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Hewlett-Packard gas chromatography
Gas Chromatography, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 94/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Effects of Antibiotics on Bacterial Species Composition and Metabolic Activities in Chemostats Containing Defined Populations of Human Gut Microorganisms
Article Snippet: Bacterial identification by gas chromatography of whole cell fatty acids , p 228–241 Hewlett-Packard application note.

Article Title: Liquid Phase Hydrogenation of Pharmaceutical Interest Nitroarenes over Gold-Supported Alumina Nanowires Catalysts
Article Snippet: Products were identified by gas chromatography using a GC Hewlett Packard HP 4890 D gas chromatograph (Hewlett Packard HP-4890, Hewlett Packard, Palo Alto, CA, USA) equipped with an HP-5 capillary column and a flame-ionization detector.

Article Title: Essential Oil of Betula pendula Roth. Buds
Article Snippet: Gas chromatography The oils were analyzed by GC using a Hewlett Packard 6890 system.

Article Title: Ligand-Enhanced Abiotic Iron Oxidation and the Effects of Chemical versus Biological Iron Cycling in Anoxic Environments
Article Snippet: The evolution of N2 O in abiotic reactions was assessed qualitatively by gas-chromatography using a Hewlett-Packard 5890 Series II Plus Gas Chromatograph equipped with a Thermal Conductivity Detector.

Article Title: Increased Polyamine Biosynthesis Enhances Stress Tolerance by Preventing the Accumulation of Reactive Oxygen Species: T-DNA Mutational Analysis of Oryza sativa Lysine Decarboxylase-like Protein 1
Article Snippet: Tissue was sealed in 20 ml vials for 1 h and then ethylene content was determined in 1 ml of headspace gas by gas chromatography using an activated alumina column at 250°C and a flame ionization detector (Hewlett Packard 5890 Series II, USA).

Article Title: Risk Assessment on Benzene Exposure among Gasoline Station Workers
Article Snippet: Benzene concentration was analyzed by gas chromatography with flame ionization detector (GC-FID) (Hewlett Packard 1996, Germany).

Article Title: Gut microbiota composition during infancy and subsequent behavioural outcomes
Article Snippet: Additional aliquots of faecal samples were transported on dry ice to the Commonwealth Science and Industrial Research Organisation laboratories, Adelaide, Australia, where short-chain fatty acids (SCFAs) were quantified by capillary gas chromatography (GC; 5890 series II Hewlett Packard, Australia).

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    Hewlett-Packard strain mm1ida2h 1
    Biosurfactant production by Cobetia sp. strain <t>MM1IDA2H-1</t> in presence of DBT or microbial competitor. The production of surface-active compounds was evaluated by surface tension measures on cell-free supernatants samples obtained during growth in: (A) minimal media containing DBT as the sole carbon source and; (B) minimal media supplemented with succinate 30 mM and containing inactivated cells of the competitor A. salmonicida . Error bars indicate standard deviation ( n = 3).
    Strain Mm1ida2h 1, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Hewlett-Packard 3αhp
    Examples of MDA-MB-231 tumors and tumor histology from 5αP- and <t>3αHP-treated</t> mice . (A) Large aggressive tumor from a 5αP-treated mouse, with histologic sections showing (B) invasion of rib-cage muscle by the spreading tumor cells, and (C) higher magnification of region with numerous mitoses (arrows). (D) Residual tumor from a 3αHP-treated mouse, with no signs of invasion (E) and showing region of tumor with numerous apoptotic and necrotic cells (F) . Formalin-fixed sections (5 μm) stained with hematoxylin and eosin. Scale bars at 1.0 cm for whole tumors (A, D), at 160 µm for (B) and (E), and at 40 µm for (C) and (F).
    3αhp, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Hewlett-Packard bioactive fractions
    2D confocal imaging: a qualitative analysis. Confocal image stacks of 72-h S. mutans UA 159 biofilms following topical treatment with (a) A. gratissima : fraction Ag 4 ; (b) B. dracunculifolia : fraction Bd 2 ; (c) C. sativum : fraction Cs 4 ; (d) L. sidoides : fraction Ls 3 ; and (e) vehicle (propylene glycol, 6.25% v/v). The structures depicted in red (Dextran, Alexa Fluor 6) represent the extracellular polysaccharides that constitute the biofilm matrix, while the structures depicted in green (Syto 9) are metabolically active bacterial cells (optical magnitude 63x). It can be noted that all <t>bioactive</t> fractions ((a)–(d)) affected the EPS matrix making it less intimately interspersed between and over the cells than did the vehicle alone (e).
    Bioactive Fractions, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Hewlett-Packard cytotoxicity against caki 1
    Synergistic mechanisms of anticancer activities induced by MC ‐4 and everolimus alone and in combination. A, Effect of MC ‐4 on the anticancer signaling pathways in RCC cells. <t>Caki‐1</t> and 786‐O cells were treated with MC ‐4 for 24 h, and then Western blotting was performed. Representative blots are shown. B, Representative photomicrographs of immunohistochemical ( IHC ) analysis of PKM 2 (bottom) in the indicated tumors from mice treated with MC ‐4 or vehicle (lower panel). C, Combination effects of MC ‐4 and everolimus on cell viability. RCC cell lines (Caki‐1 and 786‐O) were treated for 72 h with MC ‐4 in combination with everolimus. Cell viability was determined by MTT assay and is expressed relative to the value without treatment. The data are means ± SEM from three independent experiments. ** P
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    Biosurfactant production by Cobetia sp. strain MM1IDA2H-1 in presence of DBT or microbial competitor. The production of surface-active compounds was evaluated by surface tension measures on cell-free supernatants samples obtained during growth in: (A) minimal media containing DBT as the sole carbon source and; (B) minimal media supplemented with succinate 30 mM and containing inactivated cells of the competitor A. salmonicida . Error bars indicate standard deviation ( n = 3).

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: Biosurfactant production by Cobetia sp. strain MM1IDA2H-1 in presence of DBT or microbial competitor. The production of surface-active compounds was evaluated by surface tension measures on cell-free supernatants samples obtained during growth in: (A) minimal media containing DBT as the sole carbon source and; (B) minimal media supplemented with succinate 30 mM and containing inactivated cells of the competitor A. salmonicida . Error bars indicate standard deviation ( n = 3).

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Standard Deviation

    Inhibition of quorum-sensing-dependent phenotypes by the biosurfactant produced using the Cobetia sp. strain MM1IDA2H-1.A. Purple phenotype response of Chromobacterium violaceum ATCC 12472 to different concentrations (mg ml −1 ) of biosurfactant (BS) produced by the Cobetia sp. strain MM1IDA2H-1. The production of the purple pigment violacein is under quorum sensing control in C. violaceum , therefore, the loss of this phenotype in the presence of BS was associated with the inhibition of quorum sensing. At the evaluated concentrations no effect on growth was observed.B. Interaction of quorum sensing signals with the biosurfactant. HSLs enriched cell-free supernatants of A. salmonicida were mixed with the biosurfactant and used to induce the quorum sensing pigmented phenotype in the HSL not producer strain CV026. 1: C. violaceum ; 2: CV026 exposed to C. violaceum cell-free supernatant; 3: CV026 exposed to A. salmonicida cell-free supernatant; 4: strain CV026 unexposed; 5: CV026 exposed to A. salmonicida cell-free supernatant mixed with biosurfactant; 6: CV026 exposed to C. violaceum cell-free supernatant mixed with biosurfactant; 7: A. tumefaciens exposed to A. salmonicida cell-free supernatant.C. Surface tension (ST) of water at different concentrations (mg l −1 in logarithmic scale) of the BS, and V. anguillarum biofilm formation at different concentrations (mg l −1 in logarithmic scale). The CMC was established when reduction of ST was stabilized without changes (at 80 mg l −1 ). Values for ST represent the average of three independent assays.

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: Inhibition of quorum-sensing-dependent phenotypes by the biosurfactant produced using the Cobetia sp. strain MM1IDA2H-1.A. Purple phenotype response of Chromobacterium violaceum ATCC 12472 to different concentrations (mg ml −1 ) of biosurfactant (BS) produced by the Cobetia sp. strain MM1IDA2H-1. The production of the purple pigment violacein is under quorum sensing control in C. violaceum , therefore, the loss of this phenotype in the presence of BS was associated with the inhibition of quorum sensing. At the evaluated concentrations no effect on growth was observed.B. Interaction of quorum sensing signals with the biosurfactant. HSLs enriched cell-free supernatants of A. salmonicida were mixed with the biosurfactant and used to induce the quorum sensing pigmented phenotype in the HSL not producer strain CV026. 1: C. violaceum ; 2: CV026 exposed to C. violaceum cell-free supernatant; 3: CV026 exposed to A. salmonicida cell-free supernatant; 4: strain CV026 unexposed; 5: CV026 exposed to A. salmonicida cell-free supernatant mixed with biosurfactant; 6: CV026 exposed to C. violaceum cell-free supernatant mixed with biosurfactant; 7: A. tumefaciens exposed to A. salmonicida cell-free supernatant.C. Surface tension (ST) of water at different concentrations (mg l −1 in logarithmic scale) of the BS, and V. anguillarum biofilm formation at different concentrations (mg l −1 in logarithmic scale). The CMC was established when reduction of ST was stabilized without changes (at 80 mg l −1 ). Values for ST represent the average of three independent assays.

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Inhibition, Produced

    Microscopy of diffusible lipid structures produced by Cobetia sp. strain MM1IDA2H-1. Panels (A) and (C) are respectively TEM and epifluorescence images of cells growing on Bushnell-Hass supplemented with succinate 30 mM. Panels (B) and (D) are respectively TEM and epifluorescence images of cells growing with DBT as the only carbon source. For epifluorescence microscopy Cobetia sp. strain MM1IDA2H-1 cells were stained with BODIPY 505/515 and SYTO 9 to detect respectively lipidic structures and (nucleic acid) cells (red bar = 10 μm).

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: Microscopy of diffusible lipid structures produced by Cobetia sp. strain MM1IDA2H-1. Panels (A) and (C) are respectively TEM and epifluorescence images of cells growing on Bushnell-Hass supplemented with succinate 30 mM. Panels (B) and (D) are respectively TEM and epifluorescence images of cells growing with DBT as the only carbon source. For epifluorescence microscopy Cobetia sp. strain MM1IDA2H-1 cells were stained with BODIPY 505/515 and SYTO 9 to detect respectively lipidic structures and (nucleic acid) cells (red bar = 10 μm).

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Microscopy, Produced, Transmission Electron Microscopy, Epifluorescence Microscopy, Staining

    A. Expression of selected genes of virulence factors of A. salmonicida exposed to biosurfactant. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures (grown to an optical density at 600 nm = 1.0) of A. salmonicida exposed to 80 mg l −1 of biosurfactant (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida not exposed to biosurfactant (NE). In both, the experimental and control conditions, data were normalized to specific 16S RNA-gene of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.B. Expression of selected genes encoding virulence factors of A. salmonicida exposed to Cobetia sp. strain MM1IDA2H-1. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures of A. salmonicida grown with the Cobetia sp. MM1IDA2H-1 strain (optical density at 600 nm = 1.0) (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida (NE). Data from experimental and control conditions were normalized to specific 16S RNA genes of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: A. Expression of selected genes of virulence factors of A. salmonicida exposed to biosurfactant. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures (grown to an optical density at 600 nm = 1.0) of A. salmonicida exposed to 80 mg l −1 of biosurfactant (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida not exposed to biosurfactant (NE). In both, the experimental and control conditions, data were normalized to specific 16S RNA-gene of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.B. Expression of selected genes encoding virulence factors of A. salmonicida exposed to Cobetia sp. strain MM1IDA2H-1. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures of A. salmonicida grown with the Cobetia sp. MM1IDA2H-1 strain (optical density at 600 nm = 1.0) (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida (NE). Data from experimental and control conditions were normalized to specific 16S RNA genes of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Examples of MDA-MB-231 tumors and tumor histology from 5αP- and 3αHP-treated mice . (A) Large aggressive tumor from a 5αP-treated mouse, with histologic sections showing (B) invasion of rib-cage muscle by the spreading tumor cells, and (C) higher magnification of region with numerous mitoses (arrows). (D) Residual tumor from a 3αHP-treated mouse, with no signs of invasion (E) and showing region of tumor with numerous apoptotic and necrotic cells (F) . Formalin-fixed sections (5 μm) stained with hematoxylin and eosin. Scale bars at 1.0 cm for whole tumors (A, D), at 160 µm for (B) and (E), and at 40 µm for (C) and (F).

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Examples of MDA-MB-231 tumors and tumor histology from 5αP- and 3αHP-treated mice . (A) Large aggressive tumor from a 5αP-treated mouse, with histologic sections showing (B) invasion of rib-cage muscle by the spreading tumor cells, and (C) higher magnification of region with numerous mitoses (arrows). (D) Residual tumor from a 3αHP-treated mouse, with no signs of invasion (E) and showing region of tumor with numerous apoptotic and necrotic cells (F) . Formalin-fixed sections (5 μm) stained with hematoxylin and eosin. Scale bars at 1.0 cm for whole tumors (A, D), at 160 µm for (B) and (E), and at 40 µm for (C) and (F).

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Multiple Displacement Amplification, Mouse Assay, Staining

    Summary of the opposing autocrine/paracrine effects of the progesterone metabolites, 5αP and 3αHP, in a stylized ER/PR-negative human breast cell . Evidence presented here shows that a high concentration of 5αP relative to 3αHP, in the microenvironment, promotes initiation and growth of ER/PR-negative human breast cell tumors, whereas a higher concentration of 3αHP, relative to 5αP, suppresses tumorigenesis and promotes normalcy. Progesterone is converted to 3αHP and 5αP in breast cells. Tumorigenic and tumor cells convert more progesterone to 5αP and less to 3αHP than do normal cells. The steroids, being lipophylic, are able to pass out of cells and result in a concentration buildup in the microenvironment. The result is a significant increase in the 5αP-to-3αHP concentration ratio in the microenvironment of tumorigenic cells and within tumorous tissues in comparison with normal (nontumorous) breasts. 3αHP and 5αP bind to specific receptors on the plasma membrane linked to signaling pathways involving PKC, phospholipase C, and Ca 2+ mobilization (3αHP) and MAPK/Erk1/2 (5αP) and to modulators of gene expression. The cancer-inhibiting actions of 3αHP result in decreased proliferation and detachment of cells, increased apoptosis, and suppression of tumor initiation and growth. The cancer-promoting actions of 5αP have the opposite effects and result in stimulation of tumorigenesis and tumor growth. The evidence suggests that high concentrations of 5αP relative to 3αHP in the microenvironment will promote progression toward neoplasia and tumorigenesis, whereas a low 5αP-to-3αHP concentration ratio favors maintenance of the normal state.

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Summary of the opposing autocrine/paracrine effects of the progesterone metabolites, 5αP and 3αHP, in a stylized ER/PR-negative human breast cell . Evidence presented here shows that a high concentration of 5αP relative to 3αHP, in the microenvironment, promotes initiation and growth of ER/PR-negative human breast cell tumors, whereas a higher concentration of 3αHP, relative to 5αP, suppresses tumorigenesis and promotes normalcy. Progesterone is converted to 3αHP and 5αP in breast cells. Tumorigenic and tumor cells convert more progesterone to 5αP and less to 3αHP than do normal cells. The steroids, being lipophylic, are able to pass out of cells and result in a concentration buildup in the microenvironment. The result is a significant increase in the 5αP-to-3αHP concentration ratio in the microenvironment of tumorigenic cells and within tumorous tissues in comparison with normal (nontumorous) breasts. 3αHP and 5αP bind to specific receptors on the plasma membrane linked to signaling pathways involving PKC, phospholipase C, and Ca 2+ mobilization (3αHP) and MAPK/Erk1/2 (5αP) and to modulators of gene expression. The cancer-inhibiting actions of 3αHP result in decreased proliferation and detachment of cells, increased apoptosis, and suppression of tumor initiation and growth. The cancer-promoting actions of 5αP have the opposite effects and result in stimulation of tumorigenesis and tumor growth. The evidence suggests that high concentrations of 5αP relative to 3αHP in the microenvironment will promote progression toward neoplasia and tumorigenesis, whereas a low 5αP-to-3αHP concentration ratio favors maintenance of the normal state.

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Concentration Assay, Expressing

    Hormone levels in tumors . (A) Effect of hormone treatment on 5αP and 3αHP levels in tumors. Hormone levels in tumors from four vehicle-injected, five 5αP-treated, and four 3αHP-treated mice were determined with RIA, as described in Methods. Hormone levels are presented as nanograms per milliliter 5αP and 3αHP and (B) as the ratio of 5αP to 3αHP. (** P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Hormone levels in tumors . (A) Effect of hormone treatment on 5αP and 3αHP levels in tumors. Hormone levels in tumors from four vehicle-injected, five 5αP-treated, and four 3αHP-treated mice were determined with RIA, as described in Methods. Hormone levels are presented as nanograms per milliliter 5αP and 3αHP and (B) as the ratio of 5αP to 3αHP. (** P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Injection, Mouse Assay

    Opposing in vitro effects of 5αP and 3αHP on proliferation of MDA-MB-231 cells used in the in vivo (xenograft) studies . Cells were seeded at 4 × 10 4 cells per dish, allowed to attach for 24 hours, and then treated for 72 hours without (C, control) or with 10 -6 M 5αP and/or 3αHP, and proliferation was determined by cell counts. Data are presented as cell number (mean and SEM; n = 4). ** P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Opposing in vitro effects of 5αP and 3αHP on proliferation of MDA-MB-231 cells used in the in vivo (xenograft) studies . Cells were seeded at 4 × 10 4 cells per dish, allowed to attach for 24 hours, and then treated for 72 hours without (C, control) or with 10 -6 M 5αP and/or 3αHP, and proliferation was determined by cell counts. Data are presented as cell number (mean and SEM; n = 4). ** P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: In Vitro, Multiple Displacement Amplification, In Vivo

    ER/PR-negative breast cell tumor induction and growth are regulated by 5αP and 3αHP . (A) Tumor induction and growth are stimulated by 5αP. MDA-MB-231 cells were implanted in mammary fat pads of 11 mice (day 0, inset); 3 days before (day -3), five mice were injected with vehicle (control; black open circles), and six were injected with 5αP (red, solid circles). Data points represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), and the experiment was terminated on day 40. *Significantly different from controls at P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: ER/PR-negative breast cell tumor induction and growth are regulated by 5αP and 3αHP . (A) Tumor induction and growth are stimulated by 5αP. MDA-MB-231 cells were implanted in mammary fat pads of 11 mice (day 0, inset); 3 days before (day -3), five mice were injected with vehicle (control; black open circles), and six were injected with 5αP (red, solid circles). Data points represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), and the experiment was terminated on day 40. *Significantly different from controls at P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Multiple Displacement Amplification, Mouse Assay, Injection

    3αHP results in suppression and regression of 5αP-induced ER/PR-negative tumors . (A) 3αHP suppresses ER/PR-negative breast cell tumorigenesis in 5αP-pretreated mice. Fourteen mice were treated with 5αP on day -3 and day 11 and then were divided into two groups. One group (Group I) continued to be treated with 5αP, whereas the other group (Group II) was treated with 3αHP on days 27, 36, and 47 (Inset). One mouse from each group was excluded from the final analysis, as explained under Results. The data are presented as the percentage of mice with tumors at termination. (B) 3αHP results in regression of 5αP-induced ER/PR-negative breast cell tumors. Twenty-four mice with MDA-MB-231 cell implants received injections of 5αP on days 0, 20, and 61 (inset); on day 75, the 14 mice with approximately similar-sized small palpable tumors (18 to 34 mm 3 ) were divided into two groups, consisting of seven mice each, which received a single injection of either vehicle (veh) or 3αHP, and the experiment was terminated 24 days later. Bars represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), at the start of treatments (day 75, Initial) and at termination (day 99, Final).

