gel filtration reference marker standards  (Bio-Rad)

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Illustration of topical delivery of adapalene from gel formulations. Arrows indicate the typical penetration pathway of adapalene through skin layers. Due to a high logP, adapalene tends to interact with the lipids in the skin (sebum and lipid matrix in SC).

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: LC–OCT horizontal view windows of baseline ( A ) and 48 h ( B ) after daily application of adapalene to healthy skin of same participant. Image taken approximately 60 um deep at level of stratum spinosum. Notable structures include infundibula (circular hypointense structures) with associated hair shaft (linear tubular structures extending from infundibula). Keratinocyte nuclei (small black circular to polygonal shapes) counted by cell profiler, in this patient ( A ) with 2002 keratinocytes and ( B ) with 3377 keratinocytes, while B exhibited increase count in this instance, the average of 3 images showed no statistically significant difference.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: LC–OCT horizontal view windows showing an example of a ( A ) baseline image with dilated infundibula filled with hyperrefractile keratin debris and ( B ) the same participant 48 h after daily adapalene application with an infundibula with normal size (not expanded beyond diameter of hair shaft). In addition, annotated images show how infundibular area was measured and calculated.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Graphical representation of release profiles of adapalene from the three marketed gels per cm 2 of membrane ( n = 6). Values below LOQ were considered as zero, to perform statistical analysis. Statistical significance: ** p < 0.01, **** p < 0.0001; ns = not significant.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques: Membrane

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Cumulative amount of adapalene per weight of epidermis (SC + viable epidermis) in a 36-h IVPT study using dermatomed human cadaver skin ( n = 6). Statistical significance: **** p < 0.0001; ns = not significant.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Cumulative amount of adapalene per weight of dermis in a 36-h IVPT study using dermatomed human cadaver skin (n = 6). Statistical significance: **** p < 0.0001; ns = not significant.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Experiments vs. in silico IVRT model predictions for Differin formulation.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques: In Silico, Formulation

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: LC–OCT measurable reduction in SC thickness at different time points following cheek application of either Differin ( p = 0.0035 at 48 h, n = 4), Effaclar ( p = 0.0187 at 48 h, n = 3), or Acne Free ( p < 0.0104 at 48 h, n = 3) adapalene formulations compared to baseline. Each point corresponds to data from a unique image acquisition and each color to a unique patient. ns = not significant, * p ≤ 0.05, ** p ≤ 0.01.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: LC–OCT measurable reduction in pore size (infundibular to isthmus ratio) at different time points following cheek application of either Differin ( p < 0.0001, n = 4), Effaclar ( p < 0.0001, n = 4), or Acne Free ( p < 0.0001, n = 4) adapalene formulations compared to baseline. Each point corresponds to data from a unique image acquisition and each color to a unique patient. ns = not significant, * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques: Pore Size

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Epidermal thickness measured by LC–OCT at different timepoints following cheek application of either Differin ( n = 4), Effaclar ( n = 4), or Acne Free ( n = 4) adapalene formulations compared to baseline. Each point corresponds to data from a unique image acquisition and each color to a unique patient. ns = not significant.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Keratinocyte area measured by LC–OCT at different timepoints following cheek application of either Differin ( n = 4), Effaclar ( n = 4), or Acne Free ( n = 4) adapalene formulations compared to baseline. Each point corresponds to data from a unique image acquisition and each color to a unique patient.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Keratinocyte count measured by LC–OCT at different time points following cheek application of either Differin ( n = 4), Effaclar ( n = 4), or Acne Free ( n = 4) adapalene formulations compared to baseline. Each point corresponds to data from a unique image acquisition and each color to a unique patient.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Observed decrease in the infundibular area over time for the three adapalene gel formulations. Dots represent individual observations and the solid lines represent geometric means of the formulations (green–Differin, black–AcneFree, pink–Effaclar). The gray dashed line shows the geometric mean of the pooled data.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Empirical Bayes estimates (EBEs) of individual Hill coefficients ( y -axis) compared across formulations ( x -axis) to investigate potential differences between the three gels (black—Acnefree, green—-Differin, pink—Effaclar). The solid lines indicate the median, and the dots indicate outliers.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Observed decrease in the SC thickness over time for the three adapalene gel formulations. Dots represent individual observations and the solid lines represent geometric means of the formulations (green—Differin, black—AcneFree, pink—Effaclar). The gray dashed line shows the geometric mean of the pooled data.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Empirical Bayes Estimates (EBEs) of individual Emax values ( y = -axis) compared across formulations to investigate potential differences between the three gels (black–Acnefree, green–Differin, pink–Effaclar). The solid lines indicate the median. Statistical significance: ** p < 0.01.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques:

Journal: Pharmaceutics

Article Title: A Mechanistic Physiologically Based Pharmacokinetic/Pharmacodynamic Modeling Approach Informed by In Vitro and Clinical Studies for Topical Administration of Adapalene Gels

doi: 10.3390/pharmaceutics17091108

Figure Lengend Snippet: Empirical Bayes Estimates (EBEs) of individual EC50 values ( y -axis) compared across formulations to investigate potential differences between the three gels (black–Acnefree, green–Differin, pink–Effaclar). The solid lines indicate the median. Statistical significance: *** represents p < 0.001.

Article Snippet: 0.5 oz; 15 g) by Dermatology Inspired Care (Lot No. YWS93W), Differin ® Gel (Reference) 0.1% by Galderma (Net wt.

Techniques: