g418 disulfate salt  (Millipore)


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  • 98
    Name:
    G 418 disulfate salt
    Description:
    Chemical structure aminoglycoside
    Catalog Number:
    g5013
    Price:
    None
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    Structured Review

    Millipore g418 disulfate salt
    G 418 disulfate salt
    Chemical structure aminoglycoside
    https://www.bioz.com/result/g418 disulfate salt/product/Millipore
    Average 98 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    g418 disulfate salt - by Bioz Stars, 2020-08
    98/100 stars

    Images

    1) Product Images from "The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome"

    Article Title: The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00207-17

    Transient and permanent expression of the transferred DNA. (a) Overview of the experimental design to detect transient expression or stable integration of the transferred DNA. After infection of human cell lines with B. henselae , the DNA transferred through the T4SS will get to the nucleus where genes will be expressed. At 3 days postinfection, transient expression of gfp can be detected by flow cytometry. Antibiotic treatment was applied for long-term selection of neomycin-resistant colonies, to detect stable integration events. (b to d) Graphic representation of the percentage of GFP-positive cells obtained 3 days postinfection (b) and the number of G418-resistant colonies normalized for the number of cells at the beginning of the selection (c), as well as the Neo r /GFP + ratio (d). The different bars represented for each cell line correspond to the different relaxases under study, following the color code indicated in the squares at the top right. Data represent the means of the results from at least 3 independent experiments. Error bars indicate standard deviations. *, P
    Figure Legend Snippet: Transient and permanent expression of the transferred DNA. (a) Overview of the experimental design to detect transient expression or stable integration of the transferred DNA. After infection of human cell lines with B. henselae , the DNA transferred through the T4SS will get to the nucleus where genes will be expressed. At 3 days postinfection, transient expression of gfp can be detected by flow cytometry. Antibiotic treatment was applied for long-term selection of neomycin-resistant colonies, to detect stable integration events. (b to d) Graphic representation of the percentage of GFP-positive cells obtained 3 days postinfection (b) and the number of G418-resistant colonies normalized for the number of cells at the beginning of the selection (c), as well as the Neo r /GFP + ratio (d). The different bars represented for each cell line correspond to the different relaxases under study, following the color code indicated in the squares at the top right. Data represent the means of the results from at least 3 independent experiments. Error bars indicate standard deviations. *, P

    Techniques Used: Expressing, Infection, Flow Cytometry, Cytometry, Selection

    2) Product Images from "The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome"

    Article Title: The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00207-17

    Transient and permanent expression of the transferred DNA. (a) Overview of the experimental design to detect transient expression or stable integration of the transferred DNA. After infection of human cell lines with B. henselae , the DNA transferred through the T4SS will get to the nucleus where genes will be expressed. At 3 days postinfection, transient expression of gfp can be detected by flow cytometry. Antibiotic treatment was applied for long-term selection of neomycin-resistant colonies, to detect stable integration events. (b to d) Graphic representation of the percentage of GFP-positive cells obtained 3 days postinfection (b) and the number of G418-resistant colonies normalized for the number of cells at the beginning of the selection (c), as well as the Neo r /GFP + ratio (d). The different bars represented for each cell line correspond to the different relaxases under study, following the color code indicated in the squares at the top right. Data represent the means of the results from at least 3 independent experiments. Error bars indicate standard deviations. *, P
    Figure Legend Snippet: Transient and permanent expression of the transferred DNA. (a) Overview of the experimental design to detect transient expression or stable integration of the transferred DNA. After infection of human cell lines with B. henselae , the DNA transferred through the T4SS will get to the nucleus where genes will be expressed. At 3 days postinfection, transient expression of gfp can be detected by flow cytometry. Antibiotic treatment was applied for long-term selection of neomycin-resistant colonies, to detect stable integration events. (b to d) Graphic representation of the percentage of GFP-positive cells obtained 3 days postinfection (b) and the number of G418-resistant colonies normalized for the number of cells at the beginning of the selection (c), as well as the Neo r /GFP + ratio (d). The different bars represented for each cell line correspond to the different relaxases under study, following the color code indicated in the squares at the top right. Data represent the means of the results from at least 3 independent experiments. Error bars indicate standard deviations. *, P

    Techniques Used: Expressing, Infection, Flow Cytometry, Cytometry, Selection

    Related Articles

    Transfection:

    Article Title: The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer
    Article Snippet: .. For G418 (#G5013, Sigma) selection, cells were transfected with pEGFP-UBC13 plasmid or pGPH1-shUBC13 plasmid for 24 h and treated with 400 μg/mL G418 for 14 days. ..

    Selection:

    Article Title: The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer
    Article Snippet: .. For G418 (#G5013, Sigma) selection, cells were transfected with pEGFP-UBC13 plasmid or pGPH1-shUBC13 plasmid for 24 h and treated with 400 μg/mL G418 for 14 days. ..

    Article Title: The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome
    Article Snippet: .. At 72 hpi, G418 disulfate salt (Sigma-Aldrich) was added to infected cells, and selection was maintained for 4 to 5 weeks. ..

    Protease Inhibitor:

    Article Title: Miro proteins prime mitochondria for Parkin translocation and mitophagy
    Article Snippet: .. Media and reagents Reagents were from the following manufacturers: 35‐mm glass‐bottomed dishes (MatTek, P35G‐1.0‐14‐C), CELLview™ dishes (Greiner Bio‐One 627 871), poly‐L‐lysine (Sigma‐Aldrich, P6282), collagen IV (Sigma‐Aldrich, C5533), penicillin, streptomycin and amphotericin B (Invitrogen, 15240‐096), gentamicin (Krka, d. d., Novo Mesto, Slovakia), RPMI‐1640 (Life Technologies, A1049101), DMEM (Life Technologies, 31966021), Neurobasal™ A (Life Technologies, 10888022 and 12349015 (without phenol red)), HBSS 1640 (Life Technologies, 14025050), BME (Life Technologies 41010‐109), Opti‐MEM I (Life Technologies, 31985‐047), B‐27® Supplement (50X, serum free, Life Technologies, 17504044), foetal bovine serum (Life Technologies, 10270106), horse serum (Life Technologies, 26050088), GlutaMAX™‐I (Life Technologies, 3505038) Lipofectamine® 2000 (Invitrogen, 11668‐019), Metafectene® (Biontex Laboratories GmbH, T040), DMSO (Sigma‐Aldrich, 472301), FCCP (Tocris, 0453), G418 (Sigma‐Aldrich, A1720), MG132 (Tocris, 1748), protease inhibitor cocktail cOmplete (Roche, 04693116001), protein G‐sepharose 4B beads (Invitrogen, 101243), DC Protein Assay reagent (Bio‐Rad, 500‐0111), Pierce Lane Marker Reducing sample buffer (Thermo Fisher Scientific, 39000). .. Plasmids Plasmids expressing scrambled shRNA or shRNAs against mouse RHOT1 (KM35868N, suppresses efficiently also rat RHOT1), rat RHOT2 (KR44228N) and rat PINK1 (KR55105N) were from SABiosciences and validated earlier (Cagalinec et al , ; Choubey et al , ).

    Infection:

    Article Title: The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome
    Article Snippet: .. At 72 hpi, G418 disulfate salt (Sigma-Aldrich) was added to infected cells, and selection was maintained for 4 to 5 weeks. ..

    other:

    Article Title: Early-life exposure to low-dose oxidants can increase longevity via microbiome remodelling in Drosophila
    Article Snippet: G418 (Sigma, A1720) was dissolved in ethanol (Fisher Chemical, E/0650DF/15) as a 100× stock, and the control for G418 experiments was 1% ethanol in standard diet.

    Cell Culture:

    Article Title: Novel Method to Load Multiple Genes onto a Mammalian Artificial Chromosome
    Article Snippet: .. The 1D9-16 and RFPG18-12 cell lines were cultured in the presence of 400 µg/ml G418 (Sigma, G-5013). .. The RFPG18 cell line was cultured in the presence of 10 µM ganciclovir (Sigma, G-2536).

    Marker:

    Article Title: Miro proteins prime mitochondria for Parkin translocation and mitophagy
    Article Snippet: .. Media and reagents Reagents were from the following manufacturers: 35‐mm glass‐bottomed dishes (MatTek, P35G‐1.0‐14‐C), CELLview™ dishes (Greiner Bio‐One 627 871), poly‐L‐lysine (Sigma‐Aldrich, P6282), collagen IV (Sigma‐Aldrich, C5533), penicillin, streptomycin and amphotericin B (Invitrogen, 15240‐096), gentamicin (Krka, d. d., Novo Mesto, Slovakia), RPMI‐1640 (Life Technologies, A1049101), DMEM (Life Technologies, 31966021), Neurobasal™ A (Life Technologies, 10888022 and 12349015 (without phenol red)), HBSS 1640 (Life Technologies, 14025050), BME (Life Technologies 41010‐109), Opti‐MEM I (Life Technologies, 31985‐047), B‐27® Supplement (50X, serum free, Life Technologies, 17504044), foetal bovine serum (Life Technologies, 10270106), horse serum (Life Technologies, 26050088), GlutaMAX™‐I (Life Technologies, 3505038) Lipofectamine® 2000 (Invitrogen, 11668‐019), Metafectene® (Biontex Laboratories GmbH, T040), DMSO (Sigma‐Aldrich, 472301), FCCP (Tocris, 0453), G418 (Sigma‐Aldrich, A1720), MG132 (Tocris, 1748), protease inhibitor cocktail cOmplete (Roche, 04693116001), protein G‐sepharose 4B beads (Invitrogen, 101243), DC Protein Assay reagent (Bio‐Rad, 500‐0111), Pierce Lane Marker Reducing sample buffer (Thermo Fisher Scientific, 39000). .. Plasmids Plasmids expressing scrambled shRNA or shRNAs against mouse RHOT1 (KM35868N, suppresses efficiently also rat RHOT1), rat RHOT2 (KR44228N) and rat PINK1 (KR55105N) were from SABiosciences and validated earlier (Cagalinec et al , ; Choubey et al , ).

    Plasmid Preparation:

    Article Title: The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer
    Article Snippet: .. For G418 (#G5013, Sigma) selection, cells were transfected with pEGFP-UBC13 plasmid or pGPH1-shUBC13 plasmid for 24 h and treated with 400 μg/mL G418 for 14 days. ..

    DC Protein Assay:

    Article Title: Miro proteins prime mitochondria for Parkin translocation and mitophagy
    Article Snippet: .. Media and reagents Reagents were from the following manufacturers: 35‐mm glass‐bottomed dishes (MatTek, P35G‐1.0‐14‐C), CELLview™ dishes (Greiner Bio‐One 627 871), poly‐L‐lysine (Sigma‐Aldrich, P6282), collagen IV (Sigma‐Aldrich, C5533), penicillin, streptomycin and amphotericin B (Invitrogen, 15240‐096), gentamicin (Krka, d. d., Novo Mesto, Slovakia), RPMI‐1640 (Life Technologies, A1049101), DMEM (Life Technologies, 31966021), Neurobasal™ A (Life Technologies, 10888022 and 12349015 (without phenol red)), HBSS 1640 (Life Technologies, 14025050), BME (Life Technologies 41010‐109), Opti‐MEM I (Life Technologies, 31985‐047), B‐27® Supplement (50X, serum free, Life Technologies, 17504044), foetal bovine serum (Life Technologies, 10270106), horse serum (Life Technologies, 26050088), GlutaMAX™‐I (Life Technologies, 3505038) Lipofectamine® 2000 (Invitrogen, 11668‐019), Metafectene® (Biontex Laboratories GmbH, T040), DMSO (Sigma‐Aldrich, 472301), FCCP (Tocris, 0453), G418 (Sigma‐Aldrich, A1720), MG132 (Tocris, 1748), protease inhibitor cocktail cOmplete (Roche, 04693116001), protein G‐sepharose 4B beads (Invitrogen, 101243), DC Protein Assay reagent (Bio‐Rad, 500‐0111), Pierce Lane Marker Reducing sample buffer (Thermo Fisher Scientific, 39000). .. Plasmids Plasmids expressing scrambled shRNA or shRNAs against mouse RHOT1 (KM35868N, suppresses efficiently also rat RHOT1), rat RHOT2 (KR44228N) and rat PINK1 (KR55105N) were from SABiosciences and validated earlier (Cagalinec et al , ; Choubey et al , ).

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  • 99
    Millipore g418 plates
    Generation and verification of tagged protein arrays. ( A ) To tag ORFX as bait (V5–6×HIS) and prey (V5–3×VSV), a set of primers is used that anneal to identical binding sites within the template plasmids and have flanking sequence homologous to ORFX . PCR products generated from the bait and prey templates are transformed into a - and α-cells, respectively. Homologous recombination occurs between the variable portion of the 5′ primer (light blue) and the 3′ terminus of the ORF, and between the variable portion of the 3′ primer (red) and the 3′ UTR) of ORFX . Transformants are selected on <t>G418</t> plates, and colony PCR is performed to verify integration of the Kan r downstream of the desired ORF. Abbreviations: TEF, translational elongation factor; TEFp, TEF promoter; TEFt, TEF terminator: Kan r , kanamycin resistance; loxp, site for CRE specific homologous recombination. ( B . The asterisk (*) denotes possible misloading or protein degradation. Note in the RAD51 lane the multiple protein products. Expected protein sizes are listed in Supplemental Table 1. ( C ) Analysis of effects on cell growth by tagging essential genes. A total of 24 strains with essential genes tagged as baits (6×HIS) and preys (3×VSV) were grown to saturation and spotted in 10-fold dilutions on YPD. Pictures were taken after 2 d at 30°C.
    G418 Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g418 plates/product/Millipore
    Average 99 stars, based on 2660 article reviews
    Price from $9.99 to $1999.99
    g418 plates - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Millipore g418 resistant cos 7 cells
    ( A ) Detection of wild-type and mutant ASM proteins following transfection in <t>COS-7</t> cells. Wild-type and mutant SMPD1 cDNAs in pCMV6 were transfected in COS-7 cells and selected in the presence of 400 μg/ml <t>G418.</t> G418-resistant clones expressing
    G418 Resistant Cos 7 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g418 resistant cos 7 cells/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g418 resistant cos 7 cells - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Generation and verification of tagged protein arrays. ( A ) To tag ORFX as bait (V5–6×HIS) and prey (V5–3×VSV), a set of primers is used that anneal to identical binding sites within the template plasmids and have flanking sequence homologous to ORFX . PCR products generated from the bait and prey templates are transformed into a - and α-cells, respectively. Homologous recombination occurs between the variable portion of the 5′ primer (light blue) and the 3′ terminus of the ORF, and between the variable portion of the 3′ primer (red) and the 3′ UTR) of ORFX . Transformants are selected on G418 plates, and colony PCR is performed to verify integration of the Kan r downstream of the desired ORF. Abbreviations: TEF, translational elongation factor; TEFp, TEF promoter; TEFt, TEF terminator: Kan r , kanamycin resistance; loxp, site for CRE specific homologous recombination. ( B . The asterisk (*) denotes possible misloading or protein degradation. Note in the RAD51 lane the multiple protein products. Expected protein sizes are listed in Supplemental Table 1. ( C ) Analysis of effects on cell growth by tagging essential genes. A total of 24 strains with essential genes tagged as baits (6×HIS) and preys (3×VSV) were grown to saturation and spotted in 10-fold dilutions on YPD. Pictures were taken after 2 d at 30°C.

    Journal: Genome Research

    Article Title: Examining protein-protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system

    doi: 10.1101/gr.6667007

    Figure Lengend Snippet: Generation and verification of tagged protein arrays. ( A ) To tag ORFX as bait (V5–6×HIS) and prey (V5–3×VSV), a set of primers is used that anneal to identical binding sites within the template plasmids and have flanking sequence homologous to ORFX . PCR products generated from the bait and prey templates are transformed into a - and α-cells, respectively. Homologous recombination occurs between the variable portion of the 5′ primer (light blue) and the 3′ terminus of the ORF, and between the variable portion of the 3′ primer (red) and the 3′ UTR) of ORFX . Transformants are selected on G418 plates, and colony PCR is performed to verify integration of the Kan r downstream of the desired ORF. Abbreviations: TEF, translational elongation factor; TEFp, TEF promoter; TEFt, TEF terminator: Kan r , kanamycin resistance; loxp, site for CRE specific homologous recombination. ( B . The asterisk (*) denotes possible misloading or protein degradation. Note in the RAD51 lane the multiple protein products. Expected protein sizes are listed in Supplemental Table 1. ( C ) Analysis of effects on cell growth by tagging essential genes. A total of 24 strains with essential genes tagged as baits (6×HIS) and preys (3×VSV) were grown to saturation and spotted in 10-fold dilutions on YPD. Pictures were taken after 2 d at 30°C.

    Article Snippet: Transformants that grew on the G418 plates were restreaked and tested for proper integration of the tagging cassette via colony PCR.

    Techniques: Binding Assay, Sequencing, Polymerase Chain Reaction, Generated, Transformation Assay, Homologous Recombination

    Transformation of pZLY1 into Candida shehatae 20335 and detection of the dominant selective marker (G418 resistance) and reporter genes (GFP gene). A. 1: Colonies were not observed on YEPX solid medium with G418 when Candida shehatae 20335 was not transformed by pZLY1; 2: several colonies grew on YEPX solid medium with G418 when Candida shehatae 20335 was transformed by pZLY1. B. Candida shehatae 20335 cells were observed under light microscopy (1600×). C. Candida shehatae 20335 transformants were observed under light microscopy (1600×). No morphological differences can be seen between the cells. D. Candida shehatae 20335 cells observed under fluorescence microscopy (1000×), showing GFP is not expressed in untransformed cells. E. Candida shehatae 20335 cells observed under fluorescence microscopy (1000×), showing GFP is expressed in transformants.

    Journal: PLoS ONE

    Article Title: Construction and Analysis of High-Ethanol-Producing Fusants with Co-Fermentation Ability through Protoplast Fusion and Double Labeling Technology

    doi: 10.1371/journal.pone.0108311

    Figure Lengend Snippet: Transformation of pZLY1 into Candida shehatae 20335 and detection of the dominant selective marker (G418 resistance) and reporter genes (GFP gene). A. 1: Colonies were not observed on YEPX solid medium with G418 when Candida shehatae 20335 was not transformed by pZLY1; 2: several colonies grew on YEPX solid medium with G418 when Candida shehatae 20335 was transformed by pZLY1. B. Candida shehatae 20335 cells were observed under light microscopy (1600×). C. Candida shehatae 20335 transformants were observed under light microscopy (1600×). No morphological differences can be seen between the cells. D. Candida shehatae 20335 cells observed under fluorescence microscopy (1000×), showing GFP is not expressed in untransformed cells. E. Candida shehatae 20335 cells observed under fluorescence microscopy (1000×), showing GFP is expressed in transformants.

    Article Snippet: Fusants were inoculated into 20 mL/50 mL YEPD liquid medium without G418 and blasticidin, incubated overnight at 30°C with a shaking speed of 140 rpm, subcultured the obtained yeast broth five times and planted on YEPD solid medium with and without G418 and blasticidin.

    Techniques: Transformation Assay, Marker, Light Microscopy, Fluorescence, Microscopy

    Screening of protoplast fusants. A. Many colonies are found to grow on YEPDS regenerated solid medium when protoplast fusants are spread on it. B and C. Several colonies grow on YEPDS regenerated solid medium with blasticidin and G418.

    Journal: PLoS ONE

    Article Title: Construction and Analysis of High-Ethanol-Producing Fusants with Co-Fermentation Ability through Protoplast Fusion and Double Labeling Technology

    doi: 10.1371/journal.pone.0108311

    Figure Lengend Snippet: Screening of protoplast fusants. A. Many colonies are found to grow on YEPDS regenerated solid medium when protoplast fusants are spread on it. B and C. Several colonies grow on YEPDS regenerated solid medium with blasticidin and G418.

    Article Snippet: Fusants were inoculated into 20 mL/50 mL YEPD liquid medium without G418 and blasticidin, incubated overnight at 30°C with a shaking speed of 140 rpm, subcultured the obtained yeast broth five times and planted on YEPD solid medium with and without G418 and blasticidin.

    Techniques:

    Structures of the pZLY1 plasmid (left) with the G418 resistance gene ( Kan R) and adh 1P– GFP – adh 1t reporter gene, and the pZLY2 plasmid (right) with the blasticidin resistance gene ( bsd ) and adh 1P– gus A– adh 1t reporter gene. adh 1P and adh 1t represent, respectively, the promoter and terminator of the adh 1 gene of Saccharomyces cerevisiae . Kan R represents the G418 resistance gene, gus A represents the gus A gene and GFP represent the GFP gene.

    Journal: PLoS ONE

    Article Title: Construction and Analysis of High-Ethanol-Producing Fusants with Co-Fermentation Ability through Protoplast Fusion and Double Labeling Technology

    doi: 10.1371/journal.pone.0108311

    Figure Lengend Snippet: Structures of the pZLY1 plasmid (left) with the G418 resistance gene ( Kan R) and adh 1P– GFP – adh 1t reporter gene, and the pZLY2 plasmid (right) with the blasticidin resistance gene ( bsd ) and adh 1P– gus A– adh 1t reporter gene. adh 1P and adh 1t represent, respectively, the promoter and terminator of the adh 1 gene of Saccharomyces cerevisiae . Kan R represents the G418 resistance gene, gus A represents the gus A gene and GFP represent the GFP gene.

    Article Snippet: Fusants were inoculated into 20 mL/50 mL YEPD liquid medium without G418 and blasticidin, incubated overnight at 30°C with a shaking speed of 140 rpm, subcultured the obtained yeast broth five times and planted on YEPD solid medium with and without G418 and blasticidin.

    Techniques: Plasmid Preparation

    ( A ) Detection of wild-type and mutant ASM proteins following transfection in COS-7 cells. Wild-type and mutant SMPD1 cDNAs in pCMV6 were transfected in COS-7 cells and selected in the presence of 400 μg/ml G418. G418-resistant clones expressing

    Journal: JIMD Reports

    Article Title: Molecular Genetic Characterization of Novel Sphingomyelin Phosphodiesterase 1 Mutations Causing Niemann-Pick Disease

    doi: 10.1007/8904_2011_80

    Figure Lengend Snippet: ( A ) Detection of wild-type and mutant ASM proteins following transfection in COS-7 cells. Wild-type and mutant SMPD1 cDNAs in pCMV6 were transfected in COS-7 cells and selected in the presence of 400 μg/ml G418. G418-resistant clones expressing

    Article Snippet: Protein extracts from transfected and G418-resistant COS-7 cells (50 μg protein/lane) were subjected to sodium dodecyl sulfate denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (Millipore Corp, Billerica, MA).

    Techniques: Mutagenesis, Transfection, Clone Assay, Expressing