gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr1 hs00166991 m1
    List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study"

    Article Title: Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00500

    List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).
    Figure Legend Snippet: List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).

    Techniques Used: Real-time Polymerase Chain Reaction, Histone Deacetylase Assay

    Real-time PCR analysis of mRNA expression level of genes involved in ClC-1 chloride channel regulation and calcium signaling in VL muscle biopsies of patients in therapy with statin showing sign of myopathy. Histograms show quantification of transcript levels for MEF2D, HDAC5, CN, NFix, RyR1, SERCA1, Troponin T and Parvalbumin normalized by the beta-2 microglobulin gene. In each graph, the bars represent the mean values ± SEM in myopathic subjects (Statin) compared with those of untreated subjects (Ctrl) from the number of subjects indicated in brackets. ∗ Significantly different with respect to the own control ( P < 0.05 or less) by Student’s t -test.
    Figure Legend Snippet: Real-time PCR analysis of mRNA expression level of genes involved in ClC-1 chloride channel regulation and calcium signaling in VL muscle biopsies of patients in therapy with statin showing sign of myopathy. Histograms show quantification of transcript levels for MEF2D, HDAC5, CN, NFix, RyR1, SERCA1, Troponin T and Parvalbumin normalized by the beta-2 microglobulin gene. In each graph, the bars represent the mean values ± SEM in myopathic subjects (Statin) compared with those of untreated subjects (Ctrl) from the number of subjects indicated in brackets. ∗ Significantly different with respect to the own control ( P < 0.05 or less) by Student’s t -test.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp ryr1 hs00166991 m1
    List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ryr1 hs00166991 m1/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ryr1 hs00166991 m1 - by Bioz Stars, 2024-05
    89/100 stars

    Images

    1) Product Images from "Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study"

    Article Title: Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00500

    List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).
    Figure Legend Snippet: List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).

    Techniques Used: Real-time Polymerase Chain Reaction, Histone Deacetylase Assay

    Real-time PCR analysis of mRNA expression level of genes involved in ClC-1 chloride channel regulation and calcium signaling in VL muscle biopsies of patients in therapy with statin showing sign of myopathy. Histograms show quantification of transcript levels for MEF2D, HDAC5, CN, NFix, RyR1, SERCA1, Troponin T and Parvalbumin normalized by the beta-2 microglobulin gene. In each graph, the bars represent the mean values ± SEM in myopathic subjects (Statin) compared with those of untreated subjects (Ctrl) from the number of subjects indicated in brackets. ∗ Significantly different with respect to the own control ( P < 0.05 or less) by Student’s t -test.
    Figure Legend Snippet: Real-time PCR analysis of mRNA expression level of genes involved in ClC-1 chloride channel regulation and calcium signaling in VL muscle biopsies of patients in therapy with statin showing sign of myopathy. Histograms show quantification of transcript levels for MEF2D, HDAC5, CN, NFix, RyR1, SERCA1, Troponin T and Parvalbumin normalized by the beta-2 microglobulin gene. In each graph, the bars represent the mean values ± SEM in myopathic subjects (Statin) compared with those of untreated subjects (Ctrl) from the number of subjects indicated in brackets. ∗ Significantly different with respect to the own control ( P < 0.05 or less) by Student’s t -test.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr1 hs00166991 m1
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ryr1 hs00166991 m1/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    89/100 stars

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    gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp ryr1 hs00166991 m1
    Secreted proteins such as collagen are synthesized within the ER, which contains the major intracellular Ca 2+ store. In response to extracellular stimuli, Ca 2+ is released from the ER lumen into the cytoplasm via IP 3 and <t>ryanodine</t> <t>receptors.</t> Cytoplasmic Ca 2+ that does not bind signaling proteins involved in gene expression is transported back into the ER via SERCA pumps. The continuous fluctuations in free [Ca 2+ ] to and from the ER are indirectly regulated by TRIC channel activity, which mediates transmembrane K + flux to maintain electroneutrality across the ER membrane. In fibroblasts and osteoblasts, ER-resident Ca 2+ -binding chaperones, including BiP, CyPB, PDI and calreticulin (CRT), directly interact with collagen alpha chains (red polypeptides) to facilitate or catalyze specific modifications required for assembly and folding. In the absence of TRIC-mediated K + flux, Ca2 + -dependent “cycling” of these chaperones between inactive, sequestered forms and active, substrate-interacting forms, is dysregulated. Thus, absence of TRIC-B alters ER luminal [Ca 2+ ], affecting synthesis and secretion of collagen, as well as interactions of collagen-specific chaperones with each other and with their substrate, resulting in abnormal collagen assembly and post-translational modification. BiP, GRP78; CyPB, cyclophilin B; CRT, calreticulin; IP3R, inositol triphosphate receptor; LH1, lysyl hydroxylase 1; PDI, protein disulfide isomerase; SERCA2b, sarcoplasmic-endoplasmic reticulum Ca 2+ -ATPase isoform 2b; TRIC-B, trimeric intracellular cation channel subtype B.
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ryr1 hs00166991 m1/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
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    1) Product Images from "Absence of the ER Cation Channel TMEM38B /TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta"

    Article Title: Absence of the ER Cation Channel TMEM38B /TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1006156

    Secreted proteins such as collagen are synthesized within the ER, which contains the major intracellular Ca 2+ store. In response to extracellular stimuli, Ca 2+ is released from the ER lumen into the cytoplasm via IP 3 and ryanodine receptors. Cytoplasmic Ca 2+ that does not bind signaling proteins involved in gene expression is transported back into the ER via SERCA pumps. The continuous fluctuations in free [Ca 2+ ] to and from the ER are indirectly regulated by TRIC channel activity, which mediates transmembrane K + flux to maintain electroneutrality across the ER membrane. In fibroblasts and osteoblasts, ER-resident Ca 2+ -binding chaperones, including BiP, CyPB, PDI and calreticulin (CRT), directly interact with collagen alpha chains (red polypeptides) to facilitate or catalyze specific modifications required for assembly and folding. In the absence of TRIC-mediated K + flux, Ca2 + -dependent “cycling” of these chaperones between inactive, sequestered forms and active, substrate-interacting forms, is dysregulated. Thus, absence of TRIC-B alters ER luminal [Ca 2+ ], affecting synthesis and secretion of collagen, as well as interactions of collagen-specific chaperones with each other and with their substrate, resulting in abnormal collagen assembly and post-translational modification. BiP, GRP78; CyPB, cyclophilin B; CRT, calreticulin; IP3R, inositol triphosphate receptor; LH1, lysyl hydroxylase 1; PDI, protein disulfide isomerase; SERCA2b, sarcoplasmic-endoplasmic reticulum Ca 2+ -ATPase isoform 2b; TRIC-B, trimeric intracellular cation channel subtype B.
    Figure Legend Snippet: Secreted proteins such as collagen are synthesized within the ER, which contains the major intracellular Ca 2+ store. In response to extracellular stimuli, Ca 2+ is released from the ER lumen into the cytoplasm via IP 3 and ryanodine receptors. Cytoplasmic Ca 2+ that does not bind signaling proteins involved in gene expression is transported back into the ER via SERCA pumps. The continuous fluctuations in free [Ca 2+ ] to and from the ER are indirectly regulated by TRIC channel activity, which mediates transmembrane K + flux to maintain electroneutrality across the ER membrane. In fibroblasts and osteoblasts, ER-resident Ca 2+ -binding chaperones, including BiP, CyPB, PDI and calreticulin (CRT), directly interact with collagen alpha chains (red polypeptides) to facilitate or catalyze specific modifications required for assembly and folding. In the absence of TRIC-mediated K + flux, Ca2 + -dependent “cycling” of these chaperones between inactive, sequestered forms and active, substrate-interacting forms, is dysregulated. Thus, absence of TRIC-B alters ER luminal [Ca 2+ ], affecting synthesis and secretion of collagen, as well as interactions of collagen-specific chaperones with each other and with their substrate, resulting in abnormal collagen assembly and post-translational modification. BiP, GRP78; CyPB, cyclophilin B; CRT, calreticulin; IP3R, inositol triphosphate receptor; LH1, lysyl hydroxylase 1; PDI, protein disulfide isomerase; SERCA2b, sarcoplasmic-endoplasmic reticulum Ca 2+ -ATPase isoform 2b; TRIC-B, trimeric intracellular cation channel subtype B.

    Techniques Used: Synthesized, Expressing, Activity Assay, Binding Assay, Modification

    gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr1 hs00166991 m1
    List genes used on low density RT‐PCR array microfludic cards (Applied Biosystems Inc., Foster City, CA, USA)
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Statin myalgia is not associated with reduced muscle strength, mass or protein turnover in older male volunteers, but is allied with a slowing of time to peak power output, insulin resistance and differential muscle mRNA expression"

    Article Title: Statin myalgia is not associated with reduced muscle strength, mass or protein turnover in older male volunteers, but is allied with a slowing of time to peak power output, insulin resistance and differential muscle mRNA expression

    Journal: The Journal of Physiology

    doi: 10.1113/jphysiol.2014.285577

    List genes used on low density RT‐PCR array microfludic cards (Applied Biosystems Inc., Foster City, CA, USA)
    Figure Legend Snippet: List genes used on low density RT‐PCR array microfludic cards (Applied Biosystems Inc., Foster City, CA, USA)

    Techniques Used: Binding Assay

    gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr1 hs00166991 m1
    Genes Selected for Molecular Analysis
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 1 article reviews
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    1) Product Images from "Statin Therapy and the Expression of Genes that Regulate Calcium Homeostasis and Membrane Repair in Skeletal Muscle"

    Article Title: Statin Therapy and the Expression of Genes that Regulate Calcium Homeostasis and Membrane Repair in Skeletal Muscle

    Journal:

    doi: 10.2353/ajpath.2010.091140

    Genes Selected for Molecular Analysis
    Figure Legend Snippet: Genes Selected for Molecular Analysis

    Techniques Used: Binding Assay

    Changes in the gene expression (mRNA) levels of key marker proteins within the skeletal muscle biopsies of non-myopathic patients undergoing statin therapy. A: Color-coded display of the two categories of patients whose biopsies were analyzed for the mRNA levels of genes encoding proteins indicated in B–E. These patients included the 20 controls who had never been treated with statins (pink) and the 19 asymptomatic ones who were receiving statin therapy (red). B–E: The mRNA expression levels of 41 genes were determined using a custom Taqman microarray system. The symbols (color-coded as in a) depict the Fold Differences [reference group: subjects who had never received statin therapy (pink)] for each gene product, normalized to the level of 18S RNA (mean values ± SEM). B: Most of the genes implicated in Ca2+ homeostasis are up-regulated (ITPR1&2&3, RYR1&3, ATPA2&3, CACNA1, ATP2B1). Several proteases (calpains 1&3; CAPN1&3) are up-regulated. C: Genes encoding structural proteins of the T-tubular system: members of the annexin family (ANXA1&2&4&6, S100A6, S100A10), for dysferlin (DYSF) and for dystrophin (DMD), are highly significantly up-regulated. D: Cholesterol-sensors SREBP cleavage-activating protein (SCAP) and the HMG CoA reductase (HMGCR), as well as the LDL receptor (LDLR) are highly significantly up-regulated. E: Genes that are often associated with the remodeling of muscle fibers (FGFR4, COL1A1, and FBXO32), or with mitochondrial activity (MT-CO1 and MT-ND1 and MT-ATP6), or actin (ACATA1) are not significantly up-regulated. For identification of gene products and P values see Table 1.
    Figure Legend Snippet: Changes in the gene expression (mRNA) levels of key marker proteins within the skeletal muscle biopsies of non-myopathic patients undergoing statin therapy. A: Color-coded display of the two categories of patients whose biopsies were analyzed for the mRNA levels of genes encoding proteins indicated in B–E. These patients included the 20 controls who had never been treated with statins (pink) and the 19 asymptomatic ones who were receiving statin therapy (red). B–E: The mRNA expression levels of 41 genes were determined using a custom Taqman microarray system. The symbols (color-coded as in a) depict the Fold Differences [reference group: subjects who had never received statin therapy (pink)] for each gene product, normalized to the level of 18S RNA (mean values ± SEM). B: Most of the genes implicated in Ca2+ homeostasis are up-regulated (ITPR1&2&3, RYR1&3, ATPA2&3, CACNA1, ATP2B1). Several proteases (calpains 1&3; CAPN1&3) are up-regulated. C: Genes encoding structural proteins of the T-tubular system: members of the annexin family (ANXA1&2&4&6, S100A6, S100A10), for dysferlin (DYSF) and for dystrophin (DMD), are highly significantly up-regulated. D: Cholesterol-sensors SREBP cleavage-activating protein (SCAP) and the HMG CoA reductase (HMGCR), as well as the LDL receptor (LDLR) are highly significantly up-regulated. E: Genes that are often associated with the remodeling of muscle fibers (FGFR4, COL1A1, and FBXO32), or with mitochondrial activity (MT-CO1 and MT-ND1 and MT-ATP6), or actin (ACATA1) are not significantly up-regulated. For identification of gene products and P values see Table 1.

    Techniques Used: Expressing, Marker, Microarray, Activity Assay

    Large-scale normalization of gene expression after discontinuation of statin therapy. A: Color-coded display of the two categories of patients whose biopsies were analyzed for the mRNA levels of genes encoding proteins indicated in B–E. These patients included the 20 control individuals who had never undergone statin therapy (pale blue) and the 14 myopathic ones who had discontinued their course of treatment with statins (turquoise). B–E: The mRNA levels of 41 candidate genes were determined using a custom TaqMan microarray. These were normalized to the level of 18S RNA. The symbols (color-coded as in A) depict fold differences [reference group: subjects who had never received statin therapy (pale blue)] for each gene product (mean values ± SEM). B: Several genes that are implicated in Ca2+ homeostasis remain up-regulated: ryanodine receptor 3 (RYR3), the sarco-endoplasmic ATPase 3 (ATP2B3) and calpain 3 (CAPN3). C: Genes that are associated with T-tubular structure and membrane repair are no longer up-regulated, whereas the structural marker of the sarcolemma (dystrophin: DMD) remains highly significantly elevated. D: The gene expression levels of the cholesterol-sensors (SCAP, HMGCR) and the LDL recptor (LDLR) have returned to control values. E: The genes for NADH 1 (MT-ND1) and the mitochondrial ATPase 6 (MT-ATP6) are significantly down-regulated. For identification of gene products and P values see Table 1.
    Figure Legend Snippet: Large-scale normalization of gene expression after discontinuation of statin therapy. A: Color-coded display of the two categories of patients whose biopsies were analyzed for the mRNA levels of genes encoding proteins indicated in B–E. These patients included the 20 control individuals who had never undergone statin therapy (pale blue) and the 14 myopathic ones who had discontinued their course of treatment with statins (turquoise). B–E: The mRNA levels of 41 candidate genes were determined using a custom TaqMan microarray. These were normalized to the level of 18S RNA. The symbols (color-coded as in A) depict fold differences [reference group: subjects who had never received statin therapy (pale blue)] for each gene product (mean values ± SEM). B: Several genes that are implicated in Ca2+ homeostasis remain up-regulated: ryanodine receptor 3 (RYR3), the sarco-endoplasmic ATPase 3 (ATP2B3) and calpain 3 (CAPN3). C: Genes that are associated with T-tubular structure and membrane repair are no longer up-regulated, whereas the structural marker of the sarcolemma (dystrophin: DMD) remains highly significantly elevated. D: The gene expression levels of the cholesterol-sensors (SCAP, HMGCR) and the LDL recptor (LDLR) have returned to control values. E: The genes for NADH 1 (MT-ND1) and the mitochondrial ATPase 6 (MT-ATP6) are significantly down-regulated. For identification of gene products and P values see Table 1.

    Techniques Used: Expressing, Microarray, Marker

    gene exp ryr1 hs00166991 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ryr1 hs00166991 m1
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp ryr1 hs00166991 m1
    List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).
    Gene Exp Ryr1 Hs00166991 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ryr1 hs00166991 m1/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ryr1 hs00166991 m1 - by Bioz Stars, 2024-05
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    List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).

    Journal: Frontiers in Pharmacology

    Article Title: Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study

    doi: 10.3389/fphar.2017.00500

    Figure Lengend Snippet: List of genes analyzed and quantified in human muscle biopsies by real-time PCR (Applied Biosystem).

    Article Snippet: Ryr1 , Ryanodine receptor 1 (skeletal) , Hs00166991_m1.

    Techniques: Real-time Polymerase Chain Reaction, Histone Deacetylase Assay

    Real-time PCR analysis of mRNA expression level of genes involved in ClC-1 chloride channel regulation and calcium signaling in VL muscle biopsies of patients in therapy with statin showing sign of myopathy. Histograms show quantification of transcript levels for MEF2D, HDAC5, CN, NFix, RyR1, SERCA1, Troponin T and Parvalbumin normalized by the beta-2 microglobulin gene. In each graph, the bars represent the mean values ± SEM in myopathic subjects (Statin) compared with those of untreated subjects (Ctrl) from the number of subjects indicated in brackets. ∗ Significantly different with respect to the own control ( P < 0.05 or less) by Student’s t -test.

    Journal: Frontiers in Pharmacology

    Article Title: Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study

    doi: 10.3389/fphar.2017.00500

    Figure Lengend Snippet: Real-time PCR analysis of mRNA expression level of genes involved in ClC-1 chloride channel regulation and calcium signaling in VL muscle biopsies of patients in therapy with statin showing sign of myopathy. Histograms show quantification of transcript levels for MEF2D, HDAC5, CN, NFix, RyR1, SERCA1, Troponin T and Parvalbumin normalized by the beta-2 microglobulin gene. In each graph, the bars represent the mean values ± SEM in myopathic subjects (Statin) compared with those of untreated subjects (Ctrl) from the number of subjects indicated in brackets. ∗ Significantly different with respect to the own control ( P < 0.05 or less) by Student’s t -test.

    Article Snippet: Ryr1 , Ryanodine receptor 1 (skeletal) , Hs00166991_m1.

    Techniques: Real-time Polymerase Chain Reaction, Expressing