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Agilent technologies g2567aa microarray scanner system
G2567aa Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g2567aa microarray scanner system - by Bioz Stars, 2020-03
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Microarray:

Article Title: CCAAT/enhancer binding protein β directly regulates the expression of the complement component 3 gene in neural cells: implications for the pro-inflammatory effects of this transcription factor
Article Snippet: .. The chips were scanned using the Agilent G2567AA Microarray Scanner System (Agilent Technologies, Madrid, Spain). .. Image analysis and data collection were carried out using the Agilent Feature Extraction 9.1.3.1. (AFE, Agilent Technologies, Madrid, Spain).

Mouse Assay:

Article Title: CCAAT/enhancer binding protein β directly regulates the expression of the complement component 3 gene in neural cells: implications for the pro-inflammatory effects of this transcription factor
Article Snippet: Microarray analysis Total RNA was extracted from hippocampus of C/EBPβ +/+ and C/EBPβ −/− mice using Trizol (Agilent Technologies, Madrid, Spain) and the integrity of RNA confirmed in an Agilent 2100 Bioanalyzer. .. The chips were scanned using the Agilent G2567AA Microarray Scanner System (Agilent Technologies, Madrid, Spain).

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    Agilent technologies high resolution microarray scanner
    BDNF induces the dissociation of mRNA from hnRNP K at the synapse. A , B , Effect of BDNF stimulation (50 ng/ml; 10 min) on transcript coimmunoprecipitation with hnRNP K in cultured hippocampal neurons as assessed by <t>microarray</t> and qPCR. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. A , Genes coding for synaptic proteins: GluA1, AMPA receptor subunit 1; GluA2, AMPA receptor subunit 2; GluN1, NMDAR subunit 1. B , Other genes: TrkB, tropomyosin-related kinase B receptor; NPAS4, neuronal PAS domain protein 4. The results represent quantitation of four different experiments performed in independent preparations, and are expressed as percentage [mean ± SEM (qPCR) or SD (microarray)] of control; * p
    High Resolution Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution microarray scanner/product/Agilent technologies
    Average 99 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    high resolution microarray scanner - by Bioz Stars, 2020-03
    99/100 stars
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    79
    Agilent technologies dual laser dna microarray scanner model g2565aa
    Scatter plot of differentially expressed genes and heat map representation of <t>microarray</t> data for proapoptotic and <t>DNA</t> repair of differentially expressed genes. A , scatter plot of differentially expressed genes from colon mucosa from FD β- pol
    Dual Laser Dna Microarray Scanner Model G2565aa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual laser dna microarray scanner model g2565aa/product/Agilent technologies
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dual laser dna microarray scanner model g2565aa - by Bioz Stars, 2020-03
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    89
    Agilent technologies g2565aa dual laser microarray scanner
    Scatter plot of differentially expressed genes and heat map representation of <t>microarray</t> data for proapoptotic and <t>DNA</t> repair of differentially expressed genes. A , scatter plot of differentially expressed genes from colon mucosa from FD β- pol
    G2565aa Dual Laser Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g2565aa dual laser microarray scanner/product/Agilent technologies
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g2565aa dual laser microarray scanner - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

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    BDNF induces the dissociation of mRNA from hnRNP K at the synapse. A , B , Effect of BDNF stimulation (50 ng/ml; 10 min) on transcript coimmunoprecipitation with hnRNP K in cultured hippocampal neurons as assessed by microarray and qPCR. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. A , Genes coding for synaptic proteins: GluA1, AMPA receptor subunit 1; GluA2, AMPA receptor subunit 2; GluN1, NMDAR subunit 1. B , Other genes: TrkB, tropomyosin-related kinase B receptor; NPAS4, neuronal PAS domain protein 4. The results represent quantitation of four different experiments performed in independent preparations, and are expressed as percentage [mean ± SEM (qPCR) or SD (microarray)] of control; * p

    Journal: eNeuro

    Article Title: The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons

    doi: 10.1523/ENEURO.0268-17.2017

    Figure Lengend Snippet: BDNF induces the dissociation of mRNA from hnRNP K at the synapse. A , B , Effect of BDNF stimulation (50 ng/ml; 10 min) on transcript coimmunoprecipitation with hnRNP K in cultured hippocampal neurons as assessed by microarray and qPCR. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. A , Genes coding for synaptic proteins: GluA1, AMPA receptor subunit 1; GluA2, AMPA receptor subunit 2; GluN1, NMDAR subunit 1. B , Other genes: TrkB, tropomyosin-related kinase B receptor; NPAS4, neuronal PAS domain protein 4. The results represent quantitation of four different experiments performed in independent preparations, and are expressed as percentage [mean ± SEM (qPCR) or SD (microarray)] of control; * p

    Article Snippet: The Whole Rat Genome Microarray kit (4 × 44K, Agilent Technologies) was used and analyzed with a high-resolution microarray scanner (G2565AA, Agilent Technologies). hnRNP K-bound mRNAs were identified by setting a cutoff value of the fold variation between the hnRNP K and IgG samples.

    Techniques: Cell Culture, Microarray, Real-time Polymerase Chain Reaction, Quantitation Assay

    Stimulation of hippocampal neurons with BDNF decreases the interaction of hnRNP K with a large number of transcripts. A , B , List of the 10 most significantly enriched biological processes associated with mRNAs bound to hnRNP K ( A ) and those that were regulated by BDNF (50 ng/ml for 10 min) ( B ), identified with the PANTHER classification system. Only categories showing at least a 2-fold enrichment (considering the size of our lists) were analyzed and the 10 categories displaying the highest –log 10 ( p value) are shown. The number of transcripts belonging to each category and the fold change (designated in parenthesis) are indicated within graph bars. reg., regulation; pres., presynaptic; chem, chemical; syn., synaptic; pos., positive; proj., projection; diff., differentiation; neg., negative; sys., system; devel., development. C , Cultured hippocampal neurons were stimulated or not with BDNF (50 ng/ml) for 10 min before preparation of cellular extracts. hnRNP K was immunoprecipitated from control and BDNF-treated hippocampal neuron homogenates, and the associated transcripts were identified by microarray analysis. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. The results were obtained from the quantification of four different experiments performed in independent preparations, and are expressed as -log ( p value) and log fold change (BDNF vs control). A total of 9509 transcripts showed a decrease in the interaction with hnRNP K in cells stimulated with BDNF; p

    Journal: eNeuro

    Article Title: The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons

    doi: 10.1523/ENEURO.0268-17.2017

    Figure Lengend Snippet: Stimulation of hippocampal neurons with BDNF decreases the interaction of hnRNP K with a large number of transcripts. A , B , List of the 10 most significantly enriched biological processes associated with mRNAs bound to hnRNP K ( A ) and those that were regulated by BDNF (50 ng/ml for 10 min) ( B ), identified with the PANTHER classification system. Only categories showing at least a 2-fold enrichment (considering the size of our lists) were analyzed and the 10 categories displaying the highest –log 10 ( p value) are shown. The number of transcripts belonging to each category and the fold change (designated in parenthesis) are indicated within graph bars. reg., regulation; pres., presynaptic; chem, chemical; syn., synaptic; pos., positive; proj., projection; diff., differentiation; neg., negative; sys., system; devel., development. C , Cultured hippocampal neurons were stimulated or not with BDNF (50 ng/ml) for 10 min before preparation of cellular extracts. hnRNP K was immunoprecipitated from control and BDNF-treated hippocampal neuron homogenates, and the associated transcripts were identified by microarray analysis. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. The results were obtained from the quantification of four different experiments performed in independent preparations, and are expressed as -log ( p value) and log fold change (BDNF vs control). A total of 9509 transcripts showed a decrease in the interaction with hnRNP K in cells stimulated with BDNF; p

    Article Snippet: The Whole Rat Genome Microarray kit (4 × 44K, Agilent Technologies) was used and analyzed with a high-resolution microarray scanner (G2565AA, Agilent Technologies). hnRNP K-bound mRNAs were identified by setting a cutoff value of the fold variation between the hnRNP K and IgG samples.

    Techniques: Cell Culture, Immunoprecipitation, Microarray

    Network based analysis of genes affected by CD40:Fc treatment. Microarray analysis was performed on SG of CD40:Fc treated and control mice (n = 4 each, treatment at 10 weeks, end of study at 20 weeks) and gene changes greater than 2 fold were used in the analysis and compared for biological functional pathways or biomarkers by an Ingenuity Pathway analysis (IPA). ( A ) The pathway of NF-κB and TGF-β was affected in CD40:Fc treated mice. Green colored shapes indicates upregulated genes, red colored shapes downregulated; gray colored shapes genes in the pathway that were not differentially expressed. Color intensity reflects the degree of differential expression. Triangles represent phosphatases; horizontal diamond, peptidases; vertical diamond, enzymes; horizontal oval, transcription factors; vertical oval, transmembrane receptors; trapezoid, transporters; circle, other type of protein. Dotted lines indicate an indirect interaction; solid line, direct interaction, solid arrow head indicates “acts on”. ( B ) Metacore analysis of pathways linking differentially expressed genes to immune pathways. The differentially expressed genes are indicated along with the direction of change in expression. ( C ) Quantitative-PCR of selected genes shows agreement with microarray results. The results obtained using the microarray platform were validated by examining the correlation between the expression levels in the microarray and qPCR results obtained for a subset of genes.

    Journal: PLoS ONE

    Article Title: Local Administration of Soluble CD40:Fc to the Salivary Glands of Non-Obese Diabetic Mice Does Not Ameliorate Autoimmune Inflammation

    doi: 10.1371/journal.pone.0051375

    Figure Lengend Snippet: Network based analysis of genes affected by CD40:Fc treatment. Microarray analysis was performed on SG of CD40:Fc treated and control mice (n = 4 each, treatment at 10 weeks, end of study at 20 weeks) and gene changes greater than 2 fold were used in the analysis and compared for biological functional pathways or biomarkers by an Ingenuity Pathway analysis (IPA). ( A ) The pathway of NF-κB and TGF-β was affected in CD40:Fc treated mice. Green colored shapes indicates upregulated genes, red colored shapes downregulated; gray colored shapes genes in the pathway that were not differentially expressed. Color intensity reflects the degree of differential expression. Triangles represent phosphatases; horizontal diamond, peptidases; vertical diamond, enzymes; horizontal oval, transcription factors; vertical oval, transmembrane receptors; trapezoid, transporters; circle, other type of protein. Dotted lines indicate an indirect interaction; solid line, direct interaction, solid arrow head indicates “acts on”. ( B ) Metacore analysis of pathways linking differentially expressed genes to immune pathways. The differentially expressed genes are indicated along with the direction of change in expression. ( C ) Quantitative-PCR of selected genes shows agreement with microarray results. The results obtained using the microarray platform were validated by examining the correlation between the expression levels in the microarray and qPCR results obtained for a subset of genes.

    Article Snippet: Following the washing procedures, the slides were immediately scanned using a Microarray Scanner (Model: Agilent G2565AA System) to minimize the environmental oxidation and loss of signal intensities.

    Techniques: Microarray, Mouse Assay, Functional Assay, Indirect Immunoperoxidase Assay, Expressing, Real-time Polymerase Chain Reaction

    Scatter plot of differentially expressed genes and heat map representation of microarray data for proapoptotic and DNA repair of differentially expressed genes. A , scatter plot of differentially expressed genes from colon mucosa from FD β- pol

    Journal: The Journal of Biological Chemistry

    Article Title: Folate Deficiency Provides Protection against Colon Carcinogenesis in DNA Polymerase ? Haploinsufficient Mice *

    doi: 10.1074/jbc.M109.069807

    Figure Lengend Snippet: Scatter plot of differentially expressed genes and heat map representation of microarray data for proapoptotic and DNA repair of differentially expressed genes. A , scatter plot of differentially expressed genes from colon mucosa from FD β- pol

    Article Snippet: Microarrays were scanned using the Agilent dual laser DNA microarray scanner model G2565AA, with 10-μm resolution.

    Techniques: Microarray