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PerkinElmer gas chromatograph
Gas Chromatograph, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gas chromatograph - by Bioz Stars, 2020-09
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Mass Spectrometry:

Article Title: The Influence of Prior Learning Experience on Pollinator Choice: An Experiment Using Bumblebees on Two Wild Floral Types of Antirrhinum majus
Article Snippet: .. VOC samples were thermodesorbed (cool trap -30 to 250°C at 40°C/s) with a Turbomatrix TD desorber (Perkin-Elmer, USA), and were analyzed with a gas chromatograph coupled with a mass spectrometer and a flame-ionization detector (GC-FID/MS, Clarus 500, Perkin-Elmer, USA) equipped with a DB-5 ms non-polar capillary column (5% phenyl-methylpolysiloxane; 30 m × 0.25 mm ID × 0.25 μm film thickness). ..

Article Title: Volatiles of Grape Inoculated with Microorganisms: Modulation of Grapevine Moth Oviposition and Field Attraction
Article Snippet: .. After a collection time of 60 min, volatiles collected on the fiber were desorbed and injected in a gas-chromatograph coupled to a mass spectrometer (GC-MS, Clarus 500, Perkin Elmer, Waltham, USA) equipped with an Innowax column (30 m × 0.32 mm × 0.5 μm, Agilent, Palo Alto, USA). .. The SPME fiber was desorbed in splitless mode for 5 min in the GC injector port at 250 °C.

Gas Chromatography-Mass Spectrometry:

Article Title: Trigonella foenum (Fenugreek) Induced Apoptosis in Hepatocellular Carcinoma Cell Line, HepG2, Mediated by Upregulation of p53 and Proliferating Cell Nuclear Antigen
Article Snippet: .. GC-MS Analysis The GC-MS analysis was carried out using a Clarus 500 PerkinElmer (Auto system XL) Gas Chromatograph equipped and coupled to a mass detector Turbo Mass Gold, PerkinElmer Turbo Mass 5.1 spectrometer with an Elite-1 (100% dimethylpolysiloxane), 30 m × 0.25 mm ID × 1 μ m of capillary column. .. The instrument was set to an initial temperature of 110°C and maintained at this temperature for 2 min. At the end of this period, the oven temperature was rose up to 280°C, at the rate of an increase of 5°C/min, and maintained for 9 min. Injection port temperature was ensured as 250°C and helium flow rate as one mL/min.

Article Title: Volatiles of Grape Inoculated with Microorganisms: Modulation of Grapevine Moth Oviposition and Field Attraction
Article Snippet: .. After a collection time of 60 min, volatiles collected on the fiber were desorbed and injected in a gas-chromatograph coupled to a mass spectrometer (GC-MS, Clarus 500, Perkin Elmer, Waltham, USA) equipped with an Innowax column (30 m × 0.32 mm × 0.5 μm, Agilent, Palo Alto, USA). .. The SPME fiber was desorbed in splitless mode for 5 min in the GC injector port at 250 °C.

Flow Cytometry:

Article Title: Kinetic Parameters of Denitrification in a River Continuum †
Article Snippet: .. This was done by using a gas chromatograph equipped with 63 Ni electron capture detector (Perkin-Elmer 2000; detector temperature 350°C; flow of 20 ml min−1 ). .. The N2 O concentration was determined by using a standard curve generated with purified N2 O (BOC, Ltd.).

Injection:

Article Title: Volatiles of Grape Inoculated with Microorganisms: Modulation of Grapevine Moth Oviposition and Field Attraction
Article Snippet: .. After a collection time of 60 min, volatiles collected on the fiber were desorbed and injected in a gas-chromatograph coupled to a mass spectrometer (GC-MS, Clarus 500, Perkin Elmer, Waltham, USA) equipped with an Innowax column (30 m × 0.32 mm × 0.5 μm, Agilent, Palo Alto, USA). .. The SPME fiber was desorbed in splitless mode for 5 min in the GC injector port at 250 °C.

Spectrophotometry:

Article Title: Mathematical optimization of the green extraction of polyphenols from grape peels through a cyclic pressurization process
Article Snippet: .. 2.2 Instrumentation and chemicals The instruments used in this study included a UV-VIS spectrophotometer, model 1601, equipped with a 1-cm optical path cuvette (Shimadzu, Tokyo, Japan); a gas chromatograph equipped with a programmable split-splitless injector (PSS), a flame ionization detector (FID) (Auto System XL) (Perkin Elmer, Norwalk, CT, USA) and an Rtx-5 capillary column (l = 30 m, i.d. ..

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  • 92
    PerkinElmer l u 14 c threonine
    Incorporation of radio-labelled or  13 C-labelled carbon sources into lipids of PCF trypanosomes. In panels A and B, [ 14 C]-labelled fatty acid methyl esters and sterols were separated by HPTLC and analyzed as described in the Materials and methods section. Prior to lipid extraction, the EATRO1125.T7T PCF were incubated 16 h in SDM79 containing 4 mM glucose, 4 mM threonine, 4 mM proline, 4 mM leucine, 1 mM isoleucine, 1 mM valine, 4 mM acetate and one radio-labelled tracer (A, [1- 14 C]-acetate; T, L-[U- 14 C]-threonine; G, D-[U- 14 C]-glucose; P, L-[U- 14 C]-proline; L, L-[U- 14 C]-leucine; I, L-[U- 14 C]-isoleucine; V, L-[U- 14 C]-valine). The cell has also been incubated with L-[U- 14 C]-proline, 0.15 mM threonine, 1 mM isoleucine and 1 mM valine, without glucose and acetate (P*). The data are expressed as nmol of precursor (radioactive and non-radioactive molecules) incorporated into fatty acids (A) and/or sterols (B) in 10 8  cells per hour. Error bars indicate mean ± SD of 3 biological replicates.  nd : not detectable. In panel C, the EATRO1125.T7T PCF were incubated in the same conditions as described above with non-enriched (control) or uniformly [ 13 C]-enriched proline, leucine, glucose, threonine or acetate, before processing the sample for sterol analysis by GC-MS. The isotope enrichment factor corresponds to the percentage of molecules containing  13 C-enriched carbons. The amount of each of the five sterols identified is similar in the six experimental conditions; cholesta-5,7,24-trienol, 0.5 ± 0.1 μg; prothothecasterol, 2.2 ± 0.6 μg; ergosta-5,7,24(25)-trienol, 0.7 ± 0.1 μg; ergosta-5,7,25(27)-trienol, 2.2 ± 0.4 μg; cholesterol, 7.9 ± 1.7 μg.
    L U 14 C Threonine, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PerkinElmer myotubes
    The genes targeted by Bhlhe40 and VBH135. The genes expression patterns of C2C12- VBH135 and C2C12-Bhlhe40 <t>myotubes</t> and C2C12-shBhlhe40 myoblasts were examined with qRT-PCR. The relative levels of genes involved in peroxisome or mitochondrial biogenesis and functions are shown in (A) and (B) respectively. The results of genes involved in ROS scavenging are shown in (C) . Binding of Bhlhe40 to target gene promoters was examined in C2C12- Bhlhe40 myotubes (Dox (-)) by ChIP assay (D) . PGC-1α was knockdowned in C2C12- VBH135 (E) and - Bhlhe40 (F) myotubes to reveal PGC-1α-dependent gene regulation. * and * *: p
    Myotubes, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PerkinElmer mature srebp 1 protein forms
    The <t>SREBP-1</t> protein is specifically modified by oleate. SREBP-1 was immunoprecipitated from protein extracts of HepG2 cells previously incubated 24 h with [ 3 H]H 2 O, [ 3 H]oleate, or [ 3 H]palmitoleate (palm.). Immunoprecipitates (IP) were submitted to denaturing gel electrophoresis to separate precursor and mature SREBP-1 (pSREBP-1 and mSREBP-1). Incorporated radioactivity was measured in isolated mSREBP-1 protein bands and presented relative to nonradioactive control (Ctrl). WB, Western blot. The histogram presents the means ± SD of 3 independent experiments. *** P
    Mature Srebp 1 Protein Forms, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    PerkinElmer nat ga ccz01099 nat ga ccz01048
    Representative radio-HPLC chromatograms of in vivo urine stability of (a) [ 68 <t>Ga]Ga-CCZ01099</t> ( n = 3) and (b) [ 68 <t>Ga]Ga-CCZ01048</t> ( n = 4) at 1 h p.i. and quality controls for (c) [ 68 Ga]Ga-CCZ01099 and (d) [ 68 Ga]Ga-CCZ01048.
    Nat Ga Ccz01099 Nat Ga Ccz01048, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Incorporation of radio-labelled or  13 C-labelled carbon sources into lipids of PCF trypanosomes. In panels A and B, [ 14 C]-labelled fatty acid methyl esters and sterols were separated by HPTLC and analyzed as described in the Materials and methods section. Prior to lipid extraction, the EATRO1125.T7T PCF were incubated 16 h in SDM79 containing 4 mM glucose, 4 mM threonine, 4 mM proline, 4 mM leucine, 1 mM isoleucine, 1 mM valine, 4 mM acetate and one radio-labelled tracer (A, [1- 14 C]-acetate; T, L-[U- 14 C]-threonine; G, D-[U- 14 C]-glucose; P, L-[U- 14 C]-proline; L, L-[U- 14 C]-leucine; I, L-[U- 14 C]-isoleucine; V, L-[U- 14 C]-valine). The cell has also been incubated with L-[U- 14 C]-proline, 0.15 mM threonine, 1 mM isoleucine and 1 mM valine, without glucose and acetate (P*). The data are expressed as nmol of precursor (radioactive and non-radioactive molecules) incorporated into fatty acids (A) and/or sterols (B) in 10 8  cells per hour. Error bars indicate mean ± SD of 3 biological replicates.  nd : not detectable. In panel C, the EATRO1125.T7T PCF were incubated in the same conditions as described above with non-enriched (control) or uniformly [ 13 C]-enriched proline, leucine, glucose, threonine or acetate, before processing the sample for sterol analysis by GC-MS. The isotope enrichment factor corresponds to the percentage of molecules containing  13 C-enriched carbons. The amount of each of the five sterols identified is similar in the six experimental conditions; cholesta-5,7,24-trienol, 0.5 ± 0.1 μg; prothothecasterol, 2.2 ± 0.6 μg; ergosta-5,7,24(25)-trienol, 0.7 ± 0.1 μg; ergosta-5,7,25(27)-trienol, 2.2 ± 0.4 μg; cholesterol, 7.9 ± 1.7 μg.

    Journal: PLoS Pathogens

    Article Title: De novo biosynthesis of sterols and fatty acids in the Trypanosoma brucei procyclic form: Carbon source preferences and metabolic flux redistributions

    doi: 10.1371/journal.ppat.1007116

    Figure Lengend Snippet: Incorporation of radio-labelled or 13 C-labelled carbon sources into lipids of PCF trypanosomes. In panels A and B, [ 14 C]-labelled fatty acid methyl esters and sterols were separated by HPTLC and analyzed as described in the Materials and methods section. Prior to lipid extraction, the EATRO1125.T7T PCF were incubated 16 h in SDM79 containing 4 mM glucose, 4 mM threonine, 4 mM proline, 4 mM leucine, 1 mM isoleucine, 1 mM valine, 4 mM acetate and one radio-labelled tracer (A, [1- 14 C]-acetate; T, L-[U- 14 C]-threonine; G, D-[U- 14 C]-glucose; P, L-[U- 14 C]-proline; L, L-[U- 14 C]-leucine; I, L-[U- 14 C]-isoleucine; V, L-[U- 14 C]-valine). The cell has also been incubated with L-[U- 14 C]-proline, 0.15 mM threonine, 1 mM isoleucine and 1 mM valine, without glucose and acetate (P*). The data are expressed as nmol of precursor (radioactive and non-radioactive molecules) incorporated into fatty acids (A) and/or sterols (B) in 10 8 cells per hour. Error bars indicate mean ± SD of 3 biological replicates. nd : not detectable. In panel C, the EATRO1125.T7T PCF were incubated in the same conditions as described above with non-enriched (control) or uniformly [ 13 C]-enriched proline, leucine, glucose, threonine or acetate, before processing the sample for sterol analysis by GC-MS. The isotope enrichment factor corresponds to the percentage of molecules containing 13 C-enriched carbons. The amount of each of the five sterols identified is similar in the six experimental conditions; cholesta-5,7,24-trienol, 0.5 ± 0.1 μg; prothothecasterol, 2.2 ± 0.6 μg; ergosta-5,7,24(25)-trienol, 0.7 ± 0.1 μg; ergosta-5,7,25(27)-trienol, 2.2 ± 0.4 μg; cholesterol, 7.9 ± 1.7 μg.

    Article Snippet: To analyse the carbon source preference , 108 cells in the late exponential phase were incubated for 16 h in 5 ml of modified SDM79 medium containing 4 mM of each carbon source (acetate, glucose, threonine, leucine, proline; except for isoleucine and valine, 1mM) and one radio-labelled source (10 μCi of [1-14 C]-acetate (55.3 mCi/mmol, Perkin-Elmer, Ref NEC084), 5 μCi of D-[U-14 C]-glucose (300 mCi/mmol, Perkin-Elmer, Ref NEC042), 2.5 μCi of L-[U-14 C]-threonine (175 mCi/mmol, American Radiolabeled Chemicals, Ref ARC0677), 2 μCi of L-[U-14 C]-leucine (328 mCi/mmol, Perkin-Elmer, Ref NEC279), 9 μCi of L-[U-14 C]-proline (271 mCi/mmol, Perkin-Elmer, Ref NEC285), 1 μCi of L-[U-14 C]-isoleucine (329 mCi/mmol, Perkin-Elmer, Ref NEC278) or 1 μCi of L-[U-14 C]-valine (271 mCi/mmol, Perkin-Elmer, Ref NEC291).

    Techniques: High Performance Thin Layer Chromatography, Incubation, Gas Chromatography-Mass Spectrometry

    Sterol biosynthesis from glucose, threonine and acetate requires SCP2-thiolase, and fatty acid biosynthesis from acetate requires ASCT. (A) Western blot analyses of the parental (WT), knock-out and tetracycline-induced (.i) or non-induced (.ni) mutant cell lines with the immune sera indicated in the right margin. (B) The top and lower parts represent the relative incorporation of radio-labelled carbon sources (D-[U- 14 C]-glucose, L-[U- 14 C]-threonine, [1- 14 C]-acetate and L-[U- 14 C]-leucine) into fatty acids and sterols, respectively, of tetracycline-induced (.i) and non-induced (.ni)  RNAi SCP2, Δ ach  and Δ asct  cell lines, compared to the parental cell line (PCL). [ 14 C]-labelled fatty acid methyl esters and sterols were separated by HPTLC and analyzed as described in the Materials and methods section. Data are normalized with the parental cell (PCL) values with an arbitrary value of 100 for the PCL samples, which is represented by a horizontal dashed lane. Error bars indicate mean ± SD of 3 biological replicates.  nd : not detectable.

    Journal: PLoS Pathogens

    Article Title: De novo biosynthesis of sterols and fatty acids in the Trypanosoma brucei procyclic form: Carbon source preferences and metabolic flux redistributions

    doi: 10.1371/journal.ppat.1007116

    Figure Lengend Snippet: Sterol biosynthesis from glucose, threonine and acetate requires SCP2-thiolase, and fatty acid biosynthesis from acetate requires ASCT. (A) Western blot analyses of the parental (WT), knock-out and tetracycline-induced (.i) or non-induced (.ni) mutant cell lines with the immune sera indicated in the right margin. (B) The top and lower parts represent the relative incorporation of radio-labelled carbon sources (D-[U- 14 C]-glucose, L-[U- 14 C]-threonine, [1- 14 C]-acetate and L-[U- 14 C]-leucine) into fatty acids and sterols, respectively, of tetracycline-induced (.i) and non-induced (.ni) RNAi SCP2, Δ ach and Δ asct cell lines, compared to the parental cell line (PCL). [ 14 C]-labelled fatty acid methyl esters and sterols were separated by HPTLC and analyzed as described in the Materials and methods section. Data are normalized with the parental cell (PCL) values with an arbitrary value of 100 for the PCL samples, which is represented by a horizontal dashed lane. Error bars indicate mean ± SD of 3 biological replicates. nd : not detectable.

    Article Snippet: To analyse the carbon source preference , 108 cells in the late exponential phase were incubated for 16 h in 5 ml of modified SDM79 medium containing 4 mM of each carbon source (acetate, glucose, threonine, leucine, proline; except for isoleucine and valine, 1mM) and one radio-labelled source (10 μCi of [1-14 C]-acetate (55.3 mCi/mmol, Perkin-Elmer, Ref NEC084), 5 μCi of D-[U-14 C]-glucose (300 mCi/mmol, Perkin-Elmer, Ref NEC042), 2.5 μCi of L-[U-14 C]-threonine (175 mCi/mmol, American Radiolabeled Chemicals, Ref ARC0677), 2 μCi of L-[U-14 C]-leucine (328 mCi/mmol, Perkin-Elmer, Ref NEC279), 9 μCi of L-[U-14 C]-proline (271 mCi/mmol, Perkin-Elmer, Ref NEC285), 1 μCi of L-[U-14 C]-isoleucine (329 mCi/mmol, Perkin-Elmer, Ref NEC278) or 1 μCi of L-[U-14 C]-valine (271 mCi/mmol, Perkin-Elmer, Ref NEC291).

    Techniques: Western Blot, Knock-Out, Mutagenesis, High Performance Thin Layer Chromatography

    The genes targeted by Bhlhe40 and VBH135. The genes expression patterns of C2C12- VBH135 and C2C12-Bhlhe40 myotubes and C2C12-shBhlhe40 myoblasts were examined with qRT-PCR. The relative levels of genes involved in peroxisome or mitochondrial biogenesis and functions are shown in (A) and (B) respectively. The results of genes involved in ROS scavenging are shown in (C) . Binding of Bhlhe40 to target gene promoters was examined in C2C12- Bhlhe40 myotubes (Dox (-)) by ChIP assay (D) . PGC-1α was knockdowned in C2C12- VBH135 (E) and - Bhlhe40 (F) myotubes to reveal PGC-1α-dependent gene regulation. * and * *: p

    Journal: Redox Biology

    Article Title: Bhlhe40 differentially regulates the function and number of peroxisomes and mitochondria in myogenic cells

    doi: 10.1016/j.redox.2018.10.009

    Figure Lengend Snippet: The genes targeted by Bhlhe40 and VBH135. The genes expression patterns of C2C12- VBH135 and C2C12-Bhlhe40 myotubes and C2C12-shBhlhe40 myoblasts were examined with qRT-PCR. The relative levels of genes involved in peroxisome or mitochondrial biogenesis and functions are shown in (A) and (B) respectively. The results of genes involved in ROS scavenging are shown in (C) . Binding of Bhlhe40 to target gene promoters was examined in C2C12- Bhlhe40 myotubes (Dox (-)) by ChIP assay (D) . PGC-1α was knockdowned in C2C12- VBH135 (E) and - Bhlhe40 (F) myotubes to reveal PGC-1α-dependent gene regulation. * and * *: p

    Article Snippet: After starvation, the culture medium of myotubes in 6-well plates were replaced with Krebs-Ringer phosphate (KRPH) buffer containing 0.2 μCi 2-deoxy-D-[3 H]-glucose (2-DG; NET328A250UC, Perkin Elmer) with/without 100 nM insulin and incubate at 37 °C for another 2 h. One well was treated with 10 μM cytochalasin B (CB) for inhibiting actin cytoskeleton mediated glucose uptake and thus served as non-specific/negative glucose uptake control.

    Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Chromatin Immunoprecipitation, Pyrolysis Gas Chromatography

    The effects of Bhlhe40 over-expression on differentiation and metabolism. A Tet-off system was established to stably over-express Bhlhe40 and GFP simultaneously in C2C12 cells (C2C12- Bhlhe40 ), in which the expression is shut off in the presence of Doxycycline (Dox, 25 ng/ml). The expression of Bhlhe40 mRNA (left panel) and protein (right panel) in myoblasts is shown in (A) and its effects on myogenic differentiation (B) was represented by the fusion index (nuclei number in myotubes/total nuclei number). The effects on mtDNA levels of CMB and MT cells, and on Mitotracker stain intensity, SDH activity, and ROS levels in myotubes are shown in (C) , (D) , (E) , and (F) , respectively. (G) Images of C2C12- Bhlhe40 myoblasts transfected with KillerRed-PTS1 expression vector and counterstained with DAPI. The distribution (%) of cells with different numbers of peroxisome/cell and the average peroxisome number/cell are shown in (H) and (I), respectively. * and * *: p

    Journal: Redox Biology

    Article Title: Bhlhe40 differentially regulates the function and number of peroxisomes and mitochondria in myogenic cells

    doi: 10.1016/j.redox.2018.10.009

    Figure Lengend Snippet: The effects of Bhlhe40 over-expression on differentiation and metabolism. A Tet-off system was established to stably over-express Bhlhe40 and GFP simultaneously in C2C12 cells (C2C12- Bhlhe40 ), in which the expression is shut off in the presence of Doxycycline (Dox, 25 ng/ml). The expression of Bhlhe40 mRNA (left panel) and protein (right panel) in myoblasts is shown in (A) and its effects on myogenic differentiation (B) was represented by the fusion index (nuclei number in myotubes/total nuclei number). The effects on mtDNA levels of CMB and MT cells, and on Mitotracker stain intensity, SDH activity, and ROS levels in myotubes are shown in (C) , (D) , (E) , and (F) , respectively. (G) Images of C2C12- Bhlhe40 myoblasts transfected with KillerRed-PTS1 expression vector and counterstained with DAPI. The distribution (%) of cells with different numbers of peroxisome/cell and the average peroxisome number/cell are shown in (H) and (I), respectively. * and * *: p

    Article Snippet: After starvation, the culture medium of myotubes in 6-well plates were replaced with Krebs-Ringer phosphate (KRPH) buffer containing 0.2 μCi 2-deoxy-D-[3 H]-glucose (2-DG; NET328A250UC, Perkin Elmer) with/without 100 nM insulin and incubate at 37 °C for another 2 h. One well was treated with 10 μM cytochalasin B (CB) for inhibiting actin cytoskeleton mediated glucose uptake and thus served as non-specific/negative glucose uptake control.

    Techniques: Over Expression, Stable Transfection, Expressing, Staining, Activity Assay, Transfection, Plasmid Preparation

    The effects of VBH135 over-expression on oxidative metabolism. (A) Catalase activity is enhanced in C2C12-VBH135 cells. Catalase activity in C2C12-VBH135 myotubes was examined in the presence/absence of 20 mM 3-amino-1, 2, 4-triazole (3AT, a catalase inhibitor) and H 2 O 2 (10 mM) for 5–20 min by determining the residual H 2 O 2 amount. The O 2 consumption rate of CMB myoblast (B) and the oxidation of palmitic (C) and oleic acids (D) of cells at CMB and MT stages are determined. The basal and insulin (100 nM) stimulated glucose uptake of these cells at CMB stage is shown in (E) , and their relative insulin responses (insulin stimulated/basal) are shown in (F) . * and * *: p

    Journal: Redox Biology

    Article Title: Bhlhe40 differentially regulates the function and number of peroxisomes and mitochondria in myogenic cells

    doi: 10.1016/j.redox.2018.10.009

    Figure Lengend Snippet: The effects of VBH135 over-expression on oxidative metabolism. (A) Catalase activity is enhanced in C2C12-VBH135 cells. Catalase activity in C2C12-VBH135 myotubes was examined in the presence/absence of 20 mM 3-amino-1, 2, 4-triazole (3AT, a catalase inhibitor) and H 2 O 2 (10 mM) for 5–20 min by determining the residual H 2 O 2 amount. The O 2 consumption rate of CMB myoblast (B) and the oxidation of palmitic (C) and oleic acids (D) of cells at CMB and MT stages are determined. The basal and insulin (100 nM) stimulated glucose uptake of these cells at CMB stage is shown in (E) , and their relative insulin responses (insulin stimulated/basal) are shown in (F) . * and * *: p

    Article Snippet: After starvation, the culture medium of myotubes in 6-well plates were replaced with Krebs-Ringer phosphate (KRPH) buffer containing 0.2 μCi 2-deoxy-D-[3 H]-glucose (2-DG; NET328A250UC, Perkin Elmer) with/without 100 nM insulin and incubate at 37 °C for another 2 h. One well was treated with 10 μM cytochalasin B (CB) for inhibiting actin cytoskeleton mediated glucose uptake and thus served as non-specific/negative glucose uptake control.

    Techniques: Over Expression, Activity Assay

    The SREBP-1 protein is specifically modified by oleate. SREBP-1 was immunoprecipitated from protein extracts of HepG2 cells previously incubated 24 h with [ 3 H]H 2 O, [ 3 H]oleate, or [ 3 H]palmitoleate (palm.). Immunoprecipitates (IP) were submitted to denaturing gel electrophoresis to separate precursor and mature SREBP-1 (pSREBP-1 and mSREBP-1). Incorporated radioactivity was measured in isolated mSREBP-1 protein bands and presented relative to nonradioactive control (Ctrl). WB, Western blot. The histogram presents the means ± SD of 3 independent experiments. *** P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Oleate activates SREBP-1 signaling activity in SCD1-deficient hepatocytes

    doi: 10.1152/ajpendo.00151.2017

    Figure Lengend Snippet: The SREBP-1 protein is specifically modified by oleate. SREBP-1 was immunoprecipitated from protein extracts of HepG2 cells previously incubated 24 h with [ 3 H]H 2 O, [ 3 H]oleate, or [ 3 H]palmitoleate (palm.). Immunoprecipitates (IP) were submitted to denaturing gel electrophoresis to separate precursor and mature SREBP-1 (pSREBP-1 and mSREBP-1). Incorporated radioactivity was measured in isolated mSREBP-1 protein bands and presented relative to nonradioactive control (Ctrl). WB, Western blot. The histogram presents the means ± SD of 3 independent experiments. *** P

    Article Snippet: Bands corresponding to the precursor and mature SREBP-1 protein forms were cut out, and radioactivity was measured in scintillation liquid in a TRI Carb 2800TR liquid scintillation counter (Perkin Elmer).

    Techniques: Modification, Immunoprecipitation, Incubation, Nucleic Acid Electrophoresis, Radioactivity, Isolation, Western Blot

    Oleate specifically increases SREBP-1 expression and nuclear localization. A : SCD1 (red) and SREBP-1 (green) expression was evaluated by immunofluorescence on HepG2 cells transfected with negative control (Ctrl) or SCD1 -targeting siRNA (si SCD1 or siR), or incubated with 1 μM SCD1 inhibitor A939572 (inh.). Cells were treated with 100 μM oleate or palmitoleate (Palm.). DAPI (blue) was used to visualize nuclei. Bars = 20 μm. The SREBP-1 signal per cell ( B and D ), as well as the SREBP-1 nuclear proportion ( C and E ), was measured in all experimental conditions. Each histogram presents the means ± SD of 3 independent experiments. * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Oleate activates SREBP-1 signaling activity in SCD1-deficient hepatocytes

    doi: 10.1152/ajpendo.00151.2017

    Figure Lengend Snippet: Oleate specifically increases SREBP-1 expression and nuclear localization. A : SCD1 (red) and SREBP-1 (green) expression was evaluated by immunofluorescence on HepG2 cells transfected with negative control (Ctrl) or SCD1 -targeting siRNA (si SCD1 or siR), or incubated with 1 μM SCD1 inhibitor A939572 (inh.). Cells were treated with 100 μM oleate or palmitoleate (Palm.). DAPI (blue) was used to visualize nuclei. Bars = 20 μm. The SREBP-1 signal per cell ( B and D ), as well as the SREBP-1 nuclear proportion ( C and E ), was measured in all experimental conditions. Each histogram presents the means ± SD of 3 independent experiments. * P

    Article Snippet: Bands corresponding to the precursor and mature SREBP-1 protein forms were cut out, and radioactivity was measured in scintillation liquid in a TRI Carb 2800TR liquid scintillation counter (Perkin Elmer).

    Techniques: Expressing, Immunofluorescence, Transfection, Negative Control, Incubation

    SREBP-1 expression and de novo lipogenesis are partially restored in the liver of Scd1 knockout mice specifically producing oleate. Livers of control wild-type mice (Ctrl), Scd1 knockout mice (GKO), and mice specifically producing oleate or palmitoleate (GLS5 and GLS3, respectively) were used to evaluate the importance of SCD1 MUFA products in SREBP-1 expression and de novo lipogenesis. A : SREBP-1 expression, for both precursor (pSREBP-1) and mature (mSREBP-1) forms, in total protein extracts was analyzed by Western blotting, in triplicate. Protein levels evaluated by densitometry were normalized against α-tubulin and presented relative to wild-type control. B : expression of key genes implicated in DNL ( Srebp-1 , Acc , and Fas ) were measured by qPCR, normalized against Hprt and presented relative to wild-type control. C : protein expression of pAMPK, AMPK, pACC, and ACC was measured by Western blotting, in triplicate. Protein levels measured by densitometry were normalized against α-tubulin, used to calculate the phosphorylated/total ratio, and presented relative to wild-type control. Each histogram presents the means ± SD of 5 tissue measurements. * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Oleate activates SREBP-1 signaling activity in SCD1-deficient hepatocytes

    doi: 10.1152/ajpendo.00151.2017

    Figure Lengend Snippet: SREBP-1 expression and de novo lipogenesis are partially restored in the liver of Scd1 knockout mice specifically producing oleate. Livers of control wild-type mice (Ctrl), Scd1 knockout mice (GKO), and mice specifically producing oleate or palmitoleate (GLS5 and GLS3, respectively) were used to evaluate the importance of SCD1 MUFA products in SREBP-1 expression and de novo lipogenesis. A : SREBP-1 expression, for both precursor (pSREBP-1) and mature (mSREBP-1) forms, in total protein extracts was analyzed by Western blotting, in triplicate. Protein levels evaluated by densitometry were normalized against α-tubulin and presented relative to wild-type control. B : expression of key genes implicated in DNL ( Srebp-1 , Acc , and Fas ) were measured by qPCR, normalized against Hprt and presented relative to wild-type control. C : protein expression of pAMPK, AMPK, pACC, and ACC was measured by Western blotting, in triplicate. Protein levels measured by densitometry were normalized against α-tubulin, used to calculate the phosphorylated/total ratio, and presented relative to wild-type control. Each histogram presents the means ± SD of 5 tissue measurements. * P

    Article Snippet: Bands corresponding to the precursor and mature SREBP-1 protein forms were cut out, and radioactivity was measured in scintillation liquid in a TRI Carb 2800TR liquid scintillation counter (Perkin Elmer).

    Techniques: Expressing, Knock-Out, Mouse Assay, Western Blot, Real-time Polymerase Chain Reaction

    Stearoyl-CoA desaturase-1 ( SCD1 ) knockdown decreases de novo lipogenesis in HepG2 cells. A : SCD1 protein expression was evaluated by Western blotting in HepG2 cells transfected with negative control (Ctrl) or SCD1 -targeting small interfering (si) RNA (si SCD1 ). Protein levels measured by densitometry were normalized against GAPDH and presented relative to negative control. B : HepG2 cells transfected with negative control (Ctrl) or SCD1 -targeting siRNA (si SCD1 ) were incubated with [ 14 C]acetate. Incorporated radioactivity was measured by thin layer chromatography in total lipids as well as in lipid fractions, including triacylglycerols and diacylglycerols, and presented as counts per minute (CPM) of [ 14 C]lipids per microgram of total protein. C : the expression of proteins involved in lipid metabolism [precursor and mature sterol receptor element binding protein-1 (SREBP-1), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC)] was evaluated by Western blotting in SCD1 -deficient HepG2 cells. Protein levels measured by densitometry were normalized against GAPDH and presented relative to negative control. Each histogram presents the means ± SD of 5 independent experiments. * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Oleate activates SREBP-1 signaling activity in SCD1-deficient hepatocytes

    doi: 10.1152/ajpendo.00151.2017

    Figure Lengend Snippet: Stearoyl-CoA desaturase-1 ( SCD1 ) knockdown decreases de novo lipogenesis in HepG2 cells. A : SCD1 protein expression was evaluated by Western blotting in HepG2 cells transfected with negative control (Ctrl) or SCD1 -targeting small interfering (si) RNA (si SCD1 ). Protein levels measured by densitometry were normalized against GAPDH and presented relative to negative control. B : HepG2 cells transfected with negative control (Ctrl) or SCD1 -targeting siRNA (si SCD1 ) were incubated with [ 14 C]acetate. Incorporated radioactivity was measured by thin layer chromatography in total lipids as well as in lipid fractions, including triacylglycerols and diacylglycerols, and presented as counts per minute (CPM) of [ 14 C]lipids per microgram of total protein. C : the expression of proteins involved in lipid metabolism [precursor and mature sterol receptor element binding protein-1 (SREBP-1), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC)] was evaluated by Western blotting in SCD1 -deficient HepG2 cells. Protein levels measured by densitometry were normalized against GAPDH and presented relative to negative control. Each histogram presents the means ± SD of 5 independent experiments. * P

    Article Snippet: Bands corresponding to the precursor and mature SREBP-1 protein forms were cut out, and radioactivity was measured in scintillation liquid in a TRI Carb 2800TR liquid scintillation counter (Perkin Elmer).

    Techniques: Expressing, Western Blot, Transfection, Negative Control, Incubation, Radioactivity, Thin Layer Chromatography, Binding Assay

    Oleate treatment reverses SREBP-1 expression and maturation defects in SCD1 -deficient HepG2 cells. HepG2 cells were transfected with negative control (Ctrl) or SCD1 -targeting siRNA (si SCD1 ) and treated (or not) with 100 μM oleate. A : fatty acids were extracted and quantified using GC/MS, including the monounsaturated fatty acids (MUFA) palmitoleate and oleate and saturated fatty acids (SFA) palmitate and stearate. The results of 5 experiments are presented as means ± SE. B : SREBP-1 mRNA expression was analyzed by quantitative reverse-transcription-polymerase chain reaction (qPCR) relative to housekeeping gene HPRT1 . C : SREBP-1 protein expression, for both precursor (pSREBP-1) and mature (mSREBP-1) forms, was analyzed by Western blotting. D : protein levels evaluated by densitometry were normalized against GAPDH, used to calculate the mature/total ratio, and presented relative to untreated negative control. Each histogram presents the means ± SE of 5 independent experiments. * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Oleate activates SREBP-1 signaling activity in SCD1-deficient hepatocytes

    doi: 10.1152/ajpendo.00151.2017

    Figure Lengend Snippet: Oleate treatment reverses SREBP-1 expression and maturation defects in SCD1 -deficient HepG2 cells. HepG2 cells were transfected with negative control (Ctrl) or SCD1 -targeting siRNA (si SCD1 ) and treated (or not) with 100 μM oleate. A : fatty acids were extracted and quantified using GC/MS, including the monounsaturated fatty acids (MUFA) palmitoleate and oleate and saturated fatty acids (SFA) palmitate and stearate. The results of 5 experiments are presented as means ± SE. B : SREBP-1 mRNA expression was analyzed by quantitative reverse-transcription-polymerase chain reaction (qPCR) relative to housekeeping gene HPRT1 . C : SREBP-1 protein expression, for both precursor (pSREBP-1) and mature (mSREBP-1) forms, was analyzed by Western blotting. D : protein levels evaluated by densitometry were normalized against GAPDH, used to calculate the mature/total ratio, and presented relative to untreated negative control. Each histogram presents the means ± SE of 5 independent experiments. * P

    Article Snippet: Bands corresponding to the precursor and mature SREBP-1 protein forms were cut out, and radioactivity was measured in scintillation liquid in a TRI Carb 2800TR liquid scintillation counter (Perkin Elmer).

    Techniques: Expressing, Transfection, Negative Control, Gas Chromatography-Mass Spectrometry, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Western Blot

    Representative radio-HPLC chromatograms of in vivo urine stability of (a) [ 68 Ga]Ga-CCZ01099 ( n = 3) and (b) [ 68 Ga]Ga-CCZ01048 ( n = 4) at 1 h p.i. and quality controls for (c) [ 68 Ga]Ga-CCZ01099 and (d) [ 68 Ga]Ga-CCZ01048.

    Journal: ACS Omega

    Article Title: Selective Cyclized α-Melanocyte-Stimulating Hormone Derivative with Multiple N-Methylations for Melanoma Imaging with Positron Emission Tomography

    doi: 10.1021/acsomega.0c00310

    Figure Lengend Snippet: Representative radio-HPLC chromatograms of in vivo urine stability of (a) [ 68 Ga]Ga-CCZ01099 ( n = 3) and (b) [ 68 Ga]Ga-CCZ01048 ( n = 4) at 1 h p.i. and quality controls for (c) [ 68 Ga]Ga-CCZ01099 and (d) [ 68 Ga]Ga-CCZ01048.

    Article Snippet: The membranes were diluted with assay buffer [1:150 dilution, 25 mM HEPES pH 7.0, 1.5 mM CaCl2 , 1 mM MgSO4 , 100 mM NaCl, 0.2% BSA, 1 mM 1,10-phenanthroline, and 1 complete protease inhibitor tablet (EDTA free)/100 mL], incubated with nat Ga-CCZ01099/nat Ga-CCZ01048 and [125 I][Nle4 , D-Phe7 ]-αMSH ([125 I]NDP-αMSH, PerkinElmer) at 37 °C with moderate agitation for 1 h. The binding of [125 I]NDP-αMSH was completed by the nat Ga-CCZ01099/nat Ga-CCZ01048 at increasing concentrations from 0.5 pM to 50 μM.

    Techniques: High Performance Liquid Chromatography, In Vivo

    Representative competitive binding curves and inhibition constant (Ki) values of (a) nat Ga-CCZ01099 and (b) nat Ga-CCZ01048 to hMC1R, hMC3R, hMC4R, and hMC5R. Three or four independent experiments were performed for each condition, as indicated in the figure. NB, no specific binding observed.

    Journal: ACS Omega

    Article Title: Selective Cyclized α-Melanocyte-Stimulating Hormone Derivative with Multiple N-Methylations for Melanoma Imaging with Positron Emission Tomography

    doi: 10.1021/acsomega.0c00310

    Figure Lengend Snippet: Representative competitive binding curves and inhibition constant (Ki) values of (a) nat Ga-CCZ01099 and (b) nat Ga-CCZ01048 to hMC1R, hMC3R, hMC4R, and hMC5R. Three or four independent experiments were performed for each condition, as indicated in the figure. NB, no specific binding observed.

    Article Snippet: The membranes were diluted with assay buffer [1:150 dilution, 25 mM HEPES pH 7.0, 1.5 mM CaCl2 , 1 mM MgSO4 , 100 mM NaCl, 0.2% BSA, 1 mM 1,10-phenanthroline, and 1 complete protease inhibitor tablet (EDTA free)/100 mL], incubated with nat Ga-CCZ01099/nat Ga-CCZ01048 and [125 I][Nle4 , D-Phe7 ]-αMSH ([125 I]NDP-αMSH, PerkinElmer) at 37 °C with moderate agitation for 1 h. The binding of [125 I]NDP-αMSH was completed by the nat Ga-CCZ01099/nat Ga-CCZ01048 at increasing concentrations from 0.5 pM to 50 μM.

    Techniques: Binding Assay, Inhibition