gap27 peptide  (Bio-Techne corporation)


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    Structured Review

    Bio-Techne corporation gap27 peptide
    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled <t>Gap27</t> peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Gap27 Peptide, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27 peptide/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 peptide - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish"

    Article Title: Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E20-12-0797

    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Figure Legend Snippet: cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Techniques Used: Injection

    gap27 peptide  (Bio-Techne corporation)


    Bioz Verified Symbol Bio-Techne corporation is a verified supplier
    Bioz Manufacturer Symbol Bio-Techne corporation manufactures this product  
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  • 93

    Structured Review

    Bio-Techne corporation gap27 peptide
    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled <t>Gap27</t> peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Gap27 Peptide, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27 peptide/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 peptide - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish"

    Article Title: Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E20-12-0797

    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Figure Legend Snippet: cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Techniques Used: Injection

    gap27 peptide  (Bio-Techne corporation)


    Bioz Verified Symbol Bio-Techne corporation is a verified supplier
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    Structured Review

    Bio-Techne corporation gap27 peptide
    Gap27 Peptide, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27 peptide/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 peptide - by Bioz Stars, 2024-05
    93/100 stars

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    gap27  (Bio-Techne corporation)


    Bioz Verified Symbol Bio-Techne corporation is a verified supplier
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    Structured Review

    Bio-Techne corporation gap27
    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled <t>Gap27</t> peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Gap27, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish"

    Article Title: Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E20-12-0797

    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Figure Legend Snippet: cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Techniques Used: Injection

    gap27  (Bio-Techne corporation)


    Bioz Verified Symbol Bio-Techne corporation is a verified supplier
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    Bio-Techne corporation gap27
    Gap27, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    93/100 stars

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    Bio-Techne corporation gap27 peptide
    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled <t>Gap27</t> peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Gap27 Peptide, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27 peptide/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 peptide - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

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    Bio-Techne corporation gap27
    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled <t>Gap27</t> peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)
    Gap27, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gap27/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gap27 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

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    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Journal: Molecular Biology of the Cell

    Article Title: Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish

    doi: 10.1091/mbc.E20-12-0797

    Figure Lengend Snippet: cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Article Snippet: The well-established GJ-specific mimetic peptide inhibitor, Gap27 ( ; Evans et al. , 2012 ) (Cat. No. 1476, Tocris Bio-Techne Corporation) and a scrambled Gap27 peptide were used. cx43 lh10 embryos were injected with 0.5 mM peptide in phenol red into the pericardium at 24 hpf.

    Techniques: Injection

    cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Journal: Molecular Biology of the Cell

    Article Title: Impaired Cx43 gap junction endocytosis causes morphological and functional defects in zebrafish

    doi: 10.1091/mbc.E20-12-0797

    Figure Lengend Snippet: cx43 lh10 zebrafish exhibit pronounced cardiac defects. (A) Representative images of the heart region of WT and cx43 lh10 3 dpf embryos. cx43 lh10 embryos exhibit malformed, underdeveloped, and elongated hearts, bradycardia, and pericardial edema. (B) Quantification of WT and cx43 lh10 embryos at 2, 3, and 6 dpf indicates a significantly slower heart rate at all time points ( n = 100 for WT and cx43 lh10 embryos at 2, 3, and 6 dpf each, p ≤ 0.05). (C) Percentage of embryos exhibiting profound pericardial edema at 3 dpf ( n = 496 for WT and 468 for cx43 lh10 embryos, p ≤ 0.05). (D) Heart rates of uninjected 2 dpf cx43 lh10 embryos (UN, red), 2 dpf cx43 lh10 embryos injected with scrambled Gap27 peptide (SCRAM, blue), and 2 dpf cx43 lh10 embryos injected with Gap27 peptide inhibitor (green). Note that the heart rate of embryos injected with Gap27 was restored close to WT levels (compare A with D) ( n = 100 [uninfected], 21 [scrambled], 16 [Gap27 injected], p ≤ 0.05; error: SEM). (Also see Supplemental Movies 1 and 2.)

    Article Snippet: The well-established GJ-specific mimetic peptide inhibitor, Gap27 ( ; Evans et al. , 2012 ) (Cat. No. 1476, Tocris Bio-Techne Corporation) and a scrambled Gap27 peptide were used. cx43 lh10 embryos were injected with 0.5 mM peptide in phenol red into the pericardium at 24 hpf.

    Techniques: Injection