Journal: RSC Advances

Article Title: Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury

doi: 10.1039/d6ra03291e

Figure Lengend Snippet: YFP+ axon regeneration through nerve repair constructs. (A) Representative fluorescence images of YFP+ axons regenerating through either nerve grafts or PCL or PCL + GDNF conduits ( n = 6 per group). (B) Box and whisker plots show median, interquartile range (box), and the minimum and maximum values (whiskers) of total axon counts. (C) Axon counts were normalised to the proximal stump and expressed as a percentage (mean ± SD). Data were analysed with two-way ANOVA with Tukey's post hoc test p < 0.05 (*), p < 0.01 (**) (Scale bars = 200 µm).

Article Snippet: The endings were trimmed to create a gap of 3 mm between the proximal and distal stumps, and either a PCL or PCL + GDNF conduit was sutured end-to-end to the stumps using four 9–0 monofilament polyamide sutures (Ethilon®; Ethicon Ltd, England).

Techniques: Construct, Fluorescence, Whisker Assay

Journal: RSC Advances

Article Title: Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury

doi: 10.1039/d6ra03291e

Figure Lengend Snippet: β-III tubulin+ axon regeneration through nerve repair constructs. (A) Representative fluorescence images of TUBB3+ axons regenerating through either nerve grafts or PCL or PCL + GDNF conduits ( n = 6 per group). (B) Box and whisker plots show median, interquartile range (box), and the minimum and maximum values (whiskers) of total axon counts. (C) Axon counts were normalised to the proximal stump and expressed as a percentage (mean ± SD). Data were analysed with two-way ANOVA with Tukey's post hoc test. p < 0.01 (**) (Scale bars = 200 µm).

Article Snippet: The endings were trimmed to create a gap of 3 mm between the proximal and distal stumps, and either a PCL or PCL + GDNF conduit was sutured end-to-end to the stumps using four 9–0 monofilament polyamide sutures (Ethilon®; Ethicon Ltd, England).

Techniques: Construct, Fluorescence, Whisker Assay

Journal: RSC Advances

Article Title: Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury

doi: 10.1039/d6ra03291e

Figure Lengend Snippet: Schwann cell presence within nerve repair constructs. (A) Representative fluorescence images of S100B+ Schwann cells within nerve grafts or PCL or PCL + GDNF conduits. Cells migrated into fibrin hydrogel-filled spaces between nanofibrous mat folds. (B) S100B+ fluorescent area (µm 2 ) for Schwann cell quantification. (C) Fluorescent area was normalised to the entire measured area and expressed as a percentage (mean ± SD). Data were analysed by two-way ANOVA with Tukey's multiple comparisons test. Asterisks indicate significant differences between groups within the same segment ( p < 0.05 (*)), and all such comparisons are shown. Hash symbols ( p < 0.05 (#)) indicate significant differences between segments within the same group, and all such comparisons are shown, except that comparisons involving proximal segments are omitted. (Scale bars = 200 µm).

Article Snippet: The endings were trimmed to create a gap of 3 mm between the proximal and distal stumps, and either a PCL or PCL + GDNF conduit was sutured end-to-end to the stumps using four 9–0 monofilament polyamide sutures (Ethilon®; Ethicon Ltd, England).

Techniques: Construct, Fluorescence

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: The figures illustrate the docking interaction of paeoniflorin with the GDNF receptor (PDB ID: 1AGQ). (A) Shows a surface representation of the GDNF receptor, with the active site highlighted in yellow, where the ligand paeoniflorin (red) is bound. (B) 2D interaction diagram displays key interactions between paeoniflorin and the receptor, with hydrogen bonds and hydrophobic interactions clearly indicated. (C) Provides a 3D view of the docking, showing paeoniflorin (blue) interacting with GDNF, with hydrogen bonds represented by green dashed lines and key regions labeled. (D) Offers a zoomed-in view of the binding site, emphasizing hydrogen bonds and hydrophobic contacts, depicted within a ribbon structure for clarity.

Article Snippet: The ELISA kits for evaluating cellular and molecular targets included GDNF [E-EL-H1495; Elabscience GFRA1 [PKSH033670; Elabscience]; RET [AN00810P; Elabscience], AKT [E-EL-R0807 98T, Elabscience, Wuhan, China] ( ); ERK1/2 [E-AB-70292; Elabscience] ( ); and GSK3-Beta [KLR0989, KRISHGEN, Maharashtra, India] ( ).

Techniques: Labeling, Binding Assay

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: (A–H) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of cellular and molecular targets in MS rat model: GDNF (A) , GFRA1 (B) , AKT (C) , ERK1/2 (D) , GSK3-Beta (E) , in brain homogenates, and GDNF, GFRA1, AKT, ERK1/2, GSK3-Beta in CSF levels (F–H) . Statistical analysis was performed using a one-way ANOVA followed by Tukey’s post hoc test to determine significant differences among groups (A–H) . Data were presented as mean ± standard deviation (SD), with statistical significance set at p < 0.01. Each experimental group consisted of eight wistar rats (n = 8). β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–H) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Article Snippet: The ELISA kits for evaluating cellular and molecular targets included GDNF [E-EL-H1495; Elabscience GFRA1 [PKSH033670; Elabscience]; RET [AN00810P; Elabscience], AKT [E-EL-R0807 98T, Elabscience, Wuhan, China] ( ); ERK1/2 [E-AB-70292; Elabscience] ( ); and GSK3-Beta [KLR0989, KRISHGEN, Maharashtra, India] ( ).

Techniques: Standard Deviation, Control