gel filtration chromatography protein solutions  (GE Healthcare)

 
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    GE Healthcare gel filtration chromatography protein solutions
    <t>Gel-filtration</t> <t>chromatography</t> of Pmr and Pmr-R8A. (A) Elution profiles of Pmr (blue line) and Pmr-R8A (red line). The figures present the absorbance at 280 nm as a function of the elution volume (ml). One milliliter of purified proteins at 140 µM was applied to a HiLoad 16/60 Superdex 200 prep-grade column in buffer B (20 mM Tris-HCl (pH 7.5, 4°C), 0.5 M NaCl, 10% glycerol, and 0.5 M imidazole). The numbers indicate the fractions which were applied to Tricine-SDS-PAGE and Western blot analysis. (B) Tricine-SDS-PAGE profiles of fractions from gel filtration chromatography. The numbers correspond to elution profiles shown in panel A. Ten microliters were applied to Tricine-SDS-PAGE from 1 ml of the fraction. “M” indicates the <t>protein</t> marker. (C) Western blot analysis using anti-His antibody and the same samples in Tricine-SDS-PAGE shown in panel B.
    Gel Filtration Chromatography Protein Solutions, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel filtration chromatography protein solutions/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel filtration chromatography protein solutions - by Bioz Stars, 2020-08
    86/100 stars

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    1) Product Images from "Oligomerization Mechanisms of an H-NS Family Protein, Pmr, Encoded on the Plasmid pCAR1 Provide a Molecular Basis for Functions of H-NS Family Members"

    Article Title: Oligomerization Mechanisms of an H-NS Family Protein, Pmr, Encoded on the Plasmid pCAR1 Provide a Molecular Basis for Functions of H-NS Family Members

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0105656

    Gel-filtration chromatography of Pmr and Pmr-R8A. (A) Elution profiles of Pmr (blue line) and Pmr-R8A (red line). The figures present the absorbance at 280 nm as a function of the elution volume (ml). One milliliter of purified proteins at 140 µM was applied to a HiLoad 16/60 Superdex 200 prep-grade column in buffer B (20 mM Tris-HCl (pH 7.5, 4°C), 0.5 M NaCl, 10% glycerol, and 0.5 M imidazole). The numbers indicate the fractions which were applied to Tricine-SDS-PAGE and Western blot analysis. (B) Tricine-SDS-PAGE profiles of fractions from gel filtration chromatography. The numbers correspond to elution profiles shown in panel A. Ten microliters were applied to Tricine-SDS-PAGE from 1 ml of the fraction. “M” indicates the protein marker. (C) Western blot analysis using anti-His antibody and the same samples in Tricine-SDS-PAGE shown in panel B.
    Figure Legend Snippet: Gel-filtration chromatography of Pmr and Pmr-R8A. (A) Elution profiles of Pmr (blue line) and Pmr-R8A (red line). The figures present the absorbance at 280 nm as a function of the elution volume (ml). One milliliter of purified proteins at 140 µM was applied to a HiLoad 16/60 Superdex 200 prep-grade column in buffer B (20 mM Tris-HCl (pH 7.5, 4°C), 0.5 M NaCl, 10% glycerol, and 0.5 M imidazole). The numbers indicate the fractions which were applied to Tricine-SDS-PAGE and Western blot analysis. (B) Tricine-SDS-PAGE profiles of fractions from gel filtration chromatography. The numbers correspond to elution profiles shown in panel A. Ten microliters were applied to Tricine-SDS-PAGE from 1 ml of the fraction. “M” indicates the protein marker. (C) Western blot analysis using anti-His antibody and the same samples in Tricine-SDS-PAGE shown in panel B.

    Techniques Used: Filtration, Chromatography, Purification, SDS Page, Western Blot, Marker

    Related Articles

    Chromatography:

    Article Title: Oligomerization Mechanisms of an H-NS Family Protein, Pmr, Encoded on the Plasmid pCAR1 Provide a Molecular Basis for Functions of H-NS Family Members
    Article Snippet: .. Gel-filtration chromatography Protein solutions after purification using HiTrap Chelating HP column were applied to a HiLoad 16/60 Superdex 200 prep-grade column (GE Healthcare). ..

    Purification:

    Article Title: Oligomerization Mechanisms of an H-NS Family Protein, Pmr, Encoded on the Plasmid pCAR1 Provide a Molecular Basis for Functions of H-NS Family Members
    Article Snippet: .. Gel-filtration chromatography Protein solutions after purification using HiTrap Chelating HP column were applied to a HiLoad 16/60 Superdex 200 prep-grade column (GE Healthcare). ..

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    GE Healthcare gel filtration chromatography protein solutions
    <t>Gel-filtration</t> <t>chromatography</t> of Pmr and Pmr-R8A. (A) Elution profiles of Pmr (blue line) and Pmr-R8A (red line). The figures present the absorbance at 280 nm as a function of the elution volume (ml). One milliliter of purified proteins at 140 µM was applied to a HiLoad 16/60 Superdex 200 prep-grade column in buffer B (20 mM Tris-HCl (pH 7.5, 4°C), 0.5 M NaCl, 10% glycerol, and 0.5 M imidazole). The numbers indicate the fractions which were applied to Tricine-SDS-PAGE and Western blot analysis. (B) Tricine-SDS-PAGE profiles of fractions from gel filtration chromatography. The numbers correspond to elution profiles shown in panel A. Ten microliters were applied to Tricine-SDS-PAGE from 1 ml of the fraction. “M” indicates the <t>protein</t> marker. (C) Western blot analysis using anti-His antibody and the same samples in Tricine-SDS-PAGE shown in panel B.
    Gel Filtration Chromatography Protein Solutions, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel filtration chromatography protein solutions/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel filtration chromatography protein solutions - by Bioz Stars, 2020-08
    86/100 stars
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    Gel-filtration chromatography of Pmr and Pmr-R8A. (A) Elution profiles of Pmr (blue line) and Pmr-R8A (red line). The figures present the absorbance at 280 nm as a function of the elution volume (ml). One milliliter of purified proteins at 140 µM was applied to a HiLoad 16/60 Superdex 200 prep-grade column in buffer B (20 mM Tris-HCl (pH 7.5, 4°C), 0.5 M NaCl, 10% glycerol, and 0.5 M imidazole). The numbers indicate the fractions which were applied to Tricine-SDS-PAGE and Western blot analysis. (B) Tricine-SDS-PAGE profiles of fractions from gel filtration chromatography. The numbers correspond to elution profiles shown in panel A. Ten microliters were applied to Tricine-SDS-PAGE from 1 ml of the fraction. “M” indicates the protein marker. (C) Western blot analysis using anti-His antibody and the same samples in Tricine-SDS-PAGE shown in panel B.

    Journal: PLoS ONE

    Article Title: Oligomerization Mechanisms of an H-NS Family Protein, Pmr, Encoded on the Plasmid pCAR1 Provide a Molecular Basis for Functions of H-NS Family Members

    doi: 10.1371/journal.pone.0105656

    Figure Lengend Snippet: Gel-filtration chromatography of Pmr and Pmr-R8A. (A) Elution profiles of Pmr (blue line) and Pmr-R8A (red line). The figures present the absorbance at 280 nm as a function of the elution volume (ml). One milliliter of purified proteins at 140 µM was applied to a HiLoad 16/60 Superdex 200 prep-grade column in buffer B (20 mM Tris-HCl (pH 7.5, 4°C), 0.5 M NaCl, 10% glycerol, and 0.5 M imidazole). The numbers indicate the fractions which were applied to Tricine-SDS-PAGE and Western blot analysis. (B) Tricine-SDS-PAGE profiles of fractions from gel filtration chromatography. The numbers correspond to elution profiles shown in panel A. Ten microliters were applied to Tricine-SDS-PAGE from 1 ml of the fraction. “M” indicates the protein marker. (C) Western blot analysis using anti-His antibody and the same samples in Tricine-SDS-PAGE shown in panel B.

    Article Snippet: Gel-filtration chromatography Protein solutions after purification using HiTrap Chelating HP column were applied to a HiLoad 16/60 Superdex 200 prep-grade column (GE Healthcare).

    Techniques: Filtration, Chromatography, Purification, SDS Page, Western Blot, Marker