Structured Review

Agilent technologies i pachyphyllum fruits
Constituent compounds isolated from the essential oil of Illicium <t>pachyphyllum</t> fruits.
I Pachyphyllum Fruits, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Chemical Composition and Insecticidal Activity of the Essential Oil of Illicium pachyphyllum Fruits against Two Grain Storage Insects"

Article Title: Chemical Composition and Insecticidal Activity of the Essential Oil of Illicium pachyphyllum Fruits against Two Grain Storage Insects

Journal: Molecules

doi: 10.3390/molecules171214870

Constituent compounds isolated from the essential oil of Illicium pachyphyllum fruits.
Figure Legend Snippet: Constituent compounds isolated from the essential oil of Illicium pachyphyllum fruits.

Techniques Used: Isolation

2) Product Images from "(3S,6E)-Nerolidol-mediated rendezvous of Cyclocephalini beetles, Cyclocephala paraguayensis, in bottle gourd flowers"

Article Title: (3S,6E)-Nerolidol-mediated rendezvous of Cyclocephalini beetles, Cyclocephala paraguayensis, in bottle gourd flowers

Journal: bioRxiv

doi: 10.1101/2020.06.09.142059

Analysis of bottle gourd flowers, Lagenaria siceraria , extract, and synthetic isomers of nerolidol by gas chromatography equipped with a chiral column. a) Z and E -nerolidol (left to right) isomers of nerolidol (a mix of isomers); b) Z -nerolidol isomers; c) Synthetic ( 3R,6E )-nerolidol (gift from Dr. Robert Hanus, Czech Republic); d) ( E )-nerolidol from Sigma-Aldrich; e) Mix of 40 ng from each ( 3R,6E )-nerolidol and ( 3S,6E )-nerolidol; f) Natural extract from VOCs of L. siceraria flowers; g) Coinjection of “e” and “f” chromatogram extracts.
Figure Legend Snippet: Analysis of bottle gourd flowers, Lagenaria siceraria , extract, and synthetic isomers of nerolidol by gas chromatography equipped with a chiral column. a) Z and E -nerolidol (left to right) isomers of nerolidol (a mix of isomers); b) Z -nerolidol isomers; c) Synthetic ( 3R,6E )-nerolidol (gift from Dr. Robert Hanus, Czech Republic); d) ( E )-nerolidol from Sigma-Aldrich; e) Mix of 40 ng from each ( 3R,6E )-nerolidol and ( 3S,6E )-nerolidol; f) Natural extract from VOCs of L. siceraria flowers; g) Coinjection of “e” and “f” chromatogram extracts.

Techniques Used: Gas Chromatography

Mean number of Cyclocephalini beetle, Cyclocephala paraguayensis , caught per replicate in field experiments conducted in Cassilândia, MS, Brazil. Treatments: Nerolidol (a mix of isomers) = mixture of four isomers of nerolidol; (3 S , 6 E )-nerolidol = synthetic nerolidol, which is identical to the stereoisomer of nerolidol produced by L. siceraria flowers. A) Mean number of adults of C. paraguayensis caught per replicate of nerolidol-baited traps and controls. *Treatment is significantly different at P
Figure Legend Snippet: Mean number of Cyclocephalini beetle, Cyclocephala paraguayensis , caught per replicate in field experiments conducted in Cassilândia, MS, Brazil. Treatments: Nerolidol (a mix of isomers) = mixture of four isomers of nerolidol; (3 S , 6 E )-nerolidol = synthetic nerolidol, which is identical to the stereoisomer of nerolidol produced by L. siceraria flowers. A) Mean number of adults of C. paraguayensis caught per replicate of nerolidol-baited traps and controls. *Treatment is significantly different at P

Techniques Used: Produced

3) Product Images from "Identification of Larvicidal Constituents of the Essential Oil of Echinops grijsii Roots against the Three Species of Mosquitoes"

Article Title: Identification of Larvicidal Constituents of the Essential Oil of Echinops grijsii Roots against the Three Species of Mosquitoes

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules22020205

Larvicidal thiophenes isolated from the essential oil of Echinops grijsii roots.
Figure Legend Snippet: Larvicidal thiophenes isolated from the essential oil of Echinops grijsii roots.

Techniques Used: Isolation

4) Product Images from "Identification of Repellent and Insecticidal Constituents of the Essential Oil of Artemisia rupestris L. Aerial Parts against Liposcelis bostrychophila Badonnel"

Article Title: Identification of Repellent and Insecticidal Constituents of the Essential Oil of Artemisia rupestris L. Aerial Parts against Liposcelis bostrychophila Badonnel

Journal: Molecules

doi: 10.3390/molecules180910733

Constituent compounds isolated from the essential oil of Artemisia rupestris aerial parts.
Figure Legend Snippet: Constituent compounds isolated from the essential oil of Artemisia rupestris aerial parts.

Techniques Used: Isolation

5) Product Images from "Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development"

Article Title: Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development

Journal: Molecular Nutrition & Food Research

doi: 10.1002/mnfr.201700942

MOS increased fecal bile acid excretion. A) The fecal primary BAs cholic acid (CA), a‐muricholic acid (a‐MCA), β‐muricholic acid (β‐MCA), B) the fecal secondary hyocholic acid (HCA), deoxycholic acid (DCA), ω‐muricholic acid (ω‐MCA), C) plasma total BAs, and D) mRNA expression analysis of 7‐α‐hydroxylase ( Cyp7a1 ) and sterol 27‐hydroxylase ( Cyp27a1 ) were determined in mice fed a WTD with or without MOS for 14 weeks. Open bars/circles represent the control group and closed bars/circles represent the MOS group. Values are presented as means ± SEM ( n = 8–15 mice per group). * p
Figure Legend Snippet: MOS increased fecal bile acid excretion. A) The fecal primary BAs cholic acid (CA), a‐muricholic acid (a‐MCA), β‐muricholic acid (β‐MCA), B) the fecal secondary hyocholic acid (HCA), deoxycholic acid (DCA), ω‐muricholic acid (ω‐MCA), C) plasma total BAs, and D) mRNA expression analysis of 7‐α‐hydroxylase ( Cyp7a1 ) and sterol 27‐hydroxylase ( Cyp27a1 ) were determined in mice fed a WTD with or without MOS for 14 weeks. Open bars/circles represent the control group and closed bars/circles represent the MOS group. Values are presented as means ± SEM ( n = 8–15 mice per group). * p

Techniques Used: High Content Screening, Expressing, Mouse Assay

6) Product Images from "Chemical Profile of the Organic Residue from Ancient Amphora Found in the Adriatic Sea Determined by Direct GC and GC-MS Analysis"

Article Title: Chemical Profile of the Organic Residue from Ancient Amphora Found in the Adriatic Sea Determined by Direct GC and GC-MS Analysis

Journal: Molecules

doi: 10.3390/molecules16097936

Total ion chromatogram of the organic residue solution in CH 2 Cl 2 on HP-5MS column. Numbers refer to Table 1 .
Figure Legend Snippet: Total ion chromatogram of the organic residue solution in CH 2 Cl 2 on HP-5MS column. Numbers refer to Table 1 .

Techniques Used:

Total ion chromatogram of the organic residue headspace composition obtained by HS-SPME (PDMS/DVB fiber) on HP-5MS column. Numbers refer to Table 1 .
Figure Legend Snippet: Total ion chromatogram of the organic residue headspace composition obtained by HS-SPME (PDMS/DVB fiber) on HP-5MS column. Numbers refer to Table 1 .

Techniques Used: Solid-phase Microextraction

( a ) Scheme of the Greco-Italian amphora type Benoit Republicane-II/Lamboglia with the section representing the coating. ( b ) Total ion chromatogram of the organic residue headspace composition obtained by HS-SPME (DVB/CAR/PDMS fibre) on HP-5MS column. Numbers refer to Table 1 .
Figure Legend Snippet: ( a ) Scheme of the Greco-Italian amphora type Benoit Republicane-II/Lamboglia with the section representing the coating. ( b ) Total ion chromatogram of the organic residue headspace composition obtained by HS-SPME (DVB/CAR/PDMS fibre) on HP-5MS column. Numbers refer to Table 1 .

Techniques Used: Solid-phase Microextraction

7) Product Images from "Characterization of Two Historic Smallpox Specimens from a Czech Museum"

Article Title: Characterization of Two Historic Smallpox Specimens from a Czech Museum

Journal: Viruses

doi: 10.3390/v9080200

Maximum credibility tree for variola virus genomes, split into the major clades P-I and P-II. The chronogram was generated using BEAST software and is based on the highly conserved central genome region (104,142 bp). The posterior probabilities of all clades are > 95% unless stated otherwise close to the nodes. Sequences of specimens V563 and V1588 are shown in red.
Figure Legend Snippet: Maximum credibility tree for variola virus genomes, split into the major clades P-I and P-II. The chronogram was generated using BEAST software and is based on the highly conserved central genome region (104,142 bp). The posterior probabilities of all clades are > 95% unless stated otherwise close to the nodes. Sequences of specimens V563 and V1588 are shown in red.

Techniques Used: Generated, Software

Anatomical specimens V1588 and V563 labeled “Variola”, Czech National Museum, Prague.
Figure Legend Snippet: Anatomical specimens V1588 and V563 labeled “Variola”, Czech National Museum, Prague.

Techniques Used: Labeling

Mass spectrometric (MS) proteomic analysis. ( A ) Representative MS/MS spectrum of variola virus-specific peptide (NDDVLFR) from sample V1588. The spectrum depicts the intensity ( y -axis) of analyzed mass-to-charge ratios of identified peptide fragments ( x -axis). Based on this spectrum, fragments y4, y5 and y6 were involved in the Selected Reaction Monitoring (SRM) technique. ( B ) Selected Reaction Monitoring chromatogram of peptide (NDDVLFR) of sample V563. The peptide digest (in red) was analyzed with spiked synthetic, heavy-labeled counterpart (in blue). The presence of both peaks at a retention time of 14.4 min (based on prior heavy peptide analysis) together with the consistency of intensities of the ion pairs is an unambiguous evidence of the presence of peptide (NDDVLFR).
Figure Legend Snippet: Mass spectrometric (MS) proteomic analysis. ( A ) Representative MS/MS spectrum of variola virus-specific peptide (NDDVLFR) from sample V1588. The spectrum depicts the intensity ( y -axis) of analyzed mass-to-charge ratios of identified peptide fragments ( x -axis). Based on this spectrum, fragments y4, y5 and y6 were involved in the Selected Reaction Monitoring (SRM) technique. ( B ) Selected Reaction Monitoring chromatogram of peptide (NDDVLFR) of sample V563. The peptide digest (in red) was analyzed with spiked synthetic, heavy-labeled counterpart (in blue). The presence of both peaks at a retention time of 14.4 min (based on prior heavy peptide analysis) together with the consistency of intensities of the ion pairs is an unambiguous evidence of the presence of peptide (NDDVLFR).

Techniques Used: Mass Spectrometry, Labeling

8) Product Images from "Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development"

Article Title: Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma Cholesterol and Atherosclerosis Development

Journal: Molecular Nutrition & Food Research

doi: 10.1002/mnfr.201700942

MOS increased fecal bile acid excretion. A) The fecal primary BAs cholic acid (CA), a‐muricholic acid (a‐MCA), β‐muricholic acid (β‐MCA), B) the fecal secondary hyocholic acid (HCA), deoxycholic acid (DCA), ω‐muricholic acid (ω‐MCA), C) plasma total BAs, and D) mRNA expression analysis of 7‐α‐hydroxylase ( Cyp7a1 ) and sterol 27‐hydroxylase ( Cyp27a1 ) were determined in mice fed a WTD with or without MOS for 14 weeks. Open bars/circles represent the control group and closed bars/circles represent the MOS group. Values are presented as means ± SEM ( n = 8–15 mice per group). * p
Figure Legend Snippet: MOS increased fecal bile acid excretion. A) The fecal primary BAs cholic acid (CA), a‐muricholic acid (a‐MCA), β‐muricholic acid (β‐MCA), B) the fecal secondary hyocholic acid (HCA), deoxycholic acid (DCA), ω‐muricholic acid (ω‐MCA), C) plasma total BAs, and D) mRNA expression analysis of 7‐α‐hydroxylase ( Cyp7a1 ) and sterol 27‐hydroxylase ( Cyp27a1 ) were determined in mice fed a WTD with or without MOS for 14 weeks. Open bars/circles represent the control group and closed bars/circles represent the MOS group. Values are presented as means ± SEM ( n = 8–15 mice per group). * p

Techniques Used: High Content Screening, Expressing, Mouse Assay

9) Product Images from "Preparation of honokiol nanoparticles by liquid antisolvent precipitation technique, characterization, pharmacokinetics, and evaluation of inhibitory effect on HepG2 cells"

Article Title: Preparation of honokiol nanoparticles by liquid antisolvent precipitation technique, characterization, pharmacokinetics, and evaluation of inhibitory effect on HepG2 cells

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S178416

Gas chromatography analysis results. Notes: ( A ) The gas phase diagram of 0.05 mg/mL ethanol standard solution; ( B ) the gas phase diagram of 10 mg/mL ethanol–water solution in the honokiol nanoparticles.
Figure Legend Snippet: Gas chromatography analysis results. Notes: ( A ) The gas phase diagram of 0.05 mg/mL ethanol standard solution; ( B ) the gas phase diagram of 10 mg/mL ethanol–water solution in the honokiol nanoparticles.

Techniques Used: Gas Chromatography

10) Product Images from "A bacterial chloroform reductive dehalogenase: purification and biochemical characterization"

Article Title: A bacterial chloroform reductive dehalogenase: purification and biochemical characterization

Journal: Microbial Biotechnology

doi: 10.1111/1751-7915.12745

Deduced amino acid sequence of TmrA from strain UNSWDHB . The black and grey boxes indicate the TAT signal peptide and a [4Fe‐4S] double cluster binding domain ( NCBI : pfam13484), respectively. Underlined sequences are peptides that were detected by mass spectrometry with MASCOT (coverage 77.3%, score of 2223). A slash indicates the predicted location of the cleavage of the peptide signal predicted by PRED ‐ TAT .
Figure Legend Snippet: Deduced amino acid sequence of TmrA from strain UNSWDHB . The black and grey boxes indicate the TAT signal peptide and a [4Fe‐4S] double cluster binding domain ( NCBI : pfam13484), respectively. Underlined sequences are peptides that were detected by mass spectrometry with MASCOT (coverage 77.3%, score of 2223). A slash indicates the predicted location of the cleavage of the peptide signal predicted by PRED ‐ TAT .

Techniques Used: Sequencing, Binding Assay, Mass Spectrometry

The purified TmrA. A. SDS ‐ PAGE with the purified chloroform reductive dehalogenase, TmrA, of Dehalobacter UNSWDHB in lane 4 . Molecular size markers (SeeBlue Plus2 Prestained Standard; Thermo Fisher Scientific) are shown in lane 1 and 5 . The crude cell lysate and solubilized membrane fraction are in lane 2 and 3 . The arrow indicates the purified protein band. The gel was stained with GelCode Blue Stain Reagent (Thermo Fisher Scientific). B. MALDI ‐ TOF spectra of the TmrA at an intact mass of 44 511 Da.
Figure Legend Snippet: The purified TmrA. A. SDS ‐ PAGE with the purified chloroform reductive dehalogenase, TmrA, of Dehalobacter UNSWDHB in lane 4 . Molecular size markers (SeeBlue Plus2 Prestained Standard; Thermo Fisher Scientific) are shown in lane 1 and 5 . The crude cell lysate and solubilized membrane fraction are in lane 2 and 3 . The arrow indicates the purified protein band. The gel was stained with GelCode Blue Stain Reagent (Thermo Fisher Scientific). B. MALDI ‐ TOF spectra of the TmrA at an intact mass of 44 511 Da.

Techniques Used: Purification, SDS Page, Staining

11) Product Images from "Characterization of Two Historic Smallpox Specimens from a Czech Museum"

Article Title: Characterization of Two Historic Smallpox Specimens from a Czech Museum

Journal: Viruses

doi: 10.3390/v9080200

Maximum credibility tree for variola virus genomes, split into the major clades P-I and P-II. The chronogram was generated using BEAST software and is based on the highly conserved central genome region (104,142 bp). The posterior probabilities of all clades are > 95% unless stated otherwise close to the nodes. Sequences of specimens V563 and V1588 are shown in red.
Figure Legend Snippet: Maximum credibility tree for variola virus genomes, split into the major clades P-I and P-II. The chronogram was generated using BEAST software and is based on the highly conserved central genome region (104,142 bp). The posterior probabilities of all clades are > 95% unless stated otherwise close to the nodes. Sequences of specimens V563 and V1588 are shown in red.

Techniques Used: Generated, Software

Anatomical specimens V1588 and V563 labeled “Variola”, Czech National Museum, Prague.
Figure Legend Snippet: Anatomical specimens V1588 and V563 labeled “Variola”, Czech National Museum, Prague.

Techniques Used: Labeling

Electron microscopy of specimen V563 showing large numbers of typical orthopoxvirus particles at different magnification. Scale bars correspond to 2 micrometers ( left ) and 200 nm ( right ).
Figure Legend Snippet: Electron microscopy of specimen V563 showing large numbers of typical orthopoxvirus particles at different magnification. Scale bars correspond to 2 micrometers ( left ) and 200 nm ( right ).

Techniques Used: Electron Microscopy

Mass spectrometric (MS) proteomic analysis. ( A ) Representative MS/MS spectrum of variola virus-specific peptide (NDDVLFR) from sample V1588. The spectrum depicts the intensity ( y -axis) of analyzed mass-to-charge ratios of identified peptide fragments ( x -axis). Based on this spectrum, fragments y4, y5 and y6 were involved in the Selected Reaction Monitoring (SRM) technique. ( B ) Selected Reaction Monitoring chromatogram of peptide (NDDVLFR) of sample V563. The peptide digest (in red) was analyzed with spiked synthetic, heavy-labeled counterpart (in blue). The presence of both peaks at a retention time of 14.4 min (based on prior heavy peptide analysis) together with the consistency of intensities of the ion pairs is an unambiguous evidence of the presence of peptide (NDDVLFR).
Figure Legend Snippet: Mass spectrometric (MS) proteomic analysis. ( A ) Representative MS/MS spectrum of variola virus-specific peptide (NDDVLFR) from sample V1588. The spectrum depicts the intensity ( y -axis) of analyzed mass-to-charge ratios of identified peptide fragments ( x -axis). Based on this spectrum, fragments y4, y5 and y6 were involved in the Selected Reaction Monitoring (SRM) technique. ( B ) Selected Reaction Monitoring chromatogram of peptide (NDDVLFR) of sample V563. The peptide digest (in red) was analyzed with spiked synthetic, heavy-labeled counterpart (in blue). The presence of both peaks at a retention time of 14.4 min (based on prior heavy peptide analysis) together with the consistency of intensities of the ion pairs is an unambiguous evidence of the presence of peptide (NDDVLFR).

Techniques Used: Mass Spectrometry, Labeling

Agarose gel electrophoresis of ethidium bromide-stained DNA extracted from specimen V563.
Figure Legend Snippet: Agarose gel electrophoresis of ethidium bromide-stained DNA extracted from specimen V563.

Techniques Used: Agarose Gel Electrophoresis, Staining

12) Product Images from "Cytokines Driven Anti-Inflammatory and Anti-Psoriasis Like Efficacies of Nutraceutical Sea Buckthorn (Hippophae rhamnoides) Oil"

Article Title: Cytokines Driven Anti-Inflammatory and Anti-Psoriasis Like Efficacies of Nutraceutical Sea Buckthorn (Hippophae rhamnoides) Oil

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2019.01186

Gas chromatography–flame ionized detector (GC–FID) chromatogram of sea buckthorn oil (SBKT). (A) The fatty acid composition of the SBKT was determined using the GC–FID methodology. Individual fatty acids were identified and quantified using fatty acid methyl ester methodology. Chromatography analysis identified 16 major fatty acids. (B) Pie-diagram represents the percentage of three categories of fatty acids identified in the SBKT—saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) (also see Table 1 ).
Figure Legend Snippet: Gas chromatography–flame ionized detector (GC–FID) chromatogram of sea buckthorn oil (SBKT). (A) The fatty acid composition of the SBKT was determined using the GC–FID methodology. Individual fatty acids were identified and quantified using fatty acid methyl ester methodology. Chromatography analysis identified 16 major fatty acids. (B) Pie-diagram represents the percentage of three categories of fatty acids identified in the SBKT—saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) (also see Table 1 ).

Techniques Used: Gas Chromatography, Chromatography

13) Product Images from "Oxidation and Polymerization of Triacylglycerols: In-Depth Investigations towards the Impact of Heating Profiles"

Article Title: Oxidation and Polymerization of Triacylglycerols: In-Depth Investigations towards the Impact of Heating Profiles

Journal: Foods

doi: 10.3390/foods8100475

( a ) Sample gas chromatograph (GC) chromatogram for the determination of epoxy, keto, and hydroxy acids and ( b ) sample high performance size exclusion chromatography (HPSEC) chromatogram for the determination of polar fraction distribution (TAGO: Triacylglycerol oligomer; TAGD: Triacylglycerol dimer; oxTAG: Monomeric oxidized triacylglycerol; DAG: Diacylglycerol; and FFA: Free fatty acids).
Figure Legend Snippet: ( a ) Sample gas chromatograph (GC) chromatogram for the determination of epoxy, keto, and hydroxy acids and ( b ) sample high performance size exclusion chromatography (HPSEC) chromatogram for the determination of polar fraction distribution (TAGO: Triacylglycerol oligomer; TAGD: Triacylglycerol dimer; oxTAG: Monomeric oxidized triacylglycerol; DAG: Diacylglycerol; and FFA: Free fatty acids).

Techniques Used: Size-exclusion Chromatography

14) Product Images from "Combinatorial engineering of hybrid mevalonate pathways in Escherichiacoli for protoilludene production"

Article Title: Combinatorial engineering of hybrid mevalonate pathways in Escherichiacoli for protoilludene production

Journal: Microbial Cell Factories

doi: 10.1186/s12934-016-0409-7

Establishment of a protoilludene biosynthesis pathway in E . coli a Expression construct of AO portion. AO operon, which is composed of FPP synthase ispA from E . coli and protoilludene synthase OMP7 from O . olearius , is cloned into pTrc99A vector and designated as pTAO. Ec, the gene from E. coli ; Oo, the gene from O. olearius . b Identification of protoilludene product. The decane phase from two-phase culture of E. coli AO/NA strain was subjected to GC and GC–MS analysis. c Protoilludene production and mevalonate accumulation in culture of the strain E. coli AO/NA. The strain was grown at 30 °C in 4 mL of 2YT medium with 2 % (v/v) glycerol for 24 and 48 h, and overlaid with 1 mL of decane. The dark gray and light gray bars indicate protoilludene production and mevalonate accumulation, respectively
Figure Legend Snippet: Establishment of a protoilludene biosynthesis pathway in E . coli a Expression construct of AO portion. AO operon, which is composed of FPP synthase ispA from E . coli and protoilludene synthase OMP7 from O . olearius , is cloned into pTrc99A vector and designated as pTAO. Ec, the gene from E. coli ; Oo, the gene from O. olearius . b Identification of protoilludene product. The decane phase from two-phase culture of E. coli AO/NA strain was subjected to GC and GC–MS analysis. c Protoilludene production and mevalonate accumulation in culture of the strain E. coli AO/NA. The strain was grown at 30 °C in 4 mL of 2YT medium with 2 % (v/v) glycerol for 24 and 48 h, and overlaid with 1 mL of decane. The dark gray and light gray bars indicate protoilludene production and mevalonate accumulation, respectively

Techniques Used: Expressing, Construct, Clone Assay, Plasmid Preparation, Gas Chromatography-Mass Spectrometry

Coordination of the lower (MvL) and upper (MvU) portions of MVA pathway for protoilludene production. a Expression constructs of the MvU portions with alternations of promoter and copy-number. MvU operon consists of MvaE and MvaS from E. faecalis . The two genes are cloned into pBBR1MCS-2, pSTV28 and pTrc99A vectors, which are designated as pBMvU L , pSMvU M and pTMvU H , respectively. Ef, the gene from E. faecalis ; Plac and Ptrc, lac promoter and trc promoter, respectively. Rectangles show replication origin of each plasmid. b Mevalonate production capacity of recombinant E . coli harboring each of pBMvU L , pSMvU M and pTMvU H . The strains were cultured in 2YT medium at 30 °C for 48 h. c and d Effect of combinations of MvU L,M,H and MvL 1–6 on protoilludene production and mevalonate accumulation. The culture were carried out in 4 mL of 2YT medium containing 2 % (v/v) glycerol with overlay of 1 mL decane at 30 °C for 48 h
Figure Legend Snippet: Coordination of the lower (MvL) and upper (MvU) portions of MVA pathway for protoilludene production. a Expression constructs of the MvU portions with alternations of promoter and copy-number. MvU operon consists of MvaE and MvaS from E. faecalis . The two genes are cloned into pBBR1MCS-2, pSTV28 and pTrc99A vectors, which are designated as pBMvU L , pSMvU M and pTMvU H , respectively. Ef, the gene from E. faecalis ; Plac and Ptrc, lac promoter and trc promoter, respectively. Rectangles show replication origin of each plasmid. b Mevalonate production capacity of recombinant E . coli harboring each of pBMvU L , pSMvU M and pTMvU H . The strains were cultured in 2YT medium at 30 °C for 48 h. c and d Effect of combinations of MvU L,M,H and MvL 1–6 on protoilludene production and mevalonate accumulation. The culture were carried out in 4 mL of 2YT medium containing 2 % (v/v) glycerol with overlay of 1 mL decane at 30 °C for 48 h

Techniques Used: Expressing, Construct, Clone Assay, Plasmid Preparation, Recombinant, Cell Culture

Optimization of the lower (MvL) portion of MVA pathway by sequential order permutation. a Expression constructs of the sequential order permutated MvL portions. MvL operon, containing MvaK1 , MvaK2 and MvaD from S. pneumoniae , and IDI from E. coli , is cloned into pSTV28 vector. Sn, the gene from S. pneumoniae ; Ec, the gene from E. coli ; Plac, lac promoter. b Effect of sequential order permutated MvLs on protoilludene production and cell growth. The strains were grown for 48 h at 30 °C in 4 mL of 2YT medium containing 2 % (v/v) glycerol and 4 mM mevalonate with overlay of 1 mL decane. The dark and light gray bars indicate protoilludene production and cell growth, respectively
Figure Legend Snippet: Optimization of the lower (MvL) portion of MVA pathway by sequential order permutation. a Expression constructs of the sequential order permutated MvL portions. MvL operon, containing MvaK1 , MvaK2 and MvaD from S. pneumoniae , and IDI from E. coli , is cloned into pSTV28 vector. Sn, the gene from S. pneumoniae ; Ec, the gene from E. coli ; Plac, lac promoter. b Effect of sequential order permutated MvLs on protoilludene production and cell growth. The strains were grown for 48 h at 30 °C in 4 mL of 2YT medium containing 2 % (v/v) glycerol and 4 mM mevalonate with overlay of 1 mL decane. The dark and light gray bars indicate protoilludene production and cell growth, respectively

Techniques Used: Expressing, Construct, Clone Assay, Plasmid Preparation

Schematic diagram of protoilludene biosynthesis via mevalonate (MVA) pathway. Protoilludene biosynthesis pathway is divided into three portions: MvU (acetyl-CoA to mevalonate), MvL (mevalonate to IPP and DMAPP) and AO (IPP and DMAPP to protoilludene). MvU portion is composed of MvaE (HMG-CoA reductase/acetyl-CoA acetyltransferase) and MvaS (HMG-CoA synthase). MvL portion is comprised of MvaK1 (mevalonate kinase), MvaK2 (phosphomevalonate kinase), MvaD (diphosphomevalonate decarboxylase) and IDI (IPP isomerase). AO portion consists of IspA (FPP synthase) and OMP7 (protoilludene synthase). Illudins, marasmanes and melleolides are protoilludene derivatives. Pathway intermediates for protoilludene synthesis are as follows: A-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; MVAP, phosphomevalonate; MVAPP, diphosphomevalonate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate; and FPP, farnesyl diphosphate. Solid and dashed arrows indicate the identified and unidentified reactions, respectively
Figure Legend Snippet: Schematic diagram of protoilludene biosynthesis via mevalonate (MVA) pathway. Protoilludene biosynthesis pathway is divided into three portions: MvU (acetyl-CoA to mevalonate), MvL (mevalonate to IPP and DMAPP) and AO (IPP and DMAPP to protoilludene). MvU portion is composed of MvaE (HMG-CoA reductase/acetyl-CoA acetyltransferase) and MvaS (HMG-CoA synthase). MvL portion is comprised of MvaK1 (mevalonate kinase), MvaK2 (phosphomevalonate kinase), MvaD (diphosphomevalonate decarboxylase) and IDI (IPP isomerase). AO portion consists of IspA (FPP synthase) and OMP7 (protoilludene synthase). Illudins, marasmanes and melleolides are protoilludene derivatives. Pathway intermediates for protoilludene synthesis are as follows: A-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; MVAP, phosphomevalonate; MVAPP, diphosphomevalonate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate; and FPP, farnesyl diphosphate. Solid and dashed arrows indicate the identified and unidentified reactions, respectively

Techniques Used:

Optimization of MVA pathway by homolog substitution for the lower portion genes. a Expression constructs of the homolog substituted MvL portions. The homolog genes are from S. aureus and represented with prefixion of “Sa” to the gene name. b and c Effect of combinations of MvU M,H and MvL 7–13 on protoilludene production and mevalonate accumulation. The culture were carried out in 4 mL of 2YT medium containing 2 % (v/v) glycerol with overlay of 1 mL decane at 30 °C for 48 h
Figure Legend Snippet: Optimization of MVA pathway by homolog substitution for the lower portion genes. a Expression constructs of the homolog substituted MvL portions. The homolog genes are from S. aureus and represented with prefixion of “Sa” to the gene name. b and c Effect of combinations of MvU M,H and MvL 7–13 on protoilludene production and mevalonate accumulation. The culture were carried out in 4 mL of 2YT medium containing 2 % (v/v) glycerol with overlay of 1 mL decane at 30 °C for 48 h

Techniques Used: Expressing, Construct

15) Product Images from "Improvement on the productivity of continuous tequila fermentation by Saccharomyces cerevisiae of Agave tequilana juice with supplementation of yeast extract and aeration"

Article Title: Improvement on the productivity of continuous tequila fermentation by Saccharomyces cerevisiae of Agave tequilana juice with supplementation of yeast extract and aeration

Journal: AMB Express

doi: 10.1186/s13568-016-0218-8

Principal component (PC-1 and PC-2) weights of the concentration of aromatic compounds on the steady state of S. cerevisiae continuous cultures supplemented with yeast extract. AC Acetaldehyde, AE Ethyl acetate, ME Methanol, BA 2,3-butanodione, PR Propanol, IB Isobutanol, AI Isoamyl acetate, BU Butanol, IA Isoamyl alcohol, EH Ethyl hexanoate, EO Ethyl octanoate, ED Ethyl decanoate, AT Alpha-terpineol, AF Phenyl acetate, FE 2-phenylethanol
Figure Legend Snippet: Principal component (PC-1 and PC-2) weights of the concentration of aromatic compounds on the steady state of S. cerevisiae continuous cultures supplemented with yeast extract. AC Acetaldehyde, AE Ethyl acetate, ME Methanol, BA 2,3-butanodione, PR Propanol, IB Isobutanol, AI Isoamyl acetate, BU Butanol, IA Isoamyl alcohol, EH Ethyl hexanoate, EO Ethyl octanoate, ED Ethyl decanoate, AT Alpha-terpineol, AF Phenyl acetate, FE 2-phenylethanol

Techniques Used: Concentration Assay, IA

16) Product Images from "An Edible Gintonin Preparation from Ginseng"

Article Title: An Edible Gintonin Preparation from Ginseng

Journal: Journal of Ginseng Research

doi: 10.5142/jgr.2011.35.4.471

GC-MS spectral analysis of lipid components of edible gintonin prepared from ginseng root. Acid hydrolyzed gintonin were partitioned between distilled water and n -butanol. The n -butanol layer, after concentration, was further partitioned between distilled water and n -hexane. The n -hexane layer was subjected to gas chromatography-mass spectrometry with a DB5-MS capillary column. Several major peaks were present in hexane fraction of gintonin and were identified. The 20.94 peak was nonanedioic acid that is known to function to defense after infection in plants. The 22.04 peak of palmitic acid (C16:0) and 23.5 peak of linoleic acid (C18:2) were present as dominant fatty acids or their ester forms.
Figure Legend Snippet: GC-MS spectral analysis of lipid components of edible gintonin prepared from ginseng root. Acid hydrolyzed gintonin were partitioned between distilled water and n -butanol. The n -butanol layer, after concentration, was further partitioned between distilled water and n -hexane. The n -hexane layer was subjected to gas chromatography-mass spectrometry with a DB5-MS capillary column. Several major peaks were present in hexane fraction of gintonin and were identified. The 20.94 peak was nonanedioic acid that is known to function to defense after infection in plants. The 22.04 peak of palmitic acid (C16:0) and 23.5 peak of linoleic acid (C18:2) were present as dominant fatty acids or their ester forms.

Techniques Used: Gas Chromatography-Mass Spectrometry, Concentration Assay, Gas Chromatography, Mass Spectrometry, Infection

17) Product Images from "Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA"

Article Title: Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA

Journal: bioRxiv

doi: 10.1101/2020.08.15.251348

The production of biofuels. 3MB (3‐methyl‐1‐butanol), 2MB (2‐methyl‐1‐ butanol). KIC, 2-ketoisocaproate; KMV, 2-ketomethylvalerate; KDC, 2-keto acid decarboxylase; ADH, alcohol dehydrogenase. (a) Isobutanol (C4) and MB (3MB and 2MB, C5) are produced by synthetic pathway, the essential genes are integrated into three plasmids, they are pSA65, pSA69 and pYX97. (b) The isobutanol production aggregation of strains in panel c to g. **** P
Figure Legend Snippet: The production of biofuels. 3MB (3‐methyl‐1‐butanol), 2MB (2‐methyl‐1‐ butanol). KIC, 2-ketoisocaproate; KMV, 2-ketomethylvalerate; KDC, 2-keto acid decarboxylase; ADH, alcohol dehydrogenase. (a) Isobutanol (C4) and MB (3MB and 2MB, C5) are produced by synthetic pathway, the essential genes are integrated into three plasmids, they are pSA65, pSA69 and pYX97. (b) The isobutanol production aggregation of strains in panel c to g. **** P

Techniques Used: Produced

18) Product Images from "Cytokines Driven Anti-Inflammatory and Anti-Psoriasis Like Efficacies of Nutraceutical Sea Buckthorn (Hippophae rhamnoides) Oil"

Article Title: Cytokines Driven Anti-Inflammatory and Anti-Psoriasis Like Efficacies of Nutraceutical Sea Buckthorn (Hippophae rhamnoides) Oil

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2019.01186

Gas chromatography–flame ionized detector (GC–FID) chromatogram of sea buckthorn oil (SBKT). (A) The fatty acid composition of the SBKT was determined using the GC–FID methodology. Individual fatty acids were identified and quantified using fatty acid methyl ester methodology. Chromatography analysis identified 16 major fatty acids. (B) Pie-diagram represents the percentage of three categories of fatty acids identified in the SBKT—saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) (also see Table 1 ).
Figure Legend Snippet: Gas chromatography–flame ionized detector (GC–FID) chromatogram of sea buckthorn oil (SBKT). (A) The fatty acid composition of the SBKT was determined using the GC–FID methodology. Individual fatty acids were identified and quantified using fatty acid methyl ester methodology. Chromatography analysis identified 16 major fatty acids. (B) Pie-diagram represents the percentage of three categories of fatty acids identified in the SBKT—saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) (also see Table 1 ).

Techniques Used: Gas Chromatography, Chromatography

19) Product Images from "Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging"

Article Title: Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

Journal: Materials

doi: 10.3390/ma10010045

The GC-MSD and GC-FID (insert) chromatograms of thyme oil.
Figure Legend Snippet: The GC-MSD and GC-FID (insert) chromatograms of thyme oil.

Techniques Used:

The GC-MSD and GC-FID (insert) chromatograms of tea tree oil.
Figure Legend Snippet: The GC-MSD and GC-FID (insert) chromatograms of tea tree oil.

Techniques Used:

The GC-MSD and GC-FID (insert) chromatograms of clove oil.
Figure Legend Snippet: The GC-MSD and GC-FID (insert) chromatograms of clove oil.

Techniques Used:

The GC-MSD and GC-FID (insert) chromatograms of rosemary oil.
Figure Legend Snippet: The GC-MSD and GC-FID (insert) chromatograms of rosemary oil.

Techniques Used:

20) Product Images from "Intelligent Poly(N-Isopropylmethacrylamide) Hydrogels: Synthesis, Structure Characterization, Stimuli-Responsive Swelling Properties, and Their Radiation Decomposition"

Article Title: Intelligent Poly(N-Isopropylmethacrylamide) Hydrogels: Synthesis, Structure Characterization, Stimuli-Responsive Swelling Properties, and Their Radiation Decomposition

Journal: Polymers

doi: 10.3390/polym12051112

Comparative view of total ion chromatograms (TIC) of liquid material from irradiated p( N iPMAm) hydrogel volatiles obtained under different oven temperatures.
Figure Legend Snippet: Comparative view of total ion chromatograms (TIC) of liquid material from irradiated p( N iPMAm) hydrogel volatiles obtained under different oven temperatures.

Techniques Used: Irradiation

Related Articles

Gas Chromatography-Mass Spectrometry:

Article Title: Garlic, Onion, and Cinnamon Essential Oil Anti-Biofilms’ Effect against Listeria monocytogenes
Article Snippet: .. Semi-quantification of the volatile substances was performed using gas chromatography coupled to a flame ionization detector (6890 N, Agilent) fitted with the same column and operated under the same conditions as the GC-MS. Quantification was computed as the percentage contribution of each compound to the total amount present. .. Agar Disk Diffusion Assay The disc diffusion method was implemented as described by The European Committee on Antimicrobial Susceptibility Testing [ ] with some modifications, to test the susceptibility of L. monocytogenes to the EOs.

other:

Article Title: Targeted Nutritional Intervention for Patients with Mild Cognitive Impairment: The Cognitive impAiRmEnt Study (CARES) Trial 1
Article Snippet: DHA and EPA Quantification FAME were quantified by GC coupled to flame ionization detector (GC-FID) with an Agilent 7890B Gas Chromatographer, using a Thermo 260M142P column (cyanopropylphenyl-based phase, 30 m length, 0.25 mm inner diameter and 0.25 µm film thickness).

Article Title: Different dietary starch sources alter the carcass traits, meat quality, and the profile of muscle amino acid and fatty acid in finishing pigs
Article Snippet: Fatty acid methyl esters were separated and analyzed using on Agilent 7890B gas chromatographer (GC) system with a flame ionization detector (Agilent Technologies Inc., Santa Clara, CA, USA), using a method described in Li et al. [ ].

Gas Chromatography:

Article Title: Garlic, Onion, and Cinnamon Essential Oil Anti-Biofilms’ Effect against Listeria monocytogenes
Article Snippet: .. Semi-quantification of the volatile substances was performed using gas chromatography coupled to a flame ionization detector (6890 N, Agilent) fitted with the same column and operated under the same conditions as the GC-MS. Quantification was computed as the percentage contribution of each compound to the total amount present. .. Agar Disk Diffusion Assay The disc diffusion method was implemented as described by The European Committee on Antimicrobial Susceptibility Testing [ ] with some modifications, to test the susceptibility of L. monocytogenes to the EOs.

Chromatography:

Article Title: Increased Lipid Accumulation in the Chlamydomonas reinhardtiista7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains ▿
Article Snippet: .. Extracts were washed with distilled water and analyzed directly by gas chromatography-flame ionization detection (GC-FID) using an Agilent 7890A gas chromatograph with a DB5-ms column (Agilent Technologies, Santa Clara, CA). ..

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    Agilent technologies flame ionized detector gc fid
    The <t>GC-MSD</t> and <t>GC-FID</t> (insert) chromatograms of thyme oil.
    Flame Ionized Detector Gc Fid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies gas chromatography flame ionization detector
    The <t>GC-MSD</t> and <t>GC-FID</t> (insert) chromatograms of thyme oil.
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    Agilent technologies gas chromatograph flame ionization detector gc fid
    The <t>GC-MSD</t> and <t>GC-FID</t> (insert) chromatograms of thyme oil.
    Gas Chromatograph Flame Ionization Detector Gc Fid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The GC-MSD and GC-FID (insert) chromatograms of thyme oil.

    Journal: Materials

    Article Title: Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

    doi: 10.3390/ma10010045

    Figure Lengend Snippet: The GC-MSD and GC-FID (insert) chromatograms of thyme oil.

    Article Snippet: Gas Chromatography-Mass Spectrometry Detector and Flame-Ionized Detector Analysis (GC-MSD/FID) The essential oils were characterized by gas chromatography coupled with mass spectrometry detector (GC-MSD) for qualitative analysis and, with a flame ionized detector (GC-FID) (Agilent, Santa Clara, CA, USA), quantitative analysis.

    Techniques:

    The GC-MSD and GC-FID (insert) chromatograms of tea tree oil.

    Journal: Materials

    Article Title: Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

    doi: 10.3390/ma10010045

    Figure Lengend Snippet: The GC-MSD and GC-FID (insert) chromatograms of tea tree oil.

    Article Snippet: Gas Chromatography-Mass Spectrometry Detector and Flame-Ionized Detector Analysis (GC-MSD/FID) The essential oils were characterized by gas chromatography coupled with mass spectrometry detector (GC-MSD) for qualitative analysis and, with a flame ionized detector (GC-FID) (Agilent, Santa Clara, CA, USA), quantitative analysis.

    Techniques:

    The GC-MSD and GC-FID (insert) chromatograms of clove oil.

    Journal: Materials

    Article Title: Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

    doi: 10.3390/ma10010045

    Figure Lengend Snippet: The GC-MSD and GC-FID (insert) chromatograms of clove oil.

    Article Snippet: Gas Chromatography-Mass Spectrometry Detector and Flame-Ionized Detector Analysis (GC-MSD/FID) The essential oils were characterized by gas chromatography coupled with mass spectrometry detector (GC-MSD) for qualitative analysis and, with a flame ionized detector (GC-FID) (Agilent, Santa Clara, CA, USA), quantitative analysis.

    Techniques:

    The GC-MSD and GC-FID (insert) chromatograms of rosemary oil.

    Journal: Materials

    Article Title: Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

    doi: 10.3390/ma10010045

    Figure Lengend Snippet: The GC-MSD and GC-FID (insert) chromatograms of rosemary oil.

    Article Snippet: Gas Chromatography-Mass Spectrometry Detector and Flame-Ionized Detector Analysis (GC-MSD/FID) The essential oils were characterized by gas chromatography coupled with mass spectrometry detector (GC-MSD) for qualitative analysis and, with a flame ionized detector (GC-FID) (Agilent, Santa Clara, CA, USA), quantitative analysis.

    Techniques: