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Agilent technologies g2565ca dna scanner
G2565ca Dna Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g2565ca dna scanner - by Bioz Stars, 2020-04
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Expressing:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Arabidopsis (V4) gene expression microarrays covering 4 × 44 K probes (Agilent microarray design ID 021169, P/N G2519F-021169) were used for subsequent hybridisations in a quadruplicate design in which each exposure condition was labelled in one colour and compared with the unexposed control in the other colour. .. Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Homogenization:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Plants were collected from the test tubes and put on 3MM Whatman paper to remove excess MS and then frozen in liquid nitrogen prior to homogenization, as described before [ ]. .. Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Isolation:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Total RNA was extracted with TriPure Isolation Reagent (Roche, Penzberg, Germany) and quantified using a NanoDrop ND-1000 UV–VIS spectrophotometer (Wilmington, DE, USA). .. Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Labeling:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: A total of 100 ng of nucleic acid from each replicate were retrotranscribed (first strand synthesis) and labelled using a Low Input Quick Amp Labeling kit (Agilent) following manufacturer protocols for two-colour, microarray-based, gene expression analysis. .. Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Produced:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent). .. Normalised microarray data from the 43,663 oligo probes that produced detectable signals were reduced to 26,184 gene entities representing the Gene Level experiment that was studied with GeneSpring GX.

Microarray:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Paragraph title: Microarray hybridisation and transcriptomic analysis ... Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Spectrophotometry:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Total RNA was extracted with TriPure Isolation Reagent (Roche, Penzberg, Germany) and quantified using a NanoDrop ND-1000 UV–VIS spectrophotometer (Wilmington, DE, USA). .. Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Mass Spectrometry:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Plants were collected from the test tubes and put on 3MM Whatman paper to remove excess MS and then frozen in liquid nitrogen prior to homogenization, as described before [ ]. .. Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Hybridization:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Paragraph title: Microarray hybridisation and transcriptomic analysis ... Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent).

Software:

Article Title: Early response to nanoparticles in the Arabidopsis transcriptome compromises plant defence and root-hair development through salicylic acid signalling
Article Snippet: Hybridised microarrays were scanned on a G2565CA DNA scanner (Agilent). .. Microarray hybridisation and feature extraction, as well as RNA quality report and labelling, were performed according to Agilent standard procedures and software using the Gene Expression Unit of the Genomics and Proteomics Facility in the UPV/EHU (SGIker platforms; Leioa, Spain).

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  • 99
    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g2565ca dna microarray scanner/product/Agilent technologies
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    g2565ca dna microarray scanner - by Bioz Stars, 2020-04
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    94
    Agilent technologies dna microarray scanner g2565ba
    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through <t>DNA</t> <t>microarray</t> analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
    Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner g2565ba/product/Agilent technologies
    Average 94 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner g2565ba - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization

    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: RNA Expression, Microarray, Expressing

    Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: Expressing, Microarray