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Promega gel clean up kit
Gel Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel clean up kit/product/Promega
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
gel clean up kit - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Clone Assay:

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: Paragraph title: Phage PCR amplification, cloning and sequencing ... To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Amplification:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: Paragraph title: Viral DNA extraction, amplification and sequencing ... Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega).

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: Paragraph title: Phage PCR amplification, cloning and sequencing ... To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Article Title: Variant in SCYL1 gene causes aberrant splicing in a family with cerebellar ataxia, recurrent episodes of liver failure, and growth retardation
Article Snippet: Complementary DNA was synthesized, and amplification of SCYL1 was performed by polymerase chain reaction (PCR) using custom-planned primers: forward: 5′-CGTTGGGAATATACCTCAAGGCG-3′, reverse 5′-TGAAGACT TCCCAAATGAGGCAGC-3′. .. Bands were extracted using a gel clean-up kit (Promega) and sent for confirmation via Sanger sequencing.

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: Therefore, for a proper comparison all samples were amplified by Multiple Displacement Amplification (MDA) to increase the concentration of viral DNA suitable for metagenomic analysis . .. After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform.

Synthesized:

Article Title: Variant in SCYL1 gene causes aberrant splicing in a family with cerebellar ataxia, recurrent episodes of liver failure, and growth retardation
Article Snippet: Complementary DNA was synthesized, and amplification of SCYL1 was performed by polymerase chain reaction (PCR) using custom-planned primers: forward: 5′-CGTTGGGAATATACCTCAAGGCG-3′, reverse 5′-TGAAGACT TCCCAAATGAGGCAGC-3′. .. Bands were extracted using a gel clean-up kit (Promega) and sent for confirmation via Sanger sequencing.

TA Cloning:

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. 4 µl of each purified PCR product was cloned into a pCR4-TOPO vector using the TOPO TA Cloning kit (Invitrogen).

Electrophoresis:

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: The results of the PCR amplifications were visualized by electrophoresis on a 1% agarose gel stained with ethidium bromide on a UV transilluminator. .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea).

Incubation:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: Briefly, each filter was incubated at 56 °C for one hour with 20 mM EDTA, 10% SDS and 50 μg ml−1 proteinase K. Viral DNA was purified through two subsequent phenol-chloroform treatment steps followed by isopropanol precipitation. .. Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega).

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. Transformed E. coli were plated on LB + carbenicillin plates and incubated overnight at 37 °C.

Cell Culture:

Article Title: Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes
Article Snippet: 2.4 Calibration curve Calibration standards were prepared using DNA extracted from cultured Human Epithelial type-2 (HEp-2) cells, which had been infected with Ct strain A2497. .. PCR products were purified and extracted using the Promega gel clean-up kit and Wizard SV PCR spin columns (Promega, Madison, USA) according to manufacturer's guidelines.

Transformation Assay:

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. OneShot TOP10 E. coli cells (Life Technologies) were transformed with the recombinant plasmids through heat shock according to the manufacturer’s protocol.

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Infection:

Article Title: Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes
Article Snippet: 2.4 Calibration curve Calibration standards were prepared using DNA extracted from cultured Human Epithelial type-2 (HEp-2) cells, which had been infected with Ct strain A2497. .. PCR products were purified and extracted using the Promega gel clean-up kit and Wizard SV PCR spin columns (Promega, Madison, USA) according to manufacturer's guidelines.

Generated:

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. The resulting chromatograms were analysed within Geneious v. 6.1.8 and aligned with the consensus sequences generated by the whole‐genome sequencing analysis using the C lustal W plugin within Geneious v. 6.1.8.

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform. .. To assess the potential biases induced by MDA, we compared MDA treated and un-treated viromes generated from sediment samples collected in the same deep-sea site of the Mediterranean Sea.

Polymerase Chain Reaction:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: .. Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega). .. Before pyrosequencing, potential contamination due to prokaryotic and eukaryotic DNA was checked in the viral DNA samples by PCR targeting 16S and 18S rRNA genes and gel electrophoresis analysis.

Article Title: Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes
Article Snippet: .. PCR products were purified and extracted using the Promega gel clean-up kit and Wizard SV PCR spin columns (Promega, Madison, USA) according to manufacturer's guidelines. .. PCR products were then diluted 1:107 in 1 mM Tris-Cl 0.1 mM EDTA (0.1 × TE) buffer on a background of 2 ng/μL herring sperm DNA (Sigma Aldrich, St Louis, USA).

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: .. To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. 4 µl of each purified PCR product was cloned into a pCR4-TOPO vector using the TOPO TA Cloning kit (Invitrogen).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: .. After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform. .. To assess the potential biases induced by MDA, we compared MDA treated and un-treated viromes generated from sediment samples collected in the same deep-sea site of the Mediterranean Sea.

Sonication:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: Each filter was added with virus-free milliQ water and sonicated (three times for 1 minute, with 30 second of manual shaking between each cycle) to detach viral particles. .. Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega).

Recombinant:

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. OneShot TOP10 E. coli cells (Life Technologies) were transformed with the recombinant plasmids through heat shock according to the manufacturer’s protocol.

DNA Extraction:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: Paragraph title: Viral DNA extraction, amplification and sequencing ... Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega).

Nucleic Acid Electrophoresis:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega). .. Before pyrosequencing, potential contamination due to prokaryotic and eukaryotic DNA was checked in the viral DNA samples by PCR targeting 16S and 18S rRNA genes and gel electrophoresis analysis.

Multiple Displacement Amplification:

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: .. After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform. .. To assess the potential biases induced by MDA, we compared MDA treated and un-treated viromes generated from sediment samples collected in the same deep-sea site of the Mediterranean Sea.

Isolation:

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: Viral assemblage composition of MDA treated and un-treated samples Independent from the procedure used for viral recovery, the amounts of viral DNA isolated from the different benthic deep-sea sites with the exception of the Black Sea were not sufficient to be directly sequenced. .. After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform.

Purification:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: .. Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega). .. Before pyrosequencing, potential contamination due to prokaryotic and eukaryotic DNA was checked in the viral DNA samples by PCR targeting 16S and 18S rRNA genes and gel electrophoresis analysis.

Article Title: Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes
Article Snippet: .. PCR products were purified and extracted using the Promega gel clean-up kit and Wizard SV PCR spin columns (Promega, Madison, USA) according to manufacturer's guidelines. .. PCR products were then diluted 1:107 in 1 mM Tris-Cl 0.1 mM EDTA (0.1 × TE) buffer on a background of 2 ng/μL herring sperm DNA (Sigma Aldrich, St Louis, USA).

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: .. To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. 4 µl of each purified PCR product was cloned into a pCR4-TOPO vector using the TOPO TA Cloning kit (Invitrogen).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: .. After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform. .. To assess the potential biases induced by MDA, we compared MDA treated and un-treated viromes generated from sediment samples collected in the same deep-sea site of the Mediterranean Sea.

Sequencing:

Article Title: Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses
Article Snippet: Paragraph title: Viral DNA extraction, amplification and sequencing ... Pooled replicates were purified using Wizard PCR and Gel Clean-up kit (Promega).

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: .. To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. 4 µl of each purified PCR product was cloned into a pCR4-TOPO vector using the TOPO TA Cloning kit (Invitrogen).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Article Title: Variant in SCYL1 gene causes aberrant splicing in a family with cerebellar ataxia, recurrent episodes of liver failure, and growth retardation
Article Snippet: .. Bands were extracted using a gel clean-up kit (Promega) and sent for confirmation via Sanger sequencing. .. Sequence alignment was performed using vector NTI software.

Plasmid Preparation:

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. 4 µl of each purified PCR product was cloned into a pCR4-TOPO vector using the TOPO TA Cloning kit (Invitrogen).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea). .. In the instances in which multiple amplicons were identified from initial sequencing for SIX1 and could not be selectively amplified using PCR, the amplicons were cloned using the pCR2.1 vector with the TOPO TA kit (Invitrogen Life Technologies, Carlsbad, CA, USA) and transformed into Escherichia coli Top10 competent cells (Invitrogen), according to the manufacturer's instructions.

Article Title: Variant in SCYL1 gene causes aberrant splicing in a family with cerebellar ataxia, recurrent episodes of liver failure, and growth retardation
Article Snippet: Bands were extracted using a gel clean-up kit (Promega) and sent for confirmation via Sanger sequencing. .. Sequence alignment was performed using vector NTI software.

Software:

Article Title: Variant in SCYL1 gene causes aberrant splicing in a family with cerebellar ataxia, recurrent episodes of liver failure, and growth retardation
Article Snippet: Bands were extracted using a gel clean-up kit (Promega) and sent for confirmation via Sanger sequencing. .. Sequence alignment was performed using vector NTI software.

Agarose Gel Electrophoresis:

Article Title: Wolbachia co-infection in a hybrid zone: discovery of horizontal gene transfers from two Wolbachia supergroups into an animal genome
Article Snippet: .. To clone and sequence the orf7 gene, PCR products were run on a 1% TBE agarose gel, then excised and purified using the Wizard PCR and Gel Clean-up Kit (Promega). .. 4 µl of each purified PCR product was cloned into a pCR4-TOPO vector using the TOPO TA Cloning kit (Invitrogen).

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: The results of the PCR amplifications were visualized by electrophoresis on a 1% agarose gel stained with ethidium bromide on a UV transilluminator. .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea).

Next-Generation Sequencing:

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: A larger collection of 89 Foc isolates representing all VCGs and including the isolates in the NGS panel were screened for the Foc‐SIX genes using PCR (Table S , see Supporting Information). .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea).

Concentration Assay:

Article Title: From virus isolation to metagenome generation for investigating viral diversity in deep-sea sediments
Article Snippet: Therefore, for a proper comparison all samples were amplified by Multiple Displacement Amplification (MDA) to increase the concentration of viral DNA suitable for metagenomic analysis . .. After MDA, viral DNA was purified (using Wizard PCR and Gel Clean-up kit, Promega) and then sequenced onto a 454 Titanium FLX platform.

Staining:

Article Title: Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer
Article Snippet: The results of the PCR amplifications were visualized by electrophoresis on a 1% agarose gel stained with ethidium bromide on a UV transilluminator. .. Amplicons were purified using the Wizard SV PCR and Gel Clean‐Up kit (Promega), and sequenced in the forward and reverse directions using the PCR amplification primers in a single‐pass Sanger sequencing reaction by Macrogen (Seoul, South Korea).

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    Promega wizard sv gel and pcr clean up system
    Identification of osKaR insertion site Arbitrary-primed <t>PCR</t> (AP-PCR) is a quick method to precisely identify the genomic region where a transposon has inserted. The following is specific for osKaR insertions: genomic DNA of the osKaR mutant is extracted (see Basic Protocol 1) and used for a semi-random PCR using the osKaR -specific primer oPCR1 and the random primer Deg3. The Deg3 primer consists of an 11-nucleotide random primer (in blue) with a 25-nucleotide specific tail (in red). The resulting PCR product is purified and used for a 2 nd PCR using the osKaR -specific primer Anchor1 and the Deg3-tail specific primer <t>Deg4.</t> The resulting PCR product is purified and DNA sequencing is performed using the osKaR -specific primer Anchor2.
    Wizard Sv Gel And Pcr Clean Up System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard sv gel and pcr clean up system/product/Promega
    Average 99 stars, based on 326 article reviews
    Price from $9.99 to $1999.99
    wizard sv gel and pcr clean up system - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Promega wizard dna cleanup kit
    Parallel changes in PFGE profile and <t>lytA</t> hybridization pattern in selected strains of S. pneumoniae . Lanes 1 and 15 were loaded with low-range PFGE markers. Lanes 2, 9, and 14 contain Sma ). Strains SW 96 and SW 95 are from Sweden. (A) Sma I digest of total <t>DNA</t> separated by PFGE. (B) Hybridization of a Southern blot of the gel in panel A with the lytA gene probe. Numbers in the center indicate molecular sizes in kilobases.
    Wizard Dna Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard dna cleanup kit/product/Promega
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    wizard dna cleanup kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Promega pcr clean up kit
    Plasmid toolbox for the construction of <t>CRISPR/Cas9-HCAdV</t> genomes. ( A ) Schematic presentation of intermediate CRISPR/Cas9 shuttle plasmids for simple gRNA manipulation and multiplexing and subsequent transfer of the customized CRISPR/Cas9 machinery into the HCAdV genome. Option 1: pShV-CBh-Cas9-gRNA for constitutive Cas9 expression. Option 2: pShV-TRE-Cas9-TeOn3G-gRNA for inducible Cas9 expression utilizing the TetOn3G system. Black arrowheads indicate unique restriction enzyme sites for insertion of further gRNA expression units. ( B ) Workflow for gRNA customization and multiplexing of the CRISPR/Cas9 machinery. Step1: Complementary annealed gRNA oligonucleotides are separately inserted between the Bsa I restriction enzyme sites resulting in pShV-CBh-Cas9-gRNA1, pShV-CBh-Cas9-gRNA2 and pShV-CBh-Cas9- gRNA3. Step 2: Customized gRNA expression units gRNA1 and gRNA2 are amplified by <t>PCR</t> using primers generating desired restriction enzyme sites. Step 3: gRNA1 and 2 are inserted into the respective restriction enzyme site within pShV-CBh-Cas9-gRNA1 resulting in pShV-CBh-Cas9-CBh-gRNA1-gRNA2-gRNA3. ( C ) Transfer of customized CRISPR/Cas9 transgenes into the HCAdV genomes. Option 1: Released CRISPR/Cas9 transgene cassettes flanked by homology arms are inserted into pHCAdV-HOM-CcdB-AMP-HOM replacing the CcdB-Amp R cassette. Option 2: Endonuclease guided cloning into pAd-FTC utilizing PI- Sce I and I- Ceu I. HOM, homology arms for homologous recombination into pHCAdV-HOM-CCBD-AMP-HOM; CBh-P, constitutive hybrid CMV enhancer/chicken β-actin promotor; TRE-P, inducible tetracycline responsible element promotor; TetOn3G, TetOn3G transactivator; Ef1-α-P, Ef1-α-Promotor; Cas9, Streptococcus pyogenes Cas9, gRNA, guide RNA expression unit; U6-P, U6 RNA polymerase III promotor, Kan R , Kanamycin resistance cassette; Amp R ; Ampicillin resistance cassette, Chl R , Chloramphenicol resistance cassette; CcdB, control of cell death B expression cassette; ITR, adenovirus serotype 5 inverted terminal repeat; Ψ, adenovirus serotype 5 packaging signal.
    Pcr Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/Promega
    Average 99 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Identification of osKaR insertion site Arbitrary-primed PCR (AP-PCR) is a quick method to precisely identify the genomic region where a transposon has inserted. The following is specific for osKaR insertions: genomic DNA of the osKaR mutant is extracted (see Basic Protocol 1) and used for a semi-random PCR using the osKaR -specific primer oPCR1 and the random primer Deg3. The Deg3 primer consists of an 11-nucleotide random primer (in blue) with a 25-nucleotide specific tail (in red). The resulting PCR product is purified and used for a 2 nd PCR using the osKaR -specific primer Anchor1 and the Deg3-tail specific primer Deg4. The resulting PCR product is purified and DNA sequencing is performed using the osKaR -specific primer Anchor2.

    Journal: Current protocols in microbiology

    Article Title: Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)

    doi: 10.1002/9780471729259.mc09d03s30

    Figure Lengend Snippet: Identification of osKaR insertion site Arbitrary-primed PCR (AP-PCR) is a quick method to precisely identify the genomic region where a transposon has inserted. The following is specific for osKaR insertions: genomic DNA of the osKaR mutant is extracted (see Basic Protocol 1) and used for a semi-random PCR using the osKaR -specific primer oPCR1 and the random primer Deg3. The Deg3 primer consists of an 11-nucleotide random primer (in blue) with a 25-nucleotide specific tail (in red). The resulting PCR product is purified and used for a 2 nd PCR using the osKaR -specific primer Anchor1 and the Deg3-tail specific primer Deg4. The resulting PCR product is purified and DNA sequencing is performed using the osKaR -specific primer Anchor2.

