gel extraction kit  (Millipore)


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  • 95
    Name:
    Gelatin
    Description:

    Catalog Number:
    48723
    Price:
    None
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    Structured Review

    Millipore gel extraction kit

    https://www.bioz.com/result/gel extraction kit/product/Millipore
    Average 95 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    gel extraction kit - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    Invasion Assay:

    Article Title: Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice
    Article Snippet: .. In vitro ECM invasion assay Cell invasion in vitro was demonstrated using 24-well Transwell units (Corning, NY, USA) with 8-μm pore size polycarbonate filter coated with ECMatrix gel (Chemicon, Temecula, CA, USA) to form a continuous thin layer. ..

    In Vitro:

    Article Title: Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice
    Article Snippet: .. In vitro ECM invasion assay Cell invasion in vitro was demonstrated using 24-well Transwell units (Corning, NY, USA) with 8-μm pore size polycarbonate filter coated with ECMatrix gel (Chemicon, Temecula, CA, USA) to form a continuous thin layer. ..

    Centrifugation:

    Article Title: Fluid Gels: a New Feedstock for High Viscosity Jetting
    Article Snippet: .. On centrifugation of Sigma and Luxara fluid gel samples (described above in the section) the volume fraction of particles versus continuous phase was found to be much less for Luxara gel particles than Sigma ones, therefore in the Luxara sample particles would not exhibit a ‘jamming’ phenomenon on formation as seen in the Sigma sample. ..

    Cell Culture:

    Article Title: CRP2, a new invadopodia actin bundling factor critically promotes breast cancer cell invasion and metastasis
    Article Snippet: .. To investigate CRP2 localization in invadopodia, cells were cultured on Cy3-conjugated gelatin-coated coverslips (QCMTM Gelatin Invadopodia Assay, Millipore) for 6 h and CRP2-GFP was co-localized with cortactin (antibody clone 4F11, Millipore) and actin (Acti-stainTM 670 phalloidin, Cytoskeleton). .. To localize CRP2 in elongated invadopodia, cells were grown on EHS matrix-coated ThinCertTM Tissue Culture Inserts (Greiner) with 1-μm-diameter pores [ ].

    Article Title: Atherogenesis May Involve the Prooxidant and Proinflammatory Effects of Ferryl Hemoglobin
    Article Snippet: .. EC Monolayer Permeability Assay HUVECs were cultured on gelatin-coated hanging cell culture inserts (Millipore Corporation, Billerica, MA, USA). ..

    Invadopodia Assay:

    Article Title: CRP2, a new invadopodia actin bundling factor critically promotes breast cancer cell invasion and metastasis
    Article Snippet: .. To investigate CRP2 localization in invadopodia, cells were cultured on Cy3-conjugated gelatin-coated coverslips (QCMTM Gelatin Invadopodia Assay, Millipore) for 6 h and CRP2-GFP was co-localized with cortactin (antibody clone 4F11, Millipore) and actin (Acti-stainTM 670 phalloidin, Cytoskeleton). .. To localize CRP2 in elongated invadopodia, cells were grown on EHS matrix-coated ThinCertTM Tissue Culture Inserts (Greiner) with 1-μm-diameter pores [ ].

    Permeability:

    Article Title: Atherogenesis May Involve the Prooxidant and Proinflammatory Effects of Ferryl Hemoglobin
    Article Snippet: .. EC Monolayer Permeability Assay HUVECs were cultured on gelatin-coated hanging cell culture inserts (Millipore Corporation, Billerica, MA, USA). ..

    Fluorescence In Situ Hybridization:

    Article Title: Evidence for a novel human-specific xeno-auto-antibody response against vascular endothelium
    Article Snippet: .. Membranes were blocked overnight at 4°C with 0.5% Neu5Gc-free cold water fish gelatin (Sigma-Aldrich) in TBST. .. Primary antibodies were incubated for 5 hours at 4°C with either biotinylated anti-Neu5Gc antibodies purified from individual human sera, diluted 1:100 in TBST, or chicken anti-Neu5Gc antibody (cGcAb), diluted 1:50 000 in TBST.

    Derivative Assay:

    Article Title: Cleavage of Nidogen-1 by Cathepsin S Impairs Its Binding to Basement Membrane Partners
    Article Snippet: .. Laminin-211/221 (abbreviated forms corresponding respectively to chains: α2β1γ1/α2β2γ1) and type IV collagen (both from human placenta), perlecan and basement membrane extract, ECM gel (both derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma) were obtained from Sigma-Aldrich. .. Fibronectin (from human plasma) was from Calbiochem.

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  • 99
    Millipore montage dna gel extraction kit
    Microscopic analysis of the <t>Helicobacter</t> pullorum positive Meckel´s diverticulum case. Low power view of a histological section from Meckel´s diverticulum, positive for Helicobacter pullorum ( H. pullorum ) <t>DNA,</t> in the heterotopic area. There is an unusually well developed lymphatic tissue with germinal centres and a predominance of lymphocytes in the non-heterotopic area. Plasma cells were mainly found in the heterotopic area. The small arrows in the big square, point to a small strip of gastric heterotopia. This area displays at higher magnification in the inset. Large arrows point to gastric glands of corpus type. Blue without arrows in the photo indicates intestinal mucus. Alcian blue-PAS staining pH 2.5.
    Montage Dna Gel Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/montage dna gel extraction kit/product/Millipore
    Average 99 stars, based on 147 article reviews
    Price from $9.99 to $1999.99
    montage dna gel extraction kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Millipore agarose gel dna extraction kit
    gins2 u773/u773 mutants show cell proliferation defects. A, B) The intensity of fluorescence of BrdU labeling in the OT of a 3 <t>dpf</t> wild type embryo shows a medio-lateral gradient. In contrast, mutant embryos showed a uniform distribution of the BrdU label. Dorsal views of the OT are shown, with anterior to the left. Midline is marked by the dashed line, yellow bars denote the proliferative area with a medial to lateral gradient of fluorescence in wildtype. C, D) Representative histograms of flow cytometry analysis of wildtype and mutant samples. E) <t>DNA</t> content analysis of propidium-iodide-labeled cells isolated from the head region showed no obvious change in the number of cells with 2C (p=0.15, n=6), and a slight, but significant decrease in the number of cells with 4C (p=0.01, n=6 independent samples). F, G) Regenerated area of the caudal fin in wildtype siblings and mutant embryos following amputation at 2 dpf. H) At 3 days post injury (dpi) the size of the regenerated fin fold was slightly but significantly smaller in mutant larvae than in controls (p=0.038, n=19).
    Agarose Gel Dna Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agarose gel dna extraction kit/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agarose gel dna extraction kit - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Microscopic analysis of the Helicobacter pullorum positive Meckel´s diverticulum case. Low power view of a histological section from Meckel´s diverticulum, positive for Helicobacter pullorum ( H. pullorum ) DNA, in the heterotopic area. There is an unusually well developed lymphatic tissue with germinal centres and a predominance of lymphocytes in the non-heterotopic area. Plasma cells were mainly found in the heterotopic area. The small arrows in the big square, point to a small strip of gastric heterotopia. This area displays at higher magnification in the inset. Large arrows point to gastric glands of corpus type. Blue without arrows in the photo indicates intestinal mucus. Alcian blue-PAS staining pH 2.5.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix

    doi: 10.3748/wjg.v17.i36.4104

    Figure Lengend Snippet: Microscopic analysis of the Helicobacter pullorum positive Meckel´s diverticulum case. Low power view of a histological section from Meckel´s diverticulum, positive for Helicobacter pullorum ( H. pullorum ) DNA, in the heterotopic area. There is an unusually well developed lymphatic tissue with germinal centres and a predominance of lymphocytes in the non-heterotopic area. Plasma cells were mainly found in the heterotopic area. The small arrows in the big square, point to a small strip of gastric heterotopia. This area displays at higher magnification in the inset. Large arrows point to gastric glands of corpus type. Blue without arrows in the photo indicates intestinal mucus. Alcian blue-PAS staining pH 2.5.

    Article Snippet: Helicobacter specific PCR products were purified from agarose gels using the Montage DNA Gel Extraction Kit (Millipore, Bedford, MA, United States), according to the manufacturer’s instructions.

    Techniques: Stripping Membranes, Staining

    Schematic of small DNA fragment (SDF) generation and PCR analysis of SFHR A. SDF (783bp) containing the ΔF508 mutation and a KpnI restriction enzyme cleavage site was synthesized using primers mCF1 and mCF15, localized within introns 9 and 10

    Journal:

    Article Title: CFTR gene targeting in mouse embryonic stem cells mediated by Small Fragment Homologous Replacement (SFHR)

    doi:

    Figure Lengend Snippet: Schematic of small DNA fragment (SDF) generation and PCR analysis of SFHR A. SDF (783bp) containing the ΔF508 mutation and a KpnI restriction enzyme cleavage site was synthesized using primers mCF1 and mCF15, localized within introns 9 and 10

    Article Snippet: Before transfection the SDF was used, always gel and ethanol purified (DNA gel extraction kit; Millipore, Bedford, MA).

    Techniques: Polymerase Chain Reaction, Mutagenesis, Synthesized

    gins2 u773/u773 mutants show cell proliferation defects. A, B) The intensity of fluorescence of BrdU labeling in the OT of a 3 dpf wild type embryo shows a medio-lateral gradient. In contrast, mutant embryos showed a uniform distribution of the BrdU label. Dorsal views of the OT are shown, with anterior to the left. Midline is marked by the dashed line, yellow bars denote the proliferative area with a medial to lateral gradient of fluorescence in wildtype. C, D) Representative histograms of flow cytometry analysis of wildtype and mutant samples. E) DNA content analysis of propidium-iodide-labeled cells isolated from the head region showed no obvious change in the number of cells with 2C (p=0.15, n=6), and a slight, but significant decrease in the number of cells with 4C (p=0.01, n=6 independent samples). F, G) Regenerated area of the caudal fin in wildtype siblings and mutant embryos following amputation at 2 dpf. H) At 3 days post injury (dpi) the size of the regenerated fin fold was slightly but significantly smaller in mutant larvae than in controls (p=0.038, n=19).

    Journal: bioRxiv

    Article Title: The GINS complex is required for the survival of rapidly proliferating retinal and tectal progenitor cells during zebrafish development

    doi: 10.1101/2020.02.09.940767

    Figure Lengend Snippet: gins2 u773/u773 mutants show cell proliferation defects. A, B) The intensity of fluorescence of BrdU labeling in the OT of a 3 dpf wild type embryo shows a medio-lateral gradient. In contrast, mutant embryos showed a uniform distribution of the BrdU label. Dorsal views of the OT are shown, with anterior to the left. Midline is marked by the dashed line, yellow bars denote the proliferative area with a medial to lateral gradient of fluorescence in wildtype. C, D) Representative histograms of flow cytometry analysis of wildtype and mutant samples. E) DNA content analysis of propidium-iodide-labeled cells isolated from the head region showed no obvious change in the number of cells with 2C (p=0.15, n=6), and a slight, but significant decrease in the number of cells with 4C (p=0.01, n=6 independent samples). F, G) Regenerated area of the caudal fin in wildtype siblings and mutant embryos following amputation at 2 dpf. H) At 3 days post injury (dpi) the size of the regenerated fin fold was slightly but significantly smaller in mutant larvae than in controls (p=0.038, n=19).

    Article Snippet: At 2 dpf embryos were anesthetized, and embedded into 0.8% low-melting point agarose in E3.

    Techniques: Fluorescence, Labeling, Mutagenesis, Flow Cytometry, Isolation