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Agilent technologies gas chromatographer
Gas Chromatographer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gas chromatographer/product/Agilent technologies
Average 91 stars, based on 27 article reviews
Price from $9.99 to $1999.99
gas chromatographer - by Bioz Stars, 2020-09
91/100 stars

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Gas Chromatography-Mass Spectrometry:

Article Title: Antifungal activity of Gallesia integrifolia fruit essential oil
Article Snippet: .. Chemical identification of the essential oil occurred by using a gas chromatographer coupled to a mass spectrometer (GC–MS; Agilent 19091J-433). ..

Article Title: The Involvement of Nitric Oxide in Integration of Plant Physiological and Ultrastructural Adjustments in Response to Arsenic
Article Snippet: .. The GC–MS system used was composed of autosampler CTC CombiPAL, gas chromatographer (Agilent 6890N) and mass spectrometry (Leco Pegasus III TOF-MS, operated in positive ionization mode). ..

Injection:

Article Title: Pleurotus Mushrooms Content in Glucans and Ergosterol Assessed by ATR-FTIR Spectroscopy and Multivariate Analysis
Article Snippet: .. Sterols were derivatized to trimethylsilylethers (TMS) with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) at 70 °C for 20 min, and 1 μL aliquots were injected in the gas chromatographer (Agilent HP GC 6890 N; Wallborn, Germany) coupled with a mass spectrometer (Agilent HP 5973; Wallborn, Germany) at a split ratio of 5:1. .. The analysis of the TMS sterol derivatives was carried out under electron impact ionization (70 eV) and separated by an Agilent J & W HP-5ms capillary column (30 m × 0.25 mm × 250 μm) with a carrier gas flow rate equal to 0.6 mL/min (high-purity He).

Article Title: Supported Bimetallic AuPd Nanoparticles as a Catalyst for the Selective Hydrogenation of Nitroarenes
Article Snippet: .. The reaction mixture was then taken out, centrifuged to remove the solid catalyst, and filtered before being injected into a gas-chromatographer (Varian 450-GC, Agilent, Santa Clara, CA, USA) equipped with a flame ionization detector (FID), a methanizer and a CP-SiL5CB column (50 m, 0.33 mm, Thermo Fisher Scientific, Newport, UK), in which He was used as the carrier gas. ..

other:

Article Title: Supported Bimetallic AuPd Nanoparticles as a Catalyst for the Selective Hydrogenation of Nitroarenes
Article Snippet: For the solvent free reaction, an equal amount of reaction mixture (0.5 mL) and external standard o -xylene (0.5 mL) were added and accurately weighted in a gas chromatographer (GC)vial.

Solid-phase Microextraction:

Article Title: Isolation and Characterization of Live Yeast Cells from Ancient Vessels as a Tool in Bio-Archaeology
Article Snippet: .. Qualitative analysis of relative peak areas for each compound across our samples was conducted using SPME Fiber (DVB/CAR/PDMS), followed by separation by gas chromatographer and detection by Agilent 5973 mass spectrometer (MS) detector in Full-Scan mode. .. Suggestions for the identification of the detected peaks were carried out by Wiley mass spectrometry database.

Mass Spectrometry:

Article Title: Pleurotus Mushrooms Content in Glucans and Ergosterol Assessed by ATR-FTIR Spectroscopy and Multivariate Analysis
Article Snippet: .. Sterols were derivatized to trimethylsilylethers (TMS) with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) at 70 °C for 20 min, and 1 μL aliquots were injected in the gas chromatographer (Agilent HP GC 6890 N; Wallborn, Germany) coupled with a mass spectrometer (Agilent HP 5973; Wallborn, Germany) at a split ratio of 5:1. .. The analysis of the TMS sterol derivatives was carried out under electron impact ionization (70 eV) and separated by an Agilent J & W HP-5ms capillary column (30 m × 0.25 mm × 250 μm) with a carrier gas flow rate equal to 0.6 mL/min (high-purity He).

Article Title: Isolation and Characterization of Live Yeast Cells from Ancient Vessels as a Tool in Bio-Archaeology
Article Snippet: .. Qualitative analysis of relative peak areas for each compound across our samples was conducted using SPME Fiber (DVB/CAR/PDMS), followed by separation by gas chromatographer and detection by Agilent 5973 mass spectrometer (MS) detector in Full-Scan mode. .. Suggestions for the identification of the detected peaks were carried out by Wiley mass spectrometry database.

Article Title: Antifungal activity of Gallesia integrifolia fruit essential oil
Article Snippet: .. Chemical identification of the essential oil occurred by using a gas chromatographer coupled to a mass spectrometer (GC–MS; Agilent 19091J-433). ..

Article Title: The Involvement of Nitric Oxide in Integration of Plant Physiological and Ultrastructural Adjustments in Response to Arsenic
Article Snippet: .. The GC–MS system used was composed of autosampler CTC CombiPAL, gas chromatographer (Agilent 6890N) and mass spectrometry (Leco Pegasus III TOF-MS, operated in positive ionization mode). ..

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  • 93
    Agilent technologies t asperellum gc ms
    Phenotypical characteristics of T. <t>asperellum</t> . ( a ) Culture plate. ( b ) Microscopic view of the lactophenol cotton blue mount of T. asperellum (TaspSKGN2) (10x).
    T Asperellum Gc Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t asperellum gc ms/product/Agilent technologies
    Average 93 stars, based on 1 article reviews
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    92
    Agilent technologies gc ms detection gc ms detection
    Evaluation of <t>GC-MS</t> <t>detection</t> model through the PCA scores plot of QC samples. Gathered points of QC sample showed that the PCA model was reliable.
    Gc Ms Detection Gc Ms Detection, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc ms detection gc ms detection/product/Agilent technologies
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    88
    Agilent technologies mouse pgc 1α
    Cellular respiration in neurons over-expressing <t>PGC-1α.</t> ( A – D ) Primary neuronal cultures were infected with either the NCV or the PGC1 vector at day 7. Analysis of cellular respiration was performed at 5 (A) and 7 (C) days post-infection. OCRs were measured on 10 wells per group in basal conditions during 60 min. In each group, a subset of four wells was then treated with 5 µ m oligomycin (ATP synthase inhibitor) and OCR was measured during 60 min. Finally, another subset of five wells per group was exposed to 20 µ m FCCP (mitochondrial protonophore) and the OCR was measured during 30 min. (B and D) Based on these measurements, we assessed the following OCRs: basal ( n = 10), dedicated to ATP production ( n = 4), in presence of FCCP ( n = 5) and reserve capacity ( n = 5). Student's t -test: ** P
    Mouse Pgc 1α, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse pgc 1α/product/Agilent technologies
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    84
    Agilent technologies gc ms metabolomics data data
    Transcriptome and <t>metabolomics</t> analysis of brown fat shows increased carbohydrate metabolism during cold exposure. (a) Atom mapping for [U- 13 C]glucose tracing into glycolysis and the TCA cycle. White balls are 12 C atoms. Black balls are 13 C atoms. (b) Tracing analysis from U- 13 C glucose in differentiated brown adipocytes treated with vehicle or 100nM CL-316,243 for 5 hours (N=3).
    Gc Ms Metabolomics Data Data, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Phenotypical characteristics of T. asperellum . ( a ) Culture plate. ( b ) Microscopic view of the lactophenol cotton blue mount of T. asperellum (TaspSKGN2) (10x).

    Journal: Scientific Reports

    Article Title: A new application of Trichoderma asperellum as an anopheline larvicide for eco friendly management in medical science

    doi: 10.1038/s41598-018-37108-2

    Figure Lengend Snippet: Phenotypical characteristics of T. asperellum . ( a ) Culture plate. ( b ) Microscopic view of the lactophenol cotton blue mount of T. asperellum (TaspSKGN2) (10x).

    Article Snippet: Gas Chromatography-Mass spectrometry (GC-MS) analysis of crude methanolic extract of T. asperellum GC-MS was performed by using an Agilent Technologies, GC-6860N Network GC System with a 5973 inert Mass Selective Detector and the search library used was NBS75K.L .

    Techniques:

    Thin layer chromatography and GC-MS study of fungal ME, and its effect in reduction of the phenoloxidase inside the anopheline larvae. ( a ) Spots of the MF8 of T. asperellum (TaspSKGN2) in TLC plates under UV (254 nm 366 nm) and within an iodine vapor chamber. ( Rf value: 0.83) [i. Spot under UV at 254 nm ii. Spot under UV at 366 nm iii. Spot within Iodine vapour chamber]. ( b ) GC-MS chromatogram of the MF8 of T. asperellum ME extracts exhibiting peaks of 49 compounds. ( c ) Graphical representation of the reduction in phenoloxidase activity after the initial increase inside the anopheline larvae (N = 10) treated with the LD 50 dose of ME of T. asperellum in different interaction times.

    Journal: Scientific Reports

    Article Title: A new application of Trichoderma asperellum as an anopheline larvicide for eco friendly management in medical science

    doi: 10.1038/s41598-018-37108-2

    Figure Lengend Snippet: Thin layer chromatography and GC-MS study of fungal ME, and its effect in reduction of the phenoloxidase inside the anopheline larvae. ( a ) Spots of the MF8 of T. asperellum (TaspSKGN2) in TLC plates under UV (254 nm 366 nm) and within an iodine vapor chamber. ( Rf value: 0.83) [i. Spot under UV at 254 nm ii. Spot under UV at 366 nm iii. Spot within Iodine vapour chamber]. ( b ) GC-MS chromatogram of the MF8 of T. asperellum ME extracts exhibiting peaks of 49 compounds. ( c ) Graphical representation of the reduction in phenoloxidase activity after the initial increase inside the anopheline larvae (N = 10) treated with the LD 50 dose of ME of T. asperellum in different interaction times.

    Article Snippet: Gas Chromatography-Mass spectrometry (GC-MS) analysis of crude methanolic extract of T. asperellum GC-MS was performed by using an Agilent Technologies, GC-6860N Network GC System with a 5973 inert Mass Selective Detector and the search library used was NBS75K.L .

    Techniques: Thin Layer Chromatography, Gas Chromatography-Mass Spectrometry, Activity Assay

    T. asperellum (TaspSKGN2) spores and ME treated dead Anopheles 3 rd instar larvae (microscopic view). ( a ) TaspSKGN2 spores (lactophenol cotton blue stained) attached on the outer body surface and blocking spiracles of the treated larvae (4x). ( b , c ) Hyphal outgrowth from the inner surface of the infected larvae (10x and 40x, respectively]. ( d ) Non treated larvae stained with alizarine (10x) e. Tissue degeneration of the ME treated larvae (red marked area) stained with alizarine (10x).

    Journal: Scientific Reports

    Article Title: A new application of Trichoderma asperellum as an anopheline larvicide for eco friendly management in medical science

    doi: 10.1038/s41598-018-37108-2

    Figure Lengend Snippet: T. asperellum (TaspSKGN2) spores and ME treated dead Anopheles 3 rd instar larvae (microscopic view). ( a ) TaspSKGN2 spores (lactophenol cotton blue stained) attached on the outer body surface and blocking spiracles of the treated larvae (4x). ( b , c ) Hyphal outgrowth from the inner surface of the infected larvae (10x and 40x, respectively]. ( d ) Non treated larvae stained with alizarine (10x) e. Tissue degeneration of the ME treated larvae (red marked area) stained with alizarine (10x).

    Article Snippet: Gas Chromatography-Mass spectrometry (GC-MS) analysis of crude methanolic extract of T. asperellum GC-MS was performed by using an Agilent Technologies, GC-6860N Network GC System with a 5973 inert Mass Selective Detector and the search library used was NBS75K.L .

    Techniques: Staining, Blocking Assay, Infection

    Mass spore production of T. asperellum (TaspSKGN2). ( a ) Spore production (mean ± SD) by Trichoderma asperellum (TaspSKGN2) in different natural media from three replicates (tabular form). ( b ) Photographs of mass spore production in different natural media (after 15 d of inoculation): i. Corn medium, ii. Paddy seed medium, iii. Rice husk medium, iv. Wheat medium, v. Sugarcane bagasse medium.

    Journal: Scientific Reports

    Article Title: A new application of Trichoderma asperellum as an anopheline larvicide for eco friendly management in medical science

    doi: 10.1038/s41598-018-37108-2

    Figure Lengend Snippet: Mass spore production of T. asperellum (TaspSKGN2). ( a ) Spore production (mean ± SD) by Trichoderma asperellum (TaspSKGN2) in different natural media from three replicates (tabular form). ( b ) Photographs of mass spore production in different natural media (after 15 d of inoculation): i. Corn medium, ii. Paddy seed medium, iii. Rice husk medium, iv. Wheat medium, v. Sugarcane bagasse medium.

    Article Snippet: Gas Chromatography-Mass spectrometry (GC-MS) analysis of crude methanolic extract of T. asperellum GC-MS was performed by using an Agilent Technologies, GC-6860N Network GC System with a 5973 inert Mass Selective Detector and the search library used was NBS75K.L .

    Techniques:

    Isolation of T. asperellum from soil. ( i ) Mosquito baiting technique a. Inoculation of dead adult mosquito b. Growth of fungus upon mosquitoes c. Transfer of fungi infected mosquitoes to a petridish containing PDA d. Growth of fungus in PDA medium. ( ii ) Soil dilution technique a. Soil sample b. Growth of several fungal colonies in the soil dilution plate.

    Journal: Scientific Reports

    Article Title: A new application of Trichoderma asperellum as an anopheline larvicide for eco friendly management in medical science

    doi: 10.1038/s41598-018-37108-2

    Figure Lengend Snippet: Isolation of T. asperellum from soil. ( i ) Mosquito baiting technique a. Inoculation of dead adult mosquito b. Growth of fungus upon mosquitoes c. Transfer of fungi infected mosquitoes to a petridish containing PDA d. Growth of fungus in PDA medium. ( ii ) Soil dilution technique a. Soil sample b. Growth of several fungal colonies in the soil dilution plate.

    Article Snippet: Gas Chromatography-Mass spectrometry (GC-MS) analysis of crude methanolic extract of T. asperellum GC-MS was performed by using an Agilent Technologies, GC-6860N Network GC System with a 5973 inert Mass Selective Detector and the search library used was NBS75K.L .

    Techniques: Isolation, Infection

    Evaluation of GC-MS detection model through the PCA scores plot of QC samples. Gathered points of QC sample showed that the PCA model was reliable.

    Journal: Polymers

    Article Title: Therapeutic Effect and Metabolic Mechanism of A Selenium-Polysaccharide from Ziyang Green Tea on Chronic Fatigue Syndrome

    doi: 10.3390/polym10111269

    Figure Lengend Snippet: Evaluation of GC-MS detection model through the PCA scores plot of QC samples. Gathered points of QC sample showed that the PCA model was reliable.

    Article Snippet: GC-MS Detection GC-MS detection was performed using a gas chromatograph system (Agilent 7890, San Francisco, CA, USA) and a mass spectrometer (Pegasus HT, Hazel Grove, UK).

    Techniques: Gas Chromatography-Mass Spectrometry

    Cellular respiration in neurons over-expressing PGC-1α. ( A – D ) Primary neuronal cultures were infected with either the NCV or the PGC1 vector at day 7. Analysis of cellular respiration was performed at 5 (A) and 7 (C) days post-infection. OCRs were measured on 10 wells per group in basal conditions during 60 min. In each group, a subset of four wells was then treated with 5 µ m oligomycin (ATP synthase inhibitor) and OCR was measured during 60 min. Finally, another subset of five wells per group was exposed to 20 µ m FCCP (mitochondrial protonophore) and the OCR was measured during 30 min. (B and D) Based on these measurements, we assessed the following OCRs: basal ( n = 10), dedicated to ATP production ( n = 4), in presence of FCCP ( n = 5) and reserve capacity ( n = 5). Student's t -test: ** P

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Cellular respiration in neurons over-expressing PGC-1α. ( A – D ) Primary neuronal cultures were infected with either the NCV or the PGC1 vector at day 7. Analysis of cellular respiration was performed at 5 (A) and 7 (C) days post-infection. OCRs were measured on 10 wells per group in basal conditions during 60 min. In each group, a subset of four wells was then treated with 5 µ m oligomycin (ATP synthase inhibitor) and OCR was measured during 60 min. Finally, another subset of five wells per group was exposed to 20 µ m FCCP (mitochondrial protonophore) and the OCR was measured during 30 min. (B and D) Based on these measurements, we assessed the following OCRs: basal ( n = 10), dedicated to ATP production ( n = 4), in presence of FCCP ( n = 5) and reserve capacity ( n = 5). Student's t -test: ** P

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Expressing, Pyrolysis Gas Chromatography, Infection, Plasmid Preparation

    Overexpression of PGC-1α and long-term effects in vivo. ( A ) Nigral PGC-1α immunostaining at 3 months post-injection of an AAV2/6-PGC1 vector directly in the rat SNpc. ( B ) Striatal PGC-1α immunostaining at 3 months post-injection of an AAV2/6-PGC1 vector in the rat striatum. ( C ) HSP60 immunostaining showing mitochondrial biogenesis in the striatum of a rat injected with an AAV2/6-PGC1 in the striatum. For each immunostaining, the non-injected side (NInjS) is shown for comparison. Scale bar: 100 µm.

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Overexpression of PGC-1α and long-term effects in vivo. ( A ) Nigral PGC-1α immunostaining at 3 months post-injection of an AAV2/6-PGC1 vector directly in the rat SNpc. ( B ) Striatal PGC-1α immunostaining at 3 months post-injection of an AAV2/6-PGC1 vector in the rat striatum. ( C ) HSP60 immunostaining showing mitochondrial biogenesis in the striatum of a rat injected with an AAV2/6-PGC1 in the striatum. For each immunostaining, the non-injected side (NInjS) is shown for comparison. Scale bar: 100 µm.

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Over Expression, Pyrolysis Gas Chromatography, In Vivo, Immunostaining, Injection, Plasmid Preparation

    Mitochondrial biogenesis in neurons over-expressing PGC-1α. Primary neuronal culture from mouse ventral midbrain was cultured for 7 days and then transduced with AAV2/6 encoding either PGC1 or GFP. Three days later, the neurons were infected with a vector encoding the MitoDsRed fluorescent protein and analyzed by flow cytometry. Control conditions include non-infected (NI) neurons and neurons infected with the MitoDsRed (M) vector only. ( A ) Representative flow cytometry dot plots: living cells were gated in region R1 of the FS/SS plot. Average Cy5 fluorescence intensity was determined for each group on cells gated in region R2, identified as the population of neurons expressing the MitoDsRed fluorescent protein. ( B ) PGC1 neurons show a clear increase in Cy5 fluorescence reflecting mitochondrial biogenesis. One-way ANOVA with Newman–Keuls post hoc test: NI, n = 2; M, n = 2; GFP, n = 3; PGC1, n = 3; * P

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Mitochondrial biogenesis in neurons over-expressing PGC-1α. Primary neuronal culture from mouse ventral midbrain was cultured for 7 days and then transduced with AAV2/6 encoding either PGC1 or GFP. Three days later, the neurons were infected with a vector encoding the MitoDsRed fluorescent protein and analyzed by flow cytometry. Control conditions include non-infected (NI) neurons and neurons infected with the MitoDsRed (M) vector only. ( A ) Representative flow cytometry dot plots: living cells were gated in region R1 of the FS/SS plot. Average Cy5 fluorescence intensity was determined for each group on cells gated in region R2, identified as the population of neurons expressing the MitoDsRed fluorescent protein. ( B ) PGC1 neurons show a clear increase in Cy5 fluorescence reflecting mitochondrial biogenesis. One-way ANOVA with Newman–Keuls post hoc test: NI, n = 2; M, n = 2; GFP, n = 3; PGC1, n = 3; * P

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Expressing, Pyrolysis Gas Chromatography, Cell Culture, Transduction, Infection, Plasmid Preparation, Flow Cytometry, Cytometry, Fluorescence

    PGC-1α up-regulates PV expression in the substantia nigra and striatum. Immunostaining demonstrating an increase in the amount of PV-positive neurons in the injected SNpc and reticulata of rats injected with AAV2/6-PGC1 in the SNpc ( A ). No change in PV expression in rats injected with a non-coding vector in the SNpc ( B ). PGC1 and NCV Hi; scale bar: 200 μm. A clear up-regulation of the striatal interneuron marker PV is found in rats injected with AAV2/6-PGC1 in the striatum ( C ), in contrast to rats similarly injected with a non-coding vector ( D ). PGC1 and NCV Lo; scale bar: 250 μm.

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: PGC-1α up-regulates PV expression in the substantia nigra and striatum. Immunostaining demonstrating an increase in the amount of PV-positive neurons in the injected SNpc and reticulata of rats injected with AAV2/6-PGC1 in the SNpc ( A ). No change in PV expression in rats injected with a non-coding vector in the SNpc ( B ). PGC1 and NCV Hi; scale bar: 200 μm. A clear up-regulation of the striatal interneuron marker PV is found in rats injected with AAV2/6-PGC1 in the striatum ( C ), in contrast to rats similarly injected with a non-coding vector ( D ). PGC1 and NCV Lo; scale bar: 250 μm.

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Immunostaining, Injection, Plasmid Preparation, Marker

    Loss of dopaminergic nigrostriatal markers in response to PGC-1α overexpression. ( A and B ) Loss of VMAT2-positive (A) and TH-positive (B) neurons in the SNpc of rats displaying either a moderate (PGC1 Lo) or a high level of PGC-1α overexpression (PGC1 Hi) in the SNpc at 3 months post-injection. Note the overt loss of VMAT2 and TH neurons in the PGC1 Hi condition. ( C and D ) Loss of striatal VMAT2 (C) and TH (D) immunoreactivity in PGC1 Lo and PGC1 Hi rats at 3 months post-injection. Note in both conditions, the loss of striatal dopaminergic markers. ( E and F ) Representative photomicrographs showing VMAT2-positive neurons in the SNpc of PGC1 Hi rats (E) and PGC1 Lo rats (F), when compared with the non-injected side (NInjS); scale bar: 100 µm. ( G and I ) Representative photomicrographs showing the loss of VMAT2 marker in the striatum of PGC1 Hi (G) and PGC1 Lo rats (I). In both conditions, striatal DARPP32 immunoreactivity remains intact ( H and J —corresponding to PGC1 Hi and PGC1 Lo, respectively). ( K ) Stereological quantification of the percentage loss of Nissl-positive neurons in the SNpc.

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Loss of dopaminergic nigrostriatal markers in response to PGC-1α overexpression. ( A and B ) Loss of VMAT2-positive (A) and TH-positive (B) neurons in the SNpc of rats displaying either a moderate (PGC1 Lo) or a high level of PGC-1α overexpression (PGC1 Hi) in the SNpc at 3 months post-injection. Note the overt loss of VMAT2 and TH neurons in the PGC1 Hi condition. ( C and D ) Loss of striatal VMAT2 (C) and TH (D) immunoreactivity in PGC1 Lo and PGC1 Hi rats at 3 months post-injection. Note in both conditions, the loss of striatal dopaminergic markers. ( E and F ) Representative photomicrographs showing VMAT2-positive neurons in the SNpc of PGC1 Hi rats (E) and PGC1 Lo rats (F), when compared with the non-injected side (NInjS); scale bar: 100 µm. ( G and I ) Representative photomicrographs showing the loss of VMAT2 marker in the striatum of PGC1 Hi (G) and PGC1 Lo rats (I). In both conditions, striatal DARPP32 immunoreactivity remains intact ( H and J —corresponding to PGC1 Hi and PGC1 Lo, respectively). ( K ) Stereological quantification of the percentage loss of Nissl-positive neurons in the SNpc.

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Pyrolysis Gas Chromatography, Over Expression, Injection, Marker

    Loss of dopaminergic markers and behavioral assessment in response to PGC-1α overexpression in rats expressing human αSyn. Rats were injected in the SNpc with AAV2/6 vectors encoding either human αSynWT or the A53T mutant. The animals were simultaneously injected in the striatum with an AAV2/6 vector encoding PGC-1α or a NCV to ensure a moderate expression in the SNpc. ( A – C ) To assess motor asymmetry, forepaw activity was measured in the cylinder test (A and B) and ipsiversive rotations were measured following amphetamine administration (C). In the cylinder test, note the progressive development of an asymmetry in forepaw use typically observed following unilateral expression of αSynWT or A53T (repeated measure ANOVA, time effect: P

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Loss of dopaminergic markers and behavioral assessment in response to PGC-1α overexpression in rats expressing human αSyn. Rats were injected in the SNpc with AAV2/6 vectors encoding either human αSynWT or the A53T mutant. The animals were simultaneously injected in the striatum with an AAV2/6 vector encoding PGC-1α or a NCV to ensure a moderate expression in the SNpc. ( A – C ) To assess motor asymmetry, forepaw activity was measured in the cylinder test (A and B) and ipsiversive rotations were measured following amphetamine administration (C). In the cylinder test, note the progressive development of an asymmetry in forepaw use typically observed following unilateral expression of αSynWT or A53T (repeated measure ANOVA, time effect: P

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Pyrolysis Gas Chromatography, Over Expression, Expressing, Injection, Mutagenesis, Plasmid Preparation, Activity Assay

    Absence of fluorogold retrograde labeling in nigral dopaminergic neurons overexpressing PGC-1α. Immunostaining for TH expression and fluorogold uptake at 3 months post-injection of either PGC-1α or non-coding vector. Note the absence of fluorogold signal in the SNpc of PGC1 Lo rats, indicative of impaired uptake or retrograde transport of the tracer. PGC1 and NCV Lo; scale bar: 100 μm.

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Absence of fluorogold retrograde labeling in nigral dopaminergic neurons overexpressing PGC-1α. Immunostaining for TH expression and fluorogold uptake at 3 months post-injection of either PGC-1α or non-coding vector. Note the absence of fluorogold signal in the SNpc of PGC1 Lo rats, indicative of impaired uptake or retrograde transport of the tracer. PGC1 and NCV Lo; scale bar: 100 μm.

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Labeling, Pyrolysis Gas Chromatography, Immunostaining, Expressing, Injection, Plasmid Preparation

    Mitochondrial transcriptome analysis of neuronal cultures overexpressing PGC-1α. Seven-day-old primary neuronal cultures from mouse ventral midbrain were infected with either a NCV or a vector encoding PGC-1α (PGC1). PCR arrays were performed at days 5 ( A ) and 7 ( B ) post-infection, to measure changes in the expression of 84 nuclear genes involved in various mitochondrial functions. Dark grey columns indicate significant changes in gene expression ( n = 4, Student's t -test, P

    Journal: Human Molecular Genetics

    Article Title: Sustained expression of PGC-1? in the rat nigrostriatal system selectively impairs dopaminergic function

    doi: 10.1093/hmg/ddr618

    Figure Lengend Snippet: Mitochondrial transcriptome analysis of neuronal cultures overexpressing PGC-1α. Seven-day-old primary neuronal cultures from mouse ventral midbrain were infected with either a NCV or a vector encoding PGC-1α (PGC1). PCR arrays were performed at days 5 ( A ) and 7 ( B ) post-infection, to measure changes in the expression of 84 nuclear genes involved in various mitochondrial functions. Dark grey columns indicate significant changes in gene expression ( n = 4, Student's t -test, P

    Article Snippet: Plasmid construction Human WT (nucleotides 46–520, GeneBank accession no. NM_000345) and A53T αSyn and mouse PGC-1α (nucleotides 35–2428, GeneBank accession no. BC066868) were inserted into the pAAV-pgk-MCS backbone, modified from the serotype 2 pAAV-cmv-MCS (Agilent, La Jolla, CA, USA) using standard cloning procedures.

    Techniques: Pyrolysis Gas Chromatography, Infection, Plasmid Preparation, Polymerase Chain Reaction, Expressing

    Transcriptome and metabolomics analysis of brown fat shows increased carbohydrate metabolism during cold exposure. (a) Atom mapping for [U- 13 C]glucose tracing into glycolysis and the TCA cycle. White balls are 12 C atoms. Black balls are 13 C atoms. (b) Tracing analysis from U- 13 C glucose in differentiated brown adipocytes treated with vehicle or 100nM CL-316,243 for 5 hours (N=3).

    Journal: bioRxiv

    Article Title: Mitochondrial pyruvate carrier is required for optimal brown fat thermogenesis

    doi: 10.1101/823799

    Figure Lengend Snippet: Transcriptome and metabolomics analysis of brown fat shows increased carbohydrate metabolism during cold exposure. (a) Atom mapping for [U- 13 C]glucose tracing into glycolysis and the TCA cycle. White balls are 12 C atoms. Black balls are 13 C atoms. (b) Tracing analysis from U- 13 C glucose in differentiated brown adipocytes treated with vehicle or 100nM CL-316,243 for 5 hours (N=3).

    Article Snippet: Analysis of GC-MS metabolomics data Data was collected using MassHunter software (Agilent).

    Techniques:

    Transcriptome and metabolomics analysis of brown fat shows increased carbohydrate metabolism and glycolytic metabolism during cold exposure. (a) Network visualization of enriched biological pathways altered with cold exposure in BAT (N=5). (b) GSEA pathway analysis of differentially expressing genes (FDR

    Journal: bioRxiv

    Article Title: Mitochondrial pyruvate carrier is required for optimal brown fat thermogenesis

    doi: 10.1101/823799

    Figure Lengend Snippet: Transcriptome and metabolomics analysis of brown fat shows increased carbohydrate metabolism and glycolytic metabolism during cold exposure. (a) Network visualization of enriched biological pathways altered with cold exposure in BAT (N=5). (b) GSEA pathway analysis of differentially expressing genes (FDR

    Article Snippet: Analysis of GC-MS metabolomics data Data was collected using MassHunter software (Agilent).

    Techniques: Expressing