gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Deletion of Notch3 Impairs Contractility of Renal Resistance Vessels Due to Deficient Ca 2+ Entry"

    Article Title: Deletion of Notch3 Impairs Contractility of Renal Resistance Vessels Due to Deficient Ca 2+ Entry

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232416068

    Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).
    Figure Legend Snippet: Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ryr2 mm00465877 m1/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
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    gene exp ryr2 mm00465877 m1 - by Bioz Stars, 2024-05
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    Images

    1) Product Images from "Deletion of Notch3 Impairs Contractility of Renal Resistance Vessels Due to Deficient Ca 2+ Entry"

    Article Title: Deletion of Notch3 Impairs Contractility of Renal Resistance Vessels Due to Deficient Ca 2+ Entry

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232416068

    Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).
    Figure Legend Snippet: Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    The effect of hypoxia and Rycal treatment on <t>RyR2</t> and SERCA2a gene expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a gene expression. n = 6, * P < .050 for comparison with 1% O 2 , # P < .050 for comparison with Vehicle, $ P < .050 for comparison with 0.3 mM (all 1-way ANOVA with Tukey post hoc test).
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes"

    Article Title: Hypoxia-Induced Sarcoplasmic Reticulum Ca 2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes

    Journal: Anatolian Journal of Cardiology

    doi: 10.5152/AnatolJCardiol.2022.1223

    The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a gene expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a gene expression. n = 6, * P < .050 for comparison with 1% O 2 , # P < .050 for comparison with Vehicle, $ P < .050 for comparison with 0.3 mM (all 1-way ANOVA with Tukey post hoc test).
    Figure Legend Snippet: The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a gene expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a gene expression. n = 6, * P < .050 for comparison with 1% O 2 , # P < .050 for comparison with Vehicle, $ P < .050 for comparison with 0.3 mM (all 1-way ANOVA with Tukey post hoc test).

    Techniques Used: Expressing

    The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a protein expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a protein expression. n = 6. No statistically significant comparison.
    Figure Legend Snippet: The effect of hypoxia and Rycal treatment on RyR2 and SERCA2a protein expression. The effect of 7-day hypoxic and normoxic exposure with Rycals/Vehicle (DMSO) treatment on RyR2 and SERCA2a protein expression. n = 6. No statistically significant comparison.

    Techniques Used: Expressing

    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Cbx1, PurB, and Sp3 bind to cardiomyocyte-specific genes in fibroblasts. A , chromatin derived from cardiac fibroblasts was incubated with antibodies for Cbx1, PurB, or Sp3. An isotype antibody was used as a control. Immunoprecipitated DNA was analyzed by high-throughput sequencing. Bioinformatic approaches were used to determine Cbx1-, PurB-, and Sp3-binding sites. The Venn diagram details the number of genes with Cbx1-, PurB-, and Sp3-binding sites. B , Cbx1-, PurB-, and Sp3-binding sites in the cardiomyocyte-specific genes Ttn, <t>Ryr2,</t> and Kcnj6. C , Cbx1-, PurB-, and Sp3-binding peaks in the Nebl gene. D , Gene Ontology analysis of the genes to which Cbx1, PurB, and Sp3 were bound.
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes"

    Article Title: A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2022.102053

    Cbx1, PurB, and Sp3 bind to cardiomyocyte-specific genes in fibroblasts. A , chromatin derived from cardiac fibroblasts was incubated with antibodies for Cbx1, PurB, or Sp3. An isotype antibody was used as a control. Immunoprecipitated DNA was analyzed by high-throughput sequencing. Bioinformatic approaches were used to determine Cbx1-, PurB-, and Sp3-binding sites. The Venn diagram details the number of genes with Cbx1-, PurB-, and Sp3-binding sites. B , Cbx1-, PurB-, and Sp3-binding sites in the cardiomyocyte-specific genes Ttn, Ryr2, and Kcnj6. C , Cbx1-, PurB-, and Sp3-binding peaks in the Nebl gene. D , Gene Ontology analysis of the genes to which Cbx1, PurB, and Sp3 were bound.
    Figure Legend Snippet: Cbx1, PurB, and Sp3 bind to cardiomyocyte-specific genes in fibroblasts. A , chromatin derived from cardiac fibroblasts was incubated with antibodies for Cbx1, PurB, or Sp3. An isotype antibody was used as a control. Immunoprecipitated DNA was analyzed by high-throughput sequencing. Bioinformatic approaches were used to determine Cbx1-, PurB-, and Sp3-binding sites. The Venn diagram details the number of genes with Cbx1-, PurB-, and Sp3-binding sites. B , Cbx1-, PurB-, and Sp3-binding sites in the cardiomyocyte-specific genes Ttn, Ryr2, and Kcnj6. C , Cbx1-, PurB-, and Sp3-binding peaks in the Nebl gene. D , Gene Ontology analysis of the genes to which Cbx1, PurB, and Sp3 were bound.

    Techniques Used: Derivative Assay, Incubation, Immunoprecipitation, Next-Generation Sequencing, Binding Assay

    Cbx1, PurB, and Sp3 regulate chromatin architecture. Cardiac fibroblasts were transfected with siRNAs targeting Cbx1, PurB, or Sp3. A nontargeting siRNA was used as a control. After 7 days, chromatin was isolated and digested with micrococcal nuclease (MNase). Following MNase digestion, the resulting undigested DNA was submitted for high-throughput sequencing (MNase-Seq) and mapped to the mouse genome. A , MNase digestion was optimized to give rise to one nucleosome. Read lengths were analyzed after sequencing and summed. As expected, the majority of read lengths were 1 nucleosome is size (∼150 bp). B , MNase accessibility signals around transcription start sites (TSSs). The y -axis represents the read number for each 10 bp bin normalized to the effective genome size for the mouse. C , nucleosomes ( black bars ) were plotted on the cardiomyocyte-specific genes Ryr2 and Actn2 . D , nucleosomes in noncardiomyocyte genes.
    Figure Legend Snippet: Cbx1, PurB, and Sp3 regulate chromatin architecture. Cardiac fibroblasts were transfected with siRNAs targeting Cbx1, PurB, or Sp3. A nontargeting siRNA was used as a control. After 7 days, chromatin was isolated and digested with micrococcal nuclease (MNase). Following MNase digestion, the resulting undigested DNA was submitted for high-throughput sequencing (MNase-Seq) and mapped to the mouse genome. A , MNase digestion was optimized to give rise to one nucleosome. Read lengths were analyzed after sequencing and summed. As expected, the majority of read lengths were 1 nucleosome is size (∼150 bp). B , MNase accessibility signals around transcription start sites (TSSs). The y -axis represents the read number for each 10 bp bin normalized to the effective genome size for the mouse. C , nucleosomes ( black bars ) were plotted on the cardiomyocyte-specific genes Ryr2 and Actn2 . D , nucleosomes in noncardiomyocyte genes.

    Techniques Used: Transfection, Isolation, Next-Generation Sequencing, Sequencing

    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    (A) mRNAs related to mitochondrial dynamics (n = 4). (B) Chop mRNA (n = 4). (C) Pgc-1α , Nrf1 , and Tfam mRNAs (n = 4). (D) Atp2a3 , <t>Ryr2</t> , and Itpr2 mRNAs (n = 4). (E, F) Mitochondrial membrane potential determined by TMRE staining (n = 4). (G) Intracellular ATP level (n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001.
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Neuromedin U uses Gα i2 and Gα o to suppress glucose-stimulated Ca 2+ signaling and insulin secretion in pancreatic β cells"

    Article Title: Neuromedin U uses Gα i2 and Gα o to suppress glucose-stimulated Ca 2+ signaling and insulin secretion in pancreatic β cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0250232

    (A) mRNAs related to mitochondrial dynamics (n = 4). (B) Chop mRNA (n = 4). (C) Pgc-1α , Nrf1 , and Tfam mRNAs (n = 4). (D) Atp2a3 , Ryr2 , and Itpr2 mRNAs (n = 4). (E, F) Mitochondrial membrane potential determined by TMRE staining (n = 4). (G) Intracellular ATP level (n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: (A) mRNAs related to mitochondrial dynamics (n = 4). (B) Chop mRNA (n = 4). (C) Pgc-1α , Nrf1 , and Tfam mRNAs (n = 4). (D) Atp2a3 , Ryr2 , and Itpr2 mRNAs (n = 4). (E, F) Mitochondrial membrane potential determined by TMRE staining (n = 4). (G) Intracellular ATP level (n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Staining

    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    ( A ) <t>Ryr1</t> , <t>Ryr2</t> and <t>Ryr3</t> expression in lysates prepared from whole mammary tissue (including luminal, basal and stromal cells) dissected from virgin or lactating animals (n = 4 mice). ( B ) Krt14 , Esr1 and Ryr1 levels in freshly sorted luminal and basal cells (n = 3 mice). Graphs show mean ± SEM; * P < 0.05 (Student’s t-test); n.d., not detected. Ryr2 and Ryr3 transcripts were either not detected or detected at very low levels in only a fraction of samples from both luminal and basal cells.
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Multiscale activity imaging in mammary gland reveals how oxytocin enables lactation"

    Article Title: Multiscale activity imaging in mammary gland reveals how oxytocin enables lactation

    Journal: bioRxiv

    doi: 10.1101/657510

    ( A ) Ryr1 , Ryr2 and Ryr3 expression in lysates prepared from whole mammary tissue (including luminal, basal and stromal cells) dissected from virgin or lactating animals (n = 4 mice). ( B ) Krt14 , Esr1 and Ryr1 levels in freshly sorted luminal and basal cells (n = 3 mice). Graphs show mean ± SEM; * P < 0.05 (Student’s t-test); n.d., not detected. Ryr2 and Ryr3 transcripts were either not detected or detected at very low levels in only a fraction of samples from both luminal and basal cells.
    Figure Legend Snippet: ( A ) Ryr1 , Ryr2 and Ryr3 expression in lysates prepared from whole mammary tissue (including luminal, basal and stromal cells) dissected from virgin or lactating animals (n = 4 mice). ( B ) Krt14 , Esr1 and Ryr1 levels in freshly sorted luminal and basal cells (n = 3 mice). Graphs show mean ± SEM; * P < 0.05 (Student’s t-test); n.d., not detected. Ryr2 and Ryr3 transcripts were either not detected or detected at very low levels in only a fraction of samples from both luminal and basal cells.

    Techniques Used: Expressing

    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Cardiac reprogramming is more efficient using pMX-GMT (a Mixture of pMX-Gata4, -Mef2c, and -Tbx5) than that using pDox-GMT (a Mixture of pDox-Gata4, -Mef2c, and -Tbx5). ( A ) qRT-PCR for Gata4, Mef2c, and Tbx5 expression in mouse embryonic fibroblasts (MEFs), pDox-GMT-transduced MEFs, pMX-GMT-transduced MEFs, and heart tissue ( n = 3, independent triplicate experiments). Data were normalized to the values of heart tissue (the dash lines); ( B ) qRT-PCR for TnnT2, Myh6, Nppa, and <t>Ryr2</t> mRNA expression in mouse embryonic fibroblasts (MEFs), pDox-GMT-transduced MEFs and pMX-GMT-transduced MEFs after one week ( n = 3 independent triplicate experiments). Data were normalized to the values of pDox-GMT-transduced MEFs (the dash lines); ( C ) FACS analyses of cardiac troponin T (cTnT) expression induced by pDox-GMT in the presence of Dox or pMX-GMT after one week; ( D ) quantitative data of ( C ) ( n = 3 independent triplicate experiments); ( E ) immunocytochemistry for α-actinin in pDox-GMT-transduced MEFs in the presence of Dox, and pMX-GMT-transduced MEFs, after four weeks. The high-magnification views in the insets show the sarcomeric organization; ( F ) quantitation of α-actinin-positive cells ( n = 3 independent triplicate experiments). All data are presented as mean ± SD. * p < 0.05, ** p < 0.01 vs. the relevant control. The scale bars represent 100 µm.
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression"

    Article Title: Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18081805

    Cardiac reprogramming is more efficient using pMX-GMT (a Mixture of pMX-Gata4, -Mef2c, and -Tbx5) than that using pDox-GMT (a Mixture of pDox-Gata4, -Mef2c, and -Tbx5). ( A ) qRT-PCR for Gata4, Mef2c, and Tbx5 expression in mouse embryonic fibroblasts (MEFs), pDox-GMT-transduced MEFs, pMX-GMT-transduced MEFs, and heart tissue ( n = 3, independent triplicate experiments). Data were normalized to the values of heart tissue (the dash lines); ( B ) qRT-PCR for TnnT2, Myh6, Nppa, and Ryr2 mRNA expression in mouse embryonic fibroblasts (MEFs), pDox-GMT-transduced MEFs and pMX-GMT-transduced MEFs after one week ( n = 3 independent triplicate experiments). Data were normalized to the values of pDox-GMT-transduced MEFs (the dash lines); ( C ) FACS analyses of cardiac troponin T (cTnT) expression induced by pDox-GMT in the presence of Dox or pMX-GMT after one week; ( D ) quantitative data of ( C ) ( n = 3 independent triplicate experiments); ( E ) immunocytochemistry for α-actinin in pDox-GMT-transduced MEFs in the presence of Dox, and pMX-GMT-transduced MEFs, after four weeks. The high-magnification views in the insets show the sarcomeric organization; ( F ) quantitation of α-actinin-positive cells ( n = 3 independent triplicate experiments). All data are presented as mean ± SD. * p < 0.05, ** p < 0.01 vs. the relevant control. The scale bars represent 100 µm.
    Figure Legend Snippet: Cardiac reprogramming is more efficient using pMX-GMT (a Mixture of pMX-Gata4, -Mef2c, and -Tbx5) than that using pDox-GMT (a Mixture of pDox-Gata4, -Mef2c, and -Tbx5). ( A ) qRT-PCR for Gata4, Mef2c, and Tbx5 expression in mouse embryonic fibroblasts (MEFs), pDox-GMT-transduced MEFs, pMX-GMT-transduced MEFs, and heart tissue ( n = 3, independent triplicate experiments). Data were normalized to the values of heart tissue (the dash lines); ( B ) qRT-PCR for TnnT2, Myh6, Nppa, and Ryr2 mRNA expression in mouse embryonic fibroblasts (MEFs), pDox-GMT-transduced MEFs and pMX-GMT-transduced MEFs after one week ( n = 3 independent triplicate experiments). Data were normalized to the values of pDox-GMT-transduced MEFs (the dash lines); ( C ) FACS analyses of cardiac troponin T (cTnT) expression induced by pDox-GMT in the presence of Dox or pMX-GMT after one week; ( D ) quantitative data of ( C ) ( n = 3 independent triplicate experiments); ( E ) immunocytochemistry for α-actinin in pDox-GMT-transduced MEFs in the presence of Dox, and pMX-GMT-transduced MEFs, after four weeks. The high-magnification views in the insets show the sarcomeric organization; ( F ) quantitation of α-actinin-positive cells ( n = 3 independent triplicate experiments). All data are presented as mean ± SD. * p < 0.05, ** p < 0.01 vs. the relevant control. The scale bars represent 100 µm.

    Techniques Used: Quantitative RT-PCR, Expressing, Immunocytochemistry, Quantitation Assay

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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp ryr2 mm00465877 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Calcium handling in isolated myocytes. Cardiomyocytes isolated from chronic kidney disease (CKD) mice at 8 weeks have shorter resting sarcomere length (A upper) but no appreciable accumulation of diastolic calcium (A lower). Measurements are from individual cells isolated from 3 to 4 mice per group are presented and are reflective of three separate experiments with comparative results. Hearts were collected at 8 and 16 weeks following surgery ( n = 7–10 per group per time‐point) and mRNA expression quantified using qRT‐PCR for genes encoding proteins involved in sarcolemmal calcium handling (B). Atp2a2 (sarcoplasmic reticulum Ca 2+ ATPase, SERCA2a), Slc8a1 (sodium/calcium exchanger, NCX), Pln (phospholamban), and <t>Ryr2</t> (ryanodine receptor 2). ** P < 0.01 between Sham and CKD.
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Myocardial dysfunction occurs prior to changes in ventricular geometry in mice with chronic kidney disease ( CKD )"

    Article Title: Myocardial dysfunction occurs prior to changes in ventricular geometry in mice with chronic kidney disease ( CKD )

    Journal: Physiological Reports

    doi: 10.14814/phy2.12732

    Calcium handling in isolated myocytes. Cardiomyocytes isolated from chronic kidney disease (CKD) mice at 8 weeks have shorter resting sarcomere length (A upper) but no appreciable accumulation of diastolic calcium (A lower). Measurements are from individual cells isolated from 3 to 4 mice per group are presented and are reflective of three separate experiments with comparative results. Hearts were collected at 8 and 16 weeks following surgery ( n = 7–10 per group per time‐point) and mRNA expression quantified using qRT‐PCR for genes encoding proteins involved in sarcolemmal calcium handling (B). Atp2a2 (sarcoplasmic reticulum Ca 2+ ATPase, SERCA2a), Slc8a1 (sodium/calcium exchanger, NCX), Pln (phospholamban), and Ryr2 (ryanodine receptor 2). ** P < 0.01 between Sham and CKD.
    Figure Legend Snippet: Calcium handling in isolated myocytes. Cardiomyocytes isolated from chronic kidney disease (CKD) mice at 8 weeks have shorter resting sarcomere length (A upper) but no appreciable accumulation of diastolic calcium (A lower). Measurements are from individual cells isolated from 3 to 4 mice per group are presented and are reflective of three separate experiments with comparative results. Hearts were collected at 8 and 16 weeks following surgery ( n = 7–10 per group per time‐point) and mRNA expression quantified using qRT‐PCR for genes encoding proteins involved in sarcolemmal calcium handling (B). Atp2a2 (sarcoplasmic reticulum Ca 2+ ATPase, SERCA2a), Slc8a1 (sodium/calcium exchanger, NCX), Pln (phospholamban), and Ryr2 (ryanodine receptor 2). ** P < 0.01 between Sham and CKD.

    Techniques Used: Isolation, Expressing, Quantitative RT-PCR

    Primers used in qRT‐PCR experiments
    Figure Legend Snippet: Primers used in qRT‐PCR experiments

    Techniques Used: TaqMan Assay

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    Thermo Fisher gene exp ryr2 mm00465877 m1
    Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).
    Gene Exp Ryr2 Mm00465877 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ryr2 mm00465877 m1/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ryr2 mm00465877 m1 - by Bioz Stars, 2024-05
    93/100 stars
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    Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).

    Journal: International Journal of Molecular Sciences

    Article Title: Deletion of Notch3 Impairs Contractility of Renal Resistance Vessels Due to Deficient Ca 2+ Entry

    doi: 10.3390/ijms232416068

    Figure Lengend Snippet: Comparative real-time PCR mRNA expression of 47 genes related to contractile function in the afferent arterioles from Notch3 −/− and wild-type littermates. Note that the expression of Cacna1h coding the α 1H subunit of the T-type Ca 2+ channel (Ca v 3.2) gene was strongly downregulated in Notch3 −/− ( p < 0.001 vs. wild-type). In contrast, Cacna1c coding the α 1C subunit of the L-type Ca 2+ channel was similarly expressed in the two strains (No. 26 on the list).

    Article Snippet: 20 , ryanodine receptor 2 , ryr2 , Ryr2-Mm00465877_m1 , 1.13 ± 0.16 , 0.85 ± 0.1 , −0.42 , 0.14.

    Techniques: Real-time Polymerase Chain Reaction, Expressing