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    rabbit polyclonal anti gapdh d16h11  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti gapdh
    The expression of lnc_000048 modulates the expression of M1-type macrophage polarization markers. (A) The proportion of CD38-labeled M1 macrophages in the Sh-lnc_000048 group and Sh-NC group was detected by flow cytometry. (B) Representative western blot for <t>interleukin</t> <t>(IL)-1β</t> and tumor necrosis factor-α (TNF-α) in Sh-lnc_000048 and Sh-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase <t>(GAPDH)</t> as a loading control. (C) Representative western blot for IL-1β and TNF-α in Oe-lnc_000048 and Oe-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 (independent samples t -test).
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    Images

    1) Product Images from "Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype"

    Article Title: Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-23-01355

    The expression of lnc_000048 modulates the expression of M1-type macrophage polarization markers. (A) The proportion of CD38-labeled M1 macrophages in the Sh-lnc_000048 group and Sh-NC group was detected by flow cytometry. (B) Representative western blot for interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in Sh-lnc_000048 and Sh-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (C) Representative western blot for IL-1β and TNF-α in Oe-lnc_000048 and Oe-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 (independent samples t -test).
    Figure Legend Snippet: The expression of lnc_000048 modulates the expression of M1-type macrophage polarization markers. (A) The proportion of CD38-labeled M1 macrophages in the Sh-lnc_000048 group and Sh-NC group was detected by flow cytometry. (B) Representative western blot for interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in Sh-lnc_000048 and Sh-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (C) Representative western blot for IL-1β and TNF-α in Oe-lnc_000048 and Oe-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 (independent samples t -test).

    Techniques Used: Expressing, Labeling, Flow Cytometry, Western Blot

    Lnc_000048 promotes M1 macrophage polarization through enhancement of the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated STAT1 (p-STAT1) in M1 macrophages with lnc_000048 knockdown or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot for the total and p-STAT1 in M1 macrophages with lnc_000048 overexpression or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. (C) Representative western blot of the total and p-STAT1 in M1 macrophages treated with JAK1 inhibitor ruxolitinib or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (independent samples t -test), ns: not significant.
    Figure Legend Snippet: Lnc_000048 promotes M1 macrophage polarization through enhancement of the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated STAT1 (p-STAT1) in M1 macrophages with lnc_000048 knockdown or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot for the total and p-STAT1 in M1 macrophages with lnc_000048 overexpression or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. (C) Representative western blot of the total and p-STAT1 in M1 macrophages treated with JAK1 inhibitor ruxolitinib or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (independent samples t -test), ns: not significant.

    Techniques Used: Western Blot, Over Expression

    Lnc_000048 acts upstream of PKR and promotes PKR phosphorylation and activation to enhance the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated PKR in the Sh-lnc_000048 group and Sh-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot of the total and phosphorylated PKR in the Oe-lnc_000048 group and Oe-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (C) Plots of cell viability and half-maximal inhibitory concentrations (IC50) in M1 macrophages treated with various concentrations of the PKR inhibitor C16. (D) The expression of lnc_000048 in M1 macrophages treated with C16 or dimethyl sulfoxide (DMSO) was detected by real-time fluorescence quantitative PCR (qRT-PCR). (E) Representative western blot of the total and phosphorylated PKR, and STAT1 in M1 macrophages treated with C16 or DMSO; protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (F) The mRNA expressions of tumor necrosis factor-α ( TNF-α ), interleukin ( IL-6 ), and IL- β in M1 macrophages treated with C16 or DMSO were detected by qRT-PCR. (G) Representative western blot of the total and phosphorylated STAT1 in C16-treated M1 macrophages with lnc_000048 overexpression or controls, relative protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (H) TNF-α , and IL- β mRNA expression in C16-treated M1 macrophages with lnc_000048 overexpression or controls measured by qRT-PCR. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 (independent samples t -test). ns: Not significant; PKR: double-stranded RNA-dependent protein kinase.
    Figure Legend Snippet: Lnc_000048 acts upstream of PKR and promotes PKR phosphorylation and activation to enhance the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated PKR in the Sh-lnc_000048 group and Sh-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot of the total and phosphorylated PKR in the Oe-lnc_000048 group and Oe-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (C) Plots of cell viability and half-maximal inhibitory concentrations (IC50) in M1 macrophages treated with various concentrations of the PKR inhibitor C16. (D) The expression of lnc_000048 in M1 macrophages treated with C16 or dimethyl sulfoxide (DMSO) was detected by real-time fluorescence quantitative PCR (qRT-PCR). (E) Representative western blot of the total and phosphorylated PKR, and STAT1 in M1 macrophages treated with C16 or DMSO; protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (F) The mRNA expressions of tumor necrosis factor-α ( TNF-α ), interleukin ( IL-6 ), and IL- β in M1 macrophages treated with C16 or DMSO were detected by qRT-PCR. (G) Representative western blot of the total and phosphorylated STAT1 in C16-treated M1 macrophages with lnc_000048 overexpression or controls, relative protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (H) TNF-α , and IL- β mRNA expression in C16-treated M1 macrophages with lnc_000048 overexpression or controls measured by qRT-PCR. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 (independent samples t -test). ns: Not significant; PKR: double-stranded RNA-dependent protein kinase.

    Techniques Used: Activation Assay, Western Blot, Expressing, Fluorescence, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Over Expression

    anti gapdh  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti gapdh
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    Image Search Results


    The expression of lnc_000048 modulates the expression of M1-type macrophage polarization markers. (A) The proportion of CD38-labeled M1 macrophages in the Sh-lnc_000048 group and Sh-NC group was detected by flow cytometry. (B) Representative western blot for interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in Sh-lnc_000048 and Sh-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (C) Representative western blot for IL-1β and TNF-α in Oe-lnc_000048 and Oe-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 (independent samples t -test).

    Journal: Neural Regeneration Research

    Article Title: Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

    doi: 10.4103/NRR.NRR-D-23-01355

    Figure Lengend Snippet: The expression of lnc_000048 modulates the expression of M1-type macrophage polarization markers. (A) The proportion of CD38-labeled M1 macrophages in the Sh-lnc_000048 group and Sh-NC group was detected by flow cytometry. (B) Representative western blot for interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in Sh-lnc_000048 and Sh-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (C) Representative western blot for IL-1β and TNF-α in Oe-lnc_000048 and Oe-NC group M1 macrophages (left), relative IL-1β and TNF-α protein levels were quantified using ImageJ (right), and protein levels were normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 (independent samples t -test).

    Article Snippet: Membranes were incubated with the following primary antibodies and secondary antibodies: anti-tumor necrosis factor-α (TNF-α; 1:2000, Abcam, Cambridge, UK, Cat# ab183218, RRID: AB_2889388), anti-IL-1β (1:1000, Abcam, Cat# ab254360, RRID: AB_2936299), anti-GAPDH (1:3000, Elabscience, Cat# E-AB-40337), Stat1 antibody (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 9172, RRID: AB_2198300), recombination anti-STAT1 (phospho Y701) antibody [EPR3147] (1:1000, Abcam, Cat# ab109457, RRID: AB_10865748), recombination anti-PKR antibody [EPR19374] (1:2000, Abcam, Cat# ab184257, RRID: AB_2916271), recombination anti-PKR (phospho T446) antibody [E120] (1:2000, Abcam, Cat# ab32036, RRID: AB_777310), and peroxidase-labeled rabbit anti-goat IgG (1:10000, Elabscience, Cat# E-AB-1004).

    Techniques: Expressing, Labeling, Flow Cytometry, Western Blot

    Lnc_000048 promotes M1 macrophage polarization through enhancement of the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated STAT1 (p-STAT1) in M1 macrophages with lnc_000048 knockdown or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot for the total and p-STAT1 in M1 macrophages with lnc_000048 overexpression or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. (C) Representative western blot of the total and p-STAT1 in M1 macrophages treated with JAK1 inhibitor ruxolitinib or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (independent samples t -test), ns: not significant.

    Journal: Neural Regeneration Research

    Article Title: Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

    doi: 10.4103/NRR.NRR-D-23-01355

    Figure Lengend Snippet: Lnc_000048 promotes M1 macrophage polarization through enhancement of the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated STAT1 (p-STAT1) in M1 macrophages with lnc_000048 knockdown or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot for the total and p-STAT1 in M1 macrophages with lnc_000048 overexpression or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. (C) Representative western blot of the total and p-STAT1 in M1 macrophages treated with JAK1 inhibitor ruxolitinib or controls; relative p-STAT1 protein levels were quantified using ImageJ, and normalized using GAPDH as a loading control. Data are displayed as mean ± SD ( n = 3). ** P < 0.01, *** P < 0.001 (independent samples t -test), ns: not significant.

    Article Snippet: Membranes were incubated with the following primary antibodies and secondary antibodies: anti-tumor necrosis factor-α (TNF-α; 1:2000, Abcam, Cambridge, UK, Cat# ab183218, RRID: AB_2889388), anti-IL-1β (1:1000, Abcam, Cat# ab254360, RRID: AB_2936299), anti-GAPDH (1:3000, Elabscience, Cat# E-AB-40337), Stat1 antibody (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 9172, RRID: AB_2198300), recombination anti-STAT1 (phospho Y701) antibody [EPR3147] (1:1000, Abcam, Cat# ab109457, RRID: AB_10865748), recombination anti-PKR antibody [EPR19374] (1:2000, Abcam, Cat# ab184257, RRID: AB_2916271), recombination anti-PKR (phospho T446) antibody [E120] (1:2000, Abcam, Cat# ab32036, RRID: AB_777310), and peroxidase-labeled rabbit anti-goat IgG (1:10000, Elabscience, Cat# E-AB-1004).

    Techniques: Western Blot, Over Expression

    Lnc_000048 acts upstream of PKR and promotes PKR phosphorylation and activation to enhance the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated PKR in the Sh-lnc_000048 group and Sh-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot of the total and phosphorylated PKR in the Oe-lnc_000048 group and Oe-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (C) Plots of cell viability and half-maximal inhibitory concentrations (IC50) in M1 macrophages treated with various concentrations of the PKR inhibitor C16. (D) The expression of lnc_000048 in M1 macrophages treated with C16 or dimethyl sulfoxide (DMSO) was detected by real-time fluorescence quantitative PCR (qRT-PCR). (E) Representative western blot of the total and phosphorylated PKR, and STAT1 in M1 macrophages treated with C16 or DMSO; protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (F) The mRNA expressions of tumor necrosis factor-α ( TNF-α ), interleukin ( IL-6 ), and IL- β in M1 macrophages treated with C16 or DMSO were detected by qRT-PCR. (G) Representative western blot of the total and phosphorylated STAT1 in C16-treated M1 macrophages with lnc_000048 overexpression or controls, relative protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (H) TNF-α , and IL- β mRNA expression in C16-treated M1 macrophages with lnc_000048 overexpression or controls measured by qRT-PCR. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 (independent samples t -test). ns: Not significant; PKR: double-stranded RNA-dependent protein kinase.

    Journal: Neural Regeneration Research

    Article Title: Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

    doi: 10.4103/NRR.NRR-D-23-01355

    Figure Lengend Snippet: Lnc_000048 acts upstream of PKR and promotes PKR phosphorylation and activation to enhance the STAT1 signaling pathway. (A) Representative western blot of the total and phosphorylated PKR in the Sh-lnc_000048 group and Sh-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. (B) Representative western blot of the total and phosphorylated PKR in the Oe-lnc_000048 group and Oe-NC group in M1 macrophages; relative phosphorylated PKR protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (C) Plots of cell viability and half-maximal inhibitory concentrations (IC50) in M1 macrophages treated with various concentrations of the PKR inhibitor C16. (D) The expression of lnc_000048 in M1 macrophages treated with C16 or dimethyl sulfoxide (DMSO) was detected by real-time fluorescence quantitative PCR (qRT-PCR). (E) Representative western blot of the total and phosphorylated PKR, and STAT1 in M1 macrophages treated with C16 or DMSO; protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (F) The mRNA expressions of tumor necrosis factor-α ( TNF-α ), interleukin ( IL-6 ), and IL- β in M1 macrophages treated with C16 or DMSO were detected by qRT-PCR. (G) Representative western blot of the total and phosphorylated STAT1 in C16-treated M1 macrophages with lnc_000048 overexpression or controls, relative protein levels were quantified using ImageJ, and protein levels were normalized using GAPDH as a loading control. (H) TNF-α , and IL- β mRNA expression in C16-treated M1 macrophages with lnc_000048 overexpression or controls measured by qRT-PCR. Data are displayed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 (independent samples t -test). ns: Not significant; PKR: double-stranded RNA-dependent protein kinase.

    Article Snippet: Membranes were incubated with the following primary antibodies and secondary antibodies: anti-tumor necrosis factor-α (TNF-α; 1:2000, Abcam, Cambridge, UK, Cat# ab183218, RRID: AB_2889388), anti-IL-1β (1:1000, Abcam, Cat# ab254360, RRID: AB_2936299), anti-GAPDH (1:3000, Elabscience, Cat# E-AB-40337), Stat1 antibody (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 9172, RRID: AB_2198300), recombination anti-STAT1 (phospho Y701) antibody [EPR3147] (1:1000, Abcam, Cat# ab109457, RRID: AB_10865748), recombination anti-PKR antibody [EPR19374] (1:2000, Abcam, Cat# ab184257, RRID: AB_2916271), recombination anti-PKR (phospho T446) antibody [E120] (1:2000, Abcam, Cat# ab32036, RRID: AB_777310), and peroxidase-labeled rabbit anti-goat IgG (1:10000, Elabscience, Cat# E-AB-1004).

    Techniques: Activation Assay, Western Blot, Expressing, Fluorescence, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Over Expression