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Agilent technologies g2565ba microarray scanner system
G2565ba Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g2565ba microarray scanner system - by Bioz Stars, 2020-03
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Related Articles

RNA Extraction:

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: Microarray analysis The mirVana miRNA Isolation kit (Ambion, Austin, TX, USA) was used for total RNA extraction according to the manufacturer's protocol. .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition.

Incubation:

Article Title: Suppression of Adipogenesis by Pathogenic Seipin Mutant Is Associated with Inflammatory Response
Article Snippet: The synthesis of the fluorescent-labelled cRNA was performed during the second incubation step (2 h at 40°C). .. The slides were scanned with the Agilent G2565BA Microarray Scanner System.

Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
Article Snippet: DNA Microarray HPB-ALL cells were nucleofected with control siRNA, siRNA1, siRNA3-1, or siRNA3-2, and incubated for 48 h. Total RNA was then prepared and labeled with Cy3. .. Arrays were scanned with a G2565BA Microarray Scanner System (Agilent), and the resulting data were analyzed using the GeneSpring GX software (Agilent).

Synthesized:

Article Title: Suppression of Adipogenesis by Pathogenic Seipin Mutant Is Associated with Inflammatory Response
Article Snippet: Briefly, cDNA was synthesized for 2 hr at 40°C followed by denaturation for 10 min at 65°C. .. The slides were scanned with the Agilent G2565BA Microarray Scanner System.

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: Complementary DNA (cDNA) and biotinylated cRNA were synthesized and hybridized to the array. .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition.

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: Aminoallyl-cRNA was synthesized from cDNA using the Superscript Indirect cDNA Labeling System (Agilent, Palo Alto, CA, USA). .. The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology.

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: The integrity of total RNA was checked using an Agilent 2100 Bioanalyzer (Agilent). cDNA and biotinylated cRNA were synthesized and hybridized to the array. .. Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1).

Multiple Displacement Amplification:

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: Total RNA was isolated from MDA-MB-231 cells after transfection with DDX5 siRNA, control siRNA, and negative control (transfection mix only), respectively, using the miRCURYTM RNA isolation kit. .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.).

Isolation:

Article Title: Integration of microRNAome, proteomics and metabolomics to analyze arsenic-induced malignant cell transformation
Article Snippet: The miRCURY™ RNA Isolation Kit and the miRCURY™ LNA Array power labeling kit (Exiqon) were used to extract total RNA and label the samples. .. The samples were subsequently scanned using the Agilent G2565BA Microarray Scanner System (Santa Clara, CA).

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: Microarray analysis The mirVana miRNA Isolation kit (Ambion, Austin, TX, USA) was used for total RNA extraction according to the manufacturer's protocol. .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition.

Article Title: microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)
Article Snippet: Paragraph title: RNA isolation and miRNA microarray assay ... Images were then scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA, USA).

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: Total RNA was isolated using Trizol and purified with the miRNeasy mini kit (Qiagen). miRNA expression profiling including labeling, hybridization, scanning, normalization, and data analysis was performed at Exiqon. .. Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery).

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: Total RNA was isolated from MDA-MB-231 cells after transfection with DDX5 siRNA, control siRNA, and negative control (transfection mix only), respectively, using the miRCURYTM RNA isolation kit. .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.).

Software:

Article Title: Suppression of Adipogenesis by Pathogenic Seipin Mutant Is Associated with Inflammatory Response
Article Snippet: The slides were scanned with the Agilent G2565BA Microarray Scanner System. .. The Agilent G2567AA Feature Extraction Software v.9.1 (Agilent Technologies UK) was used for data extraction and quality control.

Article Title: Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276
Article Snippet: .. The miRCURY LNA™microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon). .. The data obtained were subjected to statistical analysis and differentially regulated miRNAs in infected and uninfected cells were reported.

Article Title: Therapeutic Action of Fluoxetine is Associated with a Reduction in Prefrontal Cortical miR-1971 Expression Levels in a Mouse Model of Posttraumatic Stress Disorder
Article Snippet: The miRCURY LNA™ miRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA). .. Image analysis was carried out with ImaGene® 9 miRCURY LNA™ miRNA Array Analysis Software (Exiqon A/S, Vedbaek, Denmark).

Article Title: Integration of microRNAome, proteomics and metabolomics to analyze arsenic-induced malignant cell transformation
Article Snippet: The samples were subsequently scanned using the Agilent G2565BA Microarray Scanner System (Santa Clara, CA). .. ImaGene 8.0 software was used for the image analysis (BioDiscovery, Inc., Hawthorne, CA).

Article Title: MicroRNA Signatures in Tumor Tissue Related to Angiogenesis in Non-Small Cell Lung Cancer
Article Snippet: .. The miCURY™ LNA Array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent technologies Inc., USA) and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc., USA). ..

Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
Article Snippet: .. Arrays were scanned with a G2565BA Microarray Scanner System (Agilent), and the resulting data were analyzed using the GeneSpring GX software (Agilent). .. The microarray analysis to monitor human non-protein coding transcripts was conducted as described previously [ ].

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition. .. Probe intensities were normalized using GeneSpring GX 11.0 (Agilent, Palo Alto, CA, USA).

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology. .. The TIFF image generated was loaded into Feature Extraction Software (Agilent Technologies, Sisha Clara, CA, USA) for feature data extraction, and data analysis was performed with MultiExperiment Viewer (MeV) (Boston, MA, USA).

Article Title: Assessment of a novel multi-array normalization method based on spike-in control probes suitable for microRNA datasets with global decreases in expression
Article Snippet: .. Following hybridization and processing, the arrays were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and image analysis was performed using the ImaGene 8.0 software (BioDiscovery, Inc., Santa Clara, CA) to quantify the signals. .. The raw data were provided by Exiqon as ImaGene text files.

Article Title: microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)
Article Snippet: Images were then scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA, USA). .. Results were analyzed using ImaGene™ software (BioDiscovery, CA, USA).

Article Title: MicroRNA-210 Decreases heme Levels by Targeting Ferrochelatase in Cardiomyocytes
Article Snippet: .. The miRCURY LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc.) and the image analysis was carried out using the ImaGene 9.0 software (BioDiscovery, Inc.). ..

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: .. Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery). ..

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: .. Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1). ..

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.). ..

Article Title: MicroRNA-Mediated Suppression of the TGF-β Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance
Article Snippet: .. The miRCURY LNA microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies) and image analysis was carried out using the ImaGene® 9 (miRCURY LNA microRNA Array Analysis Software, Exiqon). ..

Negative Control:

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: Total RNA was isolated from MDA-MB-231 cells after transfection with DDX5 siRNA, control siRNA, and negative control (transfection mix only), respectively, using the miRCURYTM RNA isolation kit. .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.).

Labeling:

Article Title: Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276
Article Snippet: RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon) following the procedure described by the manufacturer. .. The miRCURY LNA™microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon).

Article Title: Therapeutic Action of Fluoxetine is Associated with a Reduction in Prefrontal Cortical miR-1971 Expression Levels in a Mouse Model of Posttraumatic Stress Disorder
Article Snippet: The Hy3™ -labeled samples were mixed pairwise with a Hy5™ -labeled reference probe and hybridized to the miRCURY LNA™ miRNA Array 7th Gen (Exiqon A/S, Vedbaek, Denmark) which contains capture probes targeting all miRNAs registered in the miRBase 18.0 (human, mouse, or rat) as well as viral miRNAs related to these species. .. The miRCURY LNA™ miRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA).

Article Title: Integration of microRNAome, proteomics and metabolomics to analyze arsenic-induced malignant cell transformation
Article Snippet: The miRCURY™ RNA Isolation Kit and the miRCURY™ LNA Array power labeling kit (Exiqon) were used to extract total RNA and label the samples. .. The samples were subsequently scanned using the Agilent G2565BA Microarray Scanner System (Santa Clara, CA).

Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
Article Snippet: DNA Microarray HPB-ALL cells were nucleofected with control siRNA, siRNA1, siRNA3-1, or siRNA3-2, and incubated for 48 h. Total RNA was then prepared and labeled with Cy3. .. Arrays were scanned with a G2565BA Microarray Scanner System (Agilent), and the resulting data were analyzed using the GeneSpring GX software (Agilent).

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: Labeling and hybridization of the cRNA was performed with Agilent Whole Porcine Genome Oligo (4 × 44K) Microarrays (one-color platform) at the National Engineering Center for Biochip at Shanghai, according to the manufacturer’s protocols. .. The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology.

Article Title: Assessment of a novel multi-array normalization method based on spike-in control probes suitable for microRNA datasets with global decreases in expression
Article Snippet: The spike-in controls “a” , “b” , “c” , “d” , “e” , “f” , “g” , “h” , “i ”, and “j” were spiked into the labeling reaction at various concentrations. .. Following hybridization and processing, the arrays were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and image analysis was performed using the ImaGene 8.0 software (BioDiscovery, Inc., Santa Clara, CA) to quantify the signals.

Article Title: microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)
Article Snippet: RNA samples were labeled using the miRCURY Hy3/Hy5 Power labeling kit (Exiqon Inc., Woburn, MA, USA) and hybridized on the miRCURY LNA Array station. .. Images were then scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA, USA).

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: One μ g total RNA of each sample and a common reference pool were labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA Array power labeling kit (Exiqon). .. Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery).

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: Total RNA from the three individual samples and reference (common reference pool of the three samples) was labeled with Hy3™ and Hy5™ fluorescent labels, respectively, using the miRCURY™ LNA Array power labeling kit (Exiqon). .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.).

Article Title: MicroRNA-Mediated Suppression of the TGF-β Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance
Article Snippet: miRNA Profiling For miRNA arrays, 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA microRNA Hi-Power Labeling Kit, Hy3/Hy5 (Exiqon) following the procedure described by the manufacturer. .. The miRCURY LNA microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies) and image analysis was carried out using the ImaGene® 9 (miRCURY LNA microRNA Array Analysis Software, Exiqon).

Purification:

Article Title: Suppression of Adipogenesis by Pathogenic Seipin Mutant Is Associated with Inflammatory Response
Article Snippet: The labelled cRNA was purified with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. .. The slides were scanned with the Agilent G2565BA Microarray Scanner System.

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: The cRNA was purified and DNase treated using RNeasy QIAGEN RNeasy® Mