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Agilent technologies g2565ca scanner system
G2565ca Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/g2565ca scanner system/product/Agilent technologies
Average 93 stars, based on 11 article reviews
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g2565ca scanner system - by Bioz Stars, 2020-04
93/100 stars

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Hybridization:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: After hybridization, microarrays were washed and then dried immediately. .. After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range.

Article Title: CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands
Article Snippet: After hybridization, the microarrays were washed and then immediately dried. .. After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: For each sample, hybridization was performed using 600 ng of Cy3-labeled cRNA (specific activity > 6.0 pmol of Cy3/μg of cRNA) during 17 h at 65 °C, following the manufacturer’s protocol at the CRB GADIE facility (INRA Jouy-en-Josas, France, http://crb-gadie.inra.fr /). .. The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies).

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: Paragraph title: 4.5.2. Microarray Hybridization and Transcriptome Data Analysist ... Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies).

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: After hybridization, the microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent Technologies) and 1 minute at 37 °C using GE Wash Buffer 2 (Agilent Technologies). .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: For each sample (n = 61), a total amount of 100 ng of total RNA was dephosphorylated, 3′ end-labelled with Cy3-pCp, purified on Micro Bio-Spin 6 columns (Bio-Rad, USA), dried, and hybridized to the arrays using the miRNA Complete Labeling and Hybridization Kit (Agilent Technologies) according to the manufacturer’s instructions. .. After a washing step, hybridized microarray slides were scanned using a G2565CA Scanner System (Agilent Technologies) and a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: After hybridization, microarrays were washed 1 minute at room temperature with the GE Wash Buffer 1 (Agilent Technologies) and 1 minute at 37°C using the GE Wash Buffer 2 (Agilent Technologies), then dried instantly. .. Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus
Article Snippet: After hybridization, microarrays were washed for 1 minute at room temperature with the GE Wash Buffer 1 (Agilent Technologies) and 1 minute at 37 °C using the GE Wash Buffer 2 (Agilent Technologies). .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 µm and a dynamic range of 20 bit.

Functional Assay:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range. .. Identification of differentially expressed genes and functional investigations were done using GeneSpring software (version 14.8; Agilent Technologies).

Microarray:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range. .. MIAME (minimum information about microarray experiment)-compliant data were deposited in ArrayExpress at EMBL.

Article Title: CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands
Article Snippet: Paragraph title: Gene expression by microarray analyses ... After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: After isopropanol precipitation, the RNA concentration was determined using a NanoDrop ND-1000 spectrophotometer (Nyxor Biotech, Paris, France) and RNA integrity was checked on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). b) Microarray hybridization and processing A customized 60 K bovine microarray (Agilent Technologies, Santa Clara, CA, USA, GEO profile GPL21724) covering more than 97% of Ensembl Bos taurus transcripts (genome assembly UMD3.1) was used. .. The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies).

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: Paragraph title: 4.5.2. Microarray Hybridization and Transcriptome Data Analysist ... Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies).

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: Paragraph title: Blood samples, RNA isolation and microarray experiments ... The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: .. After a washing step, hybridized microarray slides were scanned using a G2565CA Scanner System (Agilent Technologies) and a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits. .. The resulting TIFF images were analysed with the Feature Extraction Software v10.7.3.1 (Agilent Technologies), using the miRNA_107_Sep09 protocol.

Article Title: Thermal manipulation of the chicken embryo triggers differential gene expression in response to a later heat challenge
Article Snippet: Paragraph title: Microarray experiments ... The slides were finally scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a 20 bit dynamic range.

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: Paragraph title: RNA labeling and microarray processing ... Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus
Article Snippet: Paragraph title: Microarray hybridizations ... The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 µm and a dynamic range of 20 bit.

Isolation:

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: Paragraph title: Blood samples, RNA isolation and microarray experiments ... The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Infection:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: A single-color design was used to provide two types of comparisons: (i)IgG1-treated mice versus IL-22-Fc-treated mice, and (ii) PBS-treated infected mice versus IgG1-treated infected mice versus IL-22-Fc-treated infected mice. .. After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range.

Mouse Assay:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: A single-color design was used to provide two types of comparisons: (i)IgG1-treated mice versus IL-22-Fc-treated mice, and (ii) PBS-treated infected mice versus IgG1-treated infected mice versus IL-22-Fc-treated infected mice. .. After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range.

Labeling:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: Cy3-labeled cRNAs were prepared from 100 ng of total RNA using a one-color Low Input Quick Amp labeling kit (Agilent Technologies). .. After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range.

Article Title: CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands
Article Snippet: Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100 ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies), following the recommended protocol. .. After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: Cyanine-3 (Cy3) labeled cRNA were prepared with 200 ng of total RNA using the One-Color Low Input Quick Amp Labeling kit following the recommended protocol (Agilent Technologies, Santa Clara, CA, USA). .. The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies).

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: Cyanine-3 (Cy3) labeled cRNA were prepared with 200 ng of total RNA using the One-Color Low Input Quick Amp Labeling kit following the recommended protocol (Agilent Technologies). .. Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies).

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: Cyanine-3 (Cy3)-labelled cRNAs were prepared from 100 ng of total RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: For each sample (n = 61), a total amount of 100 ng of total RNA was dephosphorylated, 3′ end-labelled with Cy3-pCp, purified on Micro Bio-Spin 6 columns (Bio-Rad, USA), dried, and hybridized to the arrays using the miRNA Complete Labeling and Hybridization Kit (Agilent Technologies) according to the manufacturer’s instructions. .. After a washing step, hybridized microarray slides were scanned using a G2565CA Scanner System (Agilent Technologies) and a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: Paragraph title: RNA labeling and microarray processing ... Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus
Article Snippet: Cyanine-3 (Cy3) labeled cRNAs were prepared from whole cell and RISC-IP RNAs using the One-Color Low Input Quick Amp Labeling kit (Agilent Technologies, Santa Clara, CA, USA), The starting RNA amount was 50 ng and 10 ng for whole cell and RISC-IP RNAs, respectively. .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 µm and a dynamic range of 20 bit.

Concentration Assay:

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: After isopropanol precipitation, the RNA concentration was determined using a NanoDrop ND-1000 spectrophotometer (Nyxor Biotech, Paris, France) and RNA integrity was checked on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). b) Microarray hybridization and processing A customized 60 K bovine microarray (Agilent Technologies, Santa Clara, CA, USA, GEO profile GPL21724) covering more than 97% of Ensembl Bos taurus transcripts (genome assembly UMD3.1) was used. .. The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies).

Activity Assay:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: For each sample, 600 ng of Cy3-labeled cRNA (specific activity of > 9 pmol of Cy3/μg of cRNA) was fragmented at 60°C for 30 min. cRNA was hybridized to the microarrays for 17 h at 65°C in a rotating hybridization oven as previously described ( ). .. After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range.

Article Title: CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands
Article Snippet: For each sample, 600 ng of Cy3-labeled cRNA (specific activity > 11.0 pmol Cy3/μg of cRNA) were fragmented at 60 °C for 30 min and hybridized to the microarrays for 17 h at 65 °C in a rotating hybridization oven (Agilent Technologies). .. After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: For each sample, hybridization was performed using 600 ng of Cy3-labeled cRNA (specific activity > 6.0 pmol of Cy3/μg of cRNA) during 17 h at 65 °C, following the manufacturer’s protocol at the CRB GADIE facility (INRA Jouy-en-Josas, France, http://crb-gadie.inra.fr /). .. The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies).

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: For each sample, hybridization was performed using 600 ng of Cy3-labeled cRNA (specific activity > 6.0 pmol of Cy3/μg of cRNA) for 17 h at 65 °C, following the manufacturer’s protocol at the Animal Biological Resources for Integrative and Digital Genomics facility (INRA Jouy-en-Josas, France, http://crb-gadie.inra.fr/ ). .. Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies).

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: For each sample, 1.65 μg of Cy3-labeled cRNA (specific activity > 9.0 pmol Cy3/μg of cRNA) were fragmented at 60 °C for 30 minutes in a volume of 55 μl containing 25× Agilent Fragmentation Buffer and 10× Agilent Blocking Agent. .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Thermal manipulation of the chicken embryo triggers differential gene expression in response to a later heat challenge
Article Snippet: Three hundred ng of Cy3-labeled cRNA (specific activity > 9) and 300 ng of Cy5-labeled cRNA (specific activity > 9) were combined for each single array, and the washing steps were then performed according to the manufacturer’s recommendations. .. The slides were finally scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a 20 bit dynamic range.

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: For each sample, 600 ng of Cy3-labeled cRNA (specific activity > 6.0 pmol Cy3/μg of cRNA) were fragmented at 60°C for 30 minutes in a reaction volume of 25 μl containing 25× Agilent Fragmentation Buffer and 10× Agilent Blocking Agent, following the manufacturer’s instructions. .. Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Spectrophotometry:

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: After isopropanol precipitation, the RNA concentration was determined using a NanoDrop ND-1000 spectrophotometer (Nyxor Biotech, Paris, France) and RNA integrity was checked on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). b) Microarray hybridization and processing A customized 60 K bovine microarray (Agilent Technologies, Santa Clara, CA, USA, GEO profile GPL21724) covering more than 97% of Ensembl Bos taurus transcripts (genome assembly UMD3.1) was used. .. The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies).

Expressing:

Article Title: CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands
Article Snippet: Paragraph title: Gene expression by microarray analyses ... After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies). .. The microarray data were submitted to the public repository Gene Expression Omnibus (GEO accession number GSE94338). c) Analysis of microarray expression data Raw data were normalized through intra-array median subtraction and log2 transformation using R statistical software [ ].

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies). .. Raw data were normalized through intra-array median subtraction and log2 transformation, and then, the expression values were analyzed with a mixture model variance (VarMixt) method (R software).

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits. .. The microarray data can be obtained from the Gene Expression Omnibus (GEO) database with the accession number GSE72973 ( http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc = GSE72973 ).

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: Subsequently, 25 μl of 2× Agilent Hybridization Buffer were added to the fragmentation mixture and hybridized to the Rabbit Custom Gene Expression Microarrays (Agilent Technologies, design ID: 042421) for 17 hours at 65°C in a rotating Agilent hybridization oven (Agilent Technologies). .. Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Transformation Assay:

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies). .. The microarray data were submitted to the public repository Gene Expression Omnibus (GEO accession number GSE94338). c) Analysis of microarray expression data Raw data were normalized through intra-array median subtraction and log2 transformation using R statistical software [ ].

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies). .. Raw data were normalized through intra-array median subtraction and log2 transformation, and then, the expression values were analyzed with a mixture model variance (VarMixt) method (R software).

Purification:

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: For each sample (n = 61), a total amount of 100 ng of total RNA was dephosphorylated, 3′ end-labelled with Cy3-pCp, purified on Micro Bio-Spin 6 columns (Bio-Rad, USA), dried, and hybridized to the arrays using the miRNA Complete Labeling and Hybridization Kit (Agilent Technologies) according to the manufacturer’s instructions. .. After a washing step, hybridized microarray slides were scanned using a G2565CA Scanner System (Agilent Technologies) and a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Thermal manipulation of the chicken embryo triggers differential gene expression in response to a later heat challenge
Article Snippet: The cRNA obtained was purified using RNeasy Mini Spin columns (Qiagen) and subsequently run on a Bioanalyzer 2100 using RNA 6000 Nano chips (Agilent Technologies) to assess the profiles. .. The slides were finally scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a 20 bit dynamic range.

Blocking Assay:

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: For each sample, 1.65 μg of Cy3-labeled cRNA (specific activity > 9.0 pmol Cy3/μg of cRNA) were fragmented at 60 °C for 30 minutes in a volume of 55 μl containing 25× Agilent Fragmentation Buffer and 10× Agilent Blocking Agent. .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: For each sample, 600 ng of Cy3-labeled cRNA (specific activity > 6.0 pmol Cy3/μg of cRNA) were fragmented at 60°C for 30 minutes in a reaction volume of 25 μl containing 25× Agilent Fragmentation Buffer and 10× Agilent Blocking Agent, following the manufacturer’s instructions. .. Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits.

Article Title: The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus
Article Snippet: Subsequently, for each sample 600 ng of Cy3-labeled cRNA (specific activity > 9.0 pmol Cy3/µg of cRNA) were fragmented at 60 °C for 30 minutes in a volume of 25 µl containing 25x of Agilent Fragmentation Buffer and 10x of Agilent Blocking Agent. .. The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 µm and a dynamic range of 20 bit.

Software:

Article Title: Interleukin-22 Immunotherapy during Severe Influenza Enhances Lung Tissue Integrity and Reduces Secondary Bacterial Systemic Invasion
Article Snippet: After being washed, the slides were scanned using a G2565CA scanner system (Agilent Technologies), with a resolution of 3 μm and a 20-bit dynamic range. .. The resulting TIFF images were analyzed with Feature Extraction software, version 10.7.3.1 (Agilent Technologies), using a GE1_107_Sep09 protocol.

Article Title: CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands
Article Snippet: After washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies) at a resolution of 3 μm and a dynamic range of 20 bits. .. The resulting TIFF images were analyzed using the Feature Extraction Software v10.7.3.1 (Agilent Technologies) according to the GE1_107_Sep09 protocol.

Article Title: Docosahexaenoic acid mechanisms of action on the bovine oocyte-cumulus complex
Article Snippet: The slides were scanned immediately after washing using a G2565CA Scanner System with one color scan setting for 8 × 60 k array slides (Agilent Technologies). .. The resulting images were analyzed using Feature Extraction Software v10.7.3.1 (Agilent Technologies).

Article Title: Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle
Article Snippet: Slides were washed, air-dried, and scanned immediately using a G2565CA Scanner System with one-color scan setting (Agilent Technologies). .. The resulting images were analyzed using Feature Extraction Software v10.7.3.1 (Agilent Technologies).

Article Title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
Article Snippet: The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits. .. The resulting TIFF images were analysed with the Feature Extraction Software v10.7.3.1 (Agilent Technologies), using the GE1_107_Sep09 protocol.

Article Title: Thermal manipulation of the chicken embryo triggers differential gene expression in response to a later heat challenge
Article Snippet: The slides were finally scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 μm and a 20 bit dynamic range. .. The resulting .tiff images were analysed with Feature Extraction Software (Agilent Technologies) using the GE2_107_Sep09 protocol to obtain background-subtracted and spatially-detrended processed signal intensities.

Article Title: Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin
Article Snippet: Immediately after washing, the slides were scanned using a G2565CA Scanner System (Agilent Technologies), which used a scan protocol with a resolution of 3 μm and a dynamic range of 20 bits. .. The resulting .tiff images were analyzed with the Feature Extraction Software v10.7.3.1 (Agilent Technologies), using the GE2_107_Sep09 protocol.

Article Title: The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus
Article Snippet: The slides were then scanned using a G2565CA Scanner System (Agilent Technologies), using a scan protocol with a resolution of 3 µm and a dynamic range of 20 bit. .. The resulting.tiff images were analyzed with the Feature Extraction Software v10.7.3.1 (Agilent Technologies), using the GE1_107_Sep09 protocol.

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  • 99
    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g2565ca dna microarray scanner/product/Agilent technologies
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    g2565ca dna microarray scanner - by Bioz Stars, 2020-04
    93/100 stars
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    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Principle of multiplex SOMAmer affinity assay. (A) Binding. SOMAmers and samples are mixed in 96-well microwell plates and allowed to bind. Cognate and non-cognate SOMAmer-target protein complexes form. Free SOMAmer and protein are also present. (B–H) Schematic sequence of assay steps leading to quantitative readout of target proteins. (B) SOMAmer-protein binding: DNA-based SOMAmer molecules (gold, blue, and green) have unique shapes selected to bind to a specific protein. SOMAmers contain biotin (B), a photo-cleavable linker (L) and a fluorescent tag at the 5′ end. Most SOMAmers (gold and green) bind to cognate proteins (red), but some (blue) form non-cognate complexes. (C) Catch-1. SOMAmers are captured onto a bead coated with streptavidin (SA) which binds biotin. Un-complexed proteins are washed away. (D) Proteins are tagged with NHS-biotin. (E) Photocleavage and kinetic challenge. UV light (hν) cleaves the linker and SOMAmers are released from beads, leaving biotin on bead. Samples are challenged with anionic competitor (dextran sulfate). Non-cognate complexes (blue SOMAmer) preferentially dissociate. (F) Catch-2 SOMAmer-protein complexes are captured onto new avidin coated beads by protein biotin tag. Free SOMAmers are washed away. (G) SOMAmers are released from complexes into solution at high pH. (H) Remaining SOMAmers are quantified by hybridization to microarray containing single-stranded DNA probes complementary to SOMAmer DNA sequence, which form a double-stranded helix. Hybridized SOMAmers are detected by fluorescent tags when the array is scanned.

    Journal: PLoS ONE

    Article Title: Aptamer-Based Multiplexed Proteomic Technology for Biomarker DiscoveryUnlocking Biomarker Discovery: Large Scale Application of Aptamer Proteomic Technology for Early Detection of Lung Cancer

    doi: 10.1371/journal.pone.0015004

    Figure Lengend Snippet: Principle of multiplex SOMAmer affinity assay. (A) Binding. SOMAmers and samples are mixed in 96-well microwell plates and allowed to bind. Cognate and non-cognate SOMAmer-target protein complexes form. Free SOMAmer and protein are also present. (B–H) Schematic sequence of assay steps leading to quantitative readout of target proteins. (B) SOMAmer-protein binding: DNA-based SOMAmer molecules (gold, blue, and green) have unique shapes selected to bind to a specific protein. SOMAmers contain biotin (B), a photo-cleavable linker (L) and a fluorescent tag at the 5′ end. Most SOMAmers (gold and green) bind to cognate proteins (red), but some (blue) form non-cognate complexes. (C) Catch-1. SOMAmers are captured onto a bead coated with streptavidin (SA) which binds biotin. Un-complexed proteins are washed away. (D) Proteins are tagged with NHS-biotin. (E) Photocleavage and kinetic challenge. UV light (hν) cleaves the linker and SOMAmers are released from beads, leaving biotin on bead. Samples are challenged with anionic competitor (dextran sulfate). Non-cognate complexes (blue SOMAmer) preferentially dissociate. (F) Catch-2 SOMAmer-protein complexes are captured onto new avidin coated beads by protein biotin tag. Free SOMAmers are washed away. (G) SOMAmers are released from complexes into solution at high pH. (H) Remaining SOMAmers are quantified by hybridization to microarray containing single-stranded DNA probes complementary to SOMAmer DNA sequence, which form a double-stranded helix. Hybridized SOMAmers are detected by fluorescent tags when the array is scanned.

    Article Snippet: Microarray Imaging The microarray slides were imaged with a microarray scanner (Agilent G2565CA Microarray Scanner System, Agilent Technologies) in the Cy3-channel at 5 µm resolution at 100% PMT setting and the XRD option enabled at 0.05.

    Techniques: Multiplex Assay, Binding Assay, Sequencing, Protein Binding, Avidin-Biotin Assay, Hybridization, Microarray

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization