gel extraction kit  (Qiagen)

 
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    Name:
    MinElute Gel Extraction Kit
    Description:
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    Catalog Number:
    28604
    Price:
    136
    Category:
    MinElute Gel Extraction Kit
    Buy from Supplier


    Structured Review

    Qiagen gel extraction kit
    MinElute Gel Extraction Kit
    For gel extraction of up to 5 μg DNA fragments 70 bp to 4 kb in low elution volumes Kit contents Qiagen MinElute Gel Extraction Kit 50 MinElute Spin Columns 5g Binding Capacity 10L Elution Volume Tube Format Gel Extraction Technology 70 bp to 4 kb Fragment Size Manual Processing DNA Sample Fast Procedure and Easy Handling High Reproducible Recoveries For Gel Extraction of up to 5μg DNA fragments in Low Elution Volumes Includes 50 MinElute Spin Columns Buffers Collection Tubes 2mL Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/gel extraction kit/product/Qiagen
    Average 99 stars, based on 1225 article reviews
    Price from $9.99 to $1999.99
    gel extraction kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Phosphatidylinositol 3-Kinase (PI3K) δ blockade increases genomic instability in B cells"

    Article Title: Phosphatidylinositol 3-Kinase (PI3K) δ blockade increases genomic instability in B cells

    Journal: Nature

    doi: 10.1038/nature21406

    Ibrutinib increases AID expression and the frequency of translocations to AID on- and off-target sites in mouse activated B cells a , AKT phosphorylation was detected by Western Blot in mouse activated B cells treated with DMSO, idelalisib, duvelisib or ibrutinib (1 μM) for the indicated time points (n = 2 biological replicates). For gel source data, see Supplementary Figure 1 . b , MEC1 and Mino human lymphoma cells were treated with the indicated inhibitors (1 μM) and AKT phosphorylation was evaluated by Western Blot (n = 3 biological replicates). c, Viable cells were counted at the indicated time points by Trypan Blue exclusion in activated B cells treated with DMSO or ibrutinib (1 μM). Data are expressed as mean ± s.d. (n=3). P values calculated by two-tailed Student’s t -test. d, Western blot for AID protein expression in activated B cells treated with 1 μM ibrutinib. The DMSO panel from Fig. 1a is shown for comparison (n=3 biological replicates). e, Aicda mRNA levels analyzed by qRT-PCR in activated B cells treated with DMSO or ibrutinib (1 μM). Data are expressed as mean ± s.d. (n = 3 technical replicates, n = 3 biological replicates). * P
    Figure Legend Snippet: Ibrutinib increases AID expression and the frequency of translocations to AID on- and off-target sites in mouse activated B cells a , AKT phosphorylation was detected by Western Blot in mouse activated B cells treated with DMSO, idelalisib, duvelisib or ibrutinib (1 μM) for the indicated time points (n = 2 biological replicates). For gel source data, see Supplementary Figure 1 . b , MEC1 and Mino human lymphoma cells were treated with the indicated inhibitors (1 μM) and AKT phosphorylation was evaluated by Western Blot (n = 3 biological replicates). c, Viable cells were counted at the indicated time points by Trypan Blue exclusion in activated B cells treated with DMSO or ibrutinib (1 μM). Data are expressed as mean ± s.d. (n=3). P values calculated by two-tailed Student’s t -test. d, Western blot for AID protein expression in activated B cells treated with 1 μM ibrutinib. The DMSO panel from Fig. 1a is shown for comparison (n=3 biological replicates). e, Aicda mRNA levels analyzed by qRT-PCR in activated B cells treated with DMSO or ibrutinib (1 μM). Data are expressed as mean ± s.d. (n = 3 technical replicates, n = 3 biological replicates). * P

    Techniques Used: Expressing, Western Blot, Two Tailed Test, Quantitative RT-PCR

    PI3Kδ inhibitors and ibrutinib increase c-myc DSB formation and the incidence of plasma cell (PC) tumor in mice a, Detailed view of the distribution of rearrangements (deletions or inversions) in the c-myc locus in mice treated as above. Numbers of translocation junctions in focal clusters are indicated in bold. b , RPM Frequency of rearrangements (deletions or inversions) in the c-myc locus in GC B cell from mice treated in vivo with the idelalisib or duvelisib. Junctions within ±300 bp of primer region were excluded. Significance is calculated as false discovery rate ( FDR ) by comparing idelalisib or duvelisib to DMSO treated mouse as indicated in the Methods. ** FDR ≤ 0.01. c , Schematic representation of the experimental outline of pristane-induced PCT in mice treated with PI3Kδ inhibitors and ibrutinib. The mice were treated in two independent biological experimental replicates, each consisting of 6 mice per group. d, Direct PCR assay for Igh/c-myc translocation in mice with PC tumors. Translocations from c-myc to the IgHα locus are shown. Translocations for the only mouse in the vehicle group and from three example mice from treated groups are shown. Bands were purified from gels and were sequenced to confirm the Igh/c-myc translocation junction. For gel source data, see Supplementary Figure 1 . e, Development of PC tumor in mice induced with pristane and treated with idelalisib, duvelisib or ibrutinib is plotted over time. The presence of PC tumors was confirmed by histology (n = 12 for each treatment in 2 independent cohorts of 6 mice). P values calculated by Log-rank (Mantel-Cox) test. f , Example histology of PC tumors in mice induced with pristane and treated with the indicated drugs. Magnification 40x; Scale bar = 50μm; Insets: high magnification image of clusters of atypical plasma cells.
    Figure Legend Snippet: PI3Kδ inhibitors and ibrutinib increase c-myc DSB formation and the incidence of plasma cell (PC) tumor in mice a, Detailed view of the distribution of rearrangements (deletions or inversions) in the c-myc locus in mice treated as above. Numbers of translocation junctions in focal clusters are indicated in bold. b , RPM Frequency of rearrangements (deletions or inversions) in the c-myc locus in GC B cell from mice treated in vivo with the idelalisib or duvelisib. Junctions within ±300 bp of primer region were excluded. Significance is calculated as false discovery rate ( FDR ) by comparing idelalisib or duvelisib to DMSO treated mouse as indicated in the Methods. ** FDR ≤ 0.01. c , Schematic representation of the experimental outline of pristane-induced PCT in mice treated with PI3Kδ inhibitors and ibrutinib. The mice were treated in two independent biological experimental replicates, each consisting of 6 mice per group. d, Direct PCR assay for Igh/c-myc translocation in mice with PC tumors. Translocations from c-myc to the IgHα locus are shown. Translocations for the only mouse in the vehicle group and from three example mice from treated groups are shown. Bands were purified from gels and were sequenced to confirm the Igh/c-myc translocation junction. For gel source data, see Supplementary Figure 1 . e, Development of PC tumor in mice induced with pristane and treated with idelalisib, duvelisib or ibrutinib is plotted over time. The presence of PC tumors was confirmed by histology (n = 12 for each treatment in 2 independent cohorts of 6 mice). P values calculated by Log-rank (Mantel-Cox) test. f , Example histology of PC tumors in mice induced with pristane and treated with the indicated drugs. Magnification 40x; Scale bar = 50μm; Insets: high magnification image of clusters of atypical plasma cells.

    Techniques Used: Mouse Assay, Translocation Assay, In Vivo, Polymerase Chain Reaction, Purification

    Phosphatidylinositol 3-Kinase (PI3K)δ blockade increases AID expression and CSR in activated mouse B cells a , Western blot for AID protein from B cells treated with the indicated inhibitors (1 μM) (n = 3 biological replicates). For gel source data, see Supplementary Figure 1 . b , Aicda mRNA levels were analyzed by qRT-PCR. Data are expressed as mean ± s.d. (n = 3 biological replicates).. ** P ≤ 0.01, *** P ≤ 0.001, two-tailed Student’s t -test from idelalisib or duvelisib vs DMSO-treated B cells. c, GRO-Seq profiles of Aicda gene in B cells at 48 h after activation (n = 2 biological replicates). Blue profiles: sense transcription, Red profiles: antisense transcription. d, Quantification of GRO-Seq sense and antisense reads per kilobase per million mapped reads (RPKM) in the Aicda gene, ** P ≤ 0.01 ,***P ≤ 0.001, multiple test adjusted. e, IgG 1 CSR in activated B cells. Data are expressed as mean ± s.d. (n = 3 biological replicates). ** P ≤ 0.01, *** P ≤ 0.001, two-tailed Student’s t -test from idelalisib or duvelisib vs DMSO treated B cells
    Figure Legend Snippet: Phosphatidylinositol 3-Kinase (PI3K)δ blockade increases AID expression and CSR in activated mouse B cells a , Western blot for AID protein from B cells treated with the indicated inhibitors (1 μM) (n = 3 biological replicates). For gel source data, see Supplementary Figure 1 . b , Aicda mRNA levels were analyzed by qRT-PCR. Data are expressed as mean ± s.d. (n = 3 biological replicates).. ** P ≤ 0.01, *** P ≤ 0.001, two-tailed Student’s t -test from idelalisib or duvelisib vs DMSO-treated B cells. c, GRO-Seq profiles of Aicda gene in B cells at 48 h after activation (n = 2 biological replicates). Blue profiles: sense transcription, Red profiles: antisense transcription. d, Quantification of GRO-Seq sense and antisense reads per kilobase per million mapped reads (RPKM) in the Aicda gene, ** P ≤ 0.01 ,***P ≤ 0.001, multiple test adjusted. e, IgG 1 CSR in activated B cells. Data are expressed as mean ± s.d. (n = 3 biological replicates). ** P ≤ 0.01, *** P ≤ 0.001, two-tailed Student’s t -test from idelalisib or duvelisib vs DMSO treated B cells

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Two Tailed Test, Activation Assay

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    Nucleic Acid Electrophoresis:

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Amplification:

    Article Title: Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1
    Article Snippet: .. Amplified fragments were separated by gel electrophoresis, gel-purified (MinElute gel extraction kit, Qiagen), and directly sequenced using the second-round oligonucleotides (Macrogen, South Korea). .. Detection of NaD1 by immunoblot analysis Total soluble proteins were extracted from plant tissue in 50mM H2 SO4 plus 2% (w/v) polyvinylpyrrolidone (PVPP; 1g fresh weight tissue (1.2 ml buffer)–1 ) and insoluble material was removed by centrifugation (18,400 g , 10min).

    Agarose Gel Electrophoresis:

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
    Article Snippet: .. Reaction was carried out with an initial denaturation step at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 65°C for 30 s, 70°C for 1 min, and a final step of 70°C for 10 min. PCR fragments were detected by agarose gel electrophoresis, and extracted using DNA Gel Extraction Kit (Qiagen, Hilden, Germany). .. By T4 DNA ligase (New England Biolabs, Ipswich, MA), the extracted fragment digested with NcoI and BamHI was ligased to vector phoA at 4°C, and was transformed into DH5α competent cells.

    Purification:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: Catalysts from synthetic genetic polymers
    Article Snippet: .. Bands of appropriate size were purified using a gel extraction kit (Qiagen, Netherlands) as per manufacturer’s instructions. .. Purified DNA was used as the polyclonal template for either sequencing library PCR (see below) or large scale preparative PCR (2ml) for generation of DNA templates for XNA synthesis.

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

    Polymerase Chain Reaction:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
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    Article Title: Genetic transformation of the dinoflagellate chloroplast
    Article Snippet: .. PCR products were purified from excised gel pieces using the MinElute gel extraction kit (Qiagen). .. Some PCR products were directly sequenced after gel extraction whilst others were ligated into the pGEM-T Easy plasmid vector (Promega), following the manufacturer’s instructions.

    Article Title: HLA genotyping by next-generation sequencing of complementary DNA
    Article Snippet: .. The following cycling conditions were used: 1 cycle of 94 °C for 30 s, 40 cycles of 94 °C for 30 s, 65 °C for 30 s, and 68 °C for 1 min. After mixing each PCR product, gel extraction (range from 200 bp to 500 bp) was performed using the MinElute Gel Extraction Kit (Qiagen, Hilden, Germany). .. The concentration of the purified products was measured using the Quanti-iT™ dsDNA Assay Kit, Broad range.

    Gel Extraction:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
    Article Snippet: .. PCR amplicons corresponding to the expected length of predicted translocations and only present in cells treated with sgRNA were purified (QIAGEN Gel Extraction kit) and analyzed by Sanger sequencing. .. mRNA analysis Immortalized myoblasts were differentiated into myofibers by replacing the growth medium with DMEM supplemented with 1% insulin-transferrin-selenium (Invitrogen #51500056) and 1% penicillin/streptomycin (Invitrogen #15140) for 5 days before the cells were trypsinized and collected.

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    Article Title: Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA
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    Article Title: Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq
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    Sequencing:

    Article Title: Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
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    Staining:

    Article Title: Biogeography of Soil Bacterial Networks along a Gradient of Cropping Intensity
    Article Snippet: .. Crude DNA was then purified using a MinElute gel extraction kit (Qiagen, France) and quantified using a QuantiFluor staining kit (Promega, USA), prior to further investigations. .. PCR amplification and pyrosequencing of 16S rRNA gene sequences A 16S rRNA gene fragment targeting the V3-V4 regions to characterize bacterial diversity was amplified using the primers F479 (5′-CAGCMGCYGCNGTAANAC-3′) and R888 (5′-CCGYCAATTCMTTTRAGT-3′) with the method described previously .

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  • 99
    Qiagen no 114391 5g qiaquick gel extractio kit
    No 114391 5g Qiaquick Gel Extractio Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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