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Hewlett-Packard gas chromatograph
Gas Chromatograph, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 92/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Flow Cytometry:

Article Title: Detoxification of hydrogen sulfide and methanethiol in the cecal mucosa
Article Snippet: .. Samples (0.30 mL) were analyzed for H2 S, CH3 SH, and CH3 SCH3 using a gas chromatograph (model 5890; Hewlett-Packard, Palo Alto, California, USA) equipped with a Teflon column (8 ft × 0.125 in, packed with Chromosil 330 [Supelco], maintained at 80°C with a flow rate of 20 mL/m) and a sulfur chemiluminescence detector (model 355; Sievers Instruments, Boulder, Colorado, USA), which is specific for sulfur-containing gases. .. The identity of the gases was initially verified using gas chromatography/mass spectroscopy, and subsequently by measuring retention times.

other:

Article Title: Losing the Warning Signal: Drought Compromises the Cross-Talk of Signaling Molecules in Quercus ilex Exposed to Ozone
Article Snippet: Separations were performed with a gas chromatograph (HP5890, Hewlett-Packard, Ramsey, MN, United States) equipped with a stainless steel column (150 × 0.4 cm i.d. packed with Hysep T) and a flame ionization detector.

Article Title: Isolation and Screening of Bacteria for Their Diazotrophic Potential and Their Influence on Growth Promotion of Maize Seedlings in Greenhouses
Article Snippet: A gas chromatograph (Hewlett-Packard 5830A) fitted with a 2–2.1 mm, 80–100 mesh, Poropak R column was used and the oven temperature was adjusted to 70°C.

Article Title: Effects of Supplementation of Rumen Protected Fats on Rumen Ecology and Digestibility of Nutrients in Sheep
Article Snippet: Determination of Volatile Fatty Acids The frozen rumen liquor was thawed and centrifuged at 10,000 g at 4 °C for 20 min, and supernatant was collected to determine the concentrations of volatile fatty acids (VFA) The supernatant (0.5 mL) was then mixed with 0.5 mL 20 mM methyl n-valeric acid (internal standard) and was analyzed by gas chromatograph (Hewlett Packard) 6890 GC system (Agilent Technologies, Palo Alto, CA, USA) with bonded phase fused silica capillary column (15m, 0.32 mm ID, 0.25 μm film thickness) equipped with a flame ionization detector (FID).

Article Title: New steady-state microbial community compositions and process performances in biogas reactors induced by temperature disturbances
Article Snippet: The concentrations of acetate, propionate, isobutyrate, butyrate, iso-valerate, and valerate were determined by a gas chromatograph (Hewlett Packard, HP5890 series II) equipped with a flame ionization detector and HP FFAP column (30 m × 0.53 mm × 1.0 μm).

Article Title: Sphingomonas alaskensis Strain AFO1, an Abundant Oligotrophic Ultramicrobacterium from the North Pacific
Article Snippet: Hydroxy functionalities were converted to trimethylsilyl ethers by reaction with bis(trimethylsilyl)trifluoroacetamide (BSTFA) reagent at 80°C for 24 h. FAME were analyzed using a Hewlett-Packard 5890 II gas chromatograph and 5970A mass-selective detector equipped with a 50-m by 0.22-mm (internal diameter) cross-linked methyl silicone (0.33 μm film thickness) fused-silica capillary column.

Article Title: Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil
Article Snippet: The cellular fatty acids of strains Kopri-42T , Kopri-43, and reference strains were extracted after late log phase grown at 10 °C on R2A using the standard MIDI protocol (Sherlock Microbial Identification System, version 6.0B) and analyzed with a gas chromatograph (6890 Series GC System; Hewlett Packard) using the TSBA6 database of the Microbial Identification System .

Mass Spectrometry:

Article Title: Impact of Perinatal Dioxin Exposure on Infant Growth: A Cross-Sectional and Longitudinal Studies in Dioxin-Contaminated Areas in Vietnam
Article Snippet: .. Quantification was performed using a gas chromatograph (HP-6980; Hewlett-Packard, Palo Alto, CA, USA) equipped with a high-resolution mass spectrometer (MStation-JMS700, JEOL, Tokyo, Japan) operating in the selected ion monitoring mode. .. The gas chromatograph was equipped with an ENV-5MS column (Kanto Chemical Co., Inc., Tokyo, Japan).

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    Hewlett-Packard strain mm1ida2h 1
    Biosurfactant production by Cobetia sp. strain <t>MM1IDA2H-1</t> in presence of DBT or microbial competitor. The production of surface-active compounds was evaluated by surface tension measures on cell-free supernatants samples obtained during growth in: (A) minimal media containing DBT as the sole carbon source and; (B) minimal media supplemented with succinate 30 mM and containing inactivated cells of the competitor A. salmonicida . Error bars indicate standard deviation ( n = 3).
    Strain Mm1ida2h 1, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Hewlett-Packard 3αhp
    Examples of MDA-MB-231 tumors and tumor histology from 5αP- and <t>3αHP-treated</t> mice . (A) Large aggressive tumor from a 5αP-treated mouse, with histologic sections showing (B) invasion of rib-cage muscle by the spreading tumor cells, and (C) higher magnification of region with numerous mitoses (arrows). (D) Residual tumor from a 3αHP-treated mouse, with no signs of invasion (E) and showing region of tumor with numerous apoptotic and necrotic cells (F) . Formalin-fixed sections (5 μm) stained with hematoxylin and eosin. Scale bars at 1.0 cm for whole tumors (A, D), at 160 µm for (B) and (E), and at 40 µm for (C) and (F).
    3αhp, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Hewlett-Packard bioactive fractions
    2D confocal imaging: a qualitative analysis. Confocal image stacks of 72-h S. mutans UA 159 biofilms following topical treatment with (a) A. gratissima : fraction Ag 4 ; (b) B. dracunculifolia : fraction Bd 2 ; (c) C. sativum : fraction Cs 4 ; (d) L. sidoides : fraction Ls 3 ; and (e) vehicle (propylene glycol, 6.25% v/v). The structures depicted in red (Dextran, Alexa Fluor 6) represent the extracellular polysaccharides that constitute the biofilm matrix, while the structures depicted in green (Syto 9) are metabolically active bacterial cells (optical magnitude 63x). It can be noted that all <t>bioactive</t> fractions ((a)–(d)) affected the EPS matrix making it less intimately interspersed between and over the cells than did the vehicle alone (e).
    Bioactive Fractions, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Hewlett-Packard disparlure
    Incorporation of D 4 -2me-7-18:Hc into <t>disparlure</t> in isolated pheromone glands. Pheromone glands were incubated with 2.5 μg of D 4 -2me-7-18:Hc in 5 μl of Grace's medium for 3 h; then the glands were extracted and analyzed by SIM GC/MS.
    Disparlure, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biosurfactant production by Cobetia sp. strain MM1IDA2H-1 in presence of DBT or microbial competitor. The production of surface-active compounds was evaluated by surface tension measures on cell-free supernatants samples obtained during growth in: (A) minimal media containing DBT as the sole carbon source and; (B) minimal media supplemented with succinate 30 mM and containing inactivated cells of the competitor A. salmonicida . Error bars indicate standard deviation ( n = 3).

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: Biosurfactant production by Cobetia sp. strain MM1IDA2H-1 in presence of DBT or microbial competitor. The production of surface-active compounds was evaluated by surface tension measures on cell-free supernatants samples obtained during growth in: (A) minimal media containing DBT as the sole carbon source and; (B) minimal media supplemented with succinate 30 mM and containing inactivated cells of the competitor A. salmonicida . Error bars indicate standard deviation ( n = 3).

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Standard Deviation

    Inhibition of quorum-sensing-dependent phenotypes by the biosurfactant produced using the Cobetia sp. strain MM1IDA2H-1.A. Purple phenotype response of Chromobacterium violaceum ATCC 12472 to different concentrations (mg ml −1 ) of biosurfactant (BS) produced by the Cobetia sp. strain MM1IDA2H-1. The production of the purple pigment violacein is under quorum sensing control in C. violaceum , therefore, the loss of this phenotype in the presence of BS was associated with the inhibition of quorum sensing. At the evaluated concentrations no effect on growth was observed.B. Interaction of quorum sensing signals with the biosurfactant. HSLs enriched cell-free supernatants of A. salmonicida were mixed with the biosurfactant and used to induce the quorum sensing pigmented phenotype in the HSL not producer strain CV026. 1: C. violaceum ; 2: CV026 exposed to C. violaceum cell-free supernatant; 3: CV026 exposed to A. salmonicida cell-free supernatant; 4: strain CV026 unexposed; 5: CV026 exposed to A. salmonicida cell-free supernatant mixed with biosurfactant; 6: CV026 exposed to C. violaceum cell-free supernatant mixed with biosurfactant; 7: A. tumefaciens exposed to A. salmonicida cell-free supernatant.C. Surface tension (ST) of water at different concentrations (mg l −1 in logarithmic scale) of the BS, and V. anguillarum biofilm formation at different concentrations (mg l −1 in logarithmic scale). The CMC was established when reduction of ST was stabilized without changes (at 80 mg l −1 ). Values for ST represent the average of three independent assays.

