rabbit anti gdh antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti gdh antibody
    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic <t>enzymes</t> <t>(LCAD,</t> <t>GDH,</t> AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
    Rabbit Anti Gdh Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gdh antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gdh antibody - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "Resveratrol mediates mitochondrial function through the sirtuin 3 pathway to improve abnormal metabolic remodeling in atrial fibrillation"

    Article Title: Resveratrol mediates mitochondrial function through the sirtuin 3 pathway to improve abnormal metabolic remodeling in atrial fibrillation

    Journal: European Journal of Histochemistry : EJH

    doi: 10.4081/ejh.2024.4004

    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic enzymes (LCAD, GDH, AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
    Figure Legend Snippet: Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic enzymes (LCAD, GDH, AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    anti gdh  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti gdh
    Anti Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gdh/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti gdh  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti gdh
    M2-EVs enhance glutamine metabolism (A) Bubble chart of the results of the metabolite analyses. (B) Pathway enrichment analysis of differentially modulated metabolites. (C) Detection of intracellular <t>GDH</t> activity and glutamate and αKG concentrations in OCPs treated with or without RANKL and/or M2-EVs (n = 3). (D) Western blot analysis of GDH <t>and</t> <t>GLS1</t> levels after M2-EVs treatment and semiquantitative analysis (n = 4). (E) Representative TEM images of the M2-EVs and M2-EVs without RNA. (F) Western blot and quantitative real-time PCR analysis of matrix metalloproteinase 9 (MMP9) expression under stimulation of M2-EVs and M2-EVs without RNA (n = 4). (G) Western blot analysis of the protein expression of OC or M2 marker genes in the presence or absence of the GDH inhibitor R162. (H and H') Representative TRAP staining and double-staining images showing the actin cytoskeleton in M2-EV-stimulated OCPs under various culture conditions and semiquantified (H') on day 7 (n = 3); scale bar, 100 μm (above) and 50 μm (below). (I) Quantitative real-time PCR analysis of the mRNA expression OC or M2 marker genes in the presence or absence of the GDH inhibitor R162 (n = 3). Student’s t test (C); One-way ANOVA with Tukey’s multiple comparisons test (C, D, F, H′, and I).
    Anti Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "M2 macrophages secrete glutamate-containing extracellular vesicles to alleviate osteoporosis by reshaping osteoclast precursor fate"

    Article Title: M2 macrophages secrete glutamate-containing extracellular vesicles to alleviate osteoporosis by reshaping osteoclast precursor fate

    Journal: Molecular Therapy

    doi: 10.1016/j.ymthe.2024.02.005

    M2-EVs enhance glutamine metabolism (A) Bubble chart of the results of the metabolite analyses. (B) Pathway enrichment analysis of differentially modulated metabolites. (C) Detection of intracellular GDH activity and glutamate and αKG concentrations in OCPs treated with or without RANKL and/or M2-EVs (n = 3). (D) Western blot analysis of GDH and GLS1 levels after M2-EVs treatment and semiquantitative analysis (n = 4). (E) Representative TEM images of the M2-EVs and M2-EVs without RNA. (F) Western blot and quantitative real-time PCR analysis of matrix metalloproteinase 9 (MMP9) expression under stimulation of M2-EVs and M2-EVs without RNA (n = 4). (G) Western blot analysis of the protein expression of OC or M2 marker genes in the presence or absence of the GDH inhibitor R162. (H and H') Representative TRAP staining and double-staining images showing the actin cytoskeleton in M2-EV-stimulated OCPs under various culture conditions and semiquantified (H') on day 7 (n = 3); scale bar, 100 μm (above) and 50 μm (below). (I) Quantitative real-time PCR analysis of the mRNA expression OC or M2 marker genes in the presence or absence of the GDH inhibitor R162 (n = 3). Student’s t test (C); One-way ANOVA with Tukey’s multiple comparisons test (C, D, F, H′, and I).
    Figure Legend Snippet: M2-EVs enhance glutamine metabolism (A) Bubble chart of the results of the metabolite analyses. (B) Pathway enrichment analysis of differentially modulated metabolites. (C) Detection of intracellular GDH activity and glutamate and αKG concentrations in OCPs treated with or without RANKL and/or M2-EVs (n = 3). (D) Western blot analysis of GDH and GLS1 levels after M2-EVs treatment and semiquantitative analysis (n = 4). (E) Representative TEM images of the M2-EVs and M2-EVs without RNA. (F) Western blot and quantitative real-time PCR analysis of matrix metalloproteinase 9 (MMP9) expression under stimulation of M2-EVs and M2-EVs without RNA (n = 4). (G) Western blot analysis of the protein expression of OC or M2 marker genes in the presence or absence of the GDH inhibitor R162. (H and H') Representative TRAP staining and double-staining images showing the actin cytoskeleton in M2-EV-stimulated OCPs under various culture conditions and semiquantified (H') on day 7 (n = 3); scale bar, 100 μm (above) and 50 μm (below). (I) Quantitative real-time PCR analysis of the mRNA expression OC or M2 marker genes in the presence or absence of the GDH inhibitor R162 (n = 3). Student’s t test (C); One-way ANOVA with Tukey’s multiple comparisons test (C, D, F, H′, and I).

    Techniques Used: Activity Assay, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Marker, Staining, Double Staining

    gdh  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc gdh
    Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdh/product/Cell Signaling Technology Inc
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    glutamate dehydrogenase gdh  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc glutamate dehydrogenase gdh
    Glutamate Dehydrogenase Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gdh  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc gdh
    Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdh/product/Cell Signaling Technology Inc
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    gdh  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gdh
    Exosomes improve mitochondrial transcription factor A (TFAM) expression and oxidative <t>phosphorylation</t> <t>(OXPHOS)</t> activity in DPSCs. (A) oxygen consumption rate (OCR) from seahorse analysis in DPSCs with supplementation of exosomes. (B) Basal respiration, ATP production, proton leak, maximal respiration and spare capacity in OCR assay. N = 5 independent experiments. (C) The volume of ATP in DPSCs with supplementation of exosomes. N = 4 independent experiments. (D) The protein expression of TFAM and five OXPHOS complexes by western blot analysis. (E) NADH/NAD of DPSCs with exosomes for 24, 48 and 72 h. N = 4 independent experiments. (F) Extracellular acidification rate (ECAR) from seahorse analysis in DPSCs with supplementation of exosomes. (G) Glycolytic reserve, glycolysis and glycolytic capacity in ECAR assay. N = 5 independent experiments. (H) The expression of <t>GDH,</t> Glut1 and CPT1A by western blot analysis. N = 3 independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001. Error bars are mean ± SD.
    Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Exosome‐shuttled mitochondrial transcription factor A mRNA promotes the osteogenesis of dental pulp stem cells through mitochondrial oxidative phosphorylation activation"

    Article Title: Exosome‐shuttled mitochondrial transcription factor A mRNA promotes the osteogenesis of dental pulp stem cells through mitochondrial oxidative phosphorylation activation

    Journal: Cell Proliferation

    doi: 10.1111/cpr.13324

    Exosomes improve mitochondrial transcription factor A (TFAM) expression and oxidative phosphorylation (OXPHOS) activity in DPSCs. (A) oxygen consumption rate (OCR) from seahorse analysis in DPSCs with supplementation of exosomes. (B) Basal respiration, ATP production, proton leak, maximal respiration and spare capacity in OCR assay. N = 5 independent experiments. (C) The volume of ATP in DPSCs with supplementation of exosomes. N = 4 independent experiments. (D) The protein expression of TFAM and five OXPHOS complexes by western blot analysis. (E) NADH/NAD of DPSCs with exosomes for 24, 48 and 72 h. N = 4 independent experiments. (F) Extracellular acidification rate (ECAR) from seahorse analysis in DPSCs with supplementation of exosomes. (G) Glycolytic reserve, glycolysis and glycolytic capacity in ECAR assay. N = 5 independent experiments. (H) The expression of GDH, Glut1 and CPT1A by western blot analysis. N = 3 independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001. Error bars are mean ± SD.
    Figure Legend Snippet: Exosomes improve mitochondrial transcription factor A (TFAM) expression and oxidative phosphorylation (OXPHOS) activity in DPSCs. (A) oxygen consumption rate (OCR) from seahorse analysis in DPSCs with supplementation of exosomes. (B) Basal respiration, ATP production, proton leak, maximal respiration and spare capacity in OCR assay. N = 5 independent experiments. (C) The volume of ATP in DPSCs with supplementation of exosomes. N = 4 independent experiments. (D) The protein expression of TFAM and five OXPHOS complexes by western blot analysis. (E) NADH/NAD of DPSCs with exosomes for 24, 48 and 72 h. N = 4 independent experiments. (F) Extracellular acidification rate (ECAR) from seahorse analysis in DPSCs with supplementation of exosomes. (G) Glycolytic reserve, glycolysis and glycolytic capacity in ECAR assay. N = 5 independent experiments. (H) The expression of GDH, Glut1 and CPT1A by western blot analysis. N = 3 independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001. Error bars are mean ± SD.

    Techniques Used: Expressing, Activity Assay, Western Blot, ECAR Assay

    gdh 1 2 12793s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gdh 1 2 12793s
    Gdh 1 2 12793s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gdh 1 2 12793s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gdh 1 2 12793s
    Gdh 1 2 12793s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gdh  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gdh
    Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit anti gdh antibody
    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic <t>enzymes</t> <t>(LCAD,</t> <t>GDH,</t> AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
    Rabbit Anti Gdh Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti gdh
    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic <t>enzymes</t> <t>(LCAD,</t> <t>GDH,</t> AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
    Anti Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc gdh
    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic <t>enzymes</t> <t>(LCAD,</t> <t>GDH,</t> AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
    Gdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc glutamate dehydrogenase gdh
    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic <t>enzymes</t> <t>(LCAD,</t> <t>GDH,</t> AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
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    Cell Signaling Technology Inc gdh 1 2 12793s
    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic <t>enzymes</t> <t>(LCAD,</t> <t>GDH,</t> AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.
    Gdh 1 2 12793s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic enzymes (LCAD, GDH, AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.

    Journal: European Journal of Histochemistry : EJH

    Article Title: Resveratrol mediates mitochondrial function through the sirtuin 3 pathway to improve abnormal metabolic remodeling in atrial fibrillation

    doi: 10.4081/ejh.2024.4004

    Figure Lengend Snippet: Resveratrol regulates the acetylation of metabolic enzymes through a SIRT3-dependent pathway during AF. A ) The mRNA expression level of SIRT3 in HL-1 cells was detected by RT-qPCR. B,C ) Western blot analysis was used to detect the protein expression level of SIRT3 in HL-1 cells and analyze its gray value. D,G ) Western blotting was used to detect the acetylated protein level and gray value analysis of key metabolic enzymes (LCAD, GDH, AceCS2) in HL-1 cells; * p <0.05, ** p <0.01, *** p <0.001; n=3.

    Article Snippet: The membrane was blocked with Tween-Tris buffered brine (TTBS) containing 5% skim milk at room temperature for 2 h and then incubated with the following primary antibodies: SIRT3 (C73E3) rabbit mAb (2627, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-LCAD antibody (2980, Cell Signaling Technology), rabbit anti-GDH antibody (12793, Cell Signaling Technology), rabbit anti-ACE2 antibody (92485, Cell Signaling Technology), and GAPDH (4970, Cell Signaling Technology).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot