gas liquid chromatography  (Thermo Fisher)


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  • 99
    Name:
    Shandon M 1 Embedding Matrix
    Description:
    Obtain optimal sample preparation with Thermo Scientific Shandon M 1 embedding matrix
    Catalog Number:
    1310ts
    Price:
    None
    Applications:
    Anatomical Pathology|Clinical
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher gas liquid chromatography
    Obtain optimal sample preparation with Thermo Scientific Shandon M 1 embedding matrix
    https://www.bioz.com/result/gas liquid chromatography/product/Thermo Fisher
    Average 99 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    gas liquid chromatography - by Bioz Stars, 2020-09
    99/100 stars

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    Gas Chromatography-Mass Spectrometry:

    Article Title: Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Downregulates ELOVL1 Gene Expression and Fatty Acid Synthesis in Goat Fetal Fibroblasts
    Article Snippet: .. Total lipids extraction with trichloromethane/methanol (v /v = 2:1) in an PBS-washed ampoule for GC-MS. After the transesterification with hydrochloric acid/methanol (v /v = 1:20) at 85 °C for 1 h, fatty acid methyl esters (FAME) were extracted with n-hexane at room temperature for 1 h and washed twice with water in preparation then separated in a gas chromatography-mass spectrum (Thermo TRACE 1310 GC-ISQ QD MS, Thermo Fisher Scientific Inc., 81 Wyman Street, Waltham, MA, USA, 02451) using a fused-silica capillary column with medium polarity (DB-5MS, 30 m × 0.25 mm × 0.25 μm, Agilent Technologies, Santa Clara, CA, USA). .. The initial capillary column chromatography program was set to column temperature 80 °C for 1 min, with ramping of 10 °C/min up to 200 °C, 5 °C/min up to 250 °C, 2 °C/min up to 270 °C, and a hold for 3 min.

    Article Title: Metabolite fingerprinting of pennycress (Thlaspi arvense L.) embryos to assess active pathways during oil synthesis
    Article Snippet: .. GC-MS analysis Alkylsilyl derivates were analysed using a Thermo Trace 1310 gas chromatograph coupled to an ISQ single quadrupole mass spectrometer. .. Alkylsilyl derivates were separated using a TG-5MS capillary (30 m×0.25 mm×0.50 μm) column from Thermo Scientific at a constant flow rate of 1.4ml min–1 .

    Article Title: GC-MS Screening Analysis for the Identification of Potential Migrants in Plastic and Paper-Based Candy Wrappers
    Article Snippet: .. Non-Targeted Analysis A Thermo Scientific Trace 1300 Series gas chromatograph (Thermo Fisher Scientific, San José, CA, USA) with a Trace ISQ LT mass detector (San José, CA, USA) (GC-MS) equipped with a Thermo Scientific AI 1310 automatic injector (San José, CA, USA) was used to carry out the analysis. .. The chromatographic conditions were as follows: a ZB-5MS (30 m × 0.25 mm × 0.25 µm) column from Phenomenex® (Torrance, CA, USA) was employed; the injector temperature was 300 °C.

    Chromatography:

    Article Title: Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Downregulates ELOVL1 Gene Expression and Fatty Acid Synthesis in Goat Fetal Fibroblasts
    Article Snippet: .. Total lipids extraction with trichloromethane/methanol (v /v = 2:1) in an PBS-washed ampoule for GC-MS. After the transesterification with hydrochloric acid/methanol (v /v = 1:20) at 85 °C for 1 h, fatty acid methyl esters (FAME) were extracted with n-hexane at room temperature for 1 h and washed twice with water in preparation then separated in a gas chromatography-mass spectrum (Thermo TRACE 1310 GC-ISQ QD MS, Thermo Fisher Scientific Inc., 81 Wyman Street, Waltham, MA, USA, 02451) using a fused-silica capillary column with medium polarity (DB-5MS, 30 m × 0.25 mm × 0.25 μm, Agilent Technologies, Santa Clara, CA, USA). .. The initial capillary column chromatography program was set to column temperature 80 °C for 1 min, with ramping of 10 °C/min up to 200 °C, 5 °C/min up to 250 °C, 2 °C/min up to 270 °C, and a hold for 3 min.

    Mass Spectrometry:

    Article Title: Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Downregulates ELOVL1 Gene Expression and Fatty Acid Synthesis in Goat Fetal Fibroblasts
    Article Snippet: .. Total lipids extraction with trichloromethane/methanol (v /v = 2:1) in an PBS-washed ampoule for GC-MS. After the transesterification with hydrochloric acid/methanol (v /v = 1:20) at 85 °C for 1 h, fatty acid methyl esters (FAME) were extracted with n-hexane at room temperature for 1 h and washed twice with water in preparation then separated in a gas chromatography-mass spectrum (Thermo TRACE 1310 GC-ISQ QD MS, Thermo Fisher Scientific Inc., 81 Wyman Street, Waltham, MA, USA, 02451) using a fused-silica capillary column with medium polarity (DB-5MS, 30 m × 0.25 mm × 0.25 μm, Agilent Technologies, Santa Clara, CA, USA). .. The initial capillary column chromatography program was set to column temperature 80 °C for 1 min, with ramping of 10 °C/min up to 200 °C, 5 °C/min up to 250 °C, 2 °C/min up to 270 °C, and a hold for 3 min.

    Article Title: Metabolite fingerprinting of pennycress (Thlaspi arvense L.) embryos to assess active pathways during oil synthesis
    Article Snippet: .. GC-MS analysis Alkylsilyl derivates were analysed using a Thermo Trace 1310 gas chromatograph coupled to an ISQ single quadrupole mass spectrometer. .. Alkylsilyl derivates were separated using a TG-5MS capillary (30 m×0.25 mm×0.50 μm) column from Thermo Scientific at a constant flow rate of 1.4ml min–1 .

    Article Title: Simultaneous Determination of 18 Polycyclic Aromatic Hydrocarbons in Daily Foods (Hanoi Metropolitan Area) by Gas Chromatography–Tandem Mass Spectrometry
    Article Snippet: .. GC–MS/MS analyses were performed on a Thermo Fisher Scientific (Waltham, MA, USA) system consisting of a Trace GC 1310 gas chromatograph, a TriPlus RSH Autosampler, and TSQ 8000 mass spectrometer (Thermo, Waltham, MA, USA). .. The TraceFinder software from Thermo Fisher Scientific was used for data processing.

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    Thermo Fisher mitotracker red staining
    Metabolic alterations induced by heme oxygenase-1 inhibitors in thyroid cancer cells. The expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) was determined following treatment with zinc protoporphyrin-IX (ZnPP), ketoconazole (Keto), or vehicle control ( A ). Corresponding changes in oxygen consumption rates (OCR) and <t>MitoTracker</t> staining were evaluated in FTC-133 ( B ) and 8505C ( C ) cells. *** p
    Mitotracker Red Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher l 1 13 c leucine enrichments
    Postexercise myofibrillar protein fractional synthetic rates assessed using  l -[1- 13 C]-leucine after ingestion of PLA, 15G, 30G, or 45G in older men. Values represent means ± SEMs,  n  = 12. Data were analyzed with 1-factor ANOVA. Tukey post hoc testing was used to detect differences between groups. Labeled means without a common letter differ ( P 
    L 1 13 C Leucine Enrichments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher rarres1 expression
    Effects of <t>RARRES1</t> depleted vs. RARRES1-expressing epithelial cells on lipid metabolism. Unlike RARRES1 expressing cells, RARRES1 depleted cells increase de novo lipogenesis. This effect enables cells to improve mitochondrial respiration during starvation due to an increase in endogenous fatty acid availability. In RARRES1 expressing epithelial cells, glucose is mostly directed to lactate production in normal conditions. During serum starvation expression of RARRES1 increases and exasperates the lipid availability for fatty acid oxidation. ETC: Electron transport chain; TCA: tricarboxylic acid cycle; DHA: docosahexaenoic Acid.
    Rarres1 Expression, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 2 article reviews
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    85
    Thermo Fisher database library nist07
    Chromatogram showing peaks of myo -inositol (RT- 16.56) and respective mass fragments as observed by GC/MS analysis. ( a ) Non-transgenic and ( b ) T 3 transgenic seeds of 196-11-6. The peak corresponding to myo -inositol has been marked by an arrow. Myo -inositol was quantified as a hexa-trimethylsilyl ether derivative and was identified by comparing the mass fragmentation pattern with the database library <t>NIST07.</t>
    Database Library Nist07, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    database library nist07 - by Bioz Stars, 2020-09
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    Image Search Results


    Metabolic alterations induced by heme oxygenase-1 inhibitors in thyroid cancer cells. The expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) was determined following treatment with zinc protoporphyrin-IX (ZnPP), ketoconazole (Keto), or vehicle control ( A ). Corresponding changes in oxygen consumption rates (OCR) and MitoTracker staining were evaluated in FTC-133 ( B ) and 8505C ( C ) cells. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Heme Oxygenase-1 Inhibitors Induce Cell Cycle Arrest and Suppress Tumor Growth in Thyroid Cancer Cells

    doi: 10.3390/ijms19092502

    Figure Lengend Snippet: Metabolic alterations induced by heme oxygenase-1 inhibitors in thyroid cancer cells. The expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) was determined following treatment with zinc protoporphyrin-IX (ZnPP), ketoconazole (Keto), or vehicle control ( A ). Corresponding changes in oxygen consumption rates (OCR) and MitoTracker staining were evaluated in FTC-133 ( B ) and 8505C ( C ) cells. *** p

    Article Snippet: MitoTracker Red Staining Following treatment with ZnPP, ketoconazole, or vehicle control for 48 h, cells were fixed and stained with MitoTracker Red CMXRos (Thermo Fisher Scientific) for 30 min. DAPI counterstaining was performed to visualize the nuclei.

    Techniques: Expressing, Pyrolysis Gas Chromatography, Staining

    Postexercise myofibrillar protein fractional synthetic rates assessed using  l -[1- 13 C]-leucine after ingestion of PLA, 15G, 30G, or 45G in older men. Values represent means ± SEMs,  n  = 12. Data were analyzed with 1-factor ANOVA. Tukey post hoc testing was used to detect differences between groups. Labeled means without a common letter differ ( P 

    Journal: The Journal of Nutrition

    Article Title: Dose-Dependent Increases in Whole-Body Net Protein Balance and Dietary Protein-Derived Amino Acid Incorporation into Myofibrillar Protein During Recovery from Resistance Exercise in Older Men

    doi: 10.1093/jn/nxy263

    Figure Lengend Snippet: Postexercise myofibrillar protein fractional synthetic rates assessed using l -[1- 13 C]-leucine after ingestion of PLA, 15G, 30G, or 45G in older men. Values represent means ± SEMs, n  = 12. Data were analyzed with 1-factor ANOVA. Tukey post hoc testing was used to detect differences between groups. Labeled means without a common letter differ ( P 

    Article Snippet: Myofibrillar protein-bound l -[ring -2 H5 ]-phenylalanine enrichments were determined by GC-MS analysis, whereas the l -[1-13 C]-phenylalanine and l -[1-13 C]-leucine enrichments were determined by GC-C-isotope ratio mass spectrometer analysis (Trace GC Ultra, IRMS model MAT 253; Thermo Scientific).

    Techniques: Proximity Ligation Assay, Labeling

    l -[1- 13 C]-phenylalanine incorporation into myofibrillar protein after ingestion of 15G, 30G, or 45G after resistance exercise in older men. Values represent means ± SEMs,  n  = 12. Data were analyzed with a 1-factor ANOVA. Tukey post hoc testing was used to detect differences between groups. Labeled means without a common letter differ ( P 

    Journal: The Journal of Nutrition

    Article Title: Dose-Dependent Increases in Whole-Body Net Protein Balance and Dietary Protein-Derived Amino Acid Incorporation into Myofibrillar Protein During Recovery from Resistance Exercise in Older Men

    doi: 10.1093/jn/nxy263

    Figure Lengend Snippet: l -[1- 13 C]-phenylalanine incorporation into myofibrillar protein after ingestion of 15G, 30G, or 45G after resistance exercise in older men. Values represent means ± SEMs, n  = 12. Data were analyzed with a 1-factor ANOVA. Tukey post hoc testing was used to detect differences between groups. Labeled means without a common letter differ ( P 

    Article Snippet: Myofibrillar protein-bound l -[ring -2 H5 ]-phenylalanine enrichments were determined by GC-MS analysis, whereas the l -[1-13 C]-phenylalanine and l -[1-13 C]-leucine enrichments were determined by GC-C-isotope ratio mass spectrometer analysis (Trace GC Ultra, IRMS model MAT 253; Thermo Scientific).

    Techniques: Labeling

    Plasma  l -[1- 13 C]-phenylalanine (A),  l -[ ring - 2 H 5 ]-phenylalanine (B), and  l -[1- 13 C]-leucine (C) enrichments after ingestion of PLA, 15G, 30G, or 45G after resistance exercise in older men. The dotted line represents the ingestion of the beverage. Values represent means ± SEMs,  n  = 12. Data were analyzed with repeated measures (time × treatment group) ANOVA and separate analyses when a significant interaction was detected (see Methods section).  a Different from PLA at that time ( P 

    Journal: The Journal of Nutrition

    Article Title: Dose-Dependent Increases in Whole-Body Net Protein Balance and Dietary Protein-Derived Amino Acid Incorporation into Myofibrillar Protein During Recovery from Resistance Exercise in Older Men

    doi: 10.1093/jn/nxy263

    Figure Lengend Snippet: Plasma l -[1- 13 C]-phenylalanine (A), l -[ ring - 2 H 5 ]-phenylalanine (B), and l -[1- 13 C]-leucine (C) enrichments after ingestion of PLA, 15G, 30G, or 45G after resistance exercise in older men. The dotted line represents the ingestion of the beverage. Values represent means ± SEMs, n  = 12. Data were analyzed with repeated measures (time × treatment group) ANOVA and separate analyses when a significant interaction was detected (see Methods section). a Different from PLA at that time ( P 

    Article Snippet: Myofibrillar protein-bound l -[ring -2 H5 ]-phenylalanine enrichments were determined by GC-MS analysis, whereas the l -[1-13 C]-phenylalanine and l -[1-13 C]-leucine enrichments were determined by GC-C-isotope ratio mass spectrometer analysis (Trace GC Ultra, IRMS model MAT 253; Thermo Scientific).

    Techniques: Proximity Ligation Assay

    Effects of RARRES1 depleted vs. RARRES1-expressing epithelial cells on lipid metabolism. Unlike RARRES1 expressing cells, RARRES1 depleted cells increase de novo lipogenesis. This effect enables cells to improve mitochondrial respiration during starvation due to an increase in endogenous fatty acid availability. In RARRES1 expressing epithelial cells, glucose is mostly directed to lactate production in normal conditions. During serum starvation expression of RARRES1 increases and exasperates the lipid availability for fatty acid oxidation. ETC: Electron transport chain; TCA: tricarboxylic acid cycle; DHA: docosahexaenoic Acid.

    Journal: PLoS ONE

    Article Title: Tumor suppressor RARRES1- A novel regulator of fatty acid metabolism in epithelial cells

    doi: 10.1371/journal.pone.0208756

    Figure Lengend Snippet: Effects of RARRES1 depleted vs. RARRES1-expressing epithelial cells on lipid metabolism. Unlike RARRES1 expressing cells, RARRES1 depleted cells increase de novo lipogenesis. This effect enables cells to improve mitochondrial respiration during starvation due to an increase in endogenous fatty acid availability. In RARRES1 expressing epithelial cells, glucose is mostly directed to lactate production in normal conditions. During serum starvation expression of RARRES1 increases and exasperates the lipid availability for fatty acid oxidation. ETC: Electron transport chain; TCA: tricarboxylic acid cycle; DHA: docosahexaenoic Acid.

    Article Snippet: The cDNA samples were used to quantify RARRES1 expression using the fast real-time PCR kit (Thermo Fisher) with the appropriate TaqMan probes (Thermo Fisher: RARRES1 Hs00894859_m1 and 18s Hs03003631_g1) in a StepOne (Applied Biosystems) compact qPCR machine.

    Techniques: Expressing

    RARRES1 regulates DNL. (A) Citrate levels in stable RARRES1 knockdown MCF10A cells were calculated using MS/MS method and compared to empty vector MCF10A cells. (B) Glycolytic activity was assessed in MCF 10A and PWR-1E cells. The glycolytic reserve was calculated for both cell lines. (C) Transient RARRES1 KD and scramble control were treated with C75 inhibitor and glycolytic activity was assessed. Glycolytic usage and reserve were assessed in vehicle and C75 treated groups. (D) Scrambled siRNA or RARRES1 siRNA transfected cells were treated with vehicle or 40 μM C75 for 2 hours or 4 hours. Cells were then stained with Oil Red O and DAPI. (E) A schematic diagram of glycolysis and glucose dependent de novo lipogenesis pathway in RARES1 depleted epithelial cells and the effect of C75 on these cells is depicted. G: Glucose; O: Oligomycin; 2-DG: 2-Deoxy-D-glucose.

    Journal: PLoS ONE

    Article Title: Tumor suppressor RARRES1- A novel regulator of fatty acid metabolism in epithelial cells

    doi: 10.1371/journal.pone.0208756

    Figure Lengend Snippet: RARRES1 regulates DNL. (A) Citrate levels in stable RARRES1 knockdown MCF10A cells were calculated using MS/MS method and compared to empty vector MCF10A cells. (B) Glycolytic activity was assessed in MCF 10A and PWR-1E cells. The glycolytic reserve was calculated for both cell lines. (C) Transient RARRES1 KD and scramble control were treated with C75 inhibitor and glycolytic activity was assessed. Glycolytic usage and reserve were assessed in vehicle and C75 treated groups. (D) Scrambled siRNA or RARRES1 siRNA transfected cells were treated with vehicle or 40 μM C75 for 2 hours or 4 hours. Cells were then stained with Oil Red O and DAPI. (E) A schematic diagram of glycolysis and glucose dependent de novo lipogenesis pathway in RARES1 depleted epithelial cells and the effect of C75 on these cells is depicted. G: Glucose; O: Oligomycin; 2-DG: 2-Deoxy-D-glucose.

    Article Snippet: The cDNA samples were used to quantify RARRES1 expression using the fast real-time PCR kit (Thermo Fisher) with the appropriate TaqMan probes (Thermo Fisher: RARRES1 Hs00894859_m1 and 18s Hs03003631_g1) in a StepOne (Applied Biosystems) compact qPCR machine.

    Techniques: Mass Spectrometry, Plasmid Preparation, Activity Assay, Transfection, Staining

    RARRES1 depletion regulates fatty acid oxidation. (A) ATP content was quantified in transient RARRES1 KD MCF 10A cells and scrambled siRNA MCF 10A cells after 2 and 3 hours of 40 μg/mL C75 treatment. (B) All detected Acylcarnitines, AC-4:0 and AC-16:0, were quantified and validated in the non-targeted LC-MS results of RARRES1 siRNA transfected MCF 10A cells ( S2A Fig ) . The fold change was normalized to scrambled siRNA transfected MCF 10A cells. Fatty acid oxidation rate in RARRES1-depleted cells was measured in nutrient rich media. FAO dependency and flexibility of the cells were calculated. Initial inhibition of FAO by etomoxir measures how dependent the cells are on that particular fuel source to meet the energy demand. Using a combination of glycolytic, glutaminolytic and FAO inhibitors, the cells' capacity and flexibility in meeting energy demand were calculated in terms of oxygen consumption rate. (C) Fatty acid oxidation dependent mitochondrial respiration was quantified and the effects of exogenous fatty acids (palmitate treatment) on FAO-dependent OCR in RARRES1-depleted and scrambled siRNA MCF 10A cells in glucose and serum starved media. (D) MCF 10A were starved and treated with etomoxir to measure fatty acid oxidation rate dependent on endogenous fatty acids. Scrambled siRNA and transient RARRES1 siRNA were measured and compared. Basal respiration and ATP production were quantified.

    Journal: PLoS ONE

    Article Title: Tumor suppressor RARRES1- A novel regulator of fatty acid metabolism in epithelial cells

    doi: 10.1371/journal.pone.0208756

    Figure Lengend Snippet: RARRES1 depletion regulates fatty acid oxidation. (A) ATP content was quantified in transient RARRES1 KD MCF 10A cells and scrambled siRNA MCF 10A cells after 2 and 3 hours of 40 μg/mL C75 treatment. (B) All detected Acylcarnitines, AC-4:0 and AC-16:0, were quantified and validated in the non-targeted LC-MS results of RARRES1 siRNA transfected MCF 10A cells ( S2A Fig ) . The fold change was normalized to scrambled siRNA transfected MCF 10A cells. Fatty acid oxidation rate in RARRES1-depleted cells was measured in nutrient rich media. FAO dependency and flexibility of the cells were calculated. Initial inhibition of FAO by etomoxir measures how dependent the cells are on that particular fuel source to meet the energy demand. Using a combination of glycolytic, glutaminolytic and FAO inhibitors, the cells' capacity and flexibility in meeting energy demand were calculated in terms of oxygen consumption rate. (C) Fatty acid oxidation dependent mitochondrial respiration was quantified and the effects of exogenous fatty acids (palmitate treatment) on FAO-dependent OCR in RARRES1-depleted and scrambled siRNA MCF 10A cells in glucose and serum starved media. (D) MCF 10A were starved and treated with etomoxir to measure fatty acid oxidation rate dependent on endogenous fatty acids. Scrambled siRNA and transient RARRES1 siRNA were measured and compared. Basal respiration and ATP production were quantified.

    Article Snippet: The cDNA samples were used to quantify RARRES1 expression using the fast real-time PCR kit (Thermo Fisher) with the appropriate TaqMan probes (Thermo Fisher: RARRES1 Hs00894859_m1 and 18s Hs03003631_g1) in a StepOne (Applied Biosystems) compact qPCR machine.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Transfection, Inhibition

    RARRES1 regulates lipid accumulation. (A) Classes of metabolites measured by non-targeted LC-MS analysis of transient RARRES1 knockdown MCF 10A cells and scrambled control cells. (B) GC-MS was run in transient RARRES1 knockdown MCF 10A cells and monounsaturated fatty acids were analyzed. Oleic acid is represented in the bar graph. (C) RARRES1 was transiently knocked down in MCF10A cells and Oil Red O staining was used to stain for lipid accumulation. The bar graph represents the intensity of lipid droplet staining. Three biological replicates are represented in the bar graph. (D) RARRES1-YFP was exogenously expressed and the transfected cells were treated with oleic acid and stained with Oil Red O to localize lipid droplets. The numbers of cells with YFP or RARRES1-YFP transfection displaying lipid droplets or no lipid droplets were counted in both HEK 293T cells and MCF 10A cells. The y-axis represents the % total of cells with lipid droplet formation (blue) and % cells with no lipid droplet formation (red). (E) MCF10A cells were serum starved for 18 hours or 40 hours. qPCR analysis was subsequently done. 18S was used as the endogenous control. (F) A schematic representation of metabolites that were upregulated in RARRES1-depleted cells.

    Journal: PLoS ONE

    Article Title: Tumor suppressor RARRES1- A novel regulator of fatty acid metabolism in epithelial cells

    doi: 10.1371/journal.pone.0208756

    Figure Lengend Snippet: RARRES1 regulates lipid accumulation. (A) Classes of metabolites measured by non-targeted LC-MS analysis of transient RARRES1 knockdown MCF 10A cells and scrambled control cells. (B) GC-MS was run in transient RARRES1 knockdown MCF 10A cells and monounsaturated fatty acids were analyzed. Oleic acid is represented in the bar graph. (C) RARRES1 was transiently knocked down in MCF10A cells and Oil Red O staining was used to stain for lipid accumulation. The bar graph represents the intensity of lipid droplet staining. Three biological replicates are represented in the bar graph. (D) RARRES1-YFP was exogenously expressed and the transfected cells were treated with oleic acid and stained with Oil Red O to localize lipid droplets. The numbers of cells with YFP or RARRES1-YFP transfection displaying lipid droplets or no lipid droplets were counted in both HEK 293T cells and MCF 10A cells. The y-axis represents the % total of cells with lipid droplet formation (blue) and % cells with no lipid droplet formation (red). (E) MCF10A cells were serum starved for 18 hours or 40 hours. qPCR analysis was subsequently done. 18S was used as the endogenous control. (F) A schematic representation of metabolites that were upregulated in RARRES1-depleted cells.

    Article Snippet: The cDNA samples were used to quantify RARRES1 expression using the fast real-time PCR kit (Thermo Fisher) with the appropriate TaqMan probes (Thermo Fisher: RARRES1 Hs00894859_m1 and 18s Hs03003631_g1) in a StepOne (Applied Biosystems) compact qPCR machine.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Gas Chromatography-Mass Spectrometry, Staining, Transfection, Real-time Polymerase Chain Reaction

    Differential expression of RARRES1 in cancers correlates with expression of fatty acid metabolism genes. Cancers where RARRES1 gene was differentially expressed were chosen and correlative analysis was done. FASN , SCD , PPARG , SREBF1 , PPARGC1A and PPARA , were chosen as candidate genes important in fatty acid oxidation and lipogenesis. (A) Three subtypes of breast cancer, 5 types of colorectal cancer and metastatic versus primary sites of prostate cancer were analyzed and fold change difference (with p-value

    Journal: PLoS ONE

    Article Title: Tumor suppressor RARRES1- A novel regulator of fatty acid metabolism in epithelial cells

    doi: 10.1371/journal.pone.0208756

    Figure Lengend Snippet: Differential expression of RARRES1 in cancers correlates with expression of fatty acid metabolism genes. Cancers where RARRES1 gene was differentially expressed were chosen and correlative analysis was done. FASN , SCD , PPARG , SREBF1 , PPARGC1A and PPARA , were chosen as candidate genes important in fatty acid oxidation and lipogenesis. (A) Three subtypes of breast cancer, 5 types of colorectal cancer and metastatic versus primary sites of prostate cancer were analyzed and fold change difference (with p-value

    Article Snippet: The cDNA samples were used to quantify RARRES1 expression using the fast real-time PCR kit (Thermo Fisher) with the appropriate TaqMan probes (Thermo Fisher: RARRES1 Hs00894859_m1 and 18s Hs03003631_g1) in a StepOne (Applied Biosystems) compact qPCR machine.

    Techniques: Expressing

    Chromatogram showing peaks of myo -inositol (RT- 16.56) and respective mass fragments as observed by GC/MS analysis. ( a ) Non-transgenic and ( b ) T 3 transgenic seeds of 196-11-6. The peak corresponding to myo -inositol has been marked by an arrow. Myo -inositol was quantified as a hexa-trimethylsilyl ether derivative and was identified by comparing the mass fragmentation pattern with the database library NIST07.

    Journal: Rice

    Article Title: RNAi mediated down regulation of myo-inositol-3-phosphate synthase to generate low phytate rice

    doi: 10.1186/1939-8433-6-12

    Figure Lengend Snippet: Chromatogram showing peaks of myo -inositol (RT- 16.56) and respective mass fragments as observed by GC/MS analysis. ( a ) Non-transgenic and ( b ) T 3 transgenic seeds of 196-11-6. The peak corresponding to myo -inositol has been marked by an arrow. Myo -inositol was quantified as a hexa-trimethylsilyl ether derivative and was identified by comparing the mass fragmentation pattern with the database library NIST07.

    Article Snippet: Myo -inositol hexa-trimethylsilyl ether was identified by comparing the mass fragmentation pattern with the database library NIST07 (MS Library Software, Thermo Scientific).

    Techniques: Gas Chromatography-Mass Spectrometry, Transgenic Assay