Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Delayed Maturation in the Original Galectin-8 Knockout Mouse. Mature, differentiated gastric corpus chief cells from wild-type C57BL/6J mice at 6 weeks of age express ( A ) sulfated mucins (recognized by the antibody Das-1) and ( B ) gastric intrinsic factor. (GIF) in their zymogenic granules. ( C , D ) Expression of sulfated mucins and GIF appear at 3 weeks of age in C57BL/6J mice. ( E , F ). 6 week-old original Lgals8–/– mice express sulfated mucins and GIF in the chief cells, while ( G , H ) chief cells from 3 week-old original Lgals8–/– mice lack Das-1 and anti-GIF reactivity. Scale Bars 100 mm.

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques: Knock-Out, Expressing

Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Quantification of Maturation Defect in the Original Galectin-8 Knockout Mouse. ( A ) Sulfated mucins (orange) and gastric intrinsic factor (black) appear at equivalent ages in the gastric chief cell and serve as equivalent markers of chief cell maturation. The original galectin-8 null mice (purple) exhibit a delay in gastric chief cell maturation as measured by ( B ) Das-1 or ( C ) anti-GIF reactivity in comparison to C57BL/6 mice (black). Data points represent average of at least 200 glands per mouse and horizontal line represents average of three mice. Relative expression of ( D ) pepsinogen C ( Pgc ) and ( E ) gastric intrinsic factor ( Gif ), and ( F ) Mist1 ( Bhlha15 ) in the original Lgals8 –/– line compared to C57BL/6J at 3 and 8 weeks of age. Black data points represent average signal from two reactions for each mouse. Orange bar is average of three mice. Significance calculated with ANOVA and post-hoc T-Test.

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques: Knock-Out, Comparison, Expressing

Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Cell types other than Chief Cells are Largely Unaffected in the Original Galectin-8 null mouse. ( A,B ) Mature pit cells (AAA Lectin), ( C,D ) Neck cells (GSII Lectin), ( E,F ) Parietal cells (ATP4B) are present at 3 weeks of age in both C57BL/6J as well as the original Lgals8 –/– mice at 3 weeks of age.

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques:

Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Single Cell RNA sequencing demonstrates Galectin-8 is not Expressed in Chief Cells. ( A ) UMap of wild-type C57BL/6J at 8 weeks of age labeled by cell type. ( B ) Galectin-8 expressing cells overlaid on UMap. ( C ) DIM plot displaying galectin-8 expression by cell type. Data and UMap from Halstead et al. (2025) .

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques: Single Cell, RNA Sequencing, Labeling, Expressing

Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Bulk RNA sequencing Demonstrates Exceedingly Low Expression of Multimerin 1 in the Original Galectin-8 Knockout Mouse. ( A ) Principal component analysis comparing gene expression in the gastric corpus of C57BL/6J and the Lgals8 –/– mice at 3 and 8 weeks of age demonstrating segregation of mice by genotype and age. ( B ) Lgals8 qRT-PCR confirms knockout of galectin-8 in the gastric corpus of Lgals8 –/– mice. ( C ) qRT-PCR of Mmrn1 confirms dramatic decrease in expression of multimerin 1 in the gastric corpus of Lgals8 –/– mice.

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques: RNA Sequencing, Expressing, Knock-Out, Gene Expression, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Unappreciated Genetic Deletion of Multimerin 1 in the Original Galectin-8 Null Line. shRNA against Lgals8 reduces expression of ( A ) Lgals8 but not ( B ) Mmrn1 in HUVEC cells. ( C ) PCR targeting alpha-synuclein, Multimerin-1 as well as upstream and downstream regions demonstrates genomic deletion of alpha-synuclein and Multimerin-1 in the galectin-8 null mice. ( D ) Sequencing across the deletion reveals an identical deletion to that reported in the C57BL/6J-OlaHsd strain.

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques: shRNA, Expressing, Sequencing

Journal: Scientific Reports

Article Title: Multimerin1 and not Galectin-8 tempers WNT signaling to promote gastric chief cell differentiation

doi: 10.1038/s41598-026-43742-y

Figure Lengend Snippet: Delayed Chief Cell Maturation is a Consequence of Multimerin1 loss, Not Galectin-8. Immunohistochemistry of ( A ) sulfated mucins and ( B ) gastric intrinsic factor in galectin-8 mice is indistinguishable from wild-type C57BL/6J mice. Quantification of the percentage of corpus glands with either ( C ) sulfomucin or ( D ) gastric intrinsic factor expression in galectin-8 null mice compared to wild-type mice. Immunohistochemistry of ( E ) sulfated mucins and ( F ) gastric intrinsic factor in Multimerin-1/alpha-synuclein double knockout mice demonstrates delayed expression relative to wild-type C57BL/6J mice. Quantification of the percentage of corpus glands with either ( G ) sulfomucin or ( H ) gastric intrinsic factor expression in galectin-8 null mice compared to wild-type mice. Data points represent average of at least 200 glands per mouse and horizontal line represents average of three mice. Significance calculated with ANOVA and post-hoc T-test. Scale Bars 100 mm.

Article Snippet: Galectin-8 Triple Knockout , Yehiel Zick , Maintained KOxKO , .

Techniques: Immunohistochemistry, Expressing, Double Knockout