMA) demonstrates that the large disorganized vessels in the cKO (D) are not surrounded by αSMA (E). C, F) Section immunofluorescence of WT (C) and cKO (F) 9.5 dpc yolk sacs demonstrates loss of αSMA in the cKO. G–L) Section immunofluorescence of VEGFA (green) demonstrates relatively uniform VEGF levels in the 9.0, 9.25 and 9.5 dpc WT yolk sac (G–I) while VEGF distribution in the visceral endoderm of the mutant is progressively diminished at each stage (J–L). M) A Western blot of whole yolk sacs at the indicated stages. The ratio of VEGFA to GAPDH signal intensities for the cKO relative to each stage-matched WT control is displayed under each band. N) Cleaved Caspase-3 staining was used to assess the percentage of cell death in the yolk sacs layers of WT and cKO sections at 8.5 and 9.0 dpc . A significant increase in apoptosis was observed in the cKO mesoderm (ME) at 9.0 dpc . O) Phosphohistone-H3 (PH-3) staining was similarly used to assess proliferation and a significant decrease in proliferation was found in the cKO yolk sac mesoderm at 9.0 dpc . *** = p
    Figure Legend Snippet: cKO embryos display a variety of defects in the yolk sac mesoderm. A–B, D–E) Whole mount immunofluorescence of 9.5 dpc WT (A–B) or mutant (D–E) yolk sacs (YS) using the endothelial marker PECAM (green) and the vascular smooth muscle marker (αSMA) demonstrates that the large disorganized vessels in the cKO (D) are not surrounded by αSMA (E). C, F) Section immunofluorescence of WT (C) and cKO (F) 9.5 dpc yolk sacs demonstrates loss of αSMA in the cKO. G–L) Section immunofluorescence of VEGFA (green) demonstrates relatively uniform VEGF levels in the 9.0, 9.25 and 9.5 dpc WT yolk sac (G–I) while VEGF distribution in the visceral endoderm of the mutant is progressively diminished at each stage (J–L). M) A Western blot of whole yolk sacs at the indicated stages. The ratio of VEGFA to GAPDH signal intensities for the cKO relative to each stage-matched WT control is displayed under each band. N) Cleaved Caspase-3 staining was used to assess the percentage of cell death in the yolk sacs layers of WT and cKO sections at 8.5 and 9.0 dpc . A significant increase in apoptosis was observed in the cKO mesoderm (ME) at 9.0 dpc . O) Phosphohistone-H3 (PH-3) staining was similarly used to assess proliferation and a significant decrease in proliferation was found in the cKO yolk sac mesoderm at 9.0 dpc . *** = p

    Techniques Used: Immunofluorescence, Mutagenesis, Marker, Western Blot, Staining

    Changes in yolk sac gene expression in cKO embryos. A–E) RT-PCR and qPCR performed with cDNA prepared from whole 9.0 and 9.5 dpc cKO and WT yolk sacs. A) As expected, Yy1 is significantly downregulated in whole cKO yolk sacs. β-actin and Hprt expression are used as loading controls. B) No expression differences between WT and cKO samples are noted for VegfA using primers that recognize all (Exon 2–3) or the alternative VegfA isoforms (Exons 3–8) nor in the Vegf transcriptional regulator Hif1α . C) While many visceral endoderm-specific genes show no expression differences, expression of vHnf1 , Hnf4α and Pgc1α were all downregulated in cKO samples when compared to WT at 9.0 and 9.5 dpc . D) An examination of genes involved in lysosome biogenesis reveals no expression differences between WT and cKO yolk sacs with the exception of Enpp-2 , which is upregulated in mutant samples at both stages examined. E) qPCR reveals that Yy1 is expressed at ∼30% of WT levels in whole yolk sacs, where mesoderm derivatives maintain Yy1 . qPCR was used to confirm that VegfA expression is not significantly altered between cKO and WT and that expression of the visceral endoderm gene, Hnf4α is significantly downregulated in cKO yolk sacs. *** = p value
    Figure Legend Snippet: Changes in yolk sac gene expression in cKO embryos. A–E) RT-PCR and qPCR performed with cDNA prepared from whole 9.0 and 9.5 dpc cKO and WT yolk sacs. A) As expected, Yy1 is significantly downregulated in whole cKO yolk sacs. β-actin and Hprt expression are used as loading controls. B) No expression differences between WT and cKO samples are noted for VegfA using primers that recognize all (Exon 2–3) or the alternative VegfA isoforms (Exons 3–8) nor in the Vegf transcriptional regulator Hif1α . C) While many visceral endoderm-specific genes show no expression differences, expression of vHnf1 , Hnf4α and Pgc1α were all downregulated in cKO samples when compared to WT at 9.0 and 9.5 dpc . D) An examination of genes involved in lysosome biogenesis reveals no expression differences between WT and cKO yolk sacs with the exception of Enpp-2 , which is upregulated in mutant samples at both stages examined. E) qPCR reveals that Yy1 is expressed at ∼30% of WT levels in whole yolk sacs, where mesoderm derivatives maintain Yy1 . qPCR was used to confirm that VegfA expression is not significantly altered between cKO and WT and that expression of the visceral endoderm gene, Hnf4α is significantly downregulated in cKO yolk sacs. *** = p value

