gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    Gene expression primer and probe sets used for RT-qPCR.
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Different effects of valproic acid on photoreceptor loss in Rd1 and Rd10 retinal degeneration mice"

    Article Title: Different effects of valproic acid on photoreceptor loss in Rd1 and Rd10 retinal degeneration mice

    Journal: Molecular Vision

    doi:

    Gene expression primer and probe sets used for RT-qPCR.
    Figure Legend Snippet: Gene expression primer and probe sets used for RT-qPCR.

    Techniques Used: Expressing

    Effect of a single systemic dose of valproic acid (VPA) on neurotrophic factor gene expression and rod-specific gene expression in the immature retina of wild-type mice. A : Expression of the Gdnf , Bdnf, and Cntf genes in the neural retinas of wild-type mice (C57BL/6) was examined at two postnatal ages, P12 (n = 2/group) and P15 (n = 3/group), 18 h after one systemic VPA dose. Littermates at each age received a high dose (415 mg/kg), low dose (250 mg/kg), or PBS. The graph shows the average of these experiments (mean±standard deviation [SD]). Gene expression was normalized to endogenous Actb expression and is plotted relative to control PBS-injected littermates ( t test, * p<0.01, ** p<0.001, relative to PBS). B : Retinal Nrl gene expression at two postnatal ages, P12 (n = 2/group) and P15 (n = 3/group), 18 h after a single systemic injection (IP) of VPA. Littermates within each age received a high dose (VPA 415 mg/kg), low dose (VPA 250 mg/kg), or PBS only (control). The graph shows the average of the two experiments (mean±SD; t test, *p<0.05 relative to PBS). C : Rod-specific Mef2c gene expression at age P15, 18 h after treatment with a high dose (VPA 415 mg/kg), low dose (VPA 250 mg/kg), or control (PBS) ( t test, *p<0.05 relative to PBS, n = 3/group). D : Rhodopsin gene ( Rho ) expression in the P12 neural retina, 18 h after injection (IP) with a high or low dose of VPA compared to PBS only (mean±SD; n = 2/group).
    Figure Legend Snippet: Effect of a single systemic dose of valproic acid (VPA) on neurotrophic factor gene expression and rod-specific gene expression in the immature retina of wild-type mice. A : Expression of the Gdnf , Bdnf, and Cntf genes in the neural retinas of wild-type mice (C57BL/6) was examined at two postnatal ages, P12 (n = 2/group) and P15 (n = 3/group), 18 h after one systemic VPA dose. Littermates at each age received a high dose (415 mg/kg), low dose (250 mg/kg), or PBS. The graph shows the average of these experiments (mean±standard deviation [SD]). Gene expression was normalized to endogenous Actb expression and is plotted relative to control PBS-injected littermates ( t test, * p<0.01, ** p<0.001, relative to PBS). B : Retinal Nrl gene expression at two postnatal ages, P12 (n = 2/group) and P15 (n = 3/group), 18 h after a single systemic injection (IP) of VPA. Littermates within each age received a high dose (VPA 415 mg/kg), low dose (VPA 250 mg/kg), or PBS only (control). The graph shows the average of the two experiments (mean±SD; t test, *p<0.05 relative to PBS). C : Rod-specific Mef2c gene expression at age P15, 18 h after treatment with a high dose (VPA 415 mg/kg), low dose (VPA 250 mg/kg), or control (PBS) ( t test, *p<0.05 relative to PBS, n = 3/group). D : Rhodopsin gene ( Rho ) expression in the P12 neural retina, 18 h after injection (IP) with a high or low dose of VPA compared to PBS only (mean±SD; n = 2/group).

    Techniques Used: Expressing, Standard Deviation, Injection

    Effect of a single systemic dose of valproic acid (VPA) on retinal neurotrophic factor gene expression in the Pde6b rd1/rd1 mouse strain. A single dose of VPA (350 mg/kg) was administered by intraperitoneal injection, and neural retinas were collected 18 h after the injection at age P15. Gene expression relative to PBS-injected littermates was measured using real-time PCR for the gene of interest ( Gdnf, Bdnf, Cntf, Fgf2 ) and was normalized to Actb expression (mean±standard deviation; n = 3/group, t test, *p<0.08, **p<0.005, ***p<0.001, relative to PBS).
    Figure Legend Snippet: Effect of a single systemic dose of valproic acid (VPA) on retinal neurotrophic factor gene expression in the Pde6b rd1/rd1 mouse strain. A single dose of VPA (350 mg/kg) was administered by intraperitoneal injection, and neural retinas were collected 18 h after the injection at age P15. Gene expression relative to PBS-injected littermates was measured using real-time PCR for the gene of interest ( Gdnf, Bdnf, Cntf, Fgf2 ) and was normalized to Actb expression (mean±standard deviation; n = 3/group, t test, *p<0.08, **p<0.005, ***p<0.001, relative to PBS).

    Techniques Used: Expressing, Injection, Real-time Polymerase Chain Reaction, Standard Deviation

    Effect of daily systemic valproic acid (VPA; P17–P28, late treatment) on neurotrophic factor and rod-specific gene expression in the wild-type mouse retina. A daily dose of VPA (350 mg/kg) was administered by intraperitoneal injection starting at age P17. Littermates received injections of PBS only. Gene expression, relative to PBS-injected littermates at age P28 was measured using real-time PCR for the gene of interest ( Gdnf, Bdnf, Cntf, Fgf2, Mef2c, Nrl, Crx, Nr2E3, Rho, Pde6b ) and was normalized to Actb expression (mean±standard deviation; n = 3/group, t test, *p<0.05, **p<0.01, relative to PBS).
    Figure Legend Snippet: Effect of daily systemic valproic acid (VPA; P17–P28, late treatment) on neurotrophic factor and rod-specific gene expression in the wild-type mouse retina. A daily dose of VPA (350 mg/kg) was administered by intraperitoneal injection starting at age P17. Littermates received injections of PBS only. Gene expression, relative to PBS-injected littermates at age P28 was measured using real-time PCR for the gene of interest ( Gdnf, Bdnf, Cntf, Fgf2, Mef2c, Nrl, Crx, Nr2E3, Rho, Pde6b ) and was normalized to Actb expression (mean±standard deviation; n = 3/group, t test, *p<0.05, **p<0.01, relative to PBS).

    Techniques Used: Expressing, Injection, Real-time Polymerase Chain Reaction, Standard Deviation

    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    List of genes selected for validation by qPCR analysis.
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Analysis of the Transcriptome in Hyperoxic Lung Injury and Sex-Specific Alterations in Gene Expression"

    Article Title: Analysis of the Transcriptome in Hyperoxic Lung Injury and Sex-Specific Alterations in Gene Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0101581

    List of genes selected for validation by qPCR analysis.
    Figure Legend Snippet: List of genes selected for validation by qPCR analysis.

    Techniques Used:

    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    Oligonucleotide primers used for RT-qPCR based on TaqMan®
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The neuropeptide PACAP alleviates T. gondii infection-induced neuroinflammation and neuronal impairment"

    Article Title: The neuropeptide PACAP alleviates T. gondii infection-induced neuroinflammation and neuronal impairment

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-022-02639-z

    Oligonucleotide primers used for RT-qPCR based on TaqMan®
    Figure Legend Snippet: Oligonucleotide primers used for RT-qPCR based on TaqMan®

    Techniques Used: Expressing

    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    A , B RNA-sequence analysis of the prefrontal cortex (PFC) of CRS-exposed mice treated with ( R )-ketamine (10 mg/kg) ( n = 5) or saline ( n = 5). The differentially expressed miRNAs were shown in the ( A : fold of the change) and ( B : P value). C Bioinformatics prediction by TargetScanMouse showed that miR-132-5p can regulate <t>BDNF</t> and TGF-β1. D Expression of miR-132-5p in the PFC (one way ANOVA: F 2,28 = 19.27, P < 0.0001). E Bdnf mRNA in the PFC (one way ANOVA: F 2,28 = 13.34, P < 0.0001). F Mecp2 mRNA in the PFC (one way ANOVA: F 2,28 = 14.47, P < 0.0001). G Tgfb1 mRNA in the PFC (one way ANOVA: F 2,28 = 13.49, P < 0.0001). H Tgfbr2 mRNA in the PFC (one way ANOVA: F 2,28 = 12.14, P = 0.0002). The data represent mean ± SEM ( n = 10 or 11). * P < 0.05, ** P < 0.01, *** P < 0.001.
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A key role of miR-132-5p in the prefrontal cortex for persistent prophylactic actions of ( R )-ketamine in mice"

    Article Title: A key role of miR-132-5p in the prefrontal cortex for persistent prophylactic actions of ( R )-ketamine in mice

    Journal: Translational Psychiatry

    doi: 10.1038/s41398-022-02192-6

    A , B RNA-sequence analysis of the prefrontal cortex (PFC) of CRS-exposed mice treated with ( R )-ketamine (10 mg/kg) ( n = 5) or saline ( n = 5). The differentially expressed miRNAs were shown in the ( A : fold of the change) and ( B : P value). C Bioinformatics prediction by TargetScanMouse showed that miR-132-5p can regulate BDNF and TGF-β1. D Expression of miR-132-5p in the PFC (one way ANOVA: F 2,28 = 19.27, P < 0.0001). E Bdnf mRNA in the PFC (one way ANOVA: F 2,28 = 13.34, P < 0.0001). F Mecp2 mRNA in the PFC (one way ANOVA: F 2,28 = 14.47, P < 0.0001). G Tgfb1 mRNA in the PFC (one way ANOVA: F 2,28 = 13.49, P < 0.0001). H Tgfbr2 mRNA in the PFC (one way ANOVA: F 2,28 = 12.14, P = 0.0002). The data represent mean ± SEM ( n = 10 or 11). * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A , B RNA-sequence analysis of the prefrontal cortex (PFC) of CRS-exposed mice treated with ( R )-ketamine (10 mg/kg) ( n = 5) or saline ( n = 5). The differentially expressed miRNAs were shown in the ( A : fold of the change) and ( B : P value). C Bioinformatics prediction by TargetScanMouse showed that miR-132-5p can regulate BDNF and TGF-β1. D Expression of miR-132-5p in the PFC (one way ANOVA: F 2,28 = 19.27, P < 0.0001). E Bdnf mRNA in the PFC (one way ANOVA: F 2,28 = 13.34, P < 0.0001). F Mecp2 mRNA in the PFC (one way ANOVA: F 2,28 = 14.47, P < 0.0001). G Tgfb1 mRNA in the PFC (one way ANOVA: F 2,28 = 13.49, P < 0.0001). H Tgfbr2 mRNA in the PFC (one way ANOVA: F 2,28 = 12.14, P = 0.0002). The data represent mean ± SEM ( n = 10 or 11). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Sequencing, Expressing

    A The protein expression of brain-derived neurotrophic factor (BDNF) (one way ANOVA: F 2,27 = 6.985, P = 0.004). B Methyl-CpG-binding protein 2 (MeCP2) (one way ANOVA: F 2,27 = 5.530, P = 0.010). C Transforming growth factor (TGF)-β1 (one way ANOVA: F 2,27 = 7.523, P = 0.003). D Postsynaptic protein-95 (PSD-95) (one way ANOVA: F 2,27 = 4.888, P = 0.015). E AMPA Receptor 1 (GluA1) (one way ANOVA: F 2,27 = 4.825, P = 0.016) in the prefrontal cortex (PFC). The data represent mean ± SEM ( n = 9–11). * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A The protein expression of brain-derived neurotrophic factor (BDNF) (one way ANOVA: F 2,27 = 6.985, P = 0.004). B Methyl-CpG-binding protein 2 (MeCP2) (one way ANOVA: F 2,27 = 5.530, P = 0.010). C Transforming growth factor (TGF)-β1 (one way ANOVA: F 2,27 = 7.523, P = 0.003). D Postsynaptic protein-95 (PSD-95) (one way ANOVA: F 2,27 = 4.888, P = 0.015). E AMPA Receptor 1 (GluA1) (one way ANOVA: F 2,27 = 4.825, P = 0.016) in the prefrontal cortex (PFC). The data represent mean ± SEM ( n = 9–11). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Expressing, Derivative Assay, Binding Assay

    A Treatment schedule. AgomiR-132-5p (200 nmol, 2 μl/day) or agomiR-NC (control: 2 μl/day) was administered i.c.v. to mice for 3 days (day 1–day 3). Locomotion test (LMT), forced swimming test (FST) and sucrose preference test (SPT) were performed after the final injection of agomiR-NC or agomiR-132-5p (day 4 and day 5). The prefrontal cortex (PFC) was collected after SPT. B Body weight change (two-way repeated measures ANOVA: F 2,36 = 3.765, P = 0.033). C Locomotion test (unpaired Student t-test: P = 0.129). D FST (unpaired Student t-test: P = 0.034). E SPT (unpaired Student t-test: P = 0.012). F Expression of miR-132-5p in the PFC (unpaired Student t-test: P = 0.033). G Bdnf mRNA in the PFC (unpaired Student t-test: P = 0.0005). ( H ): Tgfb1 mRNA in the PFC (unpaired Student t-test: P = 0.018). The data represent mean ± SEM ( n = 10). * P < 0.05, ** P < 0.01, *** P < 0.001. N.S., not significant.
    Figure Legend Snippet: A Treatment schedule. AgomiR-132-5p (200 nmol, 2 μl/day) or agomiR-NC (control: 2 μl/day) was administered i.c.v. to mice for 3 days (day 1–day 3). Locomotion test (LMT), forced swimming test (FST) and sucrose preference test (SPT) were performed after the final injection of agomiR-NC or agomiR-132-5p (day 4 and day 5). The prefrontal cortex (PFC) was collected after SPT. B Body weight change (two-way repeated measures ANOVA: F 2,36 = 3.765, P = 0.033). C Locomotion test (unpaired Student t-test: P = 0.129). D FST (unpaired Student t-test: P = 0.034). E SPT (unpaired Student t-test: P = 0.012). F Expression of miR-132-5p in the PFC (unpaired Student t-test: P = 0.033). G Bdnf mRNA in the PFC (unpaired Student t-test: P = 0.0005). ( H ): Tgfb1 mRNA in the PFC (unpaired Student t-test: P = 0.018). The data represent mean ± SEM ( n = 10). * P < 0.05, ** P < 0.01, *** P < 0.001. N.S., not significant.

    Techniques Used: Injection, Expressing

    A Treatment schedule. Chronic restraint stress (CRS) was performed 7 days (day 1–day 7). Subsequently, antagomiR-132-5p (200 nmol, 2 μl/day) or antagomiR-NC (control: 2 μl/day) was administered i.c.v. to mice 3 days after CRS (day 8–day 10). Locomotion test, forced swimming test (FST) and sucrose preference test (SPT) were performed after the injection of antagomiR-NC or antagomiR-132-5p (day 11 and day 12). The prefrontal cortex (PFC) was collected after SPT. B Body weight change (two-way repeated measures ANOVA: F 4,52 = 1,125, P = 0.300). C Locomotion test (one-way ANOVA: F 2,26 = 0.587, P = 0.563). D FST (one-way ANOVA: F 2,26 = 6.709, P = 0.005). E SPT (one-way ANOVA: F 2,26 = 5.581, P = 0.010). F Expression of miR-132-5p in the PFC (one-way ANOVA: F 2,26 = 10.470, P = 0.0005). G Bdnf mRNA in the PFC (one-way ANOVA: F 2,26 = 15.740, P < 0.0001). The data represent mean ± SEM ( n = 9 or 10). * P < 0.05, ** P < 0.01, *** P < 0.001. N.S., not significant.
    Figure Legend Snippet: A Treatment schedule. Chronic restraint stress (CRS) was performed 7 days (day 1–day 7). Subsequently, antagomiR-132-5p (200 nmol, 2 μl/day) or antagomiR-NC (control: 2 μl/day) was administered i.c.v. to mice 3 days after CRS (day 8–day 10). Locomotion test, forced swimming test (FST) and sucrose preference test (SPT) were performed after the injection of antagomiR-NC or antagomiR-132-5p (day 11 and day 12). The prefrontal cortex (PFC) was collected after SPT. B Body weight change (two-way repeated measures ANOVA: F 4,52 = 1,125, P = 0.300). C Locomotion test (one-way ANOVA: F 2,26 = 0.587, P = 0.563). D FST (one-way ANOVA: F 2,26 = 6.709, P = 0.005). E SPT (one-way ANOVA: F 2,26 = 5.581, P = 0.010). F Expression of miR-132-5p in the PFC (one-way ANOVA: F 2,26 = 10.470, P = 0.0005). G Bdnf mRNA in the PFC (one-way ANOVA: F 2,26 = 15.740, P < 0.0001). The data represent mean ± SEM ( n = 9 or 10). * P < 0.05, ** P < 0.01, *** P < 0.001. N.S., not significant.

    Techniques Used: Injection, Expressing

    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    qRT–PCR results showing the effect of EGCG and Anafranil on the hippocampal <t>BDNF</t> level in CUMS-exposed mice. The bars represent the means ± SEs ( n = 3). * p < 0.05 compared with control; # p < 0.05 compared with CUMS
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Effect of Epigallocatechin-3-gallate on Stress-Induced Depression in a Mouse Model: Role of Interleukin-1β and Brain-Derived Neurotrophic Factor"

    Article Title: Effect of Epigallocatechin-3-gallate on Stress-Induced Depression in a Mouse Model: Role of Interleukin-1β and Brain-Derived Neurotrophic Factor

    Journal: Neurochemical Research

    doi: 10.1007/s11064-022-03707-9

    qRT–PCR results showing the effect of EGCG and Anafranil on the hippocampal BDNF level in CUMS-exposed mice. The bars represent the means ± SEs ( n = 3). * p < 0.05 compared with control; # p < 0.05 compared with CUMS
    Figure Legend Snippet: qRT–PCR results showing the effect of EGCG and Anafranil on the hippocampal BDNF level in CUMS-exposed mice. The bars represent the means ± SEs ( n = 3). * p < 0.05 compared with control; # p < 0.05 compared with CUMS

    Techniques Used: Quantitative RT-PCR

    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp bdnf mm04230607 s1
    ( A ) Schematic mechanism of action of afatinib, UNC2025, and pemigatinib. ( B ) Ccl2 , Ccl5 , Tnf , Nos2 , Lif , and Ngf expression, quantified by reverse transcriptase qPCR (RT-qPCR) in primary mouse astrocytes after stimulation (stim) with TNF-α and IL-1β, IL-6, IFN-γ, or GM-CSF with or without afatinib, UNC2025, and pemigatinib; n = 4 per group. Two-way ANOVA with Dunnett’s multiple comparisons test. ( C ) iNOS, TNF-α, GM-CSF, and Ki-67 were quantified by intracellular flow cytometry of primary mouse astrocytes after stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib. Data are representative for n = 2 independent experiments with n = 4 per group. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( D ) ELISA measurement of NGF and <t>BDNF</t> produced by primary mouse astrocytes after stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib; n = 2. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( E ) CCL2 , NOS2 , and LIF expression quantified by RT-qPCR in human astrocytes with or without stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib; n = 4. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( F ) Relative expression of Il6 , and Ccl2 in primary mouse microglia quantified by RT-qPCR after stim with ACM derived from unstimulated and stim (TNF-α and IL-1β) primary mouse astrocytes with or without afatinib, UNC2025, and pemigatinib; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( G ) Annexin V propidium iodide apoptosis assay of neuronal N2A cells stimulated with ACM; n = 3. Cells were categorized as in early or late apoptosis. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( H ) Migration assay of CD11b + myeloid cells stimulated with ACM; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Intranasal delivery of a small-molecule ErbB inhibitor promotes recovery from acute and late-stage CNS inflammation"

    Article Title: Intranasal delivery of a small-molecule ErbB inhibitor promotes recovery from acute and late-stage CNS inflammation

    Journal: JCI Insight

    doi: 10.1172/jci.insight.154824

    ( A ) Schematic mechanism of action of afatinib, UNC2025, and pemigatinib. ( B ) Ccl2 , Ccl5 , Tnf , Nos2 , Lif , and Ngf expression, quantified by reverse transcriptase qPCR (RT-qPCR) in primary mouse astrocytes after stimulation (stim) with TNF-α and IL-1β, IL-6, IFN-γ, or GM-CSF with or without afatinib, UNC2025, and pemigatinib; n = 4 per group. Two-way ANOVA with Dunnett’s multiple comparisons test. ( C ) iNOS, TNF-α, GM-CSF, and Ki-67 were quantified by intracellular flow cytometry of primary mouse astrocytes after stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib. Data are representative for n = 2 independent experiments with n = 4 per group. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( D ) ELISA measurement of NGF and BDNF produced by primary mouse astrocytes after stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib; n = 2. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( E ) CCL2 , NOS2 , and LIF expression quantified by RT-qPCR in human astrocytes with or without stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib; n = 4. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( F ) Relative expression of Il6 , and Ccl2 in primary mouse microglia quantified by RT-qPCR after stim with ACM derived from unstimulated and stim (TNF-α and IL-1β) primary mouse astrocytes with or without afatinib, UNC2025, and pemigatinib; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( G ) Annexin V propidium iodide apoptosis assay of neuronal N2A cells stimulated with ACM; n = 3. Cells were categorized as in early or late apoptosis. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( H ) Migration assay of CD11b + myeloid cells stimulated with ACM; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
    Figure Legend Snippet: ( A ) Schematic mechanism of action of afatinib, UNC2025, and pemigatinib. ( B ) Ccl2 , Ccl5 , Tnf , Nos2 , Lif , and Ngf expression, quantified by reverse transcriptase qPCR (RT-qPCR) in primary mouse astrocytes after stimulation (stim) with TNF-α and IL-1β, IL-6, IFN-γ, or GM-CSF with or without afatinib, UNC2025, and pemigatinib; n = 4 per group. Two-way ANOVA with Dunnett’s multiple comparisons test. ( C ) iNOS, TNF-α, GM-CSF, and Ki-67 were quantified by intracellular flow cytometry of primary mouse astrocytes after stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib. Data are representative for n = 2 independent experiments with n = 4 per group. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( D ) ELISA measurement of NGF and BDNF produced by primary mouse astrocytes after stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib; n = 2. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( E ) CCL2 , NOS2 , and LIF expression quantified by RT-qPCR in human astrocytes with or without stim with TNF-α and IL-1β with or without afatinib, UNC2025, and pemigatinib; n = 4. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( F ) Relative expression of Il6 , and Ccl2 in primary mouse microglia quantified by RT-qPCR after stim with ACM derived from unstimulated and stim (TNF-α and IL-1β) primary mouse astrocytes with or without afatinib, UNC2025, and pemigatinib; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( G ) Annexin V propidium iodide apoptosis assay of neuronal N2A cells stimulated with ACM; n = 3. Cells were categorized as in early or late apoptosis. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. ( H ) Migration assay of CD11b + myeloid cells stimulated with ACM; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test; data are reported as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Produced, Derivative Assay, Modified Annexin V/Propidium Iodide Apoptosis Assay, Migration

    ( A ) Clinical course (left) and linear regression analysis (right) of EAE in mice after daily i.n. treatment with vehicle or afatinib starting from peak of disease. The experiment was repeated twice. Vehicle (PBS), n = 7; afatinib, n = 12. Data are reported as mean ± SEM. ( B and C ) Abundance (percentage of total cell counts) of CNS cell populations during late-stage CNS inflammation in mice treated with vehicle or afatinib from symptom onset (vehicle, n = 4 or 5; afatinib, n = 4 or 5) ( B ) or peak of disease ( C ) (vehicle, n = 7; afatinib, n = 9), quantified by high-dimensional flow cytometry. Two-way ANOVA with Šidák’s multiple comparisons test. ( D and E ) Intracellular flow cytometry quantification of iNOS, TNF-α, and GM-CSF in astrocytes and microglia during late-stage CNS inflammation in mice treated with afatinib ( n = 5) or vehicle ( n = 5) from symptom onset ( D ) or peak of disease ( E ) (vehicle, n = 7; afatinib n = 8). Two-way ANOVA with Šidák’s multiple comparison test. ( F ) Relative expression of Gfap, Ngf , and Bdnf in ACSA2 + astrocytes during late-stage CNS inflammation in mice treated with afatinib ( n = 8) or vehicle ( n = 7) from peak of disease. Unpaired t test with Welch’s correction; data are reported as mean ± SD. ( G and H ) Representative fluorescence images ( G ) and quantification ( H ) of immunohistochemically labeled Olig2 + oligodendrocytes in lumbar spinal cord of mice treated with vehicle ( n = 5) or afatinib ( n = 5). Scale bars: 15 μm. Two-way ANOVA with Dunnett’s multiple comparisons test. ( I and J ) Representative fluorescence images ( I ) and corrected total cell fluorescence (CTCF) quantification ( J ) of axonal damage (SMI32 + ) in lumbar spinal cord of mice treated with vehicle ( n = 5) or afatinib ( n = 5). Two-way ANOVA with Dunnett’s multiple comparisons test. Scale bars: 15 μm. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. p.i., postimmunization.
    Figure Legend Snippet: ( A ) Clinical course (left) and linear regression analysis (right) of EAE in mice after daily i.n. treatment with vehicle or afatinib starting from peak of disease. The experiment was repeated twice. Vehicle (PBS), n = 7; afatinib, n = 12. Data are reported as mean ± SEM. ( B and C ) Abundance (percentage of total cell counts) of CNS cell populations during late-stage CNS inflammation in mice treated with vehicle or afatinib from symptom onset (vehicle, n = 4 or 5; afatinib, n = 4 or 5) ( B ) or peak of disease ( C ) (vehicle, n = 7; afatinib, n = 9), quantified by high-dimensional flow cytometry. Two-way ANOVA with Šidák’s multiple comparisons test. ( D and E ) Intracellular flow cytometry quantification of iNOS, TNF-α, and GM-CSF in astrocytes and microglia during late-stage CNS inflammation in mice treated with afatinib ( n = 5) or vehicle ( n = 5) from symptom onset ( D ) or peak of disease ( E ) (vehicle, n = 7; afatinib n = 8). Two-way ANOVA with Šidák’s multiple comparison test. ( F ) Relative expression of Gfap, Ngf , and Bdnf in ACSA2 + astrocytes during late-stage CNS inflammation in mice treated with afatinib ( n = 8) or vehicle ( n = 7) from peak of disease. Unpaired t test with Welch’s correction; data are reported as mean ± SD. ( G and H ) Representative fluorescence images ( G ) and quantification ( H ) of immunohistochemically labeled Olig2 + oligodendrocytes in lumbar spinal cord of mice treated with vehicle ( n = 5) or afatinib ( n = 5). Scale bars: 15 μm. Two-way ANOVA with Dunnett’s multiple comparisons test. ( I and J ) Representative fluorescence images ( I ) and corrected total cell fluorescence (CTCF) quantification ( J ) of axonal damage (SMI32 + ) in lumbar spinal cord of mice treated with vehicle ( n = 5) or afatinib ( n = 5). Two-way ANOVA with Dunnett’s multiple comparisons test. Scale bars: 15 μm. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. p.i., postimmunization.

    Techniques Used: Flow Cytometry, Expressing, Fluorescence, Labeling

    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gene exp bdnf mm04230607 s1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm04230607 s1
    The mRNA expression of neurotrophic factors in different brain areas after sweetened alcohol drinking conditioning. A Number of visits in the corner. B Number of nosepokes in conditioned (CS +) side. C Number of licks on CS + side. D The ethanol dose that mice consumed during alcohol drinking conditioning was estimated as g/kg/24 h. E RT-qPCR analysis of Gdnf mRNA expression F RT-qPCR analysis of <t>Bdnf</t> mRNA expression. G RT-qPCR analysis of Manf mRNA expression. H RT-qPCR analysis of Cdnf mRNA expression
    Gene Exp Bdnf Mm04230607 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The overexpression of GDNF in nucleus accumbens suppresses alcohol-seeking behavior in group-housed C57Bl/6J female mice"

    Article Title: The overexpression of GDNF in nucleus accumbens suppresses alcohol-seeking behavior in group-housed C57Bl/6J female mice

    Journal: Journal of Biomedical Science

    doi: 10.1186/s12929-021-00782-y

    The mRNA expression of neurotrophic factors in different brain areas after sweetened alcohol drinking conditioning. A Number of visits in the corner. B Number of nosepokes in conditioned (CS +) side. C Number of licks on CS + side. D The ethanol dose that mice consumed during alcohol drinking conditioning was estimated as g/kg/24 h. E RT-qPCR analysis of Gdnf mRNA expression F RT-qPCR analysis of Bdnf mRNA expression. G RT-qPCR analysis of Manf mRNA expression. H RT-qPCR analysis of Cdnf mRNA expression
    Figure Legend Snippet: The mRNA expression of neurotrophic factors in different brain areas after sweetened alcohol drinking conditioning. A Number of visits in the corner. B Number of nosepokes in conditioned (CS +) side. C Number of licks on CS + side. D The ethanol dose that mice consumed during alcohol drinking conditioning was estimated as g/kg/24 h. E RT-qPCR analysis of Gdnf mRNA expression F RT-qPCR analysis of Bdnf mRNA expression. G RT-qPCR analysis of Manf mRNA expression. H RT-qPCR analysis of Cdnf mRNA expression

    Techniques Used: Expressing, Quantitative RT-PCR

    Behavioral activity in the automated cages during alcohol drinking conditioning and extinction tests after viral overexpression of GDNF and BDNF. A Schematic representation of the experimental timeline. B Number of visits in the corner. C Number of nosepokes in conditioned CS + side. D Number of licks in CS + side. E The ethanol dose that mice consumed during alcohol drinking conditioning was estimated as g/kg/24 h. F Spread of AAV-GFP viral vector in the nucleus accumbens is demonstrated by GFP immunohistochemistry. G Number of visits to the corner on WD1 and WD10. H Number of nosepokes in CS + and CS− sides on WD1 and WD10. I A number of licks in CS + and CS− sides on WD1 and WD10. *p < 0.05, ***p < 0.001. All means are presented with their standard errors (± SEM)
    Figure Legend Snippet: Behavioral activity in the automated cages during alcohol drinking conditioning and extinction tests after viral overexpression of GDNF and BDNF. A Schematic representation of the experimental timeline. B Number of visits in the corner. C Number of nosepokes in conditioned CS + side. D Number of licks in CS + side. E The ethanol dose that mice consumed during alcohol drinking conditioning was estimated as g/kg/24 h. F Spread of AAV-GFP viral vector in the nucleus accumbens is demonstrated by GFP immunohistochemistry. G Number of visits to the corner on WD1 and WD10. H Number of nosepokes in CS + and CS− sides on WD1 and WD10. I A number of licks in CS + and CS− sides on WD1 and WD10. *p < 0.05, ***p < 0.001. All means are presented with their standard errors (± SEM)

    Techniques Used: Activity Assay, Over Expression, Plasmid Preparation, Immunohistochemistry

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