gene exp cygb hs00370478 m1  (Thermo Fisher)


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    Thermo Fisher gene exp cygb hs00370478 m1
    Quantitative real-time RT-PCR analysis of <t>HO-1</t> (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * P
    Gene Exp Cygb Hs00370478 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp cygb hs00370478 m1/product/Thermo Fisher
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gene exp cygb hs00370478 m1 - by Bioz Stars, 2020-08
    87/100 stars

    Images

    1) Product Images from "Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells"

    Article Title: Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells

    Journal: Journal of Biomedicine and Biotechnology

    doi: 10.1155/2010/395785

    Quantitative real-time RT-PCR analysis of HO-1 (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * P
    Figure Legend Snippet: Quantitative real-time RT-PCR analysis of HO-1 (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * P

    Techniques Used: Quantitative RT-PCR

    2) Product Images from "Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues"

    Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

    Journal: Cancer Cell International

    doi: 10.1186/1475-2867-10-33

    Expression of Ngb and Cygb in human astrocytomas . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. Sections of representative examples of Grades I-IV astrocytoma are shown.
    Figure Legend Snippet: Expression of Ngb and Cygb in human astrocytomas . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. Sections of representative examples of Grades I-IV astrocytoma are shown.

    Techniques Used: Expressing, Staining

    Cygb protein expression in human GBM cells . Cygb expression was assessed by Western blot analyses after exposure to hypoxia (0.6% O 2 ) for 0, 6, 24 and 48 h (n = 4). The integrated intensities of Cygb and α- tubulin (control) bands were determined and expressed in arbitrary units (AU), and representative blots are shown. (A) M006x: (B) M006xLo; (C) M010B; (D) M059J; (E) U87T. * p
    Figure Legend Snippet: Cygb protein expression in human GBM cells . Cygb expression was assessed by Western blot analyses after exposure to hypoxia (0.6% O 2 ) for 0, 6, 24 and 48 h (n = 4). The integrated intensities of Cygb and α- tubulin (control) bands were determined and expressed in arbitrary units (AU), and representative blots are shown. (A) M006x: (B) M006xLo; (C) M010B; (D) M059J; (E) U87T. * p

    Techniques Used: Expressing, Western Blot

    Expression of Ngb, Cygb and CA IX in human normal tissues . Tissue microarrays containing cores obtained from various human normal tissues were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) stomach (fundus); (B) small bowel; (C) gallbladder.
    Figure Legend Snippet: Expression of Ngb, Cygb and CA IX in human normal tissues . Tissue microarrays containing cores obtained from various human normal tissues were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) stomach (fundus); (B) small bowel; (C) gallbladder.

    Techniques Used: Expressing, Staining

    Expression of Ngb, Cygb and CA IX in human tumors . Tissue microarrays containing cores obtained from various human tumors were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) ovarian carcinoma; (B) hepatocellular carcinoma; (C) breast infiltrating duct carcinoma.
    Figure Legend Snippet: Expression of Ngb, Cygb and CA IX in human tumors . Tissue microarrays containing cores obtained from various human tumors were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) ovarian carcinoma; (B) hepatocellular carcinoma; (C) breast infiltrating duct carcinoma.

    Techniques Used: Expressing, Staining

    Expression of Cygb and Ngb in human primary brain tumors . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) oligodendroglioma; (B) ependymoblastoma; (C) ganglioglioma.
    Figure Legend Snippet: Expression of Cygb and Ngb in human primary brain tumors . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) oligodendroglioma; (B) ependymoblastoma; (C) ganglioglioma.

    Techniques Used: Expressing, Staining

    Cygb mRNA expression in human GBM cells . Cygb mRNA expression was assessed by qRT-PCR after exposure to hypoxia (0.6% O 2 ) for 0, 6, 24 and 48 h. Data were expressed as fold increase relative to aerobic control (n = 4). (A) M006x: (B) M006xLo; (C) M010B; (D) M059J; (E) U87T. * p
    Figure Legend Snippet: Cygb mRNA expression in human GBM cells . Cygb mRNA expression was assessed by qRT-PCR after exposure to hypoxia (0.6% O 2 ) for 0, 6, 24 and 48 h. Data were expressed as fold increase relative to aerobic control (n = 4). (A) M006x: (B) M006xLo; (C) M010B; (D) M059J; (E) U87T. * p

    Techniques Used: Expressing, Quantitative RT-PCR

    Related Articles

    Polymerase Chain Reaction:

    Article Title: A Topical Zinc Ionophore Blocks Tumorigenic Progression in UV-exposed SKH-1 High Risk Mouse Skin
    Article Snippet: .. Each PCR reaction contained 3.75 µL of cDNA, 12.5 µL TaqMan Universal PCR Master Mix (Roche Molecular Systems), 1.25 µL of gene specific primer (Applied Biosystems, Branchburg, NJ): human HSPA6 (Hs00275682_s1), HSPA1A (Hs00359163_s1), HMOX1 (Hs00157965_m1), DDIT3 (Hs00358796_g1), MT2A (Hs02379661_g1), GADD45A (Hs00169255_m1), and ACTB (Hs99999903_m1). .. Gene-specific product was normalized to ACTB and quantified using the comparative (ΔΔCt ) Ct method following the ABI Prism 7000 sequence detection system user manual as described before ( , , ).

    Expressing:

    Article Title: Levels of circulating myeloid subpopulations and of heme oxygenase-1 do not predict CD4+ T cell recovery after the initiation of antiretroviral therapy for HIV disease
    Article Snippet: .. Relative expression levels of HMOX1 mRNA were measured by quantitative RT-PCR using validated Taqman® Gene Expression assay mixes for human HMOX1 (Hs00157965_m1) and human HPRT (Hs99999909_m1) according to the manufacturer’s protocol (Applied Biosystems). .. An AB Step One Plus instrument was used for amplification and detection, and the 2-ΔΔCT calculation was used to measure HMOX1 gene expression relative to HPRT [ ].

    Quantitative RT-PCR:

    Article Title: Levels of circulating myeloid subpopulations and of heme oxygenase-1 do not predict CD4+ T cell recovery after the initiation of antiretroviral therapy for HIV disease
    Article Snippet: .. Relative expression levels of HMOX1 mRNA were measured by quantitative RT-PCR using validated Taqman® Gene Expression assay mixes for human HMOX1 (Hs00157965_m1) and human HPRT (Hs99999909_m1) according to the manufacturer’s protocol (Applied Biosystems). .. An AB Step One Plus instrument was used for amplification and detection, and the 2-ΔΔCT calculation was used to measure HMOX1 gene expression relative to HPRT [ ].

    Article Title: ACTIVATION OF DIOXIN RESPONSE ELEMENT (DRE)-ASSOCIATED GENES BY BENZO(A)PYRENE 3,6-QUINONE AND BENZO(A)PYRENE 1,6-QUINONE IN MCF-10A HUMAN MAMMARY EPITHELIAL CELLS
    Article Snippet: .. Gene induction of seven genes, heme oxygenase 1 (HMOX-1; Assay ID HS00157965_m1), NAD(P)H dehydrogenase, quinone 1 (NQO1; Assay ID HS00168547_m1), epoxide hydrolase 1 (EPHX1; Assay ID HS00164458_m1), cytochrome P450, subfamily 1A1 (CYP1A1, Assay ID HS00153120_m1), cytochrome P450, subfamily 1B1 (CYP1B1, Assay ID HS00164383_m1), aldehyde dehydrogenase 3 family member A1 (ALDH3A1, Assay ID HS00167469_m1) and aldo-ketoreductase family 1 member C1 (AKR1C1, Assay ID HS00413886_m1) were measured by qRT-PCR. .. TaqMan® Gene Expression Assays for each of the above genes were purchased from Applied Biosystems (Foster City, CA).

    Article Title: The redox antimalarial dihydroartemisinin targets human metastatic melanoma cells but not primary melanocytes with induction of NOXA-dependent apoptosis
    Article Snippet: .. After reverse transcription, real time RT-PCR was performed using an Applied Biosystems 7000 SDS and Applied Biosystems’ Assays On Demand primers specific to CDKN1A (assay ID Hs00355782_m1), DDIT3 (assay ID Hs00358796_g1), GADD45A (assay ID Hs00169255_m1), HMOX1 (assay ID Hs00157965_m1), PMAIP1 (assay ID Hs00560402_m1), and GAPDH (assay ID Hs99999905_m1). .. Gene-specific product was normalized to GAPDH and quantified using the comparative (ΔΔCt ) Ct method as described in the ABI Prism 7000 sequence detection system user guide [ ].

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  • 87
    Thermo Fisher gene exp cygb hs00370478 m1
    Quantitative real-time RT-PCR analysis of <t>HO-1</t> (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * P
    Gene Exp Cygb Hs00370478 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp cygb hs00370478 m1/product/Thermo Fisher
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gene exp cygb hs00370478 m1 - by Bioz Stars, 2020-08
    87/100 stars
      Buy from Supplier

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    Quantitative real-time RT-PCR analysis of HO-1 (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * P

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells

    doi: 10.1155/2010/395785

    Figure Lengend Snippet: Quantitative real-time RT-PCR analysis of HO-1 (a), AQP-1 (b), and Bcl-2 (c) expressions in mixed hTECs under basal conditions and after GO-induced oxidative stress (1 IU/mL) either or not in the presence of epoetin delta (100 IU/mL). GAPDH was used as endogenous control housekeeping gene. Preconditioning the cells with EPO resulted in a significant upregulation of HO-1 and AQP-1 mRNA. GO-induced oxidative stress further increased HO-1, AQP-1, and Bcl-2 mRNA expressions with maximum levels 60 minutes after induction of oxidative stress. Data are presented as the mean ± SD of triplicate determinations of 2 runs (i.e., 6 values each). * P

    Article Snippet: Ready to use, predesigned, primer and probe sets (Applied Biosystems) for human genes of interest (Hs00157965_m1 for HO-1, Hs00166067_m1 for AQP-1, Hs00153350_m1 for Bcl-2, Hs00266395_m1 for CPM, Hs00175210_m1 for DPPIV, Hs00370478_m1 for Cygb) and the housekeeping gene GAPDH (Hs99999905_m1) were used according to the manufacturer's guidelines.

    Techniques: Quantitative RT-PCR