    Article Snippet: Genomic DNA of GAS osKaR mutant (see Basic Protocol 1) Primers oPCR1, Deg3, Anchor1, Deg4 and Anchor2 (see recipe) Reagents and equipment for PCR Gel and PCR Clean-Up System kit (Wizard SV, Promega Cat. No. A9282)

    Techniques: Polymerase Chain Reaction, Mutagenesis, Purification, DNA Sequencing

    Purified polymerase chain reaction products from the cccj , cdtB , csrA, cst-II, ggt and virB11 genes. Strain: C. jejuni 81176. M, DNA Molecular Weight Marker.

    Journal: Brazilian Journal of Microbiology

    Article Title: Prevalence of virulence genes in strains of Campylobacter jejuni isolated from human, bovine and broiler

    doi:

    Figure Lengend Snippet: Purified polymerase chain reaction products from the cccj , cdtB , csrA, cst-II, ggt and virB11 genes. Strain: C. jejuni 81176. M, DNA Molecular Weight Marker.

    Article Snippet: Sequencing of PCR products Five PCR products of C. jejuni 81176 were purified using the Kit Wizard® SV gel and PCR clean-up system, (Promega, Madison, Wisconsin, U.S) ( ).

    Techniques: Purification, Polymerase Chain Reaction, Molecular Weight, Marker

    Parallel changes in PFGE profile and lytA hybridization pattern in selected strains of S. pneumoniae . Lanes 1 and 15 were loaded with low-range PFGE markers. Lanes 2, 9, and 14 contain Sma ). Strains SW 96 and SW 95 are from Sweden. (A) Sma I digest of total DNA separated by PFGE. (B) Hybridization of a Southern blot of the gel in panel A with the lytA gene probe. Numbers in the center indicate molecular sizes in kilobases.

    Journal: Journal of Clinical Microbiology

    Article Title: Prophage Carriage as a Molecular Epidemiological Marker in Streptococcus pneumoniae

    doi:

    Figure Lengend Snippet: Parallel changes in PFGE profile and lytA hybridization pattern in selected strains of S. pneumoniae . Lanes 1 and 15 were loaded with low-range PFGE markers. Lanes 2, 9, and 14 contain Sma ). Strains SW 96 and SW 95 are from Sweden. (A) Sma I digest of total DNA separated by PFGE. (B) Hybridization of a Southern blot of the gel in panel A with the lytA gene probe. Numbers in the center indicate molecular sizes in kilobases.

    Article Snippet: The 1.2-kb fragment containing the lytA gene was purified from the gel by using the Wizard DNA Cleanup kit.

    Techniques: Hybridization, Southern Blot

    Stability of lytA ). Lanes 1 represent the isolate before extensive serial passage in vitro. Lanes 2 represent the isolate after in vitro passage, as described in Materials and Methods. Numbers in the center indicate molecular sizes in kilobases. (A and C) Sma I digest of total DNA separated by PFGE. (B and D) Hybridization of Southern blots of the gels in panels A and C, respectively, with the lytA gene probe.

    Journal: Journal of Clinical Microbiology

    Article Title: Prophage Carriage as a Molecular Epidemiological Marker in Streptococcus pneumoniae

    doi:

    Figure Lengend Snippet: Stability of lytA ). Lanes 1 represent the isolate before extensive serial passage in vitro. Lanes 2 represent the isolate after in vitro passage, as described in Materials and Methods. Numbers in the center indicate molecular sizes in kilobases. (A and C) Sma I digest of total DNA separated by PFGE. (B and D) Hybridization of Southern blots of the gels in panels A and C, respectively, with the lytA gene probe.

    Article Snippet: The 1.2-kb fragment containing the lytA gene was purified from the gel by using the Wizard DNA Cleanup kit.

    Techniques: In Vitro, Hybridization

    Plasmid toolbox for the construction of CRISPR/Cas9-HCAdV genomes. ( A ) Schematic presentation of intermediate CRISPR/Cas9 shuttle plasmids for simple gRNA manipulation and multiplexing and subsequent transfer of the customized CRISPR/Cas9 machinery into the HCAdV genome. Option 1: pShV-CBh-Cas9-gRNA for constitutive Cas9 expression. Option 2: pShV-TRE-Cas9-TeOn3G-gRNA for inducible Cas9 expression utilizing the TetOn3G system. Black arrowheads indicate unique restriction enzyme sites for insertion of further gRNA expression units. ( B ) Workflow for gRNA customization and multiplexing of the CRISPR/Cas9 machinery. Step1: Complementary annealed gRNA oligonucleotides are separately inserted between the Bsa I restriction enzyme sites resulting in pShV-CBh-Cas9-gRNA1, pShV-CBh-Cas9-gRNA2 and pShV-CBh-Cas9- gRNA3. Step 2: Customized gRNA expression units gRNA1 and gRNA2 are amplified by PCR using primers generating desired restriction enzyme sites. Step 3: gRNA1 and 2 are inserted into the respective restriction enzyme site within pShV-CBh-Cas9-gRNA1 resulting in pShV-CBh-Cas9-CBh-gRNA1-gRNA2-gRNA3. ( C ) Transfer of customized CRISPR/Cas9 transgenes into the HCAdV genomes. Option 1: Released CRISPR/Cas9 transgene cassettes flanked by homology arms are inserted into pHCAdV-HOM-CcdB-AMP-HOM replacing the CcdB-Amp R cassette. Option 2: Endonuclease guided cloning into pAd-FTC utilizing PI- Sce I and I- Ceu I. HOM, homology arms for homologous recombination into pHCAdV-HOM-CCBD-AMP-HOM; CBh-P, constitutive hybrid CMV enhancer/chicken β-actin promotor; TRE-P, inducible tetracycline responsible element promotor; TetOn3G, TetOn3G transactivator; Ef1-α-P, Ef1-α-Promotor; Cas9, Streptococcus pyogenes Cas9, gRNA, guide RNA expression unit; U6-P, U6 RNA polymerase III promotor, Kan R , Kanamycin resistance cassette; Amp R ; Ampicillin resistance cassette, Chl R , Chloramphenicol resistance cassette; CcdB, control of cell death B expression cassette; ITR, adenovirus serotype 5 inverted terminal repeat; Ψ, adenovirus serotype 5 packaging signal.

    Journal: Scientific Reports

    Article Title: CRISPR/Cas9 delivery with one single adenoviral vector devoid of all viral genes

    doi: 10.1038/s41598-017-17180-w

    Figure Lengend Snippet: Plasmid toolbox for the construction of CRISPR/Cas9-HCAdV genomes. ( A ) Schematic presentation of intermediate CRISPR/Cas9 shuttle plasmids for simple gRNA manipulation and multiplexing and subsequent transfer of the customized CRISPR/Cas9 machinery into the HCAdV genome. Option 1: pShV-CBh-Cas9-gRNA for constitutive Cas9 expression. Option 2: pShV-TRE-Cas9-TeOn3G-gRNA for inducible Cas9 expression utilizing the TetOn3G system. Black arrowheads indicate unique restriction enzyme sites for insertion of further gRNA expression units. ( B ) Workflow for gRNA customization and multiplexing of the CRISPR/Cas9 machinery. Step1: Complementary annealed gRNA oligonucleotides are separately inserted between the Bsa I restriction enzyme sites resulting in pShV-CBh-Cas9-gRNA1, pShV-CBh-Cas9-gRNA2 and pShV-CBh-Cas9- gRNA3. Step 2: Customized gRNA expression units gRNA1 and gRNA2 are amplified by PCR using primers generating desired restriction enzyme sites. Step 3: gRNA1 and 2 are inserted into the respective restriction enzyme site within pShV-CBh-Cas9-gRNA1 resulting in pShV-CBh-Cas9-CBh-gRNA1-gRNA2-gRNA3. ( C ) Transfer of customized CRISPR/Cas9 transgenes into the HCAdV genomes. Option 1: Released CRISPR/Cas9 transgene cassettes flanked by homology arms are inserted into pHCAdV-HOM-CcdB-AMP-HOM replacing the CcdB-Amp R cassette. Option 2: Endonuclease guided cloning into pAd-FTC utilizing PI- Sce I and I- Ceu I. HOM, homology arms for homologous recombination into pHCAdV-HOM-CCBD-AMP-HOM; CBh-P, constitutive hybrid CMV enhancer/chicken β-actin promotor; TRE-P, inducible tetracycline responsible element promotor; TetOn3G, TetOn3G transactivator; Ef1-α-P, Ef1-α-Promotor; Cas9, Streptococcus pyogenes Cas9, gRNA, guide RNA expression unit; U6-P, U6 RNA polymerase III promotor, Kan R , Kanamycin resistance cassette; Amp R ; Ampicillin resistance cassette, Chl R , Chloramphenicol resistance cassette; CcdB, control of cell death B expression cassette; ITR, adenovirus serotype 5 inverted terminal repeat; Ψ, adenovirus serotype 5 packaging signal.

    Article Snippet: The shuttle plasmid backbone and the CBh-Cas9-gRNA expression cassette were separated on an agarose gel and the band containing the Cas9 and gRNA expression cassette(s) were gel-purified using the Wizard Gel- and PCR- clean-up kit (Promega).

    Techniques: Plasmid Preparation, CRISPR, Multiplexing, Expressing, Amplification, Polymerase Chain Reaction, Clone Assay, Homologous Recombination, RNA Expression

    Functionality tests of CRISPR-HCAdV in A549, HeLa and primary human skeletal myoblasts. Cells were transduced with different multiplicities of infection (MOI). Two days post-transduction genomic DNA was extracted. ( A ) A549 cells were transduced with HCAdV-Cbh-Cas9-gRNA-CCR5-88 or HCAdV-TRE-Cas9-Teton3G-gRNA-CCR5-88 and for cells that were transduced with HCAdV-TRE-Cas9-Teton3G-gRNA-CCR5-88 Cas9, expression was induced by doxycycline. Displayed is an agarose gel after T7E1 digest of PCR products of the amplified CCR5 locus. Arrowheads indicate specific cleavage products. ( B ) Hela cell transduced with HCAdV-EGFP-CBh-Cas9-gRNAHPV18E6. The HPV18 E6 locus was PCR-amplified and a T7E1 assay performed. Arrows show specific cleavage products ( C ) P rimary human skeletal myoblasts were transduced with HCAdV-CBh-Cas9-gRNACr1-Cr5. PCR products of amplified DMD locus revealed specific exon deletion. The border of the gels outside of the lanes was cropped. Note that the displayed gel in Fig. 2A was cropped from different parts of the gel and subsequently grouped. Activities are shown as percentages below the respective lanes.

    Journal: Scientific Reports

    Article Title: CRISPR/Cas9 delivery with one single adenoviral vector devoid of all viral genes

    doi: 10.1038/s41598-017-17180-w

    Figure Lengend Snippet: Functionality tests of CRISPR-HCAdV in A549, HeLa and primary human skeletal myoblasts. Cells were transduced with different multiplicities of infection (MOI). Two days post-transduction genomic DNA was extracted. ( A ) A549 cells were transduced with HCAdV-Cbh-Cas9-gRNA-CCR5-88 or HCAdV-TRE-Cas9-Teton3G-gRNA-CCR5-88 and for cells that were transduced with HCAdV-TRE-Cas9-Teton3G-gRNA-CCR5-88 Cas9, expression was induced by doxycycline. Displayed is an agarose gel after T7E1 digest of PCR products of the amplified CCR5 locus. Arrowheads indicate specific cleavage products. ( B ) Hela cell transduced with HCAdV-EGFP-CBh-Cas9-gRNAHPV18E6. The HPV18 E6 locus was PCR-amplified and a T7E1 assay performed. Arrows show specific cleavage products ( C ) P rimary human skeletal myoblasts were transduced with HCAdV-CBh-Cas9-gRNACr1-Cr5. PCR products of amplified DMD locus revealed specific exon deletion. The border of the gels outside of the lanes was cropped. Note that the displayed gel in Fig. 2A was cropped from different parts of the gel and subsequently grouped. Activities are shown as percentages below the respective lanes.

    Article Snippet: The shuttle plasmid backbone and the CBh-Cas9-gRNA expression cassette were separated on an agarose gel and the band containing the Cas9 and gRNA expression cassette(s) were gel-purified using the Wizard Gel- and PCR- clean-up kit (Promega).

    Techniques: CRISPR, Transduction, Infection, Expressing, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification