g418 disulfate salt  (Millipore)


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    Structured Review

    Millipore g418 disulfate salt
    G418 Disulfate Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g418 disulfate salt/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    g418 disulfate salt - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
    Article Snippet: .. At the time of 72 h after transduction, infected cells were selected by using 1.0–2.0 μg per ml of puromycin (P8833; Sigma-Aldrich, St Louis, MO) and/or 600–800 μg per ml G418 disulfate salt (A1720, Sigma-Aldrich, St Louis, MO). .. Unless otherwise indicated, for live-cell single-molecule imaging experiments, the fusions were expressed at the basal level without administrating doxycycline.

    Clone Assay:

    Article Title: Abnormal number cell division of human thyroid anaplastic carcinoma cell line, SW 1736
    Article Snippet: The eGFP-H2B plasmid was constructed by cloning of the human H2B DNA into plasmid pEGFP-C1 (Clontech Laboratories, Inc., Mounain View, CA, USA). .. The eGFP-H2B plasmid was transfected i\nto SW1736 human thyroid anaplastic carcinoma cells by electroporation with Gene Pulser II Electroporation System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co., St. Louis, MO, USA).

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. NONO was cloned for protein purification from the pEGFP vector into a pET-16 b (Novagen) vector using the primers shown in Supplementary Table S1 .

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: This plasmid was digested with Kpn I and Hind III, and the restriction fragment, containing murine iNOS promoter and part of exon 1, was then cloned into the Kpn I/Hind III site of firefly luciferase reporter plasmid pGL4.17(luc2/neo) (Promega, Madison, WI, USA) generating pGL4(miNOS-prom)neo, in which the luciferase gene is driven by murine iNOS promoter. .. Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml).

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich). .. The parental and mutant viruses were derived from EHV-1 strain Ab4, which was cloned as an infectious bacterial artificial chromosome (BAC).

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720). .. TET2 expression was confirmed in positive clones by Western blot (WB) using anti-TET2 antibody (1:500, ab1789, Abcam) and anti-β-actin (1:5000, sc-47778, Santa Cruz) as a loading control.

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: .. CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich). .. C3H1OT1/2 murine mesenchymal cells transfected with EWS-FLI1 (C3H1OT1/2 EF) and with CD99 (C3H1OT1/2 EF-CD99) were cultured as described previously .

    Luciferase:

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: This plasmid was digested with Kpn I and Hind III, and the restriction fragment, containing murine iNOS promoter and part of exon 1, was then cloned into the Kpn I/Hind III site of firefly luciferase reporter plasmid pGL4.17(luc2/neo) (Promega, Madison, WI, USA) generating pGL4(miNOS-prom)neo, in which the luciferase gene is driven by murine iNOS promoter. .. Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml).

    Stable Transfection:

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: To reduce the PS1 gene expression we used CHO cells stably over expressing wild-type human PS1 and APP (CHO-PS1/APP) [ ]. .. CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC).

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: .. Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. To evaluate proliferation, colorectal cancer cells stably expressing KRAS4AG12V and KRAS4BG12V , respectively, were seeded at a density of 1,500 cells per well in a 96-well plate.

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: .. The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. The HR reporter construct ‘DR-GFP’ [kindly provided by Dr. Maria Jasin; ( )] was integrated into the genomic DNA of MCF-7 cells by hygromycin selection (400 µg/ml; Invitrogen).

    Article Title: Quantitative Modulation of PpIX Fluorescence and Improved Glioma Visualization
    Article Snippet: The human GBM cell line U87wtEGFR overexpressing the EGFR gene, was generously provided by Prof. Dr. Frank Furnari (Laboratory of Tumor Biology, Ludwig Institute for Cancer Research, University of California-San Diego) and was cultured in DMEM GlutaMAX, 10% FBS and 1% penicillin-streptomycin, supplemented with 100 μg/mL of G418 disulfate salt (A1720, Sigma-Aldrich). .. U87MG vIII 4.12 cells stably expresses high level of the mutant EGFR variant III (deletion of exons 2-7) (CL 01004-CLTH, Tebu-bio) were maintained in DMEM GlutaMAX, 10% FBS, 1% penicillin-streptomycin and 0.2% of gentamicin 10 mg/mL (15710049, Thermo Fisher Scientific, Life Technologies Europe), enriched with 100 μg/mL of G418.

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: To create a stable transfection with murine iNOS promoter reporter plasmid, J774 cells were transfected with pGL4(miNOS-prom)neo using Lipofectamine 2000 (Invitrogen Co.) according to the manufacturer's instructions. .. Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml).

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: ASP14 cell line, a stable cell line generated from A673 EWS cell line transfected with doxycyclin-inducible shRNA targeting EWS-FLI1 ( ) was cultured in complete medium supplemented with 20 μg/ml Blasticidin (Invitrogen, Grand Island, NY, USA) and 50 μg/ml Zeocin (Sigma Aldrich, St Louis, MO, USA). .. CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich).

    Blocking Assay:

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: .. CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC). .. Without removing the cell media, 24 h after the first transfection cells were transfected with the same siRNA (30 nM) and incubated for an additional 18 h.

    Cytotoxicity Assay:

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. At specified time points, an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed according to the manufacturer's instructions (Cell Viability, Proliferation and Cytotoxicity Assay kit; DOGEN, Seoul, Korea).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. Absorbance at 450 nm was measured using a microplate reader (VersaMax ELISA Microplate Reader, Molecular Devices, Sunnyvale, CA, USA).

    Incubation:

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC). .. Without removing the cell media, 24 h after the first transfection cells were transfected with the same siRNA (30 nM) and incubated for an additional 18 h.

    Article Title: Quantitative Modulation of PpIX Fluorescence and Improved Glioma Visualization
    Article Snippet: The human GBM cell line U87wtEGFR overexpressing the EGFR gene, was generously provided by Prof. Dr. Frank Furnari (Laboratory of Tumor Biology, Ludwig Institute for Cancer Research, University of California-San Diego) and was cultured in DMEM GlutaMAX, 10% FBS and 1% penicillin-streptomycin, supplemented with 100 μg/mL of G418 disulfate salt (A1720, Sigma-Aldrich). .. Cells were harvested by incubation for 5 min at 37°C with 500 μL TrypLE™ Express Enzyme (1X), no phenol red (12604021, Gibco® Life technologies Europe) and blocked with DMEM supplemented with 10% FBS.

    Proliferation Assay:

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: Paragraph title: Stable cell lines and cell proliferation assay ... Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells.

    Cell Culture:

    Article Title: Abnormal number cell division of human thyroid anaplastic carcinoma cell line, SW 1736
    Article Snippet: Paragraph title: Cell culture, transfection of the eGFP-H2B plasmid ... The eGFP-H2B plasmid was transfected i\nto SW1736 human thyroid anaplastic carcinoma cells by electroporation with Gene Pulser II Electroporation System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co., St. Louis, MO, USA).

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: Paragraph title: Cell lines, cell culture, and DNA constructs ... The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker.

    Article Title: Quantitative Modulation of PpIX Fluorescence and Improved Glioma Visualization
    Article Snippet: .. The human GBM cell line U87wtEGFR overexpressing the EGFR gene, was generously provided by Prof. Dr. Frank Furnari (Laboratory of Tumor Biology, Ludwig Institute for Cancer Research, University of California-San Diego) and was cultured in DMEM GlutaMAX, 10% FBS and 1% penicillin-streptomycin, supplemented with 100 μg/mL of G418 disulfate salt (A1720, Sigma-Aldrich). .. U87MG vIII 4.12 cells stably expresses high level of the mutant EGFR variant III (deletion of exons 2-7) (CL 01004-CLTH, Tebu-bio) were maintained in DMEM GlutaMAX, 10% FBS, 1% penicillin-streptomycin and 0.2% of gentamicin 10 mg/mL (15710049, Thermo Fisher Scientific, Life Technologies Europe), enriched with 100 μg/mL of G418.

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml). .. After the selection, the survived clones were pooled to give rise to J774-pGL4(miNOS-prom)neo cell line and further cultured in the presence of 400 µg/ml of G418.

    Article Title: Nucleoside Reverse Transcriptase Inhibitors Suppress Laser-Induced Choroidal Neovascularization in Mice
    Article Snippet: Paragraph title: Cell Culture ... HEK293 cells with stable expression of human or mouse P2X7 receptors (HEK293-hP2X7 or HEK293-mP2X7) were maintained as above and with G418 disulfate salt (0.4 mg/mL; Sigma-Aldrich Corp.).

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: TC-CTR-shRNA cells and TC-CD99-shRNA clones were established and cultured as previously described . .. CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich).

    Expressing:

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: To reduce the PS1 gene expression we used CHO cells stably over expressing wild-type human PS1 and APP (CHO-PS1/APP) [ ]. .. CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC).

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: .. Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. To evaluate proliferation, colorectal cancer cells stably expressing KRAS4AG12V and KRAS4BG12V , respectively, were seeded at a density of 1,500 cells per well in a 96-well plate.

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720). .. TET2 expression was checked by qRT-PCR using the specific primers for TET2 transcription variant1 detection ( ).

    Article Title: Nucleoside Reverse Transcriptase Inhibitors Suppress Laser-Induced Choroidal Neovascularization in Mice
    Article Snippet: .. HEK293 cells with stable expression of human or mouse P2X7 receptors (HEK293-hP2X7 or HEK293-mP2X7) were maintained as above and with G418 disulfate salt (0.4 mg/mL; Sigma-Aldrich Corp.). .. Primary human RPE cells were isolated as described previously.

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich). .. TC-71 cells stably expressing miR-34a (TCpMIF miR-34a) were obtained by infection with the feline immunodeficiency lentivirus-based construct pMIF-cGFPZeo-miR-34a (System Biosciences, Mountain View, CA, USA), as previously described .

    Modification:

    Article Title: Quantitative Modulation of PpIX Fluorescence and Improved Glioma Visualization
    Article Snippet: Cell Culture Human GBM cell line U87MG obtained from American Type Culture Collection (89081402-1VL, Sigma-Aldrich) was maintained in Dulbecco's Modified Eagle Medium (DMEM, 61965, Gibco® Life technologies Europe), GlutaMAX cell culture medium supplemented with 10% fetal bovine serum (FBS, 10270, Gibco® Life technologies Europe), 1% non-essential amino acids (MEM NEAA, 11140, Gibco® Life technologies Europe), 1 mM sodium pyruvate (S8636, Sigma-Aldrich), penicillin 10,000 units/mL and streptomycin 10,000 μg/mL (15140, Gibco® Life technologies Europe). .. The human GBM cell line U87wtEGFR overexpressing the EGFR gene, was generously provided by Prof. Dr. Frank Furnari (Laboratory of Tumor Biology, Ludwig Institute for Cancer Research, University of California-San Diego) and was cultured in DMEM GlutaMAX, 10% FBS and 1% penicillin-streptomycin, supplemented with 100 μg/mL of G418 disulfate salt (A1720, Sigma-Aldrich).

    Article Title: Nucleoside Reverse Transcriptase Inhibitors Suppress Laser-Induced Choroidal Neovascularization in Mice
    Article Snippet: Primary human RPE and HEK293 cells were maintained at 37°C in 5% CO2 and were grown in Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin (VWR International, Radnor, PA, USA). .. HEK293 cells with stable expression of human or mouse P2X7 receptors (HEK293-hP2X7 or HEK293-mP2X7) were maintained as above and with G418 disulfate salt (0.4 mg/mL; Sigma-Aldrich Corp.).

    Western Blot:

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720). .. TET2 expression was confirmed in positive clones by Western blot (WB) using anti-TET2 antibody (1:500, ab1789, Abcam) and anti-β-actin (1:5000, sc-47778, Santa Cruz) as a loading control.

    Derivative Assay:

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich). .. The parental and mutant viruses were derived from EHV-1 strain Ab4, which was cloned as an infectious bacterial artificial chromosome (BAC).

    Electroporation:

    Article Title: Abnormal number cell division of human thyroid anaplastic carcinoma cell line, SW 1736
    Article Snippet: .. The eGFP-H2B plasmid was transfected i\nto SW1736 human thyroid anaplastic carcinoma cells by electroporation with Gene Pulser II Electroporation System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co., St. Louis, MO, USA). .. 1.2 Imaging of transfected SW1736 SW 1736 human thyroid anaplastic carcinoma cells Live cell imaging of transfected SW1736 human thyroid anaplastic carcinoma cells was performed as follows.

    Transfection:

    Article Title: Abnormal number cell division of human thyroid anaplastic carcinoma cell line, SW 1736
    Article Snippet: .. The eGFP-H2B plasmid was transfected i\nto SW1736 human thyroid anaplastic carcinoma cells by electroporation with Gene Pulser II Electroporation System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co., St. Louis, MO, USA). .. 1.2 Imaging of transfected SW1736 SW 1736 human thyroid anaplastic carcinoma cells Live cell imaging of transfected SW1736 human thyroid anaplastic carcinoma cells was performed as follows.

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: .. CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC). .. Without removing the cell media, 24 h after the first transfection cells were transfected with the same siRNA (30 nM) and incubated for an additional 18 h.

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: .. Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. To evaluate proliferation, colorectal cancer cells stably expressing KRAS4AG12V and KRAS4BG12V , respectively, were seeded at a density of 1,500 cells per well in a 96-well plate.

    Article Title: Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
    Article Snippet: At the time of 12 h after transfection, the medium was replaced with 10 ml DMEM supplemented with 10% FBS, 2 mM l -glutamine, and 100 units per ml penicillin G sodium. .. At the time of 72 h after transduction, infected cells were selected by using 1.0–2.0 μg per ml of puromycin (P8833; Sigma-Aldrich, St Louis, MO) and/or 600–800 μg per ml G418 disulfate salt (A1720, Sigma-Aldrich, St Louis, MO).

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: .. Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml). .. After the selection, the survived clones were pooled to give rise to J774-pGL4(miNOS-prom)neo cell line and further cultured in the presence of 400 µg/ml of G418.

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: .. The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich). .. The parental and mutant viruses were derived from EHV-1 strain Ab4, which was cloned as an infectious bacterial artificial chromosome (BAC).

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: .. The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720). .. TET2 expression was checked by qRT-PCR using the specific primers for TET2 transcription variant1 detection ( ).

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: ASP14 cell line, a stable cell line generated from A673 EWS cell line transfected with doxycyclin-inducible shRNA targeting EWS-FLI1 ( ) was cultured in complete medium supplemented with 20 μg/ml Blasticidin (Invitrogen, Grand Island, NY, USA) and 50 μg/ml Zeocin (Sigma Aldrich, St Louis, MO, USA). .. CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich).

    Infection:

    Article Title: Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
    Article Snippet: .. At the time of 72 h after transduction, infected cells were selected by using 1.0–2.0 μg per ml of puromycin (P8833; Sigma-Aldrich, St Louis, MO) and/or 600–800 μg per ml G418 disulfate salt (A1720, Sigma-Aldrich, St Louis, MO). .. Unless otherwise indicated, for live-cell single-molecule imaging experiments, the fusions were expressed at the basal level without administrating doxycycline.

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich). .. TC-71 cells stably expressing miR-34a (TCpMIF miR-34a) were obtained by infection with the feline immunodeficiency lentivirus-based construct pMIF-cGFPZeo-miR-34a (System Biosciences, Mountain View, CA, USA), as previously described .

    Mutagenesis:

    Article Title: Quantitative Modulation of PpIX Fluorescence and Improved Glioma Visualization
    Article Snippet: The human GBM cell line U87wtEGFR overexpressing the EGFR gene, was generously provided by Prof. Dr. Frank Furnari (Laboratory of Tumor Biology, Ludwig Institute for Cancer Research, University of California-San Diego) and was cultured in DMEM GlutaMAX, 10% FBS and 1% penicillin-streptomycin, supplemented with 100 μg/mL of G418 disulfate salt (A1720, Sigma-Aldrich). .. U87MG vIII 4.12 cells stably expresses high level of the mutant EGFR variant III (deletion of exons 2-7) (CL 01004-CLTH, Tebu-bio) were maintained in DMEM GlutaMAX, 10% FBS, 1% penicillin-streptomycin and 0.2% of gentamicin 10 mg/mL (15710049, Thermo Fisher Scientific, Life Technologies Europe), enriched with 100 μg/mL of G418.

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich). .. The parental and mutant viruses were derived from EHV-1 strain Ab4, which was cloned as an infectious bacterial artificial chromosome (BAC).

    Generated:

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: RK13 cells that constitutively express either the ER marker calreticulin or the Golgi marker β-1,4-galactosyltransferase fused to enhanced green fluorescent protein (EGFP) were generated by transfection of the plasmids pER-EGFP and pGolgi-EGFP, respectively, which were kindly provided by Michael Veit (Freie Universität Berlin, Berlin, Germany). .. The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich).

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: ASP14 cell line, a stable cell line generated from A673 EWS cell line transfected with doxycyclin-inducible shRNA targeting EWS-FLI1 ( ) was cultured in complete medium supplemented with 20 μg/ml Blasticidin (Invitrogen, Grand Island, NY, USA) and 50 μg/ml Zeocin (Sigma Aldrich, St Louis, MO, USA). .. CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich).

    Non-Homologous End Joining:

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: .. The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. The HR reporter construct ‘DR-GFP’ [kindly provided by Dr. Maria Jasin; ( )] was integrated into the genomic DNA of MCF-7 cells by hygromycin selection (400 µg/ml; Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Plant, Animal, and Fungal Micronutrient Queuosine Is Salvaged by Members of the DUF2419 Protein Family
    Article Snippet: .. The cells were grown on yeast extract peptone dextrose agar supplemented with G418 disulfate salt (Sigma, 200 μg/mL) at 30 °C, and the genotypes were checked by PCR ( ). ..

    Imaging:

    Article Title: Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
    Article Snippet: At the time of 72 h after transduction, infected cells were selected by using 1.0–2.0 μg per ml of puromycin (P8833; Sigma-Aldrich, St Louis, MO) and/or 600–800 μg per ml G418 disulfate salt (A1720, Sigma-Aldrich, St Louis, MO). .. Unless otherwise indicated, for live-cell single-molecule imaging experiments, the fusions were expressed at the basal level without administrating doxycycline.

    MTT Assay:

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. At specified time points, an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed according to the manufacturer's instructions (Cell Viability, Proliferation and Cytotoxicity Assay kit; DOGEN, Seoul, Korea).

    Passaging:

    Article Title: Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody
    Article Snippet: An anti-Her2 monoclonal antibody-producing CHO-K1 cell line (SH87) was cultivated in PFCDM supplemented with 6 g/l D-(+)-Glucose (Sigma-Aldrich), 8 mM L-Glutamine (Sigma-Aldrich), 0.1% Pluronic F-68 (Life Technologies), and 600 µg/ml G418 disulfate salt (Sigma-Aldrich). .. Passaging of CHO-K1 and SH87 cells were performed every 3 to 4 days as reported previously .

    Isolation:

    Article Title: Nucleoside Reverse Transcriptase Inhibitors Suppress Laser-Induced Choroidal Neovascularization in Mice
    Article Snippet: HEK293 cells with stable expression of human or mouse P2X7 receptors (HEK293-hP2X7 or HEK293-mP2X7) were maintained as above and with G418 disulfate salt (0.4 mg/mL; Sigma-Aldrich Corp.). .. Primary human RPE cells were isolated as described previously.

    Negative Control:

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: TET2 transfection LN229 cell line was transfected in order to overexpress TET2 (GeneCopedia, EX-H3630-M43), incorparating a non-effective Scramble construct as negative control (GeneCopedia, EX-EGFP-M43), using Lipofectamine 3000 transfection Kit (Invitrogen, L3000015) as per the manufacturer´s recommendations. .. The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720).

    Purification:

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: .. The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich). .. The parental and mutant viruses were derived from EHV-1 strain Ab4, which was cloned as an infectious bacterial artificial chromosome (BAC).

    Protein Purification:

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. NONO was cloned for protein purification from the pEGFP vector into a pET-16 b (Novagen) vector using the primers shown in Supplementary Table S1 .

    Sequencing:

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: Preparation of stable J774-pGL4(miNOS-prom)neo and HEK293-pNF-κB(luc)neo reporter cell lines The pGL-MNOS II-5'-Luc plasmid [ ] containing 5'-flanking sequence (1,171 bp, positions -1,570 to +141; promoter and a part of exon 1) of the murine iNOS gene was provided by Professor Harmut Kleinert (Johannes Gutenberg University, Mainz, Germany). .. Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml).

    Positron Emission Tomography:

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. NONO was cloned for protein purification from the pEGFP vector into a pET-16 b (Novagen) vector using the primers shown in Supplementary Table S1 .

    Quantitative RT-PCR:

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720). .. TET2 expression was checked by qRT-PCR using the specific primers for TET2 transcription variant1 detection ( ).

    Plasmid Preparation:

    Article Title: Abnormal number cell division of human thyroid anaplastic carcinoma cell line, SW 1736
    Article Snippet: .. The eGFP-H2B plasmid was transfected i\nto SW1736 human thyroid anaplastic carcinoma cells by electroporation with Gene Pulser II Electroporation System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co., St. Louis, MO, USA). .. 1.2 Imaging of transfected SW1736 SW 1736 human thyroid anaplastic carcinoma cells Live cell imaging of transfected SW1736 human thyroid anaplastic carcinoma cells was performed as follows.

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: PS1 over-expressing cells silencing by siRNA CHO cells (400,000 cells/well) were grown in DMEM® (Gibco) containing 10 % Fetal Bovine Serum (Gibco) and 1 % Penicillin/Streptomycin (Sigma-Aldrich), and they were transfected with a construct encoding full-length PS1 (2 μg cDNA) [ ] or with the pcDNA3 expression plasmid alone (Invitrogen), using Lipofectamine 2000® (Invitrogen). .. CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC).

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: To create a stable transfection with murine iNOS promoter reporter plasmid, J774 cells were transfected with pGL4(miNOS-prom)neo using Lipofectamine 2000 (Invitrogen Co.) according to the manufacturer's instructions. .. Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml).

    Functional Assay:

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich). .. Empty lentivirus (pMIF-cGFPZeo EV) was used as a control; cells were selected with 100 μg/ml Zeocin (Sigma Aldrich) before performing functional studies ( ).

    shRNA:

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: ASP14 cell line, a stable cell line generated from A673 EWS cell line transfected with doxycyclin-inducible shRNA targeting EWS-FLI1 ( ) was cultured in complete medium supplemented with 20 μg/ml Blasticidin (Invitrogen, Grand Island, NY, USA) and 50 μg/ml Zeocin (Sigma Aldrich, St Louis, MO, USA). .. CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich).

    Selection:

    Article Title: Comparison of liver oncogenic potential among human RAS isoforms
    Article Snippet: .. Two days post transfection, cells were placed in culture media containing G418 disulfate salt (G-8168; Sigma-Aldrich) for the selection of stably expressing cells. .. To evaluate proliferation, colorectal cancer cells stably expressing KRAS4AG12V and KRAS4BG12V , respectively, were seeded at a density of 1,500 cells per well in a 96-well plate.

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: .. The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. The HR reporter construct ‘DR-GFP’ [kindly provided by Dr. Maria Jasin; ( )] was integrated into the genomic DNA of MCF-7 cells by hygromycin selection (400 µg/ml; Invitrogen).

    Article Title: Anti-inflammatory properties of a dual PPARgamma/alpha agonist muraglitazar in in vitro and in vivo models
    Article Snippet: Transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co.) treatment (800 µg/ml). .. After the selection, the survived clones were pooled to give rise to J774-pGL4(miNOS-prom)neo cell line and further cultured in the presence of 400 µg/ml of G418.

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: .. The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720). .. TET2 expression was checked by qRT-PCR using the specific primers for TET2 transcription variant1 detection ( ).

    Produced:

    Article Title: Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation
    Article Snippet: For co-transducing multiple genes, lentiviruses were produced separately and mixed at the time of transduction. .. At the time of 72 h after transduction, infected cells were selected by using 1.0–2.0 μg per ml of puromycin (P8833; Sigma-Aldrich, St Louis, MO) and/or 600–800 μg per ml G418 disulfate salt (A1720, Sigma-Aldrich, St Louis, MO).

    Construct:

    Article Title: Abnormal number cell division of human thyroid anaplastic carcinoma cell line, SW 1736
    Article Snippet: The eGFP-H2B plasmid was constructed by cloning of the human H2B DNA into plasmid pEGFP-C1 (Clontech Laboratories, Inc., Mounain View, CA, USA). .. The eGFP-H2B plasmid was transfected i\nto SW1736 human thyroid anaplastic carcinoma cells by electroporation with Gene Pulser II Electroporation System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the transfected cells were selected with G418 disulfate salt (Sigma-Aldrich Co., St. Louis, MO, USA).

    Article Title: Cerebrospinal fluid Presenilin-1 increases at asymptomatic stage in genetically determined Alzheimer’s disease
    Article Snippet: PS1 over-expressing cells silencing by siRNA CHO cells (400,000 cells/well) were grown in DMEM® (Gibco) containing 10 % Fetal Bovine Serum (Gibco) and 1 % Penicillin/Streptomycin (Sigma-Aldrich), and they were transfected with a construct encoding full-length PS1 (2 μg cDNA) [ ] or with the pcDNA3 expression plasmid alone (Invitrogen), using Lipofectamine 2000® (Invitrogen). .. CHO-PS1/APP cells (350,000 cells/well) were grown in DMEM® containing 10 % Fetal Bovine Serum, 0,1 % Puromicin (Sigma-Aldrich) and 0,2 % G418 disulfate salt (Sigma-Aldrich), were transfected with BLOCK iT™ Alexa Fluor® Red Fluorescent Oligo (Invitrogen) as control, or siRNA (50 nM) targeting human PS1 (Santa Cruz Biotechnology, INC).

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: .. The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. The HR reporter construct ‘DR-GFP’ [kindly provided by Dr. Maria Jasin; ( )] was integrated into the genomic DNA of MCF-7 cells by hygromycin selection (400 µg/ml; Invitrogen).

    Article Title: Epigenetic dysregulation of TET2 in human glioblastoma
    Article Snippet: TET2 transfection LN229 cell line was transfected in order to overexpress TET2 (GeneCopedia, EX-H3630-M43), incorparating a non-effective Scramble construct as negative control (GeneCopedia, EX-EGFP-M43), using Lipofectamine 3000 transfection Kit (Invitrogen, L3000015) as per the manufacturer´s recommendations. .. The cells were collected 72 h after transfection and stable transfectants were obtained after selection with 1 mg/ml G418 disulfate salt (Sigma, A-1720).

    Article Title: CD99 regulates neural differentiation of Ewing sarcoma cells through miR-34a-Notch-mediated control of NF-kB signaling
    Article Snippet: CAR-CTR-shRNA and CAR-CD99-shRNA clones were maintained in complete medium supplemented with 1000 μg/ml G418 disulfate salt (Sigma Aldrich). .. TC-71 cells stably expressing miR-34a (TCpMIF miR-34a) were obtained by infection with the feline immunodeficiency lentivirus-based construct pMIF-cGFPZeo-miR-34a (System Biosciences, Mountain View, CA, USA), as previously described .

    BAC Assay:

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich). .. The parental and mutant viruses were derived from EHV-1 strain Ab4, which was cloned as an infectious bacterial artificial chromosome (BAC).

    Marker:

    Article Title: PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
    Article Snippet: .. The NHEJ reporter construct ‘sGEJ’ was kindly provided by Dr. Ralph Scully ( ) and stably integrated into the genomic DNA of MCF-7 cells by using G418 disulfate salt (400 µg/ml; Sigma) as a selection marker. .. The HR reporter construct ‘DR-GFP’ [kindly provided by Dr. Maria Jasin; ( )] was integrated into the genomic DNA of MCF-7 cells by hygromycin selection (400 µg/ml; Invitrogen).

    Article Title: Equine Herpesvirus 1 Multiply Inserted Transmembrane Protein pUL43 Cooperates with pUL56 in Downregulation of Cell Surface Major Histocompatibility Complex Class I
    Article Snippet: RK13 cells that constitutively express either the ER marker calreticulin or the Golgi marker β-1,4-galactosyltransferase fused to enhanced green fluorescent protein (EGFP) were generated by transfection of the plasmids pER-EGFP and pGolgi-EGFP, respectively, which were kindly provided by Michael Veit (Freie Universität Berlin, Berlin, Germany). .. The transfected cell lines were purified and maintained in the medium for RK13 cells supplemented with 500 μg/ml G418 disulfate salt (Sigma-Aldrich).

    Variant Assay:

    Article Title: Quantitative Modulation of PpIX Fluorescence and Improved Glioma Visualization
    Article Snippet: The human GBM cell line U87wtEGFR overexpressing the EGFR gene, was generously provided by Prof. Dr. Frank Furnari (Laboratory of Tumor Biology, Ludwig Institute for Cancer Research, University of California-San Diego) and was cultured in DMEM GlutaMAX, 10% FBS and 1% penicillin-streptomycin, supplemented with 100 μg/mL of G418 disulfate salt (A1720, Sigma-Aldrich). .. U87MG vIII 4.12 cells stably expresses high level of the mutant EGFR variant III (deletion of exons 2-7) (CL 01004-CLTH, Tebu-bio) were maintained in DMEM GlutaMAX, 10% FBS, 1% penicillin-streptomycin and 0.2% of gentamicin 10 mg/mL (15710049, Thermo Fisher Scientific, Life Technologies Europe), enriched with 100 μg/mL of G418.

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    Millipore g418
    G418, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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