gene exp fbxo7 mm00462692 m1  (Thermo Fisher)


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    Thermo Fisher gene exp fbxo7 mm00462692 m1
    <t>Fbxo7</t> tm1a mice have reduced circulating T cells with an altered phenotype and impaired T cell development. (A) Circulating T cell percentage in peripheral blood, (B) blood T cell effector percentage. (C) T cell percentage in the spleen and (D) splenic T cell effector percentage. (E) total thymus cell number and (F) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. All mice were female and 8 weeks old. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction except panel E which is from unpaired two-tailed students t tests with Welch’s correction. All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.
    Gene Exp Fbxo7 Mm00462692 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp fbxo7 mm00462692 m1/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp fbxo7 mm00462692 m1 - by Bioz Stars, 2020-01
    82/100 stars

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    1) Product Images from "FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele"

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0212481

    Fbxo7 tm1a mice have reduced circulating T cells with an altered phenotype and impaired T cell development. (A) Circulating T cell percentage in peripheral blood, (B) blood T cell effector percentage. (C) T cell percentage in the spleen and (D) splenic T cell effector percentage. (E) total thymus cell number and (F) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. All mice were female and 8 weeks old. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction except panel E which is from unpaired two-tailed students t tests with Welch’s correction. All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.
    Figure Legend Snippet: Fbxo7 tm1a mice have reduced circulating T cells with an altered phenotype and impaired T cell development. (A) Circulating T cell percentage in peripheral blood, (B) blood T cell effector percentage. (C) T cell percentage in the spleen and (D) splenic T cell effector percentage. (E) total thymus cell number and (F) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. All mice were female and 8 weeks old. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction except panel E which is from unpaired two-tailed students t tests with Welch’s correction. All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.

    Techniques Used: Mouse Assay, Two Tailed Test

    Fbxo7 targeted mice present with anaemia and extramedullary haematopoiesis. (A) Red blood cell count (RBC); (B) haemoglobin; (C) haematocrit; (D) mean corpuscular haemoglobin concentration (MCHC); (E) mean corpuscular volume (MCV); (F) red blood cell distribution width (RDW); (G) spleen weight; (H) characterisation of splenic erythrocyte development; and (I) circulating reticulocyte; from 15–17 week old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. P values calculated from unpaired two-tailed students t tests with Welch’s correction (A-G, I), or unpaired two-tailed students t tests with Holm-Sidak multiple testing correction (H). The data are representative of at least 3 independent experiments and each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation or standard error of the mean for panel H.
    Figure Legend Snippet: Fbxo7 targeted mice present with anaemia and extramedullary haematopoiesis. (A) Red blood cell count (RBC); (B) haemoglobin; (C) haematocrit; (D) mean corpuscular haemoglobin concentration (MCHC); (E) mean corpuscular volume (MCV); (F) red blood cell distribution width (RDW); (G) spleen weight; (H) characterisation of splenic erythrocyte development; and (I) circulating reticulocyte; from 15–17 week old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. P values calculated from unpaired two-tailed students t tests with Welch’s correction (A-G, I), or unpaired two-tailed students t tests with Holm-Sidak multiple testing correction (H). The data are representative of at least 3 independent experiments and each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation or standard error of the mean for panel H.

    Techniques Used: Mouse Assay, Cell Counting, Concentration Assay, Two Tailed Test, Standard Deviation

    Increased morbidity after infection of Fbxo7 tm1a mice with Salmonella typhimurium. (A) Survival curve of 4 female and 4 male Fbxo7 +/+ mice and 3 female and 4 male Fbxo7 tm1a/tm1a mice. (B) body weight changes during the course of infection from mice shown in panel A. (C) spleen weights at day 6 post Salmonella infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (D) spleen and liver Salmonella counts at day 6 post infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (E) histopathological image of liver showing a typical necro-inflammatory focus with an oval nodule consisting of eosinophilic necrotic hepatocytes surrounded by a rim of inflammatory cells that are predominantly neutrophil leucocytes, from a male Fbxo7 tm1a/tm1a mouse at day 6. (F) body weight changes during course of infection for male mice deficient for Fbxo7 in T cells ( Fbxo7 tm1c/tm1c ;CD4-Cre + 8 mice) and littermate controls ( Fbxo7 tm1c/tm1c ;CD4-Cre— 5 mice). (G) spleen and liver Salmonella counts at day 14 post infection from irradiated female mice reconstituted with Fbxo7 +/+ (10 mice) or Fbxo7 tm1a/tm1a bone marrow (9 mice). (H-L) plasma clinical chemistry parameters at day 6 post infection from male mice (5 mice/genotype) for plasma levels of amylase, lipase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and ferritin (P values calculated from unpaired two-tailed students t tests with Welch’s correction). For panels C, D and G-L symbols represent individual mice with the line at the mean and error bars representing the standard error of the mean (D and G) or standard deviation (C, H-L). For panel F symbols represent the mean with error bars representing the standard error of the mean.
    Figure Legend Snippet: Increased morbidity after infection of Fbxo7 tm1a mice with Salmonella typhimurium. (A) Survival curve of 4 female and 4 male Fbxo7 +/+ mice and 3 female and 4 male Fbxo7 tm1a/tm1a mice. (B) body weight changes during the course of infection from mice shown in panel A. (C) spleen weights at day 6 post Salmonella infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (D) spleen and liver Salmonella counts at day 6 post infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (E) histopathological image of liver showing a typical necro-inflammatory focus with an oval nodule consisting of eosinophilic necrotic hepatocytes surrounded by a rim of inflammatory cells that are predominantly neutrophil leucocytes, from a male Fbxo7 tm1a/tm1a mouse at day 6. (F) body weight changes during course of infection for male mice deficient for Fbxo7 in T cells ( Fbxo7 tm1c/tm1c ;CD4-Cre + 8 mice) and littermate controls ( Fbxo7 tm1c/tm1c ;CD4-Cre— 5 mice). (G) spleen and liver Salmonella counts at day 14 post infection from irradiated female mice reconstituted with Fbxo7 +/+ (10 mice) or Fbxo7 tm1a/tm1a bone marrow (9 mice). (H-L) plasma clinical chemistry parameters at day 6 post infection from male mice (5 mice/genotype) for plasma levels of amylase, lipase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and ferritin (P values calculated from unpaired two-tailed students t tests with Welch’s correction). For panels C, D and G-L symbols represent individual mice with the line at the mean and error bars representing the standard error of the mean (D and G) or standard deviation (C, H-L). For panel F symbols represent the mean with error bars representing the standard error of the mean.

    Techniques Used: Infection, Mouse Assay, Irradiation, AST Assay, Two Tailed Test, Standard Deviation

    Fbxo7 has a T cell intrinsic role in controlling T cell number in the periphery but not phenotype. (A) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. (B) T cell percentage in the spleen and (C) splenic T cell effector percentage. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction. All data representative of three independent experiments (spleen 16–20 week old female mice, thymus 12–14 week old male mice), each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.
    Figure Legend Snippet: Fbxo7 has a T cell intrinsic role in controlling T cell number in the periphery but not phenotype. (A) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. (B) T cell percentage in the spleen and (C) splenic T cell effector percentage. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction. All data representative of three independent experiments (spleen 16–20 week old female mice, thymus 12–14 week old male mice), each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.

    Techniques Used: Two Tailed Test, Mouse Assay

    Defects in the testis and epididymis of Fbxo7 tm1a/tm1a mice. (A) Image of epididymal spermatozoa smear stained with hemacolor from 12-14-week-old mice. (B) H E stained section of epididymis from 20-week-old mice. (C) testis weight from 18-19-week-old mice. (D) semithin toloudine blue stained section image of testis from 8-week-old mice. Images are representative of at least two mice per genotype, P value from an unpaired two-tailed students t test with Welch’s correction with each symbol representing a testis, line is at the mean and error bars represent standard deviation.
    Figure Legend Snippet: Defects in the testis and epididymis of Fbxo7 tm1a/tm1a mice. (A) Image of epididymal spermatozoa smear stained with hemacolor from 12-14-week-old mice. (B) H E stained section of epididymis from 20-week-old mice. (C) testis weight from 18-19-week-old mice. (D) semithin toloudine blue stained section image of testis from 8-week-old mice. Images are representative of at least two mice per genotype, P value from an unpaired two-tailed students t test with Welch’s correction with each symbol representing a testis, line is at the mean and error bars represent standard deviation.

    Techniques Used: Mouse Assay, Staining, Two Tailed Test, Standard Deviation

    Anaemia in Fbxo7 targeted mice is regenerative with a shorter erythrocyte half-life that is haematopoietic intrinsic. (A) plasma bilirubin and (B) plasma iron from 15-17-week-old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. (C) In vivo half-life of Fbxo7 +/+ and Fbxo7 tm1a/tm1a erythrocytes, the P value indicated is for each time point. D-H) characterisation of erythrocyte indices from Fbxo7 +/+ and Fbxo7 tm1a/tm1a bone marrow chimeras 10 weeks after reconstitution (female recipient mice). All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation, except for (C) where n = 6 for Fbxo7 +/+ and Fbxo7 tm1a/tm1a with mean ± standard error of the mean. P values from an unpaired two-tailed students t test with Welch’s correction (A, B, D-H) or C two-way repeated measures ANOVA with Sidak post-hoc test for individual time points.
    Figure Legend Snippet: Anaemia in Fbxo7 targeted mice is regenerative with a shorter erythrocyte half-life that is haematopoietic intrinsic. (A) plasma bilirubin and (B) plasma iron from 15-17-week-old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. (C) In vivo half-life of Fbxo7 +/+ and Fbxo7 tm1a/tm1a erythrocytes, the P value indicated is for each time point. D-H) characterisation of erythrocyte indices from Fbxo7 +/+ and Fbxo7 tm1a/tm1a bone marrow chimeras 10 weeks after reconstitution (female recipient mice). All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation, except for (C) where n = 6 for Fbxo7 +/+ and Fbxo7 tm1a/tm1a with mean ± standard error of the mean. P values from an unpaired two-tailed students t test with Welch’s correction (A, B, D-H) or C two-way repeated measures ANOVA with Sidak post-hoc test for individual time points.

    Techniques Used: Mouse Assay, In Vivo, Standard Deviation, Two Tailed Test

    Related Articles

    Expressing:

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele
    Article Snippet: .. Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples). .. Template RNA was added in duplex reactions in triplicate with endogenous control B2m VIC primer limited probe (Mm00437762_m1), using the EXPRESS One-Step Superscript qRT-PCR Kit (Thermo Scientific) and an Applied Biosystems Viia7 analyser.

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    Thermo Fisher gene exp fbxo7 mm00462692 m1
    <t>Fbxo7</t> tm1a mice have reduced circulating T cells with an altered phenotype and impaired T cell development. (A) Circulating T cell percentage in peripheral blood, (B) blood T cell effector percentage. (C) T cell percentage in the spleen and (D) splenic T cell effector percentage. (E) total thymus cell number and (F) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. All mice were female and 8 weeks old. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction except panel E which is from unpaired two-tailed students t tests with Welch’s correction. All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.
    Gene Exp Fbxo7 Mm00462692 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp fbxo7 mm00462692 m1/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp fbxo7 mm00462692 m1 - by Bioz Stars, 2020-01
    82/100 stars
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    Fbxo7 tm1a mice have reduced circulating T cells with an altered phenotype and impaired T cell development. (A) Circulating T cell percentage in peripheral blood, (B) blood T cell effector percentage. (C) T cell percentage in the spleen and (D) splenic T cell effector percentage. (E) total thymus cell number and (F) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. All mice were female and 8 weeks old. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction except panel E which is from unpaired two-tailed students t tests with Welch’s correction. All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.

    Journal: PLoS ONE

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    doi: 10.1371/journal.pone.0212481

    Figure Lengend Snippet: Fbxo7 tm1a mice have reduced circulating T cells with an altered phenotype and impaired T cell development. (A) Circulating T cell percentage in peripheral blood, (B) blood T cell effector percentage. (C) T cell percentage in the spleen and (D) splenic T cell effector percentage. (E) total thymus cell number and (F) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. All mice were female and 8 weeks old. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction except panel E which is from unpaired two-tailed students t tests with Welch’s correction. All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.

    Article Snippet: Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples).

    Techniques: Mouse Assay, Two Tailed Test

    Fbxo7 targeted mice present with anaemia and extramedullary haematopoiesis. (A) Red blood cell count (RBC); (B) haemoglobin; (C) haematocrit; (D) mean corpuscular haemoglobin concentration (MCHC); (E) mean corpuscular volume (MCV); (F) red blood cell distribution width (RDW); (G) spleen weight; (H) characterisation of splenic erythrocyte development; and (I) circulating reticulocyte; from 15–17 week old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. P values calculated from unpaired two-tailed students t tests with Welch’s correction (A-G, I), or unpaired two-tailed students t tests with Holm-Sidak multiple testing correction (H). The data are representative of at least 3 independent experiments and each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation or standard error of the mean for panel H.