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Mutagenesis

    5) Product Images from "Mechanisms of Sex Differences in TNFR2 Mediated Cardioprotection"

    Article Title: Mechanisms of Sex Differences in TNFR2 Mediated Cardioprotection

    Journal:

    doi: 10.1161/CIRCULATIONAHA.107.756890

    Effects of TNF receptors on myocardial growth factor VEGF production after I/R. Relative quantitation of VEGFa mRNA compared to male wild type control (RT Real-time PCR) is shown in A (WT and TNFR2KO hearts) and B (WT' and TNFR1/2KO). Myocardial VEGF
    Figure Legend Snippet: Effects of TNF receptors on myocardial growth factor VEGF production after I/R. Relative quantitation of VEGFa mRNA compared to male wild type control (RT Real-time PCR) is shown in A (WT and TNFR2KO hearts) and B (WT' and TNFR1/2KO). Myocardial VEGF

    Techniques Used: Quantitation Assay, Real-time Polymerase Chain Reaction

    6) Product Images from "Mid- to late term hypoxia in the mouse alters placental morphology, glucocorticoid regulatory pathways and nutrient transporters in a sex-specific manner"

    Article Title: Mid- to late term hypoxia in the mouse alters placental morphology, glucocorticoid regulatory pathways and nutrient transporters in a sex-specific manner

    Journal: The Journal of Physiology

    doi: 10.1113/jphysiol.2014.272856

    The effects of maternal hypoxia on placental vasculogenesis factor expression and the insulin like growth factor system A–F , the effect of maternal exposure to hypoxia (filled bars, 12% oxygen) from E14.5 to E18.5 on the relative mRNA levels of Vegfa ( A ), Kdr ( B ), Flt1 ( C ), Igf2 ( D ), Igf1r ( E ) and Igf2r ( F ) in E18.5 placentas of male and female fetuses compared to controls (open bars). n = 9–12 per sex from 8 litters per treatment group. G and H , representative sections showing IGF1R protein levels and tissue distribution in control placentas ( G ) and hypoxic placentas ( H ). Insets in G and H show no antibody control staining in adjacent sections. Data are represented as means ± SEM, with data being normalised to the mean of the values from the male control samples * P
    Figure Legend Snippet: The effects of maternal hypoxia on placental vasculogenesis factor expression and the insulin like growth factor system A–F , the effect of maternal exposure to hypoxia (filled bars, 12% oxygen) from E14.5 to E18.5 on the relative mRNA levels of Vegfa ( A ), Kdr ( B ), Flt1 ( C ), Igf2 ( D ), Igf1r ( E ) and Igf2r ( F ) in E18.5 placentas of male and female fetuses compared to controls (open bars). n = 9–12 per sex from 8 litters per treatment group. G and H , representative sections showing IGF1R protein levels and tissue distribution in control placentas ( G ) and hypoxic placentas ( H ). Insets in G and H show no antibody control staining in adjacent sections. Data are represented as means ± SEM, with data being normalised to the mean of the values from the male control samples * P