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: Inhibition of quorum-sensing-dependent phenotypes by the biosurfactant produced using the Cobetia sp. strain MM1IDA2H-1.A. Purple phenotype response of Chromobacterium violaceum ATCC 12472 to different concentrations (mg ml −1 ) of biosurfactant (BS) produced by the Cobetia sp. strain MM1IDA2H-1. The production of the purple pigment violacein is under quorum sensing control in C. violaceum , therefore, the loss of this phenotype in the presence of BS was associated with the inhibition of quorum sensing. At the evaluated concentrations no effect on growth was observed.B. Interaction of quorum sensing signals with the biosurfactant. HSLs enriched cell-free supernatants of A. salmonicida were mixed with the biosurfactant and used to induce the quorum sensing pigmented phenotype in the HSL not producer strain CV026. 1: C. violaceum ; 2: CV026 exposed to C. violaceum cell-free supernatant; 3: CV026 exposed to A. salmonicida cell-free supernatant; 4: strain CV026 unexposed; 5: CV026 exposed to A. salmonicida cell-free supernatant mixed with biosurfactant; 6: CV026 exposed to C. violaceum cell-free supernatant mixed with biosurfactant; 7: A. tumefaciens exposed to A. salmonicida cell-free supernatant.C. Surface tension (ST) of water at different concentrations (mg l −1 in logarithmic scale) of the BS, and V. anguillarum biofilm formation at different concentrations (mg l −1 in logarithmic scale). The CMC was established when reduction of ST was stabilized without changes (at 80 mg l −1 ). Values for ST represent the average of three independent assays.

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Inhibition, Produced

    Microscopy of diffusible lipid structures produced by Cobetia sp. strain MM1IDA2H-1. Panels (A) and (C) are respectively TEM and epifluorescence images of cells growing on Bushnell-Hass supplemented with succinate 30 mM. Panels (B) and (D) are respectively TEM and epifluorescence images of cells growing with DBT as the only carbon source. For epifluorescence microscopy Cobetia sp. strain MM1IDA2H-1 cells were stained with BODIPY 505/515 and SYTO 9 to detect respectively lipidic structures and (nucleic acid) cells (red bar = 10 μm).

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: Microscopy of diffusible lipid structures produced by Cobetia sp. strain MM1IDA2H-1. Panels (A) and (C) are respectively TEM and epifluorescence images of cells growing on Bushnell-Hass supplemented with succinate 30 mM. Panels (B) and (D) are respectively TEM and epifluorescence images of cells growing with DBT as the only carbon source. For epifluorescence microscopy Cobetia sp. strain MM1IDA2H-1 cells were stained with BODIPY 505/515 and SYTO 9 to detect respectively lipidic structures and (nucleic acid) cells (red bar = 10 μm).

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Microscopy, Produced, Transmission Electron Microscopy, Epifluorescence Microscopy, Staining

    A. Expression of selected genes of virulence factors of A. salmonicida exposed to biosurfactant. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures (grown to an optical density at 600 nm = 1.0) of A. salmonicida exposed to 80 mg l −1 of biosurfactant (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida not exposed to biosurfactant (NE). In both, the experimental and control conditions, data were normalized to specific 16S RNA-gene of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.B. Expression of selected genes encoding virulence factors of A. salmonicida exposed to Cobetia sp. strain MM1IDA2H-1. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures of A. salmonicida grown with the Cobetia sp. MM1IDA2H-1 strain (optical density at 600 nm = 1.0) (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida (NE). Data from experimental and control conditions were normalized to specific 16S RNA genes of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.

    Journal: Microbial Biotechnology

    Article Title: The hydrocarbon-degrading marine bacterium Cobetia sp. strain MM1IDA2H-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking

    doi: 10.1111/1751-7915.12016

    Figure Lengend Snippet: A. Expression of selected genes of virulence factors of A. salmonicida exposed to biosurfactant. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures (grown to an optical density at 600 nm = 1.0) of A. salmonicida exposed to 80 mg l −1 of biosurfactant (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida not exposed to biosurfactant (NE). In both, the experimental and control conditions, data were normalized to specific 16S RNA-gene of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.B. Expression of selected genes encoding virulence factors of A. salmonicida exposed to Cobetia sp. strain MM1IDA2H-1. Transcript levels of genes were measured by RT-PCR using RNA obtained from cultures of A. salmonicida grown with the Cobetia sp. MM1IDA2H-1 strain (optical density at 600 nm = 1.0) (E). The control condition was the transcript levels of genes measured by RT-PCR using RNA obtained from cultures of A. salmonicida (NE). Data from experimental and control conditions were normalized to specific 16S RNA genes of A. salmonicida . Data (bars are the standard deviation) are representative for three independent biological experiments.

    Article Snippet: Comparison the mass spectrums of Wiley 138 library with the GC-MS chromatograms of biosurfactant produced by Cobetia sp. strain MM1IDA2H-1, treated with Chloroform/Metanol/water in a 2:2:1 ratio, obtained in a Hewlett-Packard 5890 series II gas chromatograph.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Examples of MDA-MB-231 tumors and tumor histology from 5αP- and 3αHP-treated mice . (A) Large aggressive tumor from a 5αP-treated mouse, with histologic sections showing (B) invasion of rib-cage muscle by the spreading tumor cells, and (C) higher magnification of region with numerous mitoses (arrows). (D) Residual tumor from a 3αHP-treated mouse, with no signs of invasion (E) and showing region of tumor with numerous apoptotic and necrotic cells (F) . Formalin-fixed sections (5 μm) stained with hematoxylin and eosin. Scale bars at 1.0 cm for whole tumors (A, D), at 160 µm for (B) and (E), and at 40 µm for (C) and (F).

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Examples of MDA-MB-231 tumors and tumor histology from 5αP- and 3αHP-treated mice . (A) Large aggressive tumor from a 5αP-treated mouse, with histologic sections showing (B) invasion of rib-cage muscle by the spreading tumor cells, and (C) higher magnification of region with numerous mitoses (arrows). (D) Residual tumor from a 3αHP-treated mouse, with no signs of invasion (E) and showing region of tumor with numerous apoptotic and necrotic cells (F) . Formalin-fixed sections (5 μm) stained with hematoxylin and eosin. Scale bars at 1.0 cm for whole tumors (A, D), at 160 µm for (B) and (E), and at 40 µm for (C) and (F).

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Multiple Displacement Amplification, Mouse Assay, Staining

    Summary of the opposing autocrine/paracrine effects of the progesterone metabolites, 5αP and 3αHP, in a stylized ER/PR-negative human breast cell . Evidence presented here shows that a high concentration of 5αP relative to 3αHP, in the microenvironment, promotes initiation and growth of ER/PR-negative human breast cell tumors, whereas a higher concentration of 3αHP, relative to 5αP, suppresses tumorigenesis and promotes normalcy. Progesterone is converted to 3αHP and 5αP in breast cells. Tumorigenic and tumor cells convert more progesterone to 5αP and less to 3αHP than do normal cells. The steroids, being lipophylic, are able to pass out of cells and result in a concentration buildup in the microenvironment. The result is a significant increase in the 5αP-to-3αHP concentration ratio in the microenvironment of tumorigenic cells and within tumorous tissues in comparison with normal (nontumorous) breasts. 3αHP and 5αP bind to specific receptors on the plasma membrane linked to signaling pathways involving PKC, phospholipase C, and Ca 2+ mobilization (3αHP) and MAPK/Erk1/2 (5αP) and to modulators of gene expression. The cancer-inhibiting actions of 3αHP result in decreased proliferation and detachment of cells, increased apoptosis, and suppression of tumor initiation and growth. The cancer-promoting actions of 5αP have the opposite effects and result in stimulation of tumorigenesis and tumor growth. The evidence suggests that high concentrations of 5αP relative to 3αHP in the microenvironment will promote progression toward neoplasia and tumorigenesis, whereas a low 5αP-to-3αHP concentration ratio favors maintenance of the normal state.

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Summary of the opposing autocrine/paracrine effects of the progesterone metabolites, 5αP and 3αHP, in a stylized ER/PR-negative human breast cell . Evidence presented here shows that a high concentration of 5αP relative to 3αHP, in the microenvironment, promotes initiation and growth of ER/PR-negative human breast cell tumors, whereas a higher concentration of 3αHP, relative to 5αP, suppresses tumorigenesis and promotes normalcy. Progesterone is converted to 3αHP and 5αP in breast cells. Tumorigenic and tumor cells convert more progesterone to 5αP and less to 3αHP than do normal cells. The steroids, being lipophylic, are able to pass out of cells and result in a concentration buildup in the microenvironment. The result is a significant increase in the 5αP-to-3αHP concentration ratio in the microenvironment of tumorigenic cells and within tumorous tissues in comparison with normal (nontumorous) breasts. 3αHP and 5αP bind to specific receptors on the plasma membrane linked to signaling pathways involving PKC, phospholipase C, and Ca 2+ mobilization (3αHP) and MAPK/Erk1/2 (5αP) and to modulators of gene expression. The cancer-inhibiting actions of 3αHP result in decreased proliferation and detachment of cells, increased apoptosis, and suppression of tumor initiation and growth. The cancer-promoting actions of 5αP have the opposite effects and result in stimulation of tumorigenesis and tumor growth. The evidence suggests that high concentrations of 5αP relative to 3αHP in the microenvironment will promote progression toward neoplasia and tumorigenesis, whereas a low 5αP-to-3αHP concentration ratio favors maintenance of the normal state.

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Concentration Assay, Expressing

    Hormone levels in tumors . (A) Effect of hormone treatment on 5αP and 3αHP levels in tumors. Hormone levels in tumors from four vehicle-injected, five 5αP-treated, and four 3αHP-treated mice were determined with RIA, as described in Methods. Hormone levels are presented as nanograms per milliliter 5αP and 3αHP and (B) as the ratio of 5αP to 3αHP. (** P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Hormone levels in tumors . (A) Effect of hormone treatment on 5αP and 3αHP levels in tumors. Hormone levels in tumors from four vehicle-injected, five 5αP-treated, and four 3αHP-treated mice were determined with RIA, as described in Methods. Hormone levels are presented as nanograms per milliliter 5αP and 3αHP and (B) as the ratio of 5αP to 3αHP. (** P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Injection, Mouse Assay

    Opposing in vitro effects of 5αP and 3αHP on proliferation of MDA-MB-231 cells used in the in vivo (xenograft) studies . Cells were seeded at 4 × 10 4 cells per dish, allowed to attach for 24 hours, and then treated for 72 hours without (C, control) or with 10 -6 M 5αP and/or 3αHP, and proliferation was determined by cell counts. Data are presented as cell number (mean and SEM; n = 4). ** P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Opposing in vitro effects of 5αP and 3αHP on proliferation of MDA-MB-231 cells used in the in vivo (xenograft) studies . Cells were seeded at 4 × 10 4 cells per dish, allowed to attach for 24 hours, and then treated for 72 hours without (C, control) or with 10 -6 M 5αP and/or 3αHP, and proliferation was determined by cell counts. Data are presented as cell number (mean and SEM; n = 4). ** P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: In Vitro, Multiple Displacement Amplification, In Vivo

    ER/PR-negative breast cell tumor induction and growth are regulated by 5αP and 3αHP . (A) Tumor induction and growth are stimulated by 5αP. MDA-MB-231 cells were implanted in mammary fat pads of 11 mice (day 0, inset); 3 days before (day -3), five mice were injected with vehicle (control; black open circles), and six were injected with 5αP (red, solid circles). Data points represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), and the experiment was terminated on day 40. *Significantly different from controls at P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: ER/PR-negative breast cell tumor induction and growth are regulated by 5αP and 3αHP . (A) Tumor induction and growth are stimulated by 5αP. MDA-MB-231 cells were implanted in mammary fat pads of 11 mice (day 0, inset); 3 days before (day -3), five mice were injected with vehicle (control; black open circles), and six were injected with 5αP (red, solid circles). Data points represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), and the experiment was terminated on day 40. *Significantly different from controls at P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Multiple Displacement Amplification, Mouse Assay, Injection

    3αHP results in suppression and regression of 5αP-induced ER/PR-negative tumors . (A) 3αHP suppresses ER/PR-negative breast cell tumorigenesis in 5αP-pretreated mice. Fourteen mice were treated with 5αP on day -3 and day 11 and then were divided into two groups. One group (Group I) continued to be treated with 5αP, whereas the other group (Group II) was treated with 3αHP on days 27, 36, and 47 (Inset). One mouse from each group was excluded from the final analysis, as explained under Results. The data are presented as the percentage of mice with tumors at termination. (B) 3αHP results in regression of 5αP-induced ER/PR-negative breast cell tumors. Twenty-four mice with MDA-MB-231 cell implants received injections of 5αP on days 0, 20, and 61 (inset); on day 75, the 14 mice with approximately similar-sized small palpable tumors (18 to 34 mm 3 ) were divided into two groups, consisting of seven mice each, which received a single injection of either vehicle (veh) or 3αHP, and the experiment was terminated 24 days later. Bars represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), at the start of treatments (day 75, Initial) and at termination (day 99, Final).

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: 3αHP results in suppression and regression of 5αP-induced ER/PR-negative tumors . (A) 3αHP suppresses ER/PR-negative breast cell tumorigenesis in 5αP-pretreated mice. Fourteen mice were treated with 5αP on day -3 and day 11 and then were divided into two groups. One group (Group I) continued to be treated with 5αP, whereas the other group (Group II) was treated with 3αHP on days 27, 36, and 47 (Inset). One mouse from each group was excluded from the final analysis, as explained under Results. The data are presented as the percentage of mice with tumors at termination. (B) 3αHP results in regression of 5αP-induced ER/PR-negative breast cell tumors. Twenty-four mice with MDA-MB-231 cell implants received injections of 5αP on days 0, 20, and 61 (inset); on day 75, the 14 mice with approximately similar-sized small palpable tumors (18 to 34 mm 3 ) were divided into two groups, consisting of seven mice each, which received a single injection of either vehicle (veh) or 3αHP, and the experiment was terminated 24 days later. Bars represent size (mm 3 ; mean ± SEM) of tumors that developed of a total number of mice per treatment (bracketed values), at the start of treatments (day 75, Initial) and at termination (day 99, Final).

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Mouse Assay, Multiple Displacement Amplification, Injection

    Hormone levels in serum . (A) Serum hormone levels after treatment. Serum samples ( n = 4 to 5) were analyzed 15 to 22 days and 42 days after mice received an injection of vehicle (control), 5αP, or 3αHP. Data points represent nanograms per milliliter (mean ± SEM). (** P

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Hormone levels in serum . (A) Serum hormone levels after treatment. Serum samples ( n = 4 to 5) were analyzed 15 to 22 days and 42 days after mice received an injection of vehicle (control), 5αP, or 3αHP. Data points represent nanograms per milliliter (mean ± SEM). (** P

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: Mouse Assay, Injection

    Conversion of progesterone to 3α-dihydroprogesterone (3αHP) and 5α-dihydroprogesterone (5αP) . In vitro studies have shown that both ER/PR-positive and -negative human breast tissues and cell lines are able to convert progesterone to 3αHP and 5αP by the actions of 3α-hydroxysteroid oxidoreductase (3α-HSO) and 5α-reductase, respectively.

    Journal: Breast Cancer Research : BCR

    Article Title: Progesterone metabolites regulate induction, growth, and suppression of estrogen- and progesterone receptor-negative human breast cell tumors

    doi: 10.1186/bcr3422

    Figure Lengend Snippet: Conversion of progesterone to 3α-dihydroprogesterone (3αHP) and 5α-dihydroprogesterone (5αP) . In vitro studies have shown that both ER/PR-positive and -negative human breast tissues and cell lines are able to convert progesterone to 3αHP and 5αP by the actions of 3α-hydroxysteroid oxidoreductase (3α-HSO) and 5α-reductase, respectively.

    Article Snippet: Mass spectrometry For verification of RIA measurements of 5αP, 3αHP, and progesterone, portions of TLC-separated extracts from four tumor tissues were tested with both RIA and GC/MS (Hewlett-Packard GC-Mass Spectrometer, model 5790A/5970A, used in the selected ion mode (SIM) with a DB-1MS 12-m × 0.2 mm × 0.33 μm cross-linked methyl silicone capillary column).

    Techniques: In Vitro

    2D confocal imaging: a qualitative analysis. Confocal image stacks of 72-h S. mutans UA 159 biofilms following topical treatment with (a) A. gratissima : fraction Ag 4 ; (b) B. dracunculifolia : fraction Bd 2 ; (c) C. sativum : fraction Cs 4 ; (d) L. sidoides : fraction Ls 3 ; and (e) vehicle (propylene glycol, 6.25% v/v). The structures depicted in red (Dextran, Alexa Fluor 6) represent the extracellular polysaccharides that constitute the biofilm matrix, while the structures depicted in green (Syto 9) are metabolically active bacterial cells (optical magnitude 63x). It can be noted that all bioactive fractions ((a)–(d)) affected the EPS matrix making it less intimately interspersed between and over the cells than did the vehicle alone (e).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis

    doi: 10.1155/2015/871316

    Figure Lengend Snippet: 2D confocal imaging: a qualitative analysis. Confocal image stacks of 72-h S. mutans UA 159 biofilms following topical treatment with (a) A. gratissima : fraction Ag 4 ; (b) B. dracunculifolia : fraction Bd 2 ; (c) C. sativum : fraction Cs 4 ; (d) L. sidoides : fraction Ls 3 ; and (e) vehicle (propylene glycol, 6.25% v/v). The structures depicted in red (Dextran, Alexa Fluor 6) represent the extracellular polysaccharides that constitute the biofilm matrix, while the structures depicted in green (Syto 9) are metabolically active bacterial cells (optical magnitude 63x). It can be noted that all bioactive fractions ((a)–(d)) affected the EPS matrix making it less intimately interspersed between and over the cells than did the vehicle alone (e).

    Article Snippet: Phytochemical Analysis of the Essential Oils and Bioactive Fractions by Gas Chromatography Coupled to Mass Spectrometry (GC-MS) Volatile constituents were identified using a Hewlett-Packard 6890 gas chromatograph coupled with an HP-5975 mass selective detector and HP-5 capillary column (30 m × 0.25 mm × 0.25 μ m diameter).

    Techniques: Imaging, Metabolic Labelling

    Incorporation of D 4 -2me-7-18:Hc into disparlure in isolated pheromone glands. Pheromone glands were incubated with 2.5 μg of D 4 -2me-7-18:Hc in 5 μl of Grace's medium for 3 h; then the glands were extracted and analyzed by SIM GC/MS.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Sex pheromone biosynthetic pathway for disparlure in the gypsy moth, Lymantria dispar

    doi: 10.1073/pnas.0236060100

    Figure Lengend Snippet: Incorporation of D 4 -2me-7-18:Hc into disparlure in isolated pheromone glands. Pheromone glands were incubated with 2.5 μg of D 4 -2me-7-18:Hc in 5 μl of Grace's medium for 3 h; then the glands were extracted and analyzed by SIM GC/MS.

    Article Snippet: The stereoisomers of disparlure were separated by chiral HPLC by using a Chiralcel OJ-R (Daicel Chemical, Tokyo) column (2.1 × 150 mm) in a Hewlett–Packard 1090 HPLC coupled to a 1100 mass selective detector.

    Techniques: Isolation, Incubation, Gas Chromatography-Mass Spectrometry

    Proposed biosynthetic pathway for producing the alkene ( A ) and disparlure ( B ) by oenocyte and pheromone gland cells, respectively.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Sex pheromone biosynthetic pathway for disparlure in the gypsy moth, Lymantria dispar

    doi: 10.1073/pnas.0236060100

    Figure Lengend Snippet: Proposed biosynthetic pathway for producing the alkene ( A ) and disparlure ( B ) by oenocyte and pheromone gland cells, respectively.

    Article Snippet: The stereoisomers of disparlure were separated by chiral HPLC by using a Chiralcel OJ-R (Daicel Chemical, Tokyo) column (2.1 × 150 mm) in a Hewlett–Packard 1090 HPLC coupled to a 1100 mass selective detector.

    Techniques:

    Valine incorporation into the alkene 2me-7-18:Hc ( A ) and disparlure ( B ). D 8 -Valine (20 μg per 20 μl of saline) was injected into 1-day-old females once daily until 3 days old (three injections total). One hour after the last injection, hemolymph and pheromone glands were extracted and hydrocarbons were purified and analyzed by GC/MS. MS analysis was performed in the SIM mode, analyzing for specific ions plus 7, because one deuterium is lost during the conversion of valine to isobutyryl-CoA. The ions that were monitored were 43 and 266 for the alkene. These were selected because 266 is the molecular ion and 43 can come from the methyl branch and both are of significant intensity. The ions 43, 141, and 183 were monitored for disparlure. These ions were selected because of their intensity, and the latter two are fragments on either side of the epoxide. Control females were injected with unlabeled valine, and the peaks for ions 50 and 273 were absent in the alkene (data not shown). The labeled ion peaks elute just before the natural unlabeled peaks, which is indicative of deuterium labeling.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Sex pheromone biosynthetic pathway for disparlure in the gypsy moth, Lymantria dispar

    doi: 10.1073/pnas.0236060100

    Figure Lengend Snippet: Valine incorporation into the alkene 2me-7-18:Hc ( A ) and disparlure ( B ). D 8 -Valine (20 μg per 20 μl of saline) was injected into 1-day-old females once daily until 3 days old (three injections total). One hour after the last injection, hemolymph and pheromone glands were extracted and hydrocarbons were purified and analyzed by GC/MS. MS analysis was performed in the SIM mode, analyzing for specific ions plus 7, because one deuterium is lost during the conversion of valine to isobutyryl-CoA. The ions that were monitored were 43 and 266 for the alkene. These were selected because 266 is the molecular ion and 43 can come from the methyl branch and both are of significant intensity. The ions 43, 141, and 183 were monitored for disparlure. These ions were selected because of their intensity, and the latter two are fragments on either side of the epoxide. Control females were injected with unlabeled valine, and the peaks for ions 50 and 273 were absent in the alkene (data not shown). The labeled ion peaks elute just before the natural unlabeled peaks, which is indicative of deuterium labeling.

    Article Snippet: The stereoisomers of disparlure were separated by chiral HPLC by using a Chiralcel OJ-R (Daicel Chemical, Tokyo) column (2.1 × 150 mm) in a Hewlett–Packard 1090 HPLC coupled to a 1100 mass selective detector.

    Techniques: Injection, Purification, Gas Chromatography-Mass Spectrometry, Mass Spectrometry, Labeling

    Incorporation of D 4 -2me-7-18:Hc into disparlure after injection into females. One-day-old females were injected with 5 μg of D 4 -2me-7-18:Hc in 5 μl of Grace's medium once per day for 3 days. Two hours after the last injection, pheromone glands were removed, extracted, and analyzed for incorporation by complete scan GC/MS. ( A ) Ions 141 and 145 were selected from the scan for presentation. ( B ) Complete ion scan under the ion 145 peak. ( C ) Complete ion scan under the ion 141 peak.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Sex pheromone biosynthetic pathway for disparlure in the gypsy moth, Lymantria dispar

    doi: 10.1073/pnas.0236060100

    Figure Lengend Snippet: Incorporation of D 4 -2me-7-18:Hc into disparlure after injection into females. One-day-old females were injected with 5 μg of D 4 -2me-7-18:Hc in 5 μl of Grace's medium once per day for 3 days. Two hours after the last injection, pheromone glands were removed, extracted, and analyzed for incorporation by complete scan GC/MS. ( A ) Ions 141 and 145 were selected from the scan for presentation. ( B ) Complete ion scan under the ion 145 peak. ( C ) Complete ion scan under the ion 141 peak.

    Article Snippet: The stereoisomers of disparlure were separated by chiral HPLC by using a Chiralcel OJ-R (Daicel Chemical, Tokyo) column (2.1 × 150 mm) in a Hewlett–Packard 1090 HPLC coupled to a 1100 mass selective detector.

    Techniques: Injection, Gas Chromatography-Mass Spectrometry

    Partial HPLC/MS chromatograms of racemic mixture of disparlure ( A ), (+)-disparlure ( B ), and disparlure isolated from four pheromone glands ( C ). HPLC/MS conditions are described in Materials and Methods . The chromatograms represent SIM of ion 300 (M + 18).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Sex pheromone biosynthetic pathway for disparlure in the gypsy moth, Lymantria dispar

    doi: 10.1073/pnas.0236060100

    Figure Lengend Snippet: Partial HPLC/MS chromatograms of racemic mixture of disparlure ( A ), (+)-disparlure ( B ), and disparlure isolated from four pheromone glands ( C ). HPLC/MS conditions are described in Materials and Methods . The chromatograms represent SIM of ion 300 (M + 18).

    Article Snippet: The stereoisomers of disparlure were separated by chiral HPLC by using a Chiralcel OJ-R (Daicel Chemical, Tokyo) column (2.1 × 150 mm) in a Hewlett–Packard 1090 HPLC coupled to a 1100 mass selective detector.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Isolation