    Journal: PLoS ONE

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    doi: 10.1371/journal.pone.0212481

    Figure Lengend Snippet: Fbxo7 targeted mice present with anaemia and extramedullary haematopoiesis. (A) Red blood cell count (RBC); (B) haemoglobin; (C) haematocrit; (D) mean corpuscular haemoglobin concentration (MCHC); (E) mean corpuscular volume (MCV); (F) red blood cell distribution width (RDW); (G) spleen weight; (H) characterisation of splenic erythrocyte development; and (I) circulating reticulocyte; from 15–17 week old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. P values calculated from unpaired two-tailed students t tests with Welch’s correction (A-G, I), or unpaired two-tailed students t tests with Holm-Sidak multiple testing correction (H). The data are representative of at least 3 independent experiments and each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation or standard error of the mean for panel H.

    Article Snippet: Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples).

    Techniques: Mouse Assay, Cell Counting, Concentration Assay, Two Tailed Test, Standard Deviation

    Increased morbidity after infection of Fbxo7 tm1a mice with Salmonella typhimurium. (A) Survival curve of 4 female and 4 male Fbxo7 +/+ mice and 3 female and 4 male Fbxo7 tm1a/tm1a mice. (B) body weight changes during the course of infection from mice shown in panel A. (C) spleen weights at day 6 post Salmonella infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (D) spleen and liver Salmonella counts at day 6 post infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (E) histopathological image of liver showing a typical necro-inflammatory focus with an oval nodule consisting of eosinophilic necrotic hepatocytes surrounded by a rim of inflammatory cells that are predominantly neutrophil leucocytes, from a male Fbxo7 tm1a/tm1a mouse at day 6. (F) body weight changes during course of infection for male mice deficient for Fbxo7 in T cells ( Fbxo7 tm1c/tm1c ;CD4-Cre + 8 mice) and littermate controls ( Fbxo7 tm1c/tm1c ;CD4-Cre— 5 mice). (G) spleen and liver Salmonella counts at day 14 post infection from irradiated female mice reconstituted with Fbxo7 +/+ (10 mice) or Fbxo7 tm1a/tm1a bone marrow (9 mice). (H-L) plasma clinical chemistry parameters at day 6 post infection from male mice (5 mice/genotype) for plasma levels of amylase, lipase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and ferritin (P values calculated from unpaired two-tailed students t tests with Welch’s correction). For panels C, D and G-L symbols represent individual mice with the line at the mean and error bars representing the standard error of the mean (D and G) or standard deviation (C, H-L). For panel F symbols represent the mean with error bars representing the standard error of the mean.

    Journal: PLoS ONE

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    doi: 10.1371/journal.pone.0212481

    Figure Lengend Snippet: Increased morbidity after infection of Fbxo7 tm1a mice with Salmonella typhimurium. (A) Survival curve of 4 female and 4 male Fbxo7 +/+ mice and 3 female and 4 male Fbxo7 tm1a/tm1a mice. (B) body weight changes during the course of infection from mice shown in panel A. (C) spleen weights at day 6 post Salmonella infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (D) spleen and liver Salmonella counts at day 6 post infection of male mice, with 3 Fbxo7 +/+ and 4 Fbxo7 tm1a/tm1a mice. (E) histopathological image of liver showing a typical necro-inflammatory focus with an oval nodule consisting of eosinophilic necrotic hepatocytes surrounded by a rim of inflammatory cells that are predominantly neutrophil leucocytes, from a male Fbxo7 tm1a/tm1a mouse at day 6. (F) body weight changes during course of infection for male mice deficient for Fbxo7 in T cells ( Fbxo7 tm1c/tm1c ;CD4-Cre + 8 mice) and littermate controls ( Fbxo7 tm1c/tm1c ;CD4-Cre— 5 mice). (G) spleen and liver Salmonella counts at day 14 post infection from irradiated female mice reconstituted with Fbxo7 +/+ (10 mice) or Fbxo7 tm1a/tm1a bone marrow (9 mice). (H-L) plasma clinical chemistry parameters at day 6 post infection from male mice (5 mice/genotype) for plasma levels of amylase, lipase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and ferritin (P values calculated from unpaired two-tailed students t tests with Welch’s correction). For panels C, D and G-L symbols represent individual mice with the line at the mean and error bars representing the standard error of the mean (D and G) or standard deviation (C, H-L). For panel F symbols represent the mean with error bars representing the standard error of the mean.

    Article Snippet: Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples).

    Techniques: Infection, Mouse Assay, Irradiation, AST Assay, Two Tailed Test, Standard Deviation

    Fbxo7 has a T cell intrinsic role in controlling T cell number in the periphery but not phenotype. (A) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. (B) T cell percentage in the spleen and (C) splenic T cell effector percentage. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction. All data representative of three independent experiments (spleen 16–20 week old female mice, thymus 12–14 week old male mice), each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.

    Journal: PLoS ONE

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    doi: 10.1371/journal.pone.0212481

    Figure Lengend Snippet: Fbxo7 has a T cell intrinsic role in controlling T cell number in the periphery but not phenotype. (A) T cell developmental stages in the thymus, DN = CD4/CD8 double negative, DP = CD4/CD8 double positive, CD4 SP = CD4 single positive and CD8 SP = CD8 single positive. (B) T cell percentage in the spleen and (C) splenic T cell effector percentage. P values from an unpaired two-tailed students t test with Holm-Sidak multiple testing correction. All data representative of three independent experiments (spleen 16–20 week old female mice, thymus 12–14 week old male mice), each symbol represents an individual mouse with the line at the mean and error bars represent standard error of the mean.

    Article Snippet: Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples).

    Techniques: Two Tailed Test, Mouse Assay

    Defects in the testis and epididymis of Fbxo7 tm1a/tm1a mice. (A) Image of epididymal spermatozoa smear stained with hemacolor from 12-14-week-old mice. (B) H E stained section of epididymis from 20-week-old mice. (C) testis weight from 18-19-week-old mice. (D) semithin toloudine blue stained section image of testis from 8-week-old mice. Images are representative of at least two mice per genotype, P value from an unpaired two-tailed students t test with Welch’s correction with each symbol representing a testis, line is at the mean and error bars represent standard deviation.

    Journal: PLoS ONE

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    doi: 10.1371/journal.pone.0212481

    Figure Lengend Snippet: Defects in the testis and epididymis of Fbxo7 tm1a/tm1a mice. (A) Image of epididymal spermatozoa smear stained with hemacolor from 12-14-week-old mice. (B) H E stained section of epididymis from 20-week-old mice. (C) testis weight from 18-19-week-old mice. (D) semithin toloudine blue stained section image of testis from 8-week-old mice. Images are representative of at least two mice per genotype, P value from an unpaired two-tailed students t test with Welch’s correction with each symbol representing a testis, line is at the mean and error bars represent standard deviation.

    Article Snippet: Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples).

    Techniques: Mouse Assay, Staining, Two Tailed Test, Standard Deviation

    Anaemia in Fbxo7 targeted mice is regenerative with a shorter erythrocyte half-life that is haematopoietic intrinsic. (A) plasma bilirubin and (B) plasma iron from 15-17-week-old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. (C) In vivo half-life of Fbxo7 +/+ and Fbxo7 tm1a/tm1a erythrocytes, the P value indicated is for each time point. D-H) characterisation of erythrocyte indices from Fbxo7 +/+ and Fbxo7 tm1a/tm1a bone marrow chimeras 10 weeks after reconstitution (female recipient mice). All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation, except for (C) where n = 6 for Fbxo7 +/+ and Fbxo7 tm1a/tm1a with mean ± standard error of the mean. P values from an unpaired two-tailed students t test with Welch’s correction (A, B, D-H) or C two-way repeated measures ANOVA with Sidak post-hoc test for individual time points.

    Journal: PLoS ONE

    Article Title: FBXO7 sensitivity of phenotypic traits elucidated by a hypomorphic allele

    doi: 10.1371/journal.pone.0212481

    Figure Lengend Snippet: Anaemia in Fbxo7 targeted mice is regenerative with a shorter erythrocyte half-life that is haematopoietic intrinsic. (A) plasma bilirubin and (B) plasma iron from 15-17-week-old Fbxo7 +/+ and Fbxo7 tm1a/tm1a female mice. (C) In vivo half-life of Fbxo7 +/+ and Fbxo7 tm1a/tm1a erythrocytes, the P value indicated is for each time point. D-H) characterisation of erythrocyte indices from Fbxo7 +/+ and Fbxo7 tm1a/tm1a bone marrow chimeras 10 weeks after reconstitution (female recipient mice). All data representative of three independent experiments, each symbol represents an individual mouse with the line at the mean and error bars represent standard deviation, except for (C) where n = 6 for Fbxo7 +/+ and Fbxo7 tm1a/tm1a with mean ± standard error of the mean. P values from an unpaired two-tailed students t test with Welch’s correction (A, B, D-H) or C two-way repeated measures ANOVA with Sidak post-hoc test for individual time points.

    Article Snippet: Fbxo7 gene expression was assessed using FAM-conjugated TaqMan assays (Mm00462692_m1 for exons 3–4 spanning the inserted cassette for the tm1a allele or the downstream exon 5–6 assay Mm01240794_m1 for tm1b samples).

    Techniques: Mouse Assay, In Vivo, Standard Deviation, Two Tailed Test