    Techniques Used: Expressing, Staining

    Related Articles

    other:

    Article Title: The Oral Iron Chelator Deferiprone Protects against Iron Overload–Induced Retinal Degeneration
    Article Snippet: Probes used were transferrin receptor ( Tfrc , Mm00441941_m1), vascular endothelial growth factor A ( Vegfa , Mm00437304_m1), erythropoietin ( Epo , Mm00433126_m1), CD68 antigen ( Cd68 , Mm03047343_m1*), and complement component 3 ( C3 , Mm01232779_m1).

    Article Title: Mid- to late term hypoxia in the mouse alters placental morphology, glucocorticoid regulatory pathways and nutrient transporters in a sex-specific manner
    Article Snippet: The primer–probe sets employed were: Slc2a1 (glucose transporter 1, Mm00441473_m1), Slc2a3 (glucose transporter 3, Mm03053806_s1), Slc38a1 (sodium-coupled neutral amino acid transporter 1, Mm00506391_m1), Slc38a2 (sodium-coupled neutral amino acid transporter 2, Mm00628416_m1), Slc38a4 (sodium-coupled neutral amino acid transporter 4, Mm00459056_m1), Hif1a (Mm00468869_m1), Crh (corticotropin releasing hormone, Mm01293920_s1), Crhr1 (corticotropin releasing hormone receptor 1, Mm00432670_m1), Hsd11b2 (11 beta hydroxysteroid dehydrogenase type 2, Mm00492541_g1), Nr3c1 (nuclear receptor subfamily 3, group C, member 1, Mm00433832_m1), Nr3c2 (nuclear receptor subfamily 3, group C, member 2, Mm01241596_m1), Igf2 (insulin like growth factor 2, Mm00439565_g1), Igf1r (insulin like growth factor 1 receptor, Mm00802831_m1), Igf2r (insulin like growth factor 2 receptor, Mm00439576_m1), Vegfa (vascular endothelial growth factor A, Mm00437304_m1), Pgf (placental growth factor, Mm00435613_m1), Kdr (kinase insert domain receptor, Mm01222431_m1) and Flt1 (Fms related tyrosine kinase 1, Mm01222431_m1).

    Real-time Polymerase Chain Reaction:

    Article Title: Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice
    Article Snippet: Reverse transcription was carried out with 1 μg total RNA using the Superscript First-Strand Synthesis System for RT-PCR (Invitrogen), according to the manufacturer’s instructions. .. Ten nanograms of cDNA was subjected to real-time PCR using Taqman gene expression assays (Applied Biosystems, Foster City, CA, USA) to assess the expression level of Vegfa (Mm00437304_m1), Hdac2 (Mm01193631_m1), Hdac3 (Mm01258403_gH), Hdac4 (Mm01299566_m1), Hdac5 (Mm00515917_m1), Hdac6 (Mm00515945_m1), Hdac9 (Mm00458456_m1), Egln1 (Mm00459770_m1), Egln2 (Mm00519067_m1), and Egln3 (Mm00472200_m1). β 2-microglobulin (Mm00437762_m1) was used as an internal control. .. siRNA (Ambion, Foster City, CA, USA) directed against Hdac2 (ID: 158016), Hdac3 (ID: 158916), Hdac4 (ID: 166635), Hdac5 (ID: 158919), Hdac6 (ID: 158922), Hdac8 (custom), Hdac9 (ID: 174326), or scrambled siRNA (ID: AM4611) were transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions.

    Article Title: Visceral Endoderm Expression of Yin-Yang1 (YY1) Is Required for VEGFA Maintenance and Yolk Sac Development
    Article Snippet: .. Quantitative RT-PCR (qPCR) was performed using the following Taqman gene expression Assays: Yy1 (MM0456392_m1), VegfA (MM00437304_m1) and Hnf4α (MM00455964_m1). .. These assays were multiplexed with ActB using PerfeCTa® qPCR SuperMix, Low ROX™ (Quanta Biosciences).

    Article Title: Developmental regression of hyaloid vasculature is triggered by neurons
    Article Snippet: Quantitative RT-PCR analysis Total RNA was prepared from retinal tissues, and reverse transcription was performed using Superscript II (Invitrogen). .. Quantitative PCR assays were conducted with a real-time PCR system (ABI 7500 Fast) using PCR master mix (TaqMan Fast Universal; Applied Biosystems) and a TaqMan gene expression assay mix of vegfa (Mm00437304_ml) and vegfr2 (Mm00440099_ml). .. A mouse β-actin (Mm00607939_s1) assay mix served as an endogenous control.

    Article Title: Therapeutic Efficacy of Topical Epigallocatechin Gallate (EGCG) in Murine Dry Eye
    Article Snippet: Total RNA was isolated from the dissected corneal tissue using Trizol (Invitrogen) and RNeasy Microkit (Qiagen). .. The first strand of complementary DNA (cDNA) was synthesized with random hexamers using SuperScript III™ reverse transcriptase (Invitrogen) and quantitative real-time polymerase chain reaction was performed using Taqman Universal PCR Mastermix and FAM-MGB dye-labeled predesigned primers (Applied Biosystems) for IL-1β (Mm00434228_m1), TNF-α (Mm99999068_m1), CCL2 (Mm00439620_m1), VEGF-A (Mm00437304_ml), VEGF-C (Mm00437313_ml), VEGF-D (Mm00438965_ml), and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) (Mm99999915_g1). ..

    Article Title: Mechanisms of Sex Differences in TNFR2 Mediated Cardioprotection
    Article Snippet: .. 0.5 μg of total RNA was subjected to cDNA synthesis using cloned AMV first-strand cDNA synthesis kit (Invitrogen life technologies, Carlsbad, CA). cDNA from each sample was analyzed for 18S (assay ID# Hs99999901_s1), TNF (assay ID# Mm00443258_m1), IL-6 (assay ID# Mm00446190_m1), VEGFa (assay ID# Mm00437304_m1), SOCS3 (assay ID# Mm00545913_s1) and STAT3 (assay ID# Mm00456961_m1) by using TaqMan gene expression assay (Real-time PCR) (Applied Biosystems, Foster City, CA). ..

    Expressing:

    Article Title: Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice
    Article Snippet: Reverse transcription was carried out with 1 μg total RNA using the Superscript First-Strand Synthesis System for RT-PCR (Invitrogen), according to the manufacturer’s instructions. .. Ten nanograms of cDNA was subjected to real-time PCR using Taqman gene expression assays (Applied Biosystems, Foster City, CA, USA) to assess the expression level of Vegfa (Mm00437304_m1), Hdac2 (Mm01193631_m1), Hdac3 (Mm01258403_gH), Hdac4 (Mm01299566_m1), Hdac5 (Mm00515917_m1), Hdac6 (Mm00515945_m1), Hdac9 (Mm00458456_m1), Egln1 (Mm00459770_m1), Egln2 (Mm00519067_m1), and Egln3 (Mm00472200_m1). β 2-microglobulin (Mm00437762_m1) was used as an internal control. .. siRNA (Ambion, Foster City, CA, USA) directed against Hdac2 (ID: 158016), Hdac3 (ID: 158916), Hdac4 (ID: 166635), Hdac5 (ID: 158919), Hdac6 (ID: 158922), Hdac8 (custom), Hdac9 (ID: 174326), or scrambled siRNA (ID: AM4611) were transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions.

    Article Title: Visceral Endoderm Expression of Yin-Yang1 (YY1) Is Required for VEGFA Maintenance and Yolk Sac Development
    Article Snippet: .. Quantitative RT-PCR (qPCR) was performed using the following Taqman gene expression Assays: Yy1 (MM0456392_m1), VegfA (MM00437304_m1) and Hnf4α (MM00455964_m1). .. These assays were multiplexed with ActB using PerfeCTa® qPCR SuperMix, Low ROX™ (Quanta Biosciences).

    Article Title: Developmental regression of hyaloid vasculature is triggered by neurons
    Article Snippet: Quantitative RT-PCR analysis Total RNA was prepared from retinal tissues, and reverse transcription was performed using Superscript II (Invitrogen). .. Quantitative PCR assays were conducted with a real-time PCR system (ABI 7500 Fast) using PCR master mix (TaqMan Fast Universal; Applied Biosystems) and a TaqMan gene expression assay mix of vegfa (Mm00437304_ml) and vegfr2 (Mm00440099_ml). .. A mouse β-actin (Mm00607939_s1) assay mix served as an endogenous control.

    Article Title: Hepatic leptin receptor expression can partially compensate for IL-6Rα deficiency in DEN-induced hepatocellular carcinoma
    Article Snippet: Quantitative PCR was performed on an ABI Quantstudio Detector (Applied Biosystems). .. The following TaqMan probes (Applied Biosystems) were used for gene expression assays: Il-6rα (Mm00439653_ml), Lepr (Mm01262069_m1), Lepr (Mm01265583_m1), Timp1 (Mm00441818_m1), Socs3 (Mm00545913_s1), Srebp1 (Mm00550338_m1), Srebp2 (Mm01306292_m1), Dgat1 (Mm00515643_m1), Dgat2 (Mm00499536_m1), Scd1 (Mm00772290_m1), Pparg (Mm00440945_m1), Fasn (Mm00662319_m1), Gck (Mm00439129_m1), Pepck (Mm00440636_m1), G6pc (Mm00839363_m1), Vegf (Mm00437304_m1), Egf (Mm01316968_m1), Egfr (Mm00433023_m1), Glut1 (Mm00441473_m1), Myc (Mm00487804_m1), Bcl2 (Mm00477631_m1), Mcl1 (Mm01257352_g1), Ccl2 (Mm00441242_m1), Ccl7 (Mm00443113_m1), Mmp9 (Mm00442991_m1), Stat3 (Mm00456961_m1), Tbp (Mm00446973_m1). .. Caspase 3 activity was determined by measuring cleaved caspase 3 amounts of liver lysates by using PathScan Cleaved Caspase 3 ELISA kit (Cell Signaling) according to manufacturer's instructions.

    Article Title: Mechanisms of Sex Differences in TNFR2 Mediated Cardioprotection
    Article Snippet: .. 0.5 μg of total RNA was subjected to cDNA synthesis using cloned AMV first-strand cDNA synthesis kit (Invitrogen life technologies, Carlsbad, CA). cDNA from each sample was analyzed for 18S (assay ID# Hs99999901_s1), TNF (assay ID# Mm00443258_m1), IL-6 (assay ID# Mm00446190_m1), VEGFa (assay ID# Mm00437304_m1), SOCS3 (assay ID# Mm00545913_s1) and STAT3 (assay ID# Mm00456961_m1) by using TaqMan gene expression assay (Real-time PCR) (Applied Biosystems, Foster City, CA). ..

    Quantitative RT-PCR:

    Article Title: Visceral Endoderm Expression of Yin-Yang1 (YY1) Is Required for VEGFA Maintenance and Yolk Sac Development
    Article Snippet: .. Quantitative RT-PCR (qPCR) was performed using the following Taqman gene expression Assays: Yy1 (MM0456392_m1), VegfA (MM00437304_m1) and Hnf4α (MM00455964_m1). .. These assays were multiplexed with ActB using PerfeCTa® qPCR SuperMix, Low ROX™ (Quanta Biosciences).

    Polymerase Chain Reaction:

    Article Title: Developmental regression of hyaloid vasculature is triggered by neurons
    Article Snippet: Quantitative RT-PCR analysis Total RNA was prepared from retinal tissues, and reverse transcription was performed using Superscript II (Invitrogen). .. Quantitative PCR assays were conducted with a real-time PCR system (ABI 7500 Fast) using PCR master mix (TaqMan Fast Universal; Applied Biosystems) and a TaqMan gene expression assay mix of vegfa (Mm00437304_ml) and vegfr2 (Mm00440099_ml). .. A mouse β-actin (Mm00607939_s1) assay mix served as an endogenous control.

    Article Title: Therapeutic Efficacy of Topical Epigallocatechin Gallate (EGCG) in Murine Dry Eye
    Article Snippet: Total RNA was isolated from the dissected corneal tissue using Trizol (Invitrogen) and RNeasy Microkit (Qiagen). .. The first strand of complementary DNA (cDNA) was synthesized with random hexamers using SuperScript III™ reverse transcriptase (Invitrogen) and quantitative real-time polymerase chain reaction was performed using Taqman Universal PCR Mastermix and FAM-MGB dye-labeled predesigned primers (Applied Biosystems) for IL-1β (Mm00434228_m1), TNF-α (Mm99999068_m1), CCL2 (Mm00439620_m1), VEGF-A (Mm00437304_ml), VEGF-C (Mm00437313_ml), VEGF-D (Mm00438965_ml), and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) (Mm99999915_g1). ..

    Synthesized:

    Article Title: Therapeutic Efficacy of Topical Epigallocatechin Gallate (EGCG) in Murine Dry Eye
    Article Snippet: Total RNA was isolated from the dissected corneal tissue using Trizol (Invitrogen) and RNeasy Microkit (Qiagen). .. The first strand of complementary DNA (cDNA) was synthesized with random hexamers using SuperScript III™ reverse transcriptase (Invitrogen) and quantitative real-time polymerase chain reaction was performed using Taqman Universal PCR Mastermix and FAM-MGB dye-labeled predesigned primers (Applied Biosystems) for IL-1β (Mm00434228_m1), TNF-α (Mm99999068_m1), CCL2 (Mm00439620_m1), VEGF-A (Mm00437304_ml), VEGF-C (Mm00437313_ml), VEGF-D (Mm00438965_ml), and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) (Mm99999915_g1). ..

    Clone Assay:

    Article Title: Mechanisms of Sex Differences in TNFR2 Mediated Cardioprotection
    Article Snippet: .. 0.5 μg of total RNA was subjected to cDNA synthesis using cloned AMV first-strand cDNA synthesis kit (Invitrogen life technologies, Carlsbad, CA). cDNA from each sample was analyzed for 18S (assay ID# Hs99999901_s1), TNF (assay ID# Mm00443258_m1), IL-6 (assay ID# Mm00446190_m1), VEGFa (assay ID# Mm00437304_m1), SOCS3 (assay ID# Mm00545913_s1) and STAT3 (assay ID# Mm00456961_m1) by using TaqMan gene expression assay (Real-time PCR) (Applied Biosystems, Foster City, CA). ..

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  • 99
    Thermo Fisher gene exp vegfa mm00437304 m1
    DFP treatment decreases retinal markers of oxidative stress. Graph showing retinal isoprostane F2α-VI levels in the retinas of 9-month-old DKO mice treated with DFP for 5 months relative to untreated, age-matched DKO and WT mice ( A , n = 3 mice per group). DFP treatment significantly reduced Epo mRNA levels, as measured by qPCR, in the retinas of DKO mice treated for 6 months with DFP relative to untreated 9-month-old DKOs ( B , n = 3 mice per group). DFP did not change <t>Vegfa</t> mRNA levels in either neurosensory retina ( C ) or RPE/choroid ( D ), but it significantly reduced Cd68 mRNA levels in neurosensory retinas of treated DKO relative to untreated DKO controls ( E ) and showed a trend toward C3 mRNA level reduction in the RPE/choroid ( F ). *Significant difference ( P
    Gene Exp Vegfa Mm00437304 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DFP treatment decreases retinal markers of oxidative stress. Graph showing retinal isoprostane F2α-VI levels in the retinas of 9-month-old DKO mice treated with DFP for 5 months relative to untreated, age-matched DKO and WT mice ( A , n = 3 mice per group). DFP treatment significantly reduced Epo mRNA levels, as measured by qPCR, in the retinas of DKO mice treated for 6 months with DFP relative to untreated 9-month-old DKOs ( B , n = 3 mice per group). DFP did not change Vegfa mRNA levels in either neurosensory retina ( C ) or RPE/choroid ( D ), but it significantly reduced Cd68 mRNA levels in neurosensory retinas of treated DKO relative to untreated DKO controls ( E ) and showed a trend toward C3 mRNA level reduction in the RPE/choroid ( F ). *Significant difference ( P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: The Oral Iron Chelator Deferiprone Protects against Iron Overload–Induced Retinal Degeneration

    doi: 10.1167/iovs.10-6207

    Figure Lengend Snippet: DFP treatment decreases retinal markers of oxidative stress. Graph showing retinal isoprostane F2α-VI levels in the retinas of 9-month-old DKO mice treated with DFP for 5 months relative to untreated, age-matched DKO and WT mice ( A , n = 3 mice per group). DFP treatment significantly reduced Epo mRNA levels, as measured by qPCR, in the retinas of DKO mice treated for 6 months with DFP relative to untreated 9-month-old DKOs ( B , n = 3 mice per group). DFP did not change Vegfa mRNA levels in either neurosensory retina ( C ) or RPE/choroid ( D ), but it significantly reduced Cd68 mRNA levels in neurosensory retinas of treated DKO relative to untreated DKO controls ( E ) and showed a trend toward C3 mRNA level reduction in the RPE/choroid ( F ). *Significant difference ( P

    Article Snippet: Probes used were transferrin receptor ( Tfrc , Mm00441941_m1), vascular endothelial growth factor A ( Vegfa , Mm00437304_m1), erythropoietin ( Epo , Mm00433126_m1), CD68 antigen ( Cd68 , Mm03047343_m1*), and complement component 3 ( C3 , Mm01232779_m1).

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction

    Deletion of VEGF normalizes persistent hyaloid vessels in neuronal VEGFR2 knockout mice. (A) Quantitative PCR analysis for retinal tissues at P6 ( n = 4). (B) Representative immunoblots and quantification in three independent experiments. (C–O) Whole-mount staining of hyaloid vessels at P6 and quantification ( n = 4). Spontaneous endothelial apoptosis detected in control mice (arrowheads in K) is greatly reduced in Vegfr2 Δneuro mice and is recovered in Vegfr2 ; Vegfa Δneuro mice (arrowheads in M). (P) Schematic diagram depicting the transition of ocular circulatory systems. Bars: (C–G) 500 µm; (H–J) 200 µm; (K–M) 50 µm. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Developmental regression of hyaloid vasculature is triggered by neurons

    doi: 10.1084/jem.20151966

    Figure Lengend Snippet: Deletion of VEGF normalizes persistent hyaloid vessels in neuronal VEGFR2 knockout mice. (A) Quantitative PCR analysis for retinal tissues at P6 ( n = 4). (B) Representative immunoblots and quantification in three independent experiments. (C–O) Whole-mount staining of hyaloid vessels at P6 and quantification ( n = 4). Spontaneous endothelial apoptosis detected in control mice (arrowheads in K) is greatly reduced in Vegfr2 Δneuro mice and is recovered in Vegfr2 ; Vegfa Δneuro mice (arrowheads in M). (P) Schematic diagram depicting the transition of ocular circulatory systems. Bars: (C–G) 500 µm; (H–J) 200 µm; (K–M) 50 µm. *, P

    Article Snippet: Quantitative PCR assays were conducted with a real-time PCR system (ABI 7500 Fast) using PCR master mix (TaqMan Fast Universal; Applied Biosystems) and a TaqMan gene expression assay mix of vegfa (Mm00437304_ml) and vegfr2 (Mm00440099_ml).

    Techniques: Knock-Out, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot, Staining

    Inhibition of HDACs prevents hypoxia-induced increases in Vegfa expression in isolated cardiac myocytes. A , B ) Cells were pretreated with DMSO/TSA ( A ) or Nullscript/Scriptaid ( B ) for 1 h and exposed to 1% O 2 for 5 h. Vegfa expression levels were determined

    Journal:

    Article Title: Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice

    doi: 10.1096/fj.08-108548

    Figure Lengend Snippet: Inhibition of HDACs prevents hypoxia-induced increases in Vegfa expression in isolated cardiac myocytes. A , B ) Cells were pretreated with DMSO/TSA ( A ) or Nullscript/Scriptaid ( B ) for 1 h and exposed to 1% O 2 for 5 h. Vegfa expression levels were determined

    Article Snippet: Ten nanograms of cDNA was subjected to real-time PCR using Taqman gene expression assays (Applied Biosystems, Foster City, CA, USA) to assess the expression level of Vegfa (Mm00437304_m1), Hdac2 (Mm01193631_m1), Hdac3 (Mm01258403_gH), Hdac4 (Mm01299566_m1), Hdac5 (Mm00515917_m1), Hdac6 (Mm00515945_m1), Hdac9 (Mm00458456_m1), Egln1 (Mm00459770_m1), Egln2 (Mm00519067_m1), and Egln3 (Mm00472200_m1). β 2-microglobulin (Mm00437762_m1) was used as an internal control.

    Techniques: Inhibition, Expressing, Isolation

    Single-dose i.p. administration of HDACIs reduces I/R-induced Vegfa induction and vascular permeability in vivo . A ) Vegfa mRNA levels from the LV free wall of treated or control mice increase with ischemia, returning to baseline levels with single-dose

    Journal:

    Article Title: Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice

    doi: 10.1096/fj.08-108548

    Figure Lengend Snippet: Single-dose i.p. administration of HDACIs reduces I/R-induced Vegfa induction and vascular permeability in vivo . A ) Vegfa mRNA levels from the LV free wall of treated or control mice increase with ischemia, returning to baseline levels with single-dose

    Article Snippet: Ten nanograms of cDNA was subjected to real-time PCR using Taqman gene expression assays (Applied Biosystems, Foster City, CA, USA) to assess the expression level of Vegfa (Mm00437304_m1), Hdac2 (Mm01193631_m1), Hdac3 (Mm01258403_gH), Hdac4 (Mm01299566_m1), Hdac5 (Mm00515917_m1), Hdac6 (Mm00515945_m1), Hdac9 (Mm00458456_m1), Egln1 (Mm00459770_m1), Egln2 (Mm00519067_m1), and Egln3 (Mm00472200_m1). β 2-microglobulin (Mm00437762_m1) was used as an internal control.

    Techniques: Permeability, In Vivo, Mouse Assay

    HDAC4 plays a primary role in mediating the hypoxic response of Vegfa and Egln3 in isolated cardiac myocytes. A ) Cells were pretreated with Nullscript (N) or Scriptaid (S) for 1 h and exposed to 1% O 2 for 5 h. Quantitative RT-PCR analysis was performed

    Journal:

    Article Title: Histone deacetylase inhibition reduces myocardial ischemia-reperfusion injury in mice

    doi: 10.1096/fj.08-108548

    Figure Lengend Snippet: HDAC4 plays a primary role in mediating the hypoxic response of Vegfa and Egln3 in isolated cardiac myocytes. A ) Cells were pretreated with Nullscript (N) or Scriptaid (S) for 1 h and exposed to 1% O 2 for 5 h. Quantitative RT-PCR analysis was performed

    Article Snippet: Ten nanograms of cDNA was subjected to real-time PCR using Taqman gene expression assays (Applied Biosystems, Foster City, CA, USA) to assess the expression level of Vegfa (Mm00437304_m1), Hdac2 (Mm01193631_m1), Hdac3 (Mm01258403_gH), Hdac4 (Mm01299566_m1), Hdac5 (Mm00515917_m1), Hdac6 (Mm00515945_m1), Hdac9 (Mm00458456_m1), Egln1 (Mm00459770_m1), Egln2 (Mm00519067_m1), and Egln3 (Mm00472200_m1). β 2-microglobulin (Mm00437762_m1) was used as an internal control.

    Techniques: Isolation, Quantitative RT-PCR