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Varian Medical digesta
Relative abundances of bacterial phyla (p) in the: ( a ) ileal <t>digesta;</t> ( b ) ileal mucosa ( c ); caecal digesta ( d ); and caecal mucosa-associated microbiota of pigs fed the control (CON); flaxseed meal (FM) and oat hulls (OH) diets. The statistical differences were calculated using GLIMMIX procedure and were presented in Supplementry Tables S7 , S8 , S11 and S12 .
Digesta, supplied by Varian Medical, used in various techniques. Bioz Stars score: 92/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Dietary supplementation with flaxseed meal and oat hulls modulates intestinal histomorphometric characteristics, digesta- and mucosa-associated microbiota in pigs"

Article Title: Dietary supplementation with flaxseed meal and oat hulls modulates intestinal histomorphometric characteristics, digesta- and mucosa-associated microbiota in pigs

Journal: Scientific Reports

doi: 10.1038/s41598-018-24043-5

Relative abundances of bacterial phyla (p) in the: ( a ) ileal digesta; ( b ) ileal mucosa ( c ); caecal digesta ( d ); and caecal mucosa-associated microbiota of pigs fed the control (CON); flaxseed meal (FM) and oat hulls (OH) diets. The statistical differences were calculated using GLIMMIX procedure and were presented in Supplementry Tables S7 , S8 , S11 and S12 .
Figure Legend Snippet: Relative abundances of bacterial phyla (p) in the: ( a ) ileal digesta; ( b ) ileal mucosa ( c ); caecal digesta ( d ); and caecal mucosa-associated microbiota of pigs fed the control (CON); flaxseed meal (FM) and oat hulls (OH) diets. The statistical differences were calculated using GLIMMIX procedure and were presented in Supplementry Tables S7 , S8 , S11 and S12 .

Techniques Used:

Predicted functional metagenomes of ileal digesta ( A ) and mucosa ( B ) microbiota of pigs fed control (CON) and flaxseed meal (FM) diets. Linear discriminant analysis (LDA) was performed to identify significant changes in the proportion of reconstructed functional pathways obtained from PICRUSt predictive algorithms at Kyoto Encyclopaedia of Genes and Genomes (KEGG; level 2 and 3). Analysis was performed using linear discriminant analysis of effect size (LEfSe), a metagenome analysis approach which performs the LDA following the Wilcoxon Mann-Whitney test to assess effect size of each differentially abundant variable. Colour code represents the class of treatment. Red indicates variables that were detected as significantly (Log LDA > 2.00) more abundant in CON whereas green indicates variables that were detected as significantly more abundant in FM diet-fed pigs).
Figure Legend Snippet: Predicted functional metagenomes of ileal digesta ( A ) and mucosa ( B ) microbiota of pigs fed control (CON) and flaxseed meal (FM) diets. Linear discriminant analysis (LDA) was performed to identify significant changes in the proportion of reconstructed functional pathways obtained from PICRUSt predictive algorithms at Kyoto Encyclopaedia of Genes and Genomes (KEGG; level 2 and 3). Analysis was performed using linear discriminant analysis of effect size (LEfSe), a metagenome analysis approach which performs the LDA following the Wilcoxon Mann-Whitney test to assess effect size of each differentially abundant variable. Colour code represents the class of treatment. Red indicates variables that were detected as significantly (Log LDA > 2.00) more abundant in CON whereas green indicates variables that were detected as significantly more abundant in FM diet-fed pigs).

Techniques Used: Functional Assay, MANN-WHITNEY

Predicted functional metagenomes of in caecal digesta ( A ) and mucosa ( B ) microbiota of pigs fed the control (CON) and flaxseed meal (FM) diets. Linear discriminant analysis (LDA) was performed to identify significant changes in the proportion of reconstructed functional pathways obtained from PICRUSt predictive algorithms at Kyoto Encyclopaedia of Genes and Genomes (KEGG; level 2 and 3). Analysis was performed using linear discriminant analysis of effect size (LEfSe), a metagenome analysis approach which performs the LDA following the Wilcoxon Mann-Whitney test to assess effect size of each differentially abundant variable. Colour code represents the class of treatment. Red indicates variables that were detected as significantly (Log LDA > 2.00) more abundant in CON, whereas green indicates variables that were detected as significantly more abundant in FM diet-fed pigs. Blue indicates variables that were detected as significantly more abundant in OH-diet fed pigs.
Figure Legend Snippet: Predicted functional metagenomes of in caecal digesta ( A ) and mucosa ( B ) microbiota of pigs fed the control (CON) and flaxseed meal (FM) diets. Linear discriminant analysis (LDA) was performed to identify significant changes in the proportion of reconstructed functional pathways obtained from PICRUSt predictive algorithms at Kyoto Encyclopaedia of Genes and Genomes (KEGG; level 2 and 3). Analysis was performed using linear discriminant analysis of effect size (LEfSe), a metagenome analysis approach which performs the LDA following the Wilcoxon Mann-Whitney test to assess effect size of each differentially abundant variable. Colour code represents the class of treatment. Red indicates variables that were detected as significantly (Log LDA > 2.00) more abundant in CON, whereas green indicates variables that were detected as significantly more abundant in FM diet-fed pigs. Blue indicates variables that were detected as significantly more abundant in OH-diet fed pigs.

Techniques Used: Functional Assay, MANN-WHITNEY

Cluster analysis of microbial communities in the ileal digesta of pigs fed control diet (CON), flaxseed meal (FM) and oat hulls (OH) diets. The sample identifiers on the top branches for diet treatments are coloured. Right branches are coloured to indicate the taxonomical assignment of the OTUs at the phylum level. Each row represents one bacterial taxa (relative abundance above 0.01%), and some taxa could only be classified to family (f), order (o), class (c), or phylum (p) level. The taxa names were labeled by the colour code (magenta) to indicate the abundance of the taxa significantly differed between diet treatments (P
Figure Legend Snippet: Cluster analysis of microbial communities in the ileal digesta of pigs fed control diet (CON), flaxseed meal (FM) and oat hulls (OH) diets. The sample identifiers on the top branches for diet treatments are coloured. Right branches are coloured to indicate the taxonomical assignment of the OTUs at the phylum level. Each row represents one bacterial taxa (relative abundance above 0.01%), and some taxa could only be classified to family (f), order (o), class (c), or phylum (p) level. The taxa names were labeled by the colour code (magenta) to indicate the abundance of the taxa significantly differed between diet treatments (P

Techniques Used: Labeling

Cluster analysis of microbial communities in the caecal digesta of pigs fed control diet (CON), flaxseed meal (FM) and oat hulls (OH) diets. The sample identifiers on the top branches for diet treatments are coloured. Right branches are coloured to indicate the taxonomical assignment of the OTUs at the phylum level. Each row represents one bacterial taxa (relative abundance above 0.01%), and some taxa could only be classified to family (f), order (o), class (c), or phylum (p) level. The taxa names were labeled by the colour code (magenta) to indicate the abundance of the taxa significantly differed between diet treatments (P
Figure Legend Snippet: Cluster analysis of microbial communities in the caecal digesta of pigs fed control diet (CON), flaxseed meal (FM) and oat hulls (OH) diets. The sample identifiers on the top branches for diet treatments are coloured. Right branches are coloured to indicate the taxonomical assignment of the OTUs at the phylum level. Each row represents one bacterial taxa (relative abundance above 0.01%), and some taxa could only be classified to family (f), order (o), class (c), or phylum (p) level. The taxa names were labeled by the colour code (magenta) to indicate the abundance of the taxa significantly differed between diet treatments (P

Techniques Used: Labeling

Non-metric multidimensional scaling (nMDS) ordination plot, illustrating beta biodiversity differences of bacterial community compositions in the caecal digesta (D) and mucosa-associated microbiota (T) of pigs fed control (CON), flaxseed meal (FM) and oat hulls (OH) diets. The PERMANOVA analyses of unweighted UniFrac distances revealed distinct clustering patterns between the ileal digesta microbiota of CON and FM ( P = 0.010), and FM and OH diets-fed pigs ( P = 0.005). The PERMANOVA analysis of unweighted UniFrac distances also revealed distinctions between caecal mucosa-associated microbiota of the CON and FM-diets fed pigs ( P = 0.030), and ileal mucosa-associated microbiota of the FM and OH-diets fed pigs ( P = 0.041).
Figure Legend Snippet: Non-metric multidimensional scaling (nMDS) ordination plot, illustrating beta biodiversity differences of bacterial community compositions in the caecal digesta (D) and mucosa-associated microbiota (T) of pigs fed control (CON), flaxseed meal (FM) and oat hulls (OH) diets. The PERMANOVA analyses of unweighted UniFrac distances revealed distinct clustering patterns between the ileal digesta microbiota of CON and FM ( P = 0.010), and FM and OH diets-fed pigs ( P = 0.005). The PERMANOVA analysis of unweighted UniFrac distances also revealed distinctions between caecal mucosa-associated microbiota of the CON and FM-diets fed pigs ( P = 0.030), and ileal mucosa-associated microbiota of the FM and OH-diets fed pigs ( P = 0.041).

Techniques Used:

2) Product Images from "Isolation of Bacteria Capable of Growth with 2-Methylisoborneol and Geosmin as the Sole Carbon and Energy Sources"

Article Title: Isolation of Bacteria Capable of Growth with 2-Methylisoborneol and Geosmin as the Sole Carbon and Energy Sources

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.06333-11

Phylogenetic tree analysis of 16S rRNA gene sequences of three bacterial isolates found capable of geosmin and MIB biodegradation. Bacteria isolated in the current study are noted with their GenBank accession number. Numbers on branches represent bootstrap tests of phylogeny (500 replicates) using the neighbor-joining with maximum likelihood analysis tool.
Figure Legend Snippet: Phylogenetic tree analysis of 16S rRNA gene sequences of three bacterial isolates found capable of geosmin and MIB biodegradation. Bacteria isolated in the current study are noted with their GenBank accession number. Numbers on branches represent bootstrap tests of phylogeny (500 replicates) using the neighbor-joining with maximum likelihood analysis tool.

Techniques Used: Isolation

MIB (top) and geosmin (bottom) degradation (■) during growth (●) of Rhodococcus sp.-like isolate in mineral media containing MIB or geosmin as the sole carbon source under nonaerated (a) and aerated (b) conditions. Rates are mean values of duplicate analyses. Net loss is depicted relative to abiotic control treatments. Differences between the duplicate determinations did not exceed 10%. Note the use of different time scales for the aerated and nonaerated treatments.
Figure Legend Snippet: MIB (top) and geosmin (bottom) degradation (■) during growth (●) of Rhodococcus sp.-like isolate in mineral media containing MIB or geosmin as the sole carbon source under nonaerated (a) and aerated (b) conditions. Rates are mean values of duplicate analyses. Net loss is depicted relative to abiotic control treatments. Differences between the duplicate determinations did not exceed 10%. Note the use of different time scales for the aerated and nonaerated treatments.

Techniques Used:

Growth of the Rhodococcus sp.-like isolate (expressed as CFU ml −1 ) during incubation in mineral medium under aerobic conditions with geosmin (left) and MIB (right). Initial geosmin and MIB concentrations were 2.5 μg liter −1 (■) and 2.5 mg liter −1 (▲).
Figure Legend Snippet: Growth of the Rhodococcus sp.-like isolate (expressed as CFU ml −1 ) during incubation in mineral medium under aerobic conditions with geosmin (left) and MIB (right). Initial geosmin and MIB concentrations were 2.5 μg liter −1 (■) and 2.5 mg liter −1 (▲).

Techniques Used: Incubation

Geosmin (left) and MIB (right) biodegradation under nonaerated conditions in mineral media containing crude (●) and sterilized (■) sludge obtained from a digestion basin. Rates represent mean values ( n = 3).
Figure Legend Snippet: Geosmin (left) and MIB (right) biodegradation under nonaerated conditions in mineral media containing crude (●) and sterilized (■) sludge obtained from a digestion basin. Rates represent mean values ( n = 3).

Techniques Used:

MIB (top) and geosmin (bottom) degradation (■) during growth (●) of a Variovorax paradoxus -like isolate in mineral media containing MIB or geosmin as the sole carbon source under nonaerated (a) and aerated (b) conditions. Rates are mean values of duplicate analyses. Net loss is depicted relative to abiotic control treatments. Differences between the duplicate determinations did not exceed 10%. Note the use of different time scales for the aerated and nonaerated treatments.
Figure Legend Snippet: MIB (top) and geosmin (bottom) degradation (■) during growth (●) of a Variovorax paradoxus -like isolate in mineral media containing MIB or geosmin as the sole carbon source under nonaerated (a) and aerated (b) conditions. Rates are mean values of duplicate analyses. Net loss is depicted relative to abiotic control treatments. Differences between the duplicate determinations did not exceed 10%. Note the use of different time scales for the aerated and nonaerated treatments.

Techniques Used:

3) Product Images from "Diversity of Saccharomyces cerevisiae Strains Isolated from Two Italian Wine-Producing Regions"

Article Title: Diversity of Saccharomyces cerevisiae Strains Isolated from Two Italian Wine-Producing Regions

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2016.01018

Principal component analysis (PCA) of oenological properties (resistance to SO 2 and copper, fermentative vigor and power, by-products of alcoholic fermentation) of S. cerevisiae isolates (red circle = isolates from Aglianico del Vulture, blue square = isolates from Sangiovese), the scores (A) and variable loadings (B) for the two first principal components. Abbreviations for chemical measures in loadings are: red “A” refers to Aglianico del Vulture, blu “S” refers to Sangiovese, V, fermentative vigor; P, fermentative power; SO 2 , SO 2 resistance; Cu, copper resistance; Et, ethanol; Acetic, acetic acid; meso, meso 2,3 butanediol; racemic, racemic 2,3 butanediol; Gly, glycerol; Aceta, acetaldehyde; Diac, diacetyl; Aceto, acetoin; EA, ethyl acetate; 3-met, 3- methyl 2 butanol; 2-met, 2- methyl 2 butanol; n -butanol, normal butanol; isobut, isobutanol; 1-prop, 1-propanol.
Figure Legend Snippet: Principal component analysis (PCA) of oenological properties (resistance to SO 2 and copper, fermentative vigor and power, by-products of alcoholic fermentation) of S. cerevisiae isolates (red circle = isolates from Aglianico del Vulture, blue square = isolates from Sangiovese), the scores (A) and variable loadings (B) for the two first principal components. Abbreviations for chemical measures in loadings are: red “A” refers to Aglianico del Vulture, blu “S” refers to Sangiovese, V, fermentative vigor; P, fermentative power; SO 2 , SO 2 resistance; Cu, copper resistance; Et, ethanol; Acetic, acetic acid; meso, meso 2,3 butanediol; racemic, racemic 2,3 butanediol; Gly, glycerol; Aceta, acetaldehyde; Diac, diacetyl; Aceto, acetoin; EA, ethyl acetate; 3-met, 3- methyl 2 butanol; 2-met, 2- methyl 2 butanol; n -butanol, normal butanol; isobut, isobutanol; 1-prop, 1-propanol.

Techniques Used:

Related Articles

High Performance Liquid Chromatography:

Article Title: Diversity of Saccharomyces cerevisiae Strains Isolated from Two Italian Wine-Producing Regions
Article Snippet: .. Glucose, fructose, ethanol, glycerol, 2,3-butanediol and acetic acid concentrations in experimental wines were determined by HPLC, according to and , utilizing a MetaCarb H Plus Column (8 μm particle, 300 × 7.8 mm; Varian Inc.) and a Pro-star 210 chromatograph equipped with a Refractive Index Detector, in series (Varian Inc.). .. Higher alcohols (1-propanol, isobutanol, n -butanol, 2-methyl-1-butanol, 3-methyl-1-butanol), acetoin, diacetyl, acetaldehyde and ethyl acetate were analyzed by gas chromatography equipped with glass column (6.6% CW 20M BA 80/120 225, 2 m × 6 × 2 mm) as described by .

Gas Chromatography-Mass Spectrometry:

Article Title: Dietary supplementation with flaxseed meal and oat hulls modulates intestinal histomorphometric characteristics, digesta- and mucosa-associated microbiota in pigs
Article Snippet: .. Thereafter, the digesta samples were analysed for VFA concentration using gas chromatography–mass spectrometry (Varian Chromatograph System, model Star 3400; Varian Medical Systems, Palo Alto, CA, USA) using a capillary column (30 m × 0.5 mm; Restek Corp., Belefonte, PA, USA). ..

Article Title: Isolation of Bacteria Capable of Growth with 2-Methylisoborneol and Geosmin as the Sole Carbon and Energy Sources
Article Snippet: .. Identification of geosmin and MIB peaks detected by GC-FID was verified by parallel analysis of selected samples with a gas chromatograph coupled to a mass spectrometer detector (GC-MS model Saturn 2000; Varian, Inc., Palo Alto, CA). .. The three 16S rRNA gene sequences determined in this study have been deposited in GenBank under accession no. , , and .

Article Title: In Vitro Activity of 30 Essential Oils against Bovine Clinical Isolates of Prototheca zopfii and Prototheca blaschkeae
Article Snippet: .. The gas chromatography–mass spectrometry (GC-MS) analysis was performed using a Varian CP-3800 gas chromatograph equipped with a DB-5 capillary column and a Varian Saturn 2000 ion trap mass detector (Varian Inc., Walnut Creek, CA, USA). ..

Methylation:

Article Title: Effect of Layer-by-Layer (LbL) Encapsulation of Nano-Emulsified Fish Oil on Their Digestibility Ex Vivo and Skin Permeability In Vitro
Article Snippet: .. Fatty acid analysis The fatty acid methyl esters (FAMEs) were prepared by saponification and methylation ( ) and analyzed in a gas chromatograph (Varian 3900 GC, Varian, Inc., Walnut Creek, CA, USA) equipped with a supelco-2560 column (length 100 m, ID 0.25 mm, film thickness 0.2 μm) with helium carrier gas from 100°C increased at 3°C/min to 240°C. .. Injection temperature and the flame-ionization detector temperatures were set at 225°C and 270°C, respectively.

Concentration Assay:

Article Title: Dietary supplementation with flaxseed meal and oat hulls modulates intestinal histomorphometric characteristics, digesta- and mucosa-associated microbiota in pigs
Article Snippet: .. Thereafter, the digesta samples were analysed for VFA concentration using gas chromatography–mass spectrometry (Varian Chromatograph System, model Star 3400; Varian Medical Systems, Palo Alto, CA, USA) using a capillary column (30 m × 0.5 mm; Restek Corp., Belefonte, PA, USA). ..

Mass Spectrometry:

Article Title: Isolation of Bacteria Capable of Growth with 2-Methylisoborneol and Geosmin as the Sole Carbon and Energy Sources
Article Snippet: .. Identification of geosmin and MIB peaks detected by GC-FID was verified by parallel analysis of selected samples with a gas chromatograph coupled to a mass spectrometer detector (GC-MS model Saturn 2000; Varian, Inc., Palo Alto, CA). .. The three 16S rRNA gene sequences determined in this study have been deposited in GenBank under accession no. , , and .

Injection:

Article Title: Chemical Transfers Occurring Through Oenococcus oeni Biofilm in Different Enological Conditions
Article Snippet: .. Individual alcohols were determined by GC-FID (Varian, Inc.), using a direct 1/1,000 split injection of 1 μL of the sample into a CP-WAX 50 × 32 capillary column in the presence of octan-2-ol as internal standard. ..

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    Varian Medical gc ms conditions gc ms analysis
    <t>GC/MS</t> chromatogram and EI mass spectrum of the (a) fluoride- and (b) acetate-2,3,4,5,6-pentafluorobenzyl bromide ester derivatives.
    Gc Ms Conditions Gc Ms Analysis, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gc ms conditions gc ms analysis - by Bioz Stars, 2020-09
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    Varian Medical mass spectrometry gc ms
    <t>GC/MS</t> chromatogram and EI mass spectrum of the (a) fluoride- and (b) acetate-2,3,4,5,6-pentafluorobenzyl bromide ester derivatives.
    Mass Spectrometry Gc Ms, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mass spectrometry gc ms/product/Varian Medical
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    92
    Varian Medical gc ms analysis
    <t>GC/MS</t> chromatogram and EI mass spectrum of the (a) fluoride- and (b) acetate-2,3,4,5,6-pentafluorobenzyl bromide ester derivatives.
    Gc Ms Analysis, supplied by Varian Medical, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc ms analysis/product/Varian Medical
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    Varian Medical tandem gas chromatography mass spectrometry gc msms
    <t>GC/MS</t> chromatogram and EI mass spectrum of the (a) fluoride- and (b) acetate-2,3,4,5,6-pentafluorobenzyl bromide ester derivatives.
    Tandem Gas Chromatography Mass Spectrometry Gc Msms, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tandem gas chromatography mass spectrometry gc msms/product/Varian Medical
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    Image Search Results


    GC/MS chromatogram and EI mass spectrum of the (a) fluoride- and (b) acetate-2,3,4,5,6-pentafluorobenzyl bromide ester derivatives.

    Journal: International Journal of Analytical Chemistry

    Article Title: Innovative Monitoring of Atmospheric Gaseous Hydrogen Fluoride

    doi: 10.1155/2016/2129053

    Figure Lengend Snippet: GC/MS chromatogram and EI mass spectrum of the (a) fluoride- and (b) acetate-2,3,4,5,6-pentafluorobenzyl bromide ester derivatives.

    Article Snippet: GC/MS Conditions GC/MS analysis was performed using a Varian CP-3800 GC equipped with an electronic flow control (Varian Inc., Palo Alto, CA, USA).

    Techniques: Gas Chromatography-Mass Spectrometry