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: 3αHP results in suppression and regression of 5αP-induced ER/PR-negative tumors . (A) 3αHP suppresses ER/PR-negative breast cell tumorigenesis in 5αP-pretreated mice. Fourteen mice were treated with 5αP on day -3 and day 11 and then were divided into two groups. One group (Group I) continued to be treated with 5αP, whereas the other group (Group II) was treated with 3αHP on days 27, 36, and 47 (Inset). One mouse from each group was excluded from the final analysis, as explained under Results. The data are presented as the percentage of mice with tumors at termination. (B) 3αHP results in regression of 5αP-induced ER/PR-negative breast cell tumors. Twenty-four mice with MDA-MB-231 cell implants received injections of 5αP on days 0, 20, and 61 (inset); on day 75, the 14 mice with approximately similar-sized small palpable tumors (18 to 34 mm 3 ) were divided into two groups, consisting of seven mice each, which received a single injection of either vehicle (veh) or 3αHP, and the experiment was terminated 24 days later. Bars represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), at the start of treatments (day 75, Initial) and at termination (day 99, Final).

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Mouse Assay, Multiple Displacement Amplification, Injection

    Hormone levels in serum . (A) Serum hormone levels after treatment. Serum samples ( n = 4 to 5) were analyzed 15 to 22 days and 42 days after mice received an injection of vehicle (control), 5αP, or 3αHP. Data points represent nanograms per milliliter (mean ± SEM). (** P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Hormone levels in serum . (A) Serum hormone levels after treatment. Serum samples ( n = 4 to 5) were analyzed 15 to 22 days and 42 days after mice received an injection of vehicle (control), 5αP, or 3αHP. Data points represent nanograms per milliliter (mean ± SEM). (** P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Mouse Assay, Injection

    Conversion of progesterone to 3α-dihydroprogesterone (3αHP) and 5α-dihydroprogesterone (5αP) . In vitro studies have shown that both ER/PR-positive and -negative human breast tissues and cell lines are able to convert progesterone to 3αHP and 5αP by the actions of 3α-hydroxysteroid oxidoreductase (3α-HSO) and 5α-reductase, respectively.

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Conversion of progesterone to 3α-dihydroprogesterone (3αHP) and 5α-dihydroprogesterone (5αP) . In vitro studies have shown that both ER/PR-positive and -negative human breast tissues and cell lines are able to convert progesterone to 3αHP and 5αP by the actions of 3α-hydroxysteroid oxidoreductase (3α-HSO) and 5α-reductase, respectively.

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: In Vitro

    2D confocal imaging: a qualitative analysis. Confocal image stacks of 72-h S. mutans UA 159 biofilms following topical treatment with (a) A. gratissima : fraction Ag 4 ; (b) B. dracunculifolia : fraction Bd 2 ; (c) C. sativum : fraction Cs 4 ; (d) L. sidoides : fraction Ls 3 ; and (e) vehicle (propylene glycol, 6.25% v/v). The structures depicted in red (Dextran, Alexa Fluor 6) represent the extracellular polysaccharides that constitute the biofilm matrix, while the structures depicted in green (Syto 9) are metabolically active bacterial cells (optical magnitude 63x). It can be noted that all bioactive fractions ((a)–(d)) affected the EPS matrix making it less intimately interspersed between and over the cells than did the vehicle alone (e).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis

    doi: 10.1155/2015/871316

    Figure Lengend Snippet: 2D confocal imaging: a qualitative analysis. Confocal image stacks of 72-h S. mutans UA 159 biofilms following topical treatment with (a) A. gratissima : fraction Ag 4 ; (b) B. dracunculifolia : fraction Bd 2 ; (c) C. sativum : fraction Cs 4 ; (d) L. sidoides : fraction Ls 3 ; and (e) vehicle (propylene glycol, 6.25% v/v). The structures depicted in red (Dextran, Alexa Fluor 6) represent the extracellular polysaccharides that constitute the biofilm matrix, while the structures depicted in green (Syto 9) are metabolically active bacterial cells (optical magnitude 63x). It can be noted that all bioactive fractions ((a)–(d)) affected the EPS matrix making it less intimately interspersed between and over the cells than did the vehicle alone (e).

    Article Snippet: Phytochemical Analysis of the Essential Oils and Bioactive Fractions by Gas Chromatography Coupled to Mass Spectrometry (GC-MS) Volatile constituents were identified using a Hewlett-Packard 6890 gas chromatograph coupled with an HP-5975 mass selective detector and HP-5 capillary column (30 m × 0.25 mm × 0.25 μ m diameter).

    Techniques: Imaging, Metabolic Labelling

    Synergistic mechanisms of anticancer activities induced by MC ‐4 and everolimus alone and in combination. A, Effect of MC ‐4 on the anticancer signaling pathways in RCC cells. Caki‐1 and 786‐O cells were treated with MC ‐4 for 24 h, and then Western blotting was performed. Representative blots are shown. B, Representative photomicrographs of immunohistochemical ( IHC ) analysis of PKM 2 (bottom) in the indicated tumors from mice treated with MC ‐4 or vehicle (lower panel). C, Combination effects of MC ‐4 and everolimus on cell viability. RCC cell lines (Caki‐1 and 786‐O) were treated for 72 h with MC ‐4 in combination with everolimus. Cell viability was determined by MTT assay and is expressed relative to the value without treatment. The data are means ± SEM from three independent experiments. ** P

    Journal: Cancer Medicine

    Article Title: Novel therapeutic roles of MC‐4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways, et al. Novel therapeutic roles of MC‐4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways

    doi: 10.1002/cam4.1748

    Figure Lengend Snippet: Synergistic mechanisms of anticancer activities induced by MC ‐4 and everolimus alone and in combination. A, Effect of MC ‐4 on the anticancer signaling pathways in RCC cells. Caki‐1 and 786‐O cells were treated with MC ‐4 for 24 h, and then Western blotting was performed. Representative blots are shown. B, Representative photomicrographs of immunohistochemical ( IHC ) analysis of PKM 2 (bottom) in the indicated tumors from mice treated with MC ‐4 or vehicle (lower panel). C, Combination effects of MC ‐4 and everolimus on cell viability. RCC cell lines (Caki‐1 and 786‐O) were treated for 72 h with MC ‐4 in combination with everolimus. Cell viability was determined by MTT assay and is expressed relative to the value without treatment. The data are means ± SEM from three independent experiments. ** P

    Article Snippet: The subfraction MC‐4, which showed the highest cytotoxicity against Caki‐1 and 786‐O RCC cell lines, was characterized by gas chromatography–mass spectrometry (GC/MS) analysis (Hewlett Packard HP 6890 series GC system with a Hewlett Packard 5973 mass selective detector) (Figure A,B).

    Techniques: Western Blot, Immunohistochemistry, Mouse Assay, MTT Assay

    Cytotoxicity of each component isolated from MC ‐4 against human RCC cell lines. A, Gas chromatography ( GC ) chromatogram of MC ‐4 and analytical conditions for GC / MS . B, The structures of some of the compounds identified in MC ‐4 extract. C, Caki‐1 and 786‐O cells were seeded on 96‐well plates at a final concentration of 2 × 10 3 cells per 100 μL medium per well at 37°C to allow cell attachment. After 24‐h incubation, the cells were treated with 6,10,14‐trimethyl pentadecan‐2‐one, ethyl linoleate, ethyl linolenate, and ethyl palmitate up to 80 μg/mL for 48 h. Cytotoxicity was determined using MTT assay and the results are expressed as percentages of cytotoxicity relative to the untreated control. Data are expressed as mean ± SEM. * P

    Journal: Cancer Medicine

    Article Title: Novel therapeutic roles of MC‐4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways, et al. Novel therapeutic roles of MC‐4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways

    doi: 10.1002/cam4.1748

    Figure Lengend Snippet: Cytotoxicity of each component isolated from MC ‐4 against human RCC cell lines. A, Gas chromatography ( GC ) chromatogram of MC ‐4 and analytical conditions for GC / MS . B, The structures of some of the compounds identified in MC ‐4 extract. C, Caki‐1 and 786‐O cells were seeded on 96‐well plates at a final concentration of 2 × 10 3 cells per 100 μL medium per well at 37°C to allow cell attachment. After 24‐h incubation, the cells were treated with 6,10,14‐trimethyl pentadecan‐2‐one, ethyl linoleate, ethyl linolenate, and ethyl palmitate up to 80 μg/mL for 48 h. Cytotoxicity was determined using MTT assay and the results are expressed as percentages of cytotoxicity relative to the untreated control. Data are expressed as mean ± SEM. * P

    Article Snippet: The subfraction MC‐4, which showed the highest cytotoxicity against Caki‐1 and 786‐O RCC cell lines, was characterized by gas chromatography–mass spectrometry (GC/MS) analysis (Hewlett Packard HP 6890 series GC system with a Hewlett Packard 5973 mass selective detector) (Figure A,B).

    Techniques: Isolation, Gas Chromatography, Gas Chromatography-Mass Spectrometry, Concentration Assay, Cell Attachment Assay, Incubation, MTT Assay

    Anticancer effects of MC ‐4 in human RCC cells. A, Effects of MC ‐4 on the cell cycle. Caki‐1 and 786‐O cells were treated with MC ‐4 (25, 50, or 100 μg/mL) for 24 h. After incubation, the cells were stained with propidium iodide ( PI ) and then analyzed using a flow cytometer (left panel). The expression levels of cell cycle‐regulated proteins were measured by Western blot analysis (right panel). B, Effects of MC ‐4 on apoptosis. Scatter plots present the percentage of viable, early apoptotic, late apoptotic, and necrotic cells in untreated (control) and treated cells (left panel). The cells were treated with MC ‐4 (25, 50, or 100 μg/mL) for 24 h. The expression levels of apoptosis‐related proteins were measured by Western blotting (right panel). C, Effects of MC ‐4 on autophagy. Monodansylcadaverine ( MDC ) staining shows that autophagy was activated in Caki‐1 and 786‐O cells after treatment with MC ‐4. Magnification ×400 (left panels). The expression levels of autophagy‐related proteins in RCC cells were measured after MC ‐4 treatment. Western blot analysis was performed using p62, beclin‐1, LC 3‐I/ II , and ATG 5 antibodies. Equal loading and transfer were verified by reprobing the membranes with β‐actin antibody (right panels). D, Effects of MC ‐4 on tumor growth in nude mice inoculated with Caki‐1 or 786‐O cells. During the 30‐d treatment, tumor volumes were estimated using measurements taken with calipers (mm 3 ). The histogram data shown are average tumor volumes and tumor weights (mean ± SEM, n = 5). ** P

    Journal: Cancer Medicine

    Article Title: Novel therapeutic roles of MC‐4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways, et al. Novel therapeutic roles of MC‐4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways

    doi: 10.1002/cam4.1748

    Figure Lengend Snippet: Anticancer effects of MC ‐4 in human RCC cells. A, Effects of MC ‐4 on the cell cycle. Caki‐1 and 786‐O cells were treated with MC ‐4 (25, 50, or 100 μg/mL) for 24 h. After incubation, the cells were stained with propidium iodide ( PI ) and then analyzed using a flow cytometer (left panel). The expression levels of cell cycle‐regulated proteins were measured by Western blot analysis (right panel). B, Effects of MC ‐4 on apoptosis. Scatter plots present the percentage of viable, early apoptotic, late apoptotic, and necrotic cells in untreated (control) and treated cells (left panel). The cells were treated with MC ‐4 (25, 50, or 100 μg/mL) for 24 h. The expression levels of apoptosis‐related proteins were measured by Western blotting (right panel). C, Effects of MC ‐4 on autophagy. Monodansylcadaverine ( MDC ) staining shows that autophagy was activated in Caki‐1 and 786‐O cells after treatment with MC ‐4. Magnification ×400 (left panels). The expression levels of autophagy‐related proteins in RCC cells were measured after MC ‐4 treatment. Western blot analysis was performed using p62, beclin‐1, LC 3‐I/ II , and ATG 5 antibodies. Equal loading and transfer were verified by reprobing the membranes with β‐actin antibody (right panels). D, Effects of MC ‐4 on tumor growth in nude mice inoculated with Caki‐1 or 786‐O cells. During the 30‐d treatment, tumor volumes were estimated using measurements taken with calipers (mm 3 ). The histogram data shown are average tumor volumes and tumor weights (mean ± SEM, n = 5). ** P

    Article Snippet: The subfraction MC‐4, which showed the highest cytotoxicity against Caki‐1 and 786‐O RCC cell lines, was characterized by gas chromatography–mass spectrometry (GC/MS) analysis (Hewlett Packard HP 6890 series GC system with a Hewlett Packard 5973 mass selective detector) (Figure A,B).

    Techniques: Incubation, Staining, Flow Cytometry, Cytometry, Expressing, Western Blot, Mouse Assay