gene exp map3k8 hs00178297 m1  (Thermo Fisher)


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    Thermo Fisher gene exp map3k8 hs00178297 m1
    Proposed model of the tumor-suppressive effects of TPL2 in the lung. TPL2 contributes to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent up-regulation of nucleophosmin. Genetic or epigenetic aberrations (e.g., loss of heterozygosity, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated carcinogenesis.
    Gene Exp Map3k8 Hs00178297 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp map3k8 hs00178297 m1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp map3k8 hs00178297 m1 - by Bioz Stars, 2020-01
    85/100 stars

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    1) Product Images from "TPL2 kinase is a suppressor of lung carcinogenesis"

    Article Title: TPL2 kinase is a suppressor of lung carcinogenesis

    Journal:

    doi: 10.1073/pnas.1215938110

    Proposed model of the tumor-suppressive effects of TPL2 in the lung. TPL2 contributes to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent up-regulation of nucleophosmin. Genetic or epigenetic aberrations (e.g., loss of heterozygosity, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated carcinogenesis.
    Figure Legend Snippet: Proposed model of the tumor-suppressive effects of TPL2 in the lung. TPL2 contributes to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent up-regulation of nucleophosmin. Genetic or epigenetic aberrations (e.g., loss of heterozygosity, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated carcinogenesis.

    Techniques Used: Over Expression

    TPL2 levels are reduced in primary human lung carcinomas and correlate with poor patient survival. ( A ) Comparison of TPL2 mRNA expression in the 100 available pairs of NSCLCs (T) and adjacent nonmalignant (N) lung tissues by qPCR. Results were normalized to the housekeeping β-actin gene and are expressed as RQ values using the IMR-90 human fibroblast cell line as calibrator. Stars and circles in the box plot indicate outliers. ( B ) Kaplan–Meier survival plot showing the cumulative survival of lung cancer patients who displayed more than twofold reduction in  TPL2  mRNA levels in T compared with N and patients with less than twofold reduction in expression levels. Patients with low  TPL2  expression have median survival of 16.2 mo (95% confidence interval = 11.8–20.6) compared with patients with less than twofold reduction, who have median survival of 28.8 mo (95% confidence interval = 18.4–39.2,  P  = 0.009; log rank test). ( C ) Inverse correlation between  TPL2  mRNA levels and Ki67 expression in cancer subset of NSCLC samples within this study ( n  = 35) for which Ki67 data were available ( r  coefficient and  P  values obtained from Spearman correlation). ( D ) TPL2 protein levels are reduced in T compared with N. TPL2 is expressed as two isoforms (noted with arrows) generated by the use of alternative translation start sites at methionines 1 and 30. ( E ) Representative immunohistochemical analysis showing a marked down-regulation of TPL2 expression in malignant cells (arrowheads) compared with nonmalignant epithelium (arrows). (Final magnification: 400×; objective lens: 40×/0.65; scale bars: 200 μm.)
    Figure Legend Snippet: TPL2 levels are reduced in primary human lung carcinomas and correlate with poor patient survival. ( A ) Comparison of TPL2 mRNA expression in the 100 available pairs of NSCLCs (T) and adjacent nonmalignant (N) lung tissues by qPCR. Results were normalized to the housekeeping β-actin gene and are expressed as RQ values using the IMR-90 human fibroblast cell line as calibrator. Stars and circles in the box plot indicate outliers. ( B ) Kaplan–Meier survival plot showing the cumulative survival of lung cancer patients who displayed more than twofold reduction in TPL2 mRNA levels in T compared with N and patients with less than twofold reduction in expression levels. Patients with low TPL2 expression have median survival of 16.2 mo (95% confidence interval = 11.8–20.6) compared with patients with less than twofold reduction, who have median survival of 28.8 mo (95% confidence interval = 18.4–39.2, P = 0.009; log rank test). ( C ) Inverse correlation between TPL2 mRNA levels and Ki67 expression in cancer subset of NSCLC samples within this study ( n = 35) for which Ki67 data were available ( r coefficient and P values obtained from Spearman correlation). ( D ) TPL2 protein levels are reduced in T compared with N. TPL2 is expressed as two isoforms (noted with arrows) generated by the use of alternative translation start sites at methionines 1 and 30. ( E ) Representative immunohistochemical analysis showing a marked down-regulation of TPL2 expression in malignant cells (arrowheads) compared with nonmalignant epithelium (arrows). (Final magnification: 400×; objective lens: 40×/0.65; scale bars: 200 μm.)

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Generated, Immunohistochemistry

    Proposed model of the tumor-suppressive effects of TPL2 in the lung. On the basis of the data presented in this paper, we propose that TPL2 suppresses lung carcinogenesis by contributing to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent NPM up-regulation. Genetic or epigenetic aberrations (LOH, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated cell transformation.
    Figure Legend Snippet: Proposed model of the tumor-suppressive effects of TPL2 in the lung. On the basis of the data presented in this paper, we propose that TPL2 suppresses lung carcinogenesis by contributing to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent NPM up-regulation. Genetic or epigenetic aberrations (LOH, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated cell transformation.

    Techniques Used: Over Expression, Transformation Assay

    Related Articles

    Amplification:

    Article Title: Analysis of the In Vitro Transcriptional Response of Human Pharyngeal Epithelial Cells to Adherent Streptococcus pneumoniae: Evidence for a Distinct Response to Encapsulated Strains
    Article Snippet: Duplicate quantitative PCR assays were performed on 1 μl of 10×-diluted cDNA using TaqMan gene expression assays (Applied Biosystems) of several target genes, encoding ADRB2 (Hs00240532_s1), CXCL2 (Hs00601975_m1), DUSP5 (Hs00244839_m1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Hs99999905_m1), IL-6 (Hs00174131_m1), IL-8 (Hs00174103_m1), MAP3K8 (Hs00178297_m1), MOAP1 (Hs00377893_g1), NF-κBIA (Hs00153283_m1), TNFAIP3 (Hs00234712_m1), TRIB1 (Hs00179769_m1), and 18S (Hs99999901_s1). .. The relative quantitation method (threshold cycle [ΔΔ CT ]) ( ) was used to evaluate the quantitative variation in gene expression between a particular experimental condition and control (uninfected) cells relative to each gene examined.

    Sequencing:

    Article Title: Constitutive Expression of the AP-1 Transcription Factors c-jun, junD, junB, and c-fos and the Marginal Zone B-Cell Transcription Factor Notch2 in Splenic Marginal Zone Lymphoma
    Article Snippet: TaqMan probes and primers for junD, junB, c-fos, and β-glucuronidase (GUS) were designed using Primer Express 1.0 (PE Applied Biosystems) and sequence data from the NCBI database. .. For detection of c-Jun, Notch2, MAP3K8, MAP2K3, MAPK1, and MAP2K6 mRNA expression we used assays-on-demand gene expression products (PE Applied Biosystems, Hs00277190_s1, Hs00225747_m1, Hs00177127_m1, Hs00178297_m1, Hs00177066_m1, and Hs00177150_m1).

    Real-time Polymerase Chain Reaction:

    Article Title: TPL2 kinase is a suppressor of lung carcinogenesis
    Article Snippet: The first and last sections underwent pathological review to ensure ≥80% tumor cell content, and 500 ng total RNA were reverse-transcribed in a 20-μL reaction using the Quantitect Kit (Qiagen) following the supplier’s protocol. .. TaqMan gene expression assays for human COT [MAP3K8; ID Hs00178297_m1, FAM (6 - Carboxyfluorescein)-labeled] and β-actin (ACTB; 4326315E) as endogenous control (VIC-labeled), mouse Cdkn1a , (Mm04205640_g1), bax (Mm00432051_m1), and GAPD (glyceraldehyde-3-phosphate dehydrogenase) assays were obtained from Applied Biosystems and used on an Applied Biosystems 7500 FAST Real-Time PCR Instrument. .. For microRNA analysis, the hsa-miR-370 (assay ID 2275) and RNU48 (assay ID 1006) were used as target and endogenous control, respectively, following the manufacturer’s protocol.

    Article Title: Constitutive Expression of the AP-1 Transcription Factors c-jun, junD, junB, and c-fos and the Marginal Zone B-Cell Transcription Factor Notch2 in Splenic Marginal Zone Lymphoma
    Article Snippet: Paragraph title: Quantitative Real-Time PCR ... For detection of c-Jun, Notch2, MAP3K8, MAP2K3, MAPK1, and MAP2K6 mRNA expression we used assays-on-demand gene expression products (PE Applied Biosystems, Hs00277190_s1, Hs00225747_m1, Hs00177127_m1, Hs00178297_m1, Hs00177066_m1, and Hs00177150_m1).

    Article Title: Analysis of the In Vitro Transcriptional Response of Human Pharyngeal Epithelial Cells to Adherent Streptococcus pneumoniae: Evidence for a Distinct Response to Encapsulated Strains
    Article Snippet: Total RNA (2 μg) was reverse transcribed using a high-capacity cDNA reverse transcription kit according to the instructions provided by the manufacturer (Applied Biosystems, Foster City, CA). .. Duplicate quantitative PCR assays were performed on 1 μl of 10×-diluted cDNA using TaqMan gene expression assays (Applied Biosystems) of several target genes, encoding ADRB2 (Hs00240532_s1), CXCL2 (Hs00601975_m1), DUSP5 (Hs00244839_m1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Hs99999905_m1), IL-6 (Hs00174131_m1), IL-8 (Hs00174103_m1), MAP3K8 (Hs00178297_m1), MOAP1 (Hs00377893_g1), NF-κBIA (Hs00153283_m1), TNFAIP3 (Hs00234712_m1), TRIB1 (Hs00179769_m1), and 18S (Hs99999901_s1). .. All assays were done in 10-μl reaction mixtures containing TaqMan universal PCR master mix, 20× TaqMan gene expression assay mix, and cDNA on an Applied Biosystems 7500 FAST real-time PCR system according to the manufacturer's instructions.

    Microarray:

    Article Title: Analysis of the In Vitro Transcriptional Response of Human Pharyngeal Epithelial Cells to Adherent Streptococcus pneumoniae: Evidence for a Distinct Response to Encapsulated Strains
    Article Snippet: Real-time quantitative PCR was used to validate selected data from the microarray experiments and to follow the expression of a subset of genes over time. .. Duplicate quantitative PCR assays were performed on 1 μl of 10×-diluted cDNA using TaqMan gene expression assays (Applied Biosystems) of several target genes, encoding ADRB2 (Hs00240532_s1), CXCL2 (Hs00601975_m1), DUSP5 (Hs00244839_m1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Hs99999905_m1), IL-6 (Hs00174131_m1), IL-8 (Hs00174103_m1), MAP3K8 (Hs00178297_m1), MOAP1 (Hs00377893_g1), NF-κBIA (Hs00153283_m1), TNFAIP3 (Hs00234712_m1), TRIB1 (Hs00179769_m1), and 18S (Hs99999901_s1).

    Quantitation Assay:

    Article Title: Analysis of the In Vitro Transcriptional Response of Human Pharyngeal Epithelial Cells to Adherent Streptococcus pneumoniae: Evidence for a Distinct Response to Encapsulated Strains
    Article Snippet: Duplicate quantitative PCR assays were performed on 1 μl of 10×-diluted cDNA using TaqMan gene expression assays (Applied Biosystems) of several target genes, encoding ADRB2 (Hs00240532_s1), CXCL2 (Hs00601975_m1), DUSP5 (Hs00244839_m1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Hs99999905_m1), IL-6 (Hs00174131_m1), IL-8 (Hs00174103_m1), MAP3K8 (Hs00178297_m1), MOAP1 (Hs00377893_g1), NF-κBIA (Hs00153283_m1), TNFAIP3 (Hs00234712_m1), TRIB1 (Hs00179769_m1), and 18S (Hs99999901_s1). .. All assays were done in 10-μl reaction mixtures containing TaqMan universal PCR master mix, 20× TaqMan gene expression assay mix, and cDNA on an Applied Biosystems 7500 FAST real-time PCR system according to the manufacturer's instructions.

    Expressing:

    Article Title: TPL2 kinase is a suppressor of lung carcinogenesis
    Article Snippet: The first and last sections underwent pathological review to ensure ≥80% tumor cell content, and 500 ng total RNA were reverse-transcribed in a 20-μL reaction using the Quantitect Kit (Qiagen) following the supplier’s protocol. .. TaqMan gene expression assays for human COT [MAP3K8; ID Hs00178297_m1, FAM (6 - Carboxyfluorescein)-labeled] and β-actin (ACTB; 4326315E) as endogenous control (VIC-labeled), mouse Cdkn1a , (Mm04205640_g1), bax (Mm00432051_m1), and GAPD (glyceraldehyde-3-phosphate dehydrogenase) assays were obtained from Applied Biosystems and used on an Applied Biosystems 7500 FAST Real-Time PCR Instrument. .. For microRNA analysis, the hsa-miR-370 (assay ID 2275) and RNU48 (assay ID 1006) were used as target and endogenous control, respectively, following the manufacturer’s protocol.

    Article Title: Constitutive Expression of the AP-1 Transcription Factors c-jun, junD, junB, and c-fos and the Marginal Zone B-Cell Transcription Factor Notch2 in Splenic Marginal Zone Lymphoma
    Article Snippet: The following primer and probes were used: c-fos: forward 5′-CAGCGAGCAACTGAGAAGCC, reverse 5′-CGCTGTGAAGCA-GAGCTGG, FAM5′-CAGCGAACGAGCAGTGACCGTGC-TAMRA; junD: forward 5′-TGACGCTGAGCCTGA GTGAG, reverse 5′TCGGGAGAGGCGAGCA, FAM5′-TGAGCGCTGCCGCCACCT, junB: forward 5′GTCACCGAGGAGCAGGAGG, reverse 5′-TCTTGTGCAGATCGTCCAGG, FAM5′-TTCGCCGACGGCTTTGTCAAAG-TAMRA, GUS: forward 5′-GAAAATATGTGGTT GGAGAGCTCATT, reverse 5′-CCGAGTGAAGATCCCCTTTTTA, FAM5′-CCAGCACTCTCGTCGGTGACTGTTCA-TAMRA. .. For detection of c-Jun, Notch2, MAP3K8, MAP2K3, MAPK1, and MAP2K6 mRNA expression we used assays-on-demand gene expression products (PE Applied Biosystems, Hs00277190_s1, Hs00225747_m1, Hs00177127_m1, Hs00178297_m1, Hs00177066_m1, and Hs00177150_m1). .. For all PCR reactions, 0.5 μl of cDNA, 300 nmol/L of specific primers, 100 nmol/L probe, and 12.5 μl of the Universal TaqMan 2X PCR mastermix (PE Applied Biosystems) were mixed to a final volume of 25 μl.

    Article Title: The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development
    Article Snippet: The housekeeping gene used for input normalization of all the qRT-PCR data is β-actin. .. Taqman Gene Expression Assays used: Kmt2d (Mm02600438_m1), β- actin (#4352663), Socs3 (Mm00545913), Dusp1 (Mm00457274), Tnfaip3 (Mm00437121), Arid1a (Mm00473838), Fos (Mm00487425), Ikbkb (Mm01222247), Tnfrsf14 (Mm00619239), KMT2D (Hs00231606), SOCS3 (Hs02330328), TNFRSF14 (Hs00998604), TNFAIP3 (Hs00234713), ARID1A (Hs00195664), DUSP1 (Hs00610256), TRAF3 (Hs00936781), NR4A1 (Hs00374226), IKBKB (Hs00233287), DNMT3A (Hs01027166), ASXL1 (Hs00392415), ARID3B (Hs00356736), MAP3K8 (Hs00178297) and ACTB (#4352667). .. Sample sizes for comparisons between cell types or between mouse genotypes followed Mead's recommendations .

    Article Title: Analysis of the In Vitro Transcriptional Response of Human Pharyngeal Epithelial Cells to Adherent Streptococcus pneumoniae: Evidence for a Distinct Response to Encapsulated Strains
    Article Snippet: Total RNA (2 μg) was reverse transcribed using a high-capacity cDNA reverse transcription kit according to the instructions provided by the manufacturer (Applied Biosystems, Foster City, CA). .. Duplicate quantitative PCR assays were performed on 1 μl of 10×-diluted cDNA using TaqMan gene expression assays (Applied Biosystems) of several target genes, encoding ADRB2 (Hs00240532_s1), CXCL2 (Hs00601975_m1), DUSP5 (Hs00244839_m1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Hs99999905_m1), IL-6 (Hs00174131_m1), IL-8 (Hs00174103_m1), MAP3K8 (Hs00178297_m1), MOAP1 (Hs00377893_g1), NF-κBIA (Hs00153283_m1), TNFAIP3 (Hs00234712_m1), TRIB1 (Hs00179769_m1), and 18S (Hs99999901_s1). .. All assays were done in 10-μl reaction mixtures containing TaqMan universal PCR master mix, 20× TaqMan gene expression assay mix, and cDNA on an Applied Biosystems 7500 FAST real-time PCR system according to the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Constitutive Expression of the AP-1 Transcription Factors c-jun, junD, junB, and c-fos and the Marginal Zone B-Cell Transcription Factor Notch2 in Splenic Marginal Zone Lymphoma
    Article Snippet: For detection of c-Jun, Notch2, MAP3K8, MAP2K3, MAPK1, and MAP2K6 mRNA expression we used assays-on-demand gene expression products (PE Applied Biosystems, Hs00277190_s1, Hs00225747_m1, Hs00177127_m1, Hs00178297_m1, Hs00177066_m1, and Hs00177150_m1). .. For detection of c-Jun, Notch2, MAP3K8, MAP2K3, MAPK1, and MAP2K6 mRNA expression we used assays-on-demand gene expression products (PE Applied Biosystems, Hs00277190_s1, Hs00225747_m1, Hs00177127_m1, Hs00178297_m1, Hs00177066_m1, and Hs00177150_m1).

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    Thermo Fisher gene exp map3k8 hs00178297 m1
    Proposed model of the tumor-suppressive effects of TPL2 in the lung. TPL2 contributes to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent up-regulation of nucleophosmin. Genetic or epigenetic aberrations (e.g., loss of heterozygosity, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated carcinogenesis.
    Gene Exp Map3k8 Hs00178297 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp map3k8 hs00178297 m1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp map3k8 hs00178297 m1 - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    84
    Thermo Fisher gene exp zc3h12a hs00962356 m1
    Suppression of luciferase expression by the 5′-UTR of MCPIP1 mRNA. A , scheme of luciferase ( Luc )-MCPIP1 reporter mRNAs investigated in this study. They contain insertions of the 5′-UTR and 3′-UTR of MCPIP1, the 5′-UTR alone,
    Gene Exp Zc3h12a Hs00962356 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp zc3h12a hs00962356 m1/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp zc3h12a hs00962356 m1 - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    Image Search Results


    Proposed model of the tumor-suppressive effects of TPL2 in the lung. TPL2 contributes to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent up-regulation of nucleophosmin. Genetic or epigenetic aberrations (e.g., loss of heterozygosity, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated carcinogenesis.

    Journal:

    Article Title: TPL2 kinase is a suppressor of lung carcinogenesis

    doi: 10.1073/pnas.1215938110

    Figure Lengend Snippet: Proposed model of the tumor-suppressive effects of TPL2 in the lung. TPL2 contributes to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent up-regulation of nucleophosmin. Genetic or epigenetic aberrations (e.g., loss of heterozygosity, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated carcinogenesis.

    Article Snippet: TaqMan gene expression assays for human COT [MAP3K8; ID Hs00178297_m1, FAM (6 - Carboxyfluorescein)-labeled] and β-actin (ACTB; 4326315E) as endogenous control (VIC-labeled), mouse Cdkn1a , (Mm04205640_g1), bax (Mm00432051_m1), and GAPD (glyceraldehyde-3-phosphate dehydrogenase) assays were obtained from Applied Biosystems and used on an Applied Biosystems 7500 FAST Real-Time PCR Instrument.

    Techniques: Over Expression

    TPL2 levels are reduced in primary human lung carcinomas and correlate with poor patient survival. ( A ) Comparison of TPL2 mRNA expression in the 100 available pairs of NSCLCs (T) and adjacent nonmalignant (N) lung tissues by qPCR. Results were normalized to the housekeeping β-actin gene and are expressed as RQ values using the IMR-90 human fibroblast cell line as calibrator. Stars and circles in the box plot indicate outliers. ( B ) Kaplan–Meier survival plot showing the cumulative survival of lung cancer patients who displayed more than twofold reduction in  TPL2  mRNA levels in T compared with N and patients with less than twofold reduction in expression levels. Patients with low  TPL2  expression have median survival of 16.2 mo (95% confidence interval = 11.8–20.6) compared with patients with less than twofold reduction, who have median survival of 28.8 mo (95% confidence interval = 18.4–39.2,  P  = 0.009; log rank test). ( C ) Inverse correlation between  TPL2  mRNA levels and Ki67 expression in cancer subset of NSCLC samples within this study ( n  = 35) for which Ki67 data were available ( r  coefficient and  P  values obtained from Spearman correlation). ( D ) TPL2 protein levels are reduced in T compared with N. TPL2 is expressed as two isoforms (noted with arrows) generated by the use of alternative translation start sites at methionines 1 and 30. ( E ) Representative immunohistochemical analysis showing a marked down-regulation of TPL2 expression in malignant cells (arrowheads) compared with nonmalignant epithelium (arrows). (Final magnification: 400×; objective lens: 40×/0.65; scale bars: 200 μm.)

    Journal:

    Article Title: TPL2 kinase is a suppressor of lung carcinogenesis

    doi: 10.1073/pnas.1215938110

    Figure Lengend Snippet: TPL2 levels are reduced in primary human lung carcinomas and correlate with poor patient survival. ( A ) Comparison of TPL2 mRNA expression in the 100 available pairs of NSCLCs (T) and adjacent nonmalignant (N) lung tissues by qPCR. Results were normalized to the housekeeping β-actin gene and are expressed as RQ values using the IMR-90 human fibroblast cell line as calibrator. Stars and circles in the box plot indicate outliers. ( B ) Kaplan–Meier survival plot showing the cumulative survival of lung cancer patients who displayed more than twofold reduction in TPL2 mRNA levels in T compared with N and patients with less than twofold reduction in expression levels. Patients with low TPL2 expression have median survival of 16.2 mo (95% confidence interval = 11.8–20.6) compared with patients with less than twofold reduction, who have median survival of 28.8 mo (95% confidence interval = 18.4–39.2, P = 0.009; log rank test). ( C ) Inverse correlation between TPL2 mRNA levels and Ki67 expression in cancer subset of NSCLC samples within this study ( n = 35) for which Ki67 data were available ( r coefficient and P values obtained from Spearman correlation). ( D ) TPL2 protein levels are reduced in T compared with N. TPL2 is expressed as two isoforms (noted with arrows) generated by the use of alternative translation start sites at methionines 1 and 30. ( E ) Representative immunohistochemical analysis showing a marked down-regulation of TPL2 expression in malignant cells (arrowheads) compared with nonmalignant epithelium (arrows). (Final magnification: 400×; objective lens: 40×/0.65; scale bars: 200 μm.)

    Article Snippet: TaqMan gene expression assays for human COT [MAP3K8; ID Hs00178297_m1, FAM (6 - Carboxyfluorescein)-labeled] and β-actin (ACTB; 4326315E) as endogenous control (VIC-labeled), mouse Cdkn1a , (Mm04205640_g1), bax (Mm00432051_m1), and GAPD (glyceraldehyde-3-phosphate dehydrogenase) assays were obtained from Applied Biosystems and used on an Applied Biosystems 7500 FAST Real-Time PCR Instrument.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Generated, Immunohistochemistry

    Proposed model of the tumor-suppressive effects of TPL2 in the lung. On the basis of the data presented in this paper, we propose that TPL2 suppresses lung carcinogenesis by contributing to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent NPM up-regulation. Genetic or epigenetic aberrations (LOH, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated cell transformation.

    Journal:

    Article Title: TPL2 kinase is a suppressor of lung carcinogenesis

    doi: 10.1073/pnas.1215938110

    Figure Lengend Snippet: Proposed model of the tumor-suppressive effects of TPL2 in the lung. On the basis of the data presented in this paper, we propose that TPL2 suppresses lung carcinogenesis by contributing to p53 response to DNA damage caused by genotoxic agents or oncogenic stress through JNK-dependent NPM up-regulation. Genetic or epigenetic aberrations (LOH, overexpression of miR-370, or oncogenic RAS signaling) may independently induce TPL2 down-regulation, leading to diminished JNK and p53 responses, reduced cell death, and accelerated cell transformation.

    Article Snippet: TaqMan gene expression assays for human COT [MAP3K8; ID Hs00178297_m1, FAM (6 - Carboxyfluorescein)-labeled] and β-actin (ACTB; 4326315E) as endogenous control (VIC-labeled), mouse Cdkn1a , (Mm04205640_g1), bax (Mm00432051_m1), and GAPD (glyceraldehyde-3-phosphate dehydrogenase) assays were obtained from Applied Biosystems and used on an Applied Biosystems 7500 FAST Real-Time PCR Instrument.

    Techniques: Over Expression, Transformation Assay

    Suppression of luciferase expression by the 5′-UTR of MCPIP1 mRNA. A , scheme of luciferase ( Luc )-MCPIP1 reporter mRNAs investigated in this study. They contain insertions of the 5′-UTR and 3′-UTR of MCPIP1, the 5′-UTR alone,

    Journal:

    Article Title:

    doi: 10.1074/jbc.M113.452649

    Figure Lengend Snippet: Suppression of luciferase expression by the 5′-UTR of MCPIP1 mRNA. A , scheme of luciferase ( Luc )-MCPIP1 reporter mRNAs investigated in this study. They contain insertions of the 5′-UTR and 3′-UTR of MCPIP1, the 5′-UTR alone,

    Article Snippet: Reverse transcription and quantitative PCR (RT-qPCR) were carried out as described , using TaqMan assays (Applied Biosystems, assay IDs Hs00962356_m1 and Mm01182027_m1 for human and murine MCPIP1 mRNA, respectively, Hs00230071_m1 and Mm00600522_m1 for human and murine IκBζ mRNA, respectively, Hs00174131_m1 for IL-6 mRNA, and Hs99999905_m1 for GAPDH mRNA, Hs00178297_m1 for MAP3K8 mRNA, and custom-made assays for rabbit β-globin and firefly luciferase mRNAs). mRNA of the GFP-MCPIP1 fusion protein was quantified by SYBR Green-based detection (GFP-specific primers: sense, 5′-tgcagtgcttcagccgctac; antisense, 5′-tcgccctcgaacttcacctc).

    Techniques: Luciferase, Expressing

    Redistribution of MCPIP1 mRNA to polysomes in synovial fibroblasts and macrophages. A , synovial fibroblasts from a patient with osteoarthritis ( OASF ) and from a patient with rheumatoid arthritis ( RASF ) were incubated with IL-1α (2 ng/ml) for 1

    Journal:

    Article Title:

    doi: 10.1074/jbc.M113.452649

    Figure Lengend Snippet: Redistribution of MCPIP1 mRNA to polysomes in synovial fibroblasts and macrophages. A , synovial fibroblasts from a patient with osteoarthritis ( OASF ) and from a patient with rheumatoid arthritis ( RASF ) were incubated with IL-1α (2 ng/ml) for 1

    Article Snippet: Reverse transcription and quantitative PCR (RT-qPCR) were carried out as described , using TaqMan assays (Applied Biosystems, assay IDs Hs00962356_m1 and Mm01182027_m1 for human and murine MCPIP1 mRNA, respectively, Hs00230071_m1 and Mm00600522_m1 for human and murine IκBζ mRNA, respectively, Hs00174131_m1 for IL-6 mRNA, and Hs99999905_m1 for GAPDH mRNA, Hs00178297_m1 for MAP3K8 mRNA, and custom-made assays for rabbit β-globin and firefly luciferase mRNAs). mRNA of the GFP-MCPIP1 fusion protein was quantified by SYBR Green-based detection (GFP-specific primers: sense, 5′-tgcagtgcttcagccgctac; antisense, 5′-tcgccctcgaacttcacctc).

    Techniques: Incubation

    Suppression of GFP-MCPIP1 expression by the 3′-UTR of MCPIP1 mRNA. A , HeLa cells were transfected with plasmids containing sequences for a GFP-MCPIP1 fusion protein followed by the MCPIP1 3′-UTR ( GFP-Mcds-M3′ ) or not ( GFP-Mcds

    Journal:

    Article Title:

    doi: 10.1074/jbc.M113.452649

    Figure Lengend Snippet: Suppression of GFP-MCPIP1 expression by the 3′-UTR of MCPIP1 mRNA. A , HeLa cells were transfected with plasmids containing sequences for a GFP-MCPIP1 fusion protein followed by the MCPIP1 3′-UTR ( GFP-Mcds-M3′ ) or not ( GFP-Mcds

    Article Snippet: Reverse transcription and quantitative PCR (RT-qPCR) were carried out as described , using TaqMan assays (Applied Biosystems, assay IDs Hs00962356_m1 and Mm01182027_m1 for human and murine MCPIP1 mRNA, respectively, Hs00230071_m1 and Mm00600522_m1 for human and murine IκBζ mRNA, respectively, Hs00174131_m1 for IL-6 mRNA, and Hs99999905_m1 for GAPDH mRNA, Hs00178297_m1 for MAP3K8 mRNA, and custom-made assays for rabbit β-globin and firefly luciferase mRNAs). mRNA of the GFP-MCPIP1 fusion protein was quantified by SYBR Green-based detection (GFP-specific primers: sense, 5′-tgcagtgcttcagccgctac; antisense, 5′-tcgccctcgaacttcacctc).

    Techniques: Expressing, Transfection

    Effect of different cytokines on the degradation of MCPIP1 and IL-6 mRNAs. A , HeLa cells were incubated without ( con ) or with the indicated cytokines for 15 min followed by the addition of actinomycin D (5 μg/ml). Total RNA was isolated at the

    Journal:

    Article Title:

    doi: 10.1074/jbc.M113.452649

    Figure Lengend Snippet: Effect of different cytokines on the degradation of MCPIP1 and IL-6 mRNAs. A , HeLa cells were incubated without ( con ) or with the indicated cytokines for 15 min followed by the addition of actinomycin D (5 μg/ml). Total RNA was isolated at the

    Article Snippet: Reverse transcription and quantitative PCR (RT-qPCR) were carried out as described , using TaqMan assays (Applied Biosystems, assay IDs Hs00962356_m1 and Mm01182027_m1 for human and murine MCPIP1 mRNA, respectively, Hs00230071_m1 and Mm00600522_m1 for human and murine IκBζ mRNA, respectively, Hs00174131_m1 for IL-6 mRNA, and Hs99999905_m1 for GAPDH mRNA, Hs00178297_m1 for MAP3K8 mRNA, and custom-made assays for rabbit β-globin and firefly luciferase mRNAs). mRNA of the GFP-MCPIP1 fusion protein was quantified by SYBR Green-based detection (GFP-specific primers: sense, 5′-tgcagtgcttcagccgctac; antisense, 5′-tcgccctcgaacttcacctc).

    Techniques: Incubation, Isolation

    MCPIP1 3′-UTR-mediated suppression is counteracted by IL-1 and IL-17 and dependent on a putative stem-loop-forming sequence. A , cells transfected with plasmids for firefly luciferase ( Luc ) without or with the MCPIP1 UTRs as indicated (see scheme

    Journal:

    Article Title:

    doi: 10.1074/jbc.M113.452649

    Figure Lengend Snippet: MCPIP1 3′-UTR-mediated suppression is counteracted by IL-1 and IL-17 and dependent on a putative stem-loop-forming sequence. A , cells transfected with plasmids for firefly luciferase ( Luc ) without or with the MCPIP1 UTRs as indicated (see scheme

    Article Snippet: Reverse transcription and quantitative PCR (RT-qPCR) were carried out as described , using TaqMan assays (Applied Biosystems, assay IDs Hs00962356_m1 and Mm01182027_m1 for human and murine MCPIP1 mRNA, respectively, Hs00230071_m1 and Mm00600522_m1 for human and murine IκBζ mRNA, respectively, Hs00174131_m1 for IL-6 mRNA, and Hs99999905_m1 for GAPDH mRNA, Hs00178297_m1 for MAP3K8 mRNA, and custom-made assays for rabbit β-globin and firefly luciferase mRNAs). mRNA of the GFP-MCPIP1 fusion protein was quantified by SYBR Green-based detection (GFP-specific primers: sense, 5′-tgcagtgcttcagccgctac; antisense, 5′-tcgccctcgaacttcacctc).

    Techniques: Sequencing, Transfection, Luciferase