gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    A–C Relative amounts of mRNA for NGF, <t>BDNF</t> and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Environmental Enrichment Extends Photoreceptor Survival and Visual Function in a Mouse Model of Retinitis Pigmentosa"

    Article Title: Environmental Enrichment Extends Photoreceptor Survival and Visual Function in a Mouse Model of Retinitis Pigmentosa

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050726

    A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.
    Figure Legend Snippet: A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.

    Techniques Used: Quantitative RT-PCR

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    A–C Relative amounts of mRNA for NGF, <t>BDNF</t> and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Environmental Enrichment Extends Photoreceptor Survival and Visual Function in a Mouse Model of Retinitis Pigmentosa"

    Article Title: Environmental Enrichment Extends Photoreceptor Survival and Visual Function in a Mouse Model of Retinitis Pigmentosa

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050726

    A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.
    Figure Legend Snippet: A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.

    Techniques Used: Quantitative RT-PCR

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    PCR targets
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Central administration of insulin-like growth factor-I decreases depressive-like behavior and brain cytokine expression in mice"

    Article Title: Central administration of insulin-like growth factor-I decreases depressive-like behavior and brain cytokine expression in mice

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-8-12

    PCR targets
    Figure Legend Snippet: PCR targets

    Techniques Used: Expressing

    IGF-I increases BDNF mRNA transcripts in the presence or absence of LPS . Steady-state mRNA expression for the two 3' classes of IGF-I (A, B) and two distinct BDNF transcripts (C, D) was quantified by real-time rtPCR. Expression was relative to GAPDH, which was used as a housekeeping control gene. n = 10 to 12 per treatment
    Figure Legend Snippet: IGF-I increases BDNF mRNA transcripts in the presence or absence of LPS . Steady-state mRNA expression for the two 3' classes of IGF-I (A, B) and two distinct BDNF transcripts (C, D) was quantified by real-time rtPCR. Expression was relative to GAPDH, which was used as a housekeeping control gene. n = 10 to 12 per treatment

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    Genes selected for RT-PCR.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Voluntary Wheel Running Reverses Age-Induced Changes in Hippocampal Gene Expression"

    Article Title: Voluntary Wheel Running Reverses Age-Induced Changes in Hippocampal Gene Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0022654

    Genes selected for RT-PCR.
    Figure Legend Snippet: Genes selected for RT-PCR.

    Techniques Used: Derivative Assay

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    The table shows the data on used primers: gene symbols, assay IDs, gene names, GenBank references sequence accession numbers andy amplicon lengths (bp).
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment"

    Article Title: The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-10187-y

    The table shows the data on used primers: gene symbols, assay IDs, gene names, GenBank references sequence accession numbers andy amplicon lengths (bp).
    Figure Legend Snippet: The table shows the data on used primers: gene symbols, assay IDs, gene names, GenBank references sequence accession numbers andy amplicon lengths (bp).

    Techniques Used: Sequencing, Amplification, Derivative Assay, Binding Assay

    The effects of novel adamantane derivatives (2, 3) and DPP4 inhibitors on Bdnf (A) and Cav1 (B) genes expression in the hippocampus isolated from diabetes mice. Bars in figure illustrate experimental groups— CTL non-diabetic mice, DM diabetic mice, DM-Vil vildagliptin-treated mice [20 mg/kg, po ], DM-Sax saxagliptin-treated mice [10 mg/kg, po ], DM-compound 2 compound 2-treated mice [50 mg/kg, po ], DM­compound 3 compound 3-treated mice [50 mg/kg, po ], respectively. Data is presented as mean ± SD (n = 8). One-way ANOVA, followed by Tukey's post hoc test was used. *p < 0.05 in comparison with control group; # p < 0.05 in comparison with diabetes group.
    Figure Legend Snippet: The effects of novel adamantane derivatives (2, 3) and DPP4 inhibitors on Bdnf (A) and Cav1 (B) genes expression in the hippocampus isolated from diabetes mice. Bars in figure illustrate experimental groups— CTL non-diabetic mice, DM diabetic mice, DM-Vil vildagliptin-treated mice [20 mg/kg, po ], DM-Sax saxagliptin-treated mice [10 mg/kg, po ], DM-compound 2 compound 2-treated mice [50 mg/kg, po ], DM­compound 3 compound 3-treated mice [50 mg/kg, po ], respectively. Data is presented as mean ± SD (n = 8). One-way ANOVA, followed by Tukey's post hoc test was used. *p < 0.05 in comparison with control group; # p < 0.05 in comparison with diabetes group.

    Techniques Used: Expressing, Isolation

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    The table shows the data on used primers: Gene symbols, assay IDs, gene names, GenBank reference sequence accession numbers and amplicon lengths (bp).
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp bdnf mm01334047 m1/product/Thermo Fisher
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    gene exp bdnf mm01334047 m1 - by Bioz Stars, 2023-03
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    Images

    1) Product Images from "The Role of Molecular and Inflammatory Indicators in the Assessment of Cognitive Dysfunction in a Mouse Model of Diabetes"

    Article Title: The Role of Molecular and Inflammatory Indicators in the Assessment of Cognitive Dysfunction in a Mouse Model of Diabetes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22083878

    The table shows the data on used primers: Gene symbols, assay IDs, gene names, GenBank reference sequence accession numbers and amplicon lengths (bp).
    Figure Legend Snippet: The table shows the data on used primers: Gene symbols, assay IDs, gene names, GenBank reference sequence accession numbers and amplicon lengths (bp).

    Techniques Used: Sequencing, Amplification, Activity Assay, Derivative Assay, Binding Assay

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp bdnf mm01334047 m1/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
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    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    Two weeks of exercise training (15 m/min for 60 min per day, five days per week) increases mRNA levels of ( A ) Nr5a1 (SF-1), ( B ) <t>Bdnf</t> , ( C ) Cnr1 , and ( D ) Crhr2 in the mediobasal hypothalamus of C57BL/6J mice. The mediobasal hypothalamus was collected 2 hr after the last bout of exercise training. Values are mean ± S.E.M. **p<0.01, ***p<0.001 DOI: http://dx.doi.org/10.7554/eLife.18206.003
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "SF-1 expression in the hypothalamus is required for beneficial metabolic effects of exercise"

    Article Title: SF-1 expression in the hypothalamus is required for beneficial metabolic effects of exercise

    Journal: eLife

    doi: 10.7554/eLife.18206

    Two weeks of exercise training (15 m/min for 60 min per day, five days per week) increases mRNA levels of ( A ) Nr5a1 (SF-1), ( B ) Bdnf , ( C ) Cnr1 , and ( D ) Crhr2 in the mediobasal hypothalamus of C57BL/6J mice. The mediobasal hypothalamus was collected 2 hr after the last bout of exercise training. Values are mean ± S.E.M. **p<0.01, ***p<0.001 DOI: http://dx.doi.org/10.7554/eLife.18206.003
    Figure Legend Snippet: Two weeks of exercise training (15 m/min for 60 min per day, five days per week) increases mRNA levels of ( A ) Nr5a1 (SF-1), ( B ) Bdnf , ( C ) Cnr1 , and ( D ) Crhr2 in the mediobasal hypothalamus of C57BL/6J mice. The mediobasal hypothalamus was collected 2 hr after the last bout of exercise training. Values are mean ± S.E.M. **p<0.01, ***p<0.001 DOI: http://dx.doi.org/10.7554/eLife.18206.003

    Techniques Used:

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    Primers with references used in real time quantitative PCR experiments m is mouse; r is rat; and h is human.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Brain-derived Neurotrophic Factor in Megakaryocytes"

    Article Title: Brain-derived Neurotrophic Factor in Megakaryocytes

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.720029

    Primers with references used in real time quantitative PCR experiments m is mouse; r is rat; and h is human.
    Figure Legend Snippet: Primers with references used in real time quantitative PCR experiments m is mouse; r is rat; and h is human.

    Techniques Used: Real-time Polymerase Chain Reaction, Sequencing

    Differential BDNF protein levels in mouse, rat, and human megakaryocytes and platelets. Western blot lysates of cultured Mks ( A ) and blood platelets ( B ) are shown. Eighty micrograms of protein per lane were loaded, and the blotting membrane was incubated with the mouse monoclonal antibody 3C11 developed by Icosagen (Tartu, Estonia). Recombinant BDNF and pro-BDNF were used as molecular mass markers and antibodies to β-actin as loading controls. Asterisks ( top right panels ) point to a band unrelated to BDNF likely corresponding to immunoglobulin light chains in the mouse sample. Note the absence of BDNF in mouse Mks and platelets. C, antibodies to BDNF 9 ( green ) and PF4 ( red ) reveal expression of both antigens in mature rat and human Mks. Note that unlike PF4, BDNF is not detectable in mouse Mks. The co-localization of BDNF with PF4 in rat and human Mks was quantified using the pixel intensity specifically generated by each channel. In humans, 83% and in rats 86% BDNF-positive granules were also PF4-positive. Blue , DAPI staining. D, immunofluorescence staining of F-actin ( red ) and BDNF ( green ) in proplatelet-forming cultured human Mks. Arrows indicate BDNF accumulation in proplatelet buds.
    Figure Legend Snippet: Differential BDNF protein levels in mouse, rat, and human megakaryocytes and platelets. Western blot lysates of cultured Mks ( A ) and blood platelets ( B ) are shown. Eighty micrograms of protein per lane were loaded, and the blotting membrane was incubated with the mouse monoclonal antibody 3C11 developed by Icosagen (Tartu, Estonia). Recombinant BDNF and pro-BDNF were used as molecular mass markers and antibodies to β-actin as loading controls. Asterisks ( top right panels ) point to a band unrelated to BDNF likely corresponding to immunoglobulin light chains in the mouse sample. Note the absence of BDNF in mouse Mks and platelets. C, antibodies to BDNF 9 ( green ) and PF4 ( red ) reveal expression of both antigens in mature rat and human Mks. Note that unlike PF4, BDNF is not detectable in mouse Mks. The co-localization of BDNF with PF4 in rat and human Mks was quantified using the pixel intensity specifically generated by each channel. In humans, 83% and in rats 86% BDNF-positive granules were also PF4-positive. Blue , DAPI staining. D, immunofluorescence staining of F-actin ( red ) and BDNF ( green ) in proplatelet-forming cultured human Mks. Arrows indicate BDNF accumulation in proplatelet buds.

    Techniques Used: Western Blot, Cell Culture, Incubation, Recombinant, Expressing, Generated, Staining, Immunofluorescence

    Transcriptional analysis of BDNF in mouse, rat, and human megakaryocytes. Conventional ( A ) and real time quantitative ( B ) PCR using exon-specific primers with RNA extracted from mature cultured Mks, adult hippocampus ( Hippo ), and lung are shown. Note that in the mouse, the neuron-specific transcripts, including exon I and IV, are not detected and that by contrast the transcript pattern resembles the non-neuronal pattern observed in lung tissue. The converse is the case with RNA extracted from rat and human Mks with transcript patterns, including exon I and IV, that are characteristic of a neuronal pattern as illustrated with the hippocampus. Unless indicated as non-significant ( n.s. ), all values are mean values ± S.E. in triplicates and based on three independent experiments, at p < 0.001 (paired t test).
    Figure Legend Snippet: Transcriptional analysis of BDNF in mouse, rat, and human megakaryocytes. Conventional ( A ) and real time quantitative ( B ) PCR using exon-specific primers with RNA extracted from mature cultured Mks, adult hippocampus ( Hippo ), and lung are shown. Note that in the mouse, the neuron-specific transcripts, including exon I and IV, are not detected and that by contrast the transcript pattern resembles the non-neuronal pattern observed in lung tissue. The converse is the case with RNA extracted from rat and human Mks with transcript patterns, including exon I and IV, that are characteristic of a neuronal pattern as illustrated with the hippocampus. Unless indicated as non-significant ( n.s. ), all values are mean values ± S.E. in triplicates and based on three independent experiments, at p < 0.001 (paired t test).

    Techniques Used: Cell Culture

    Up-regulation of Bdnf mRNA by thapsigargin. Effect of extracellular calcium. Dose response ( A ) and time course ( B ) of Bdnf mRNA expression by rat Mks after thapsigargin treatment. Purified mature rat Mks were cultured in the presence or absence of thapsigargin or vehicle (DMSO) used at the indicated concentrations ( A ) and for different lengths of times ( B ). Total mRNA was extracted and reverse-transcribed, and the resulting cDNA was amplified by real time quantitative PCR using specific primers for the coding sequence of Bdnf. C, extracellular calcium dependence of thapsigargin-induced Bdnf mRNA increase. Rat Mks were preincubated with 2.5 m m EGTA for 1.5 h at 37 °C followed by 10 n m thapsigargin for 4 h. mRNA expression was analyzed by real time quantitative PCR using specific primers for the coding sequence of Bdnf ( CDS ) or exon-specific primers. All values are mean values ± S.E. in triplicates and based on three independent experiments. Unless indicated, all the statistical values are compared with the control. *, p < 0.05; ***, p < 0.001 (paired t test).
    Figure Legend Snippet: Up-regulation of Bdnf mRNA by thapsigargin. Effect of extracellular calcium. Dose response ( A ) and time course ( B ) of Bdnf mRNA expression by rat Mks after thapsigargin treatment. Purified mature rat Mks were cultured in the presence or absence of thapsigargin or vehicle (DMSO) used at the indicated concentrations ( A ) and for different lengths of times ( B ). Total mRNA was extracted and reverse-transcribed, and the resulting cDNA was amplified by real time quantitative PCR using specific primers for the coding sequence of Bdnf. C, extracellular calcium dependence of thapsigargin-induced Bdnf mRNA increase. Rat Mks were preincubated with 2.5 m m EGTA for 1.5 h at 37 °C followed by 10 n m thapsigargin for 4 h. mRNA expression was analyzed by real time quantitative PCR using specific primers for the coding sequence of Bdnf ( CDS ) or exon-specific primers. All values are mean values ± S.E. in triplicates and based on three independent experiments. Unless indicated, all the statistical values are compared with the control. *, p < 0.05; ***, p < 0.001 (paired t test).

    Techniques Used: Expressing, Purification, Cell Culture, Amplification, Real-time Polymerase Chain Reaction, Sequencing

    Effect of thapsigargin on pro-BDNF, mature BDNF, and pro-peptide in rat Mks. Dose response ( A ) and time course ( B ) of pro-BDNF and mature BDNF proteins by rat Mks after thapsigargin treatment are shown. Mature Mks were cultured for 16 h at the indicated doses of thapsigargin ( A ) or 10 n m thapsigargin for the indicated times ( B ). Forty micrograms of protein per lane were loaded, and the blotting membrane was incubated with the mouse monoclonal antibody 3C11 developed by Icosagen. Arrows indicate intermediate proteolytic products of pro-BDNF ( C ). Time course of pro-BDNF and pro-peptide proteins generated by rat Mks incubated with 10 n m thapsigargin for the indicated time periods. Eighty micrograms protein per lane were loaded, and the blotting membrane was incubated with the mouse monoclonal antibody H1001G developed by GeneCopeia, Inc. The blots shown are representative of three independent experiments with similar results. Graphs show mean ± S.E. of the densitometric values quantified from the blots of the three separate experiments. ***, p < 0.001 (paired t test compared the corresponding controls). Recombinant BDNF (150–300 pg), cleavage-resistant recombinant pro-BDNF (0.5–1 ng), and recombinant pro-peptide (1–10 ng) were used as molecular mass markers and antibodies to β-actin as loading controls.
    Figure Legend Snippet: Effect of thapsigargin on pro-BDNF, mature BDNF, and pro-peptide in rat Mks. Dose response ( A ) and time course ( B ) of pro-BDNF and mature BDNF proteins by rat Mks after thapsigargin treatment are shown. Mature Mks were cultured for 16 h at the indicated doses of thapsigargin ( A ) or 10 n m thapsigargin for the indicated times ( B ). Forty micrograms of protein per lane were loaded, and the blotting membrane was incubated with the mouse monoclonal antibody 3C11 developed by Icosagen. Arrows indicate intermediate proteolytic products of pro-BDNF ( C ). Time course of pro-BDNF and pro-peptide proteins generated by rat Mks incubated with 10 n m thapsigargin for the indicated time periods. Eighty micrograms protein per lane were loaded, and the blotting membrane was incubated with the mouse monoclonal antibody H1001G developed by GeneCopeia, Inc. The blots shown are representative of three independent experiments with similar results. Graphs show mean ± S.E. of the densitometric values quantified from the blots of the three separate experiments. ***, p < 0.001 (paired t test compared the corresponding controls). Recombinant BDNF (150–300 pg), cleavage-resistant recombinant pro-BDNF (0.5–1 ng), and recombinant pro-peptide (1–10 ng) were used as molecular mass markers and antibodies to β-actin as loading controls.

    Techniques Used: Cell Culture, Incubation, Generated, Recombinant

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Thermo Fisher gene exp bdnf mm01334047 m1
    (A) Neither treatment nor exercise altered <t>BDNF</t> mRNA in the hippocampi of female mice. (B) AAS treatment significantly decreased BDNF mRNA in the hippocampi of male mice (Oil versus AAS; *p = 0.049). Exercise significantly increased BDNF in the hippocampi of oil-injected (Oil versus Oil+Ex; #p = 0.035) and AAS-treated (AAS versus AAS+Ex; ###p < 0.0001) male mice. In both sexes, n = 16 per group.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 1 article reviews
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    88/100 stars

    Images

    1) Product Images from "Sex and Exercise Interact to Alter the Expression of Anabolic Androgenic Steroid-Induced Anxiety-Like Behaviors in the Mouse"

    Article Title: Sex and Exercise Interact to Alter the Expression of Anabolic Androgenic Steroid-Induced Anxiety-Like Behaviors in the Mouse

    Journal: Hormones and behavior

    doi: 10.1016/j.yhbeh.2014.04.008

    (A) Neither treatment nor exercise altered BDNF mRNA in the hippocampi of female mice. (B) AAS treatment significantly decreased BDNF mRNA in the hippocampi of male mice (Oil versus AAS; *p = 0.049). Exercise significantly increased BDNF in the hippocampi of oil-injected (Oil versus Oil+Ex; #p = 0.035) and AAS-treated (AAS versus AAS+Ex; ###p < 0.0001) male mice. In both sexes, n = 16 per group.
    Figure Legend Snippet: (A) Neither treatment nor exercise altered BDNF mRNA in the hippocampi of female mice. (B) AAS treatment significantly decreased BDNF mRNA in the hippocampi of male mice (Oil versus AAS; *p = 0.049). Exercise significantly increased BDNF in the hippocampi of oil-injected (Oil versus Oil+Ex; #p = 0.035) and AAS-treated (AAS versus AAS+Ex; ###p < 0.0001) male mice. In both sexes, n = 16 per group.

    Techniques Used: Injection

    gene exp bdnf mm01334047 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp bdnf mm01334047 m1
    (A) Neither treatment nor exercise altered <t>BDNF</t> mRNA in the hippocampi of female mice. (B) AAS treatment significantly decreased BDNF mRNA in the hippocampi of male mice (Oil versus AAS; *p = 0.049). Exercise significantly increased BDNF in the hippocampi of oil-injected (Oil versus Oil+Ex; #p = 0.035) and AAS-treated (AAS versus AAS+Ex; ###p < 0.0001) male mice. In both sexes, n = 16 per group.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp bdnf mm01334047 m1/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp bdnf mm01334047 m1 - by Bioz Stars, 2023-03
    88/100 stars

    Images

    1) Product Images from "Sex and Exercise Interact to Alter the Expression of Anabolic Androgenic Steroid-Induced Anxiety-Like Behaviors in the Mouse"

    Article Title: Sex and Exercise Interact to Alter the Expression of Anabolic Androgenic Steroid-Induced Anxiety-Like Behaviors in the Mouse

    Journal: Hormones and behavior

    doi: 10.1016/j.yhbeh.2014.04.008

    (A) Neither treatment nor exercise altered BDNF mRNA in the hippocampi of female mice. (B) AAS treatment significantly decreased BDNF mRNA in the hippocampi of male mice (Oil versus AAS; *p = 0.049). Exercise significantly increased BDNF in the hippocampi of oil-injected (Oil versus Oil+Ex; #p = 0.035) and AAS-treated (AAS versus AAS+Ex; ###p < 0.0001) male mice. In both sexes, n = 16 per group.
    Figure Legend Snippet: (A) Neither treatment nor exercise altered BDNF mRNA in the hippocampi of female mice. (B) AAS treatment significantly decreased BDNF mRNA in the hippocampi of male mice (Oil versus AAS; *p = 0.049). Exercise significantly increased BDNF in the hippocampi of oil-injected (Oil versus Oil+Ex; #p = 0.035) and AAS-treated (AAS versus AAS+Ex; ###p < 0.0001) male mice. In both sexes, n = 16 per group.

    Techniques Used: Injection

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    Thermo Fisher gene exp bdnf mm01334047 m1
    A–C Relative amounts of mRNA for NGF, <t>BDNF</t> and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.
    Gene Exp Bdnf Mm01334047 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp bdnf mm01334047 m1/product/Thermo Fisher
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    A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.

    Journal: PLoS ONE

    Article Title: Environmental Enrichment Extends Photoreceptor Survival and Visual Function in a Mouse Model of Retinitis Pigmentosa

    doi: 10.1371/journal.pone.0050726

    Figure Lengend Snippet: A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.

    Article Snippet: The probes used for quantitative real-time PCR were all TaqMan® Gene Expression Assays (20×) as reported below: GADPH: Mm99999915_g1*; NGF: Mn00443039_m1; BDNF: Mm01334047_m1; mTOR: Mm00444968_m1; CNTF: Mm00446373_m1.

    Techniques: Quantitative RT-PCR