Structured Review

Geneaid Biotech Ltd gel pcr dna fragments extraction kit
Host and viral <t>DNA</t> content of differently treated ASFV samples. Quantitative dual <t>PCR</t> was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
Gel Pcr Dna Fragments Extraction Kit, supplied by Geneaid Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel pcr dna fragments extraction kit/product/Geneaid Biotech Ltd
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gel pcr dna fragments extraction kit - by Bioz Stars, 2021-03
86/100 stars

Images

1) Product Images from "A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus"

Article Title: A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus

Journal: Viruses

doi: 10.3390/v11121129

Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
Figure Legend Snippet: Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

Techniques Used: Polymerase Chain Reaction, Whole Genome Amplification

2) Product Images from "A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus"

Article Title: A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus

Journal: Viruses

doi: 10.3390/v11121129

Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
Figure Legend Snippet: Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

Techniques Used: Polymerase Chain Reaction, Whole Genome Amplification

3) Product Images from "A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus"

Article Title: A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus

Journal: Viruses

doi: 10.3390/v11121129

Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
Figure Legend Snippet: Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

Techniques Used: Polymerase Chain Reaction, Whole Genome Amplification

4) Product Images from "First isolation and characterization of Brucella microti from wild boar"

Article Title: First isolation and characterization of Brucella microti from wild boar

Journal: BMC Veterinary Research

doi: 10.1186/s12917-015-0456-z

Results of upgraded “Bruce- and Suis-ladder” PCRs of HUN-Bmi-01 strain. left side Bruce-ladder v2.0 PCR with 1682, 1071, 774, 587, 510, 450, 272, and 152 bp amplicons. M: 100 bp DNA marker, S: B. suis , C: B. canis , O: B. ovis , A: B. abortus . right side Suis-ladder PCR with 774 and 197 bp amplicons. M: 100 bp DNA marker, S1: B. suis biovar. 1, S2: B. suis biovar. 2
Figure Legend Snippet: Results of upgraded “Bruce- and Suis-ladder” PCRs of HUN-Bmi-01 strain. left side Bruce-ladder v2.0 PCR with 1682, 1071, 774, 587, 510, 450, 272, and 152 bp amplicons. M: 100 bp DNA marker, S: B. suis , C: B. canis , O: B. ovis , A: B. abortus . right side Suis-ladder PCR with 774 and 197 bp amplicons. M: 100 bp DNA marker, S1: B. suis biovar. 1, S2: B. suis biovar. 2

Techniques Used: Polymerase Chain Reaction, Marker

Related Articles

Amplification:

Article Title: Screening of the LTBP2 gene in a north Indian population with primary congenital glaucoma
Article Snippet: Agarose gel was analyzed using a gel documentation system (Applied Biosystems, Carlsbad, CA). .. Successfully amplified PCR products were purified using a gel/PCR DNA fragments extraction kit (Catalog number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). .. Purified PCR products were sent for sequencing to MCLAB (Molecular Cloning Laboratories, South San Francisco, CA).

Article Title: First isolation and characterization of Brucella microti from wild boar
Article Snippet: Products between 300 and 350 bp were directly used without further purification in the PCR mixture of the NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ kit (New England Biolabs). .. Library amplification involved 12 amplification and the products were purified by the Gel/PCR DNA Fragments Extraction kit (Geneaid). .. The DNA was eluted in 50 μl nuclease free water and quantified fluorometrically on Qubit® 2.0 equipment using the Qubit® dsDNA BR Assay kit (Invitrogen).

Article Title: Potential impact on kidney infection: a whole-genome analysis of Leptospirasantarosai serovar Shermani
Article Snippet: The PCR products were separated on 1% agarose gels containing 0.05% ethidium bromide and visualized under ultraviolet light, sized and photographed (ChemiDoc XRS system; Bio-Rad, Hercules, CA, USA). .. The amplification products were recovered and purified with a Gel/PCR DNA Fragments Extraction Kit (Geneaid Biotech. .. Ltd) and the sequencing of the resulting PCR products was performed by the DNA Sequencing Core Laboratory (Chang Gung Memorial Hospital, Linkou, Taiwan).

Article Title: PAX6 gene analysis in irido-fundal coloboma
Article Snippet: Agarose gels were analyzed using the Gel Documentation System (Applied Biosystems, Carlsbad, CA). .. Amplified PCR products were purified using gel/PCR DNA fragments extraction kit (Catalog number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). ..

Article Title: VSX1 gene analysis in keratoconus
Article Snippet: Agarose gels were analyzed using gel documentation system (Applied Biosystems, Carlsbad, CA). .. Amplified PCR products were purified using gel/PCR DNA fragments extraction kit (DF100; Geneaid Biotech Ltd., Sijhih City Taiwan). .. The purified PCR products were sent for sequencing at MCLAB (Molecular Cloning Laboratories, South San Francisco, CA) Nucleotide sequences were compared with the VSX1 ensembl reference sequence.

Article Title: Mutations Associated with Decreased Susceptibility to Seven Antimicrobial Families in Field and Laboratory-Derived Mycoplasma bovis Strains
Article Snippet: Products between 300 and 350 bp were directly used without further purification in the PCR mixture of the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit (New England BioLabs). .. Library amplification was performed in 12 cycles, and the products were purified by the Gel/PCR DNA fragments extraction kit (Geneaid). .. The library DNA was eluted in 50 μl of nuclease-free water and quantified fluorometrically on Qubit 2.0 equipment using a Qubit dsDNA BR assay kit (Invitrogen).

Article Title: Axenfeld-Rieger Syndrome Associated with Congenital Glaucoma and Cytochrome P4501B1 Gene Mutations
Article Snippet: PCR amplifications for PITX2 all primer sets were performed in a 40 μ L volume containing 1.0 μ L of 20 μ M stock solution for each primer, 100 ng of genomic DNA, 1 unit of Taq polymerase (Banglore Genei), 0.1 mM of each dNTP, and 4 μ L of 10X PCR buffer (with 15 mM MgCl2 ), by means of 40 cycles of amplification, each consisting of 30 seconds denaturation at 94°C, 50 seconds annealing at 56–57°C, and 50 seconds extension at 72°C and final extension at 72°C for 5 minutes. .. Amplified PCR products were purified using a gel/PCR DNA fragments extraction kit (number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). .. Purified PCR products were sent for sequencing to MCLAB (Molecular Cloning Laboratories, South San Francisco, CA).

Polymerase Chain Reaction:

Article Title: Screening of the LTBP2 gene in a north Indian population with primary congenital glaucoma
Article Snippet: Agarose gel was analyzed using a gel documentation system (Applied Biosystems, Carlsbad, CA). .. Successfully amplified PCR products were purified using a gel/PCR DNA fragments extraction kit (Catalog number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). .. Purified PCR products were sent for sequencing to MCLAB (Molecular Cloning Laboratories, South San Francisco, CA).

Article Title: PAX6 gene analysis in irido-fundal coloboma
Article Snippet: Agarose gels were analyzed using the Gel Documentation System (Applied Biosystems, Carlsbad, CA). .. Amplified PCR products were purified using gel/PCR DNA fragments extraction kit (Catalog number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). ..

Article Title: VSX1 gene analysis in keratoconus
Article Snippet: Agarose gels were analyzed using gel documentation system (Applied Biosystems, Carlsbad, CA). .. Amplified PCR products were purified using gel/PCR DNA fragments extraction kit (DF100; Geneaid Biotech Ltd., Sijhih City Taiwan). .. The purified PCR products were sent for sequencing at MCLAB (Molecular Cloning Laboratories, South San Francisco, CA) Nucleotide sequences were compared with the VSX1 ensembl reference sequence.

Article Title: Axenfeld-Rieger Syndrome Associated with Congenital Glaucoma and Cytochrome P4501B1 Gene Mutations
Article Snippet: PCR amplifications for PITX2 all primer sets were performed in a 40 μ L volume containing 1.0 μ L of 20 μ M stock solution for each primer, 100 ng of genomic DNA, 1 unit of Taq polymerase (Banglore Genei), 0.1 mM of each dNTP, and 4 μ L of 10X PCR buffer (with 15 mM MgCl2 ), by means of 40 cycles of amplification, each consisting of 30 seconds denaturation at 94°C, 50 seconds annealing at 56–57°C, and 50 seconds extension at 72°C and final extension at 72°C for 5 minutes. .. Amplified PCR products were purified using a gel/PCR DNA fragments extraction kit (number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). .. Purified PCR products were sent for sequencing to MCLAB (Molecular Cloning Laboratories, South San Francisco, CA).

Purification:

Article Title: Screening of the LTBP2 gene in a north Indian population with primary congenital glaucoma
Article Snippet: Agarose gel was analyzed using a gel documentation system (Applied Biosystems, Carlsbad, CA). .. Successfully amplified PCR products were purified using a gel/PCR DNA fragments extraction kit (Catalog number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). .. Purified PCR products were sent for sequencing to MCLAB (Molecular Cloning Laboratories, South San Francisco, CA).

Article Title: First isolation and characterization of Brucella microti from wild boar
Article Snippet: Products between 300 and 350 bp were directly used without further purification in the PCR mixture of the NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ kit (New England Biolabs). .. Library amplification involved 12 amplification and the products were purified by the Gel/PCR DNA Fragments Extraction kit (Geneaid). .. The DNA was eluted in 50 μl nuclease free water and quantified fluorometrically on Qubit® 2.0 equipment using the Qubit® dsDNA BR Assay kit (Invitrogen).

Article Title: Potential impact on kidney infection: a whole-genome analysis of Leptospirasantarosai serovar Shermani
Article Snippet: The PCR products were separated on 1% agarose gels containing 0.05% ethidium bromide and visualized under ultraviolet light, sized and photographed (ChemiDoc XRS system; Bio-Rad, Hercules, CA, USA). .. The amplification products were recovered and purified with a Gel/PCR DNA Fragments Extraction Kit (Geneaid Biotech. .. Ltd) and the sequencing of the resulting PCR products was performed by the DNA Sequencing Core Laboratory (Chang Gung Memorial Hospital, Linkou, Taiwan).

Article Title: PAX6 gene analysis in irido-fundal coloboma
Article Snippet: Agarose gels were analyzed using the Gel Documentation System (Applied Biosystems, Carlsbad, CA). .. Amplified PCR products were purified using gel/PCR DNA fragments extraction kit (Catalog number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). ..

Article Title: VSX1 gene analysis in keratoconus
Article Snippet: Agarose gels were analyzed using gel documentation system (Applied Biosystems, Carlsbad, CA). .. Amplified PCR products were purified using gel/PCR DNA fragments extraction kit (DF100; Geneaid Biotech Ltd., Sijhih City Taiwan). .. The purified PCR products were sent for sequencing at MCLAB (Molecular Cloning Laboratories, South San Francisco, CA) Nucleotide sequences were compared with the VSX1 ensembl reference sequence.

Article Title: Mutations Associated with Decreased Susceptibility to Seven Antimicrobial Families in Field and Laboratory-Derived Mycoplasma bovis Strains
Article Snippet: Products between 300 and 350 bp were directly used without further purification in the PCR mixture of the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit (New England BioLabs). .. Library amplification was performed in 12 cycles, and the products were purified by the Gel/PCR DNA fragments extraction kit (Geneaid). .. The library DNA was eluted in 50 μl of nuclease-free water and quantified fluorometrically on Qubit 2.0 equipment using a Qubit dsDNA BR assay kit (Invitrogen).

Article Title: Axenfeld-Rieger Syndrome Associated with Congenital Glaucoma and Cytochrome P4501B1 Gene Mutations
Article Snippet: PCR amplifications for PITX2 all primer sets were performed in a 40 μ L volume containing 1.0 μ L of 20 μ M stock solution for each primer, 100 ng of genomic DNA, 1 unit of Taq polymerase (Banglore Genei), 0.1 mM of each dNTP, and 4 μ L of 10X PCR buffer (with 15 mM MgCl2 ), by means of 40 cycles of amplification, each consisting of 30 seconds denaturation at 94°C, 50 seconds annealing at 56–57°C, and 50 seconds extension at 72°C and final extension at 72°C for 5 minutes. .. Amplified PCR products were purified using a gel/PCR DNA fragments extraction kit (number DF100; Geneaid Biotech Ltd., Sijhih City, Taiwan). .. Purified PCR products were sent for sequencing to MCLAB (Molecular Cloning Laboratories, South San Francisco, CA).

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  • 86
    Geneaid Biotech Ltd gel pcr dna fragments kit
    Results of <t>PCR</t> fingerprinting regarding false detection. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus <t>DNA</t> ladder, lane 1: unique amplicons (550 bp, 650 bp) in Holstein by primer OPK19 (shown in arrow mark), lane 2 Angus with primer OPK19, lane 3: Taiwan Yellow Cattle with primer OPK19
    Gel Pcr Dna Fragments Kit, supplied by Geneaid Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel pcr dna fragments kit/product/Geneaid Biotech Ltd
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel pcr dna fragments kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Geneaid Biotech Ltd gel pcr dna fragments extraction kit
    Host and viral <t>DNA</t> content of differently treated ASFV samples. Quantitative dual <t>PCR</t> was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
    Gel Pcr Dna Fragments Extraction Kit, supplied by Geneaid Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel pcr dna fragments extraction kit/product/Geneaid Biotech Ltd
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel pcr dna fragments extraction kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Results of PCR fingerprinting regarding false detection. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus DNA ladder, lane 1: unique amplicons (550 bp, 650 bp) in Holstein by primer OPK19 (shown in arrow mark), lane 2 Angus with primer OPK19, lane 3: Taiwan Yellow Cattle with primer OPK19

    Journal: Food Science and Biotechnology

    Article Title: Development of RAPD-PCR assay for identifying Holstein, Angus, and Taiwan Yellow Cattle for meat adulteration detection

    doi: 10.1007/s10068-019-00607-7

    Figure Lengend Snippet: Results of PCR fingerprinting regarding false detection. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus DNA ladder, lane 1: unique amplicons (550 bp, 650 bp) in Holstein by primer OPK19 (shown in arrow mark), lane 2 Angus with primer OPK19, lane 3: Taiwan Yellow Cattle with primer OPK19

    Article Snippet: The RAPD-PCR was processed on a Bio-Rad T100™ thermal cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA), and the cycling program was as follows: 94 °C for 5 min, followed by 45 cycles at 94 °C for 1 min, 36 °C for 1 min and 72 °C for 1 min with a final extension of 72 °C for 10 min. A scalpel was used for excising gel fragment, and the amplified product was extracted using the Gel/PCR DNA Fragments Kit (Geneaid Biotech Ltd. New Taipei City, Taiwan).

    Techniques: Polymerase Chain Reaction

    A RAPD-PCR amplification of pattern showing distinction between sub-species. M: 100 bp plus DNA ladder, lane 1: primer OPK07 and Holstein DNA, lane 2: primer OPK07 and Angus DNA, showing a unique amplicon with the molecular weight of 1200 bp (shown in arrow mark), lane 3: primer OPK07 and Taiwan Yellow Cattle DNA. B RAPD-PCR amplification of pattern showing distinction between sub-species. M: 100 bp plus DNA ladder, lane 1: primer OPK12 and Holstein DNA, lane 2: primer OPK12 and Angus DNA, showing a unique amplicon with the molecular weight of 700 bp (shown in arrow mark), lane3: primer OPK12 and Taiwan Yellow Cattle DNA, lane 4: primer OPK14 and Holstein DNA, lane 5: primer OPK14 and Angus DNA, showing a unique amplicon with the molecular weight of 1800 bp (shown in arrow mark), lane 6: primer OPK14 and Taiwan Yellow Cattle DNA, lane 7: primer OPK19 and Holstein DNA, lane 8: primer OPK19 and Angus DNA, showing a unique amplicon with the molecular weight of 600 bp (shown in arrow mark), lane 9: primer OPK19 and Taiwan Yellow Cattle DNA

    Journal: Food Science and Biotechnology

    Article Title: Development of RAPD-PCR assay for identifying Holstein, Angus, and Taiwan Yellow Cattle for meat adulteration detection

    doi: 10.1007/s10068-019-00607-7

    Figure Lengend Snippet: A RAPD-PCR amplification of pattern showing distinction between sub-species. M: 100 bp plus DNA ladder, lane 1: primer OPK07 and Holstein DNA, lane 2: primer OPK07 and Angus DNA, showing a unique amplicon with the molecular weight of 1200 bp (shown in arrow mark), lane 3: primer OPK07 and Taiwan Yellow Cattle DNA. B RAPD-PCR amplification of pattern showing distinction between sub-species. M: 100 bp plus DNA ladder, lane 1: primer OPK12 and Holstein DNA, lane 2: primer OPK12 and Angus DNA, showing a unique amplicon with the molecular weight of 700 bp (shown in arrow mark), lane3: primer OPK12 and Taiwan Yellow Cattle DNA, lane 4: primer OPK14 and Holstein DNA, lane 5: primer OPK14 and Angus DNA, showing a unique amplicon with the molecular weight of 1800 bp (shown in arrow mark), lane 6: primer OPK14 and Taiwan Yellow Cattle DNA, lane 7: primer OPK19 and Holstein DNA, lane 8: primer OPK19 and Angus DNA, showing a unique amplicon with the molecular weight of 600 bp (shown in arrow mark), lane 9: primer OPK19 and Taiwan Yellow Cattle DNA

    Article Snippet: The RAPD-PCR was processed on a Bio-Rad T100™ thermal cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA), and the cycling program was as follows: 94 °C for 5 min, followed by 45 cycles at 94 °C for 1 min, 36 °C for 1 min and 72 °C for 1 min with a final extension of 72 °C for 10 min. A scalpel was used for excising gel fragment, and the amplified product was extracted using the Gel/PCR DNA Fragments Kit (Geneaid Biotech Ltd. New Taipei City, Taiwan).

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight

    Results of PCR fingerprinting regarding false detection. Amplicon showed only in Holstein and Angus. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus DNA ladder, lane 1: amplicon with 180 bp molecular weights in Holstein by primer OPK14 (shown in arrow mark), lane 2: amplicon with 180 bp molecular weights in Angus by primer OPK14 (shown in arrow mark), lane 3: Taiwan Yellow Cattle with primer OPK14

    Journal: Food Science and Biotechnology

    Article Title: Development of RAPD-PCR assay for identifying Holstein, Angus, and Taiwan Yellow Cattle for meat adulteration detection

    doi: 10.1007/s10068-019-00607-7

    Figure Lengend Snippet: Results of PCR fingerprinting regarding false detection. Amplicon showed only in Holstein and Angus. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus DNA ladder, lane 1: amplicon with 180 bp molecular weights in Holstein by primer OPK14 (shown in arrow mark), lane 2: amplicon with 180 bp molecular weights in Angus by primer OPK14 (shown in arrow mark), lane 3: Taiwan Yellow Cattle with primer OPK14

    Article Snippet: The RAPD-PCR was processed on a Bio-Rad T100™ thermal cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA), and the cycling program was as follows: 94 °C for 5 min, followed by 45 cycles at 94 °C for 1 min, 36 °C for 1 min and 72 °C for 1 min with a final extension of 72 °C for 10 min. A scalpel was used for excising gel fragment, and the amplified product was extracted using the Gel/PCR DNA Fragments Kit (Geneaid Biotech Ltd. New Taipei City, Taiwan).

    Techniques: Polymerase Chain Reaction, Amplification

    Results of PCR fingerprinting of false detection. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus DNA ladder, lane 1: unique amplicons (1000 bp, 1100 bp and 480 bp) in Holstein by primer OPK12 (shown in arrow mark), lane 2: Angus with primer OPK12, lane 3: Taiwan Yellow Cattle with primer OPK12

    Journal: Food Science and Biotechnology

    Article Title: Development of RAPD-PCR assay for identifying Holstein, Angus, and Taiwan Yellow Cattle for meat adulteration detection

    doi: 10.1007/s10068-019-00607-7

    Figure Lengend Snippet: Results of PCR fingerprinting of false detection. H: Holstein, A: Angus, Y: Taiwan Yellow Cattle, HAY: Holstein + Angus + Taiwan Yellow Cattle. M: 100 bp Plus DNA ladder, lane 1: unique amplicons (1000 bp, 1100 bp and 480 bp) in Holstein by primer OPK12 (shown in arrow mark), lane 2: Angus with primer OPK12, lane 3: Taiwan Yellow Cattle with primer OPK12

    Article Snippet: The RAPD-PCR was processed on a Bio-Rad T100™ thermal cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA), and the cycling program was as follows: 94 °C for 5 min, followed by 45 cycles at 94 °C for 1 min, 36 °C for 1 min and 72 °C for 1 min with a final extension of 72 °C for 10 min. A scalpel was used for excising gel fragment, and the amplified product was extracted using the Gel/PCR DNA Fragments Kit (Geneaid Biotech Ltd. New Taipei City, Taiwan).

    Techniques: Polymerase Chain Reaction

    Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

    Journal: Viruses

    Article Title: A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus

    doi: 10.3390/v11121129

    Figure Lengend Snippet: Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

    Article Snippet: The barcoded library DNA samples were purified using the Gel/PCR DNA fragments extraction kit (Geneaid Biotech, Ltd., Taipei, Taiwan) according to the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Whole Genome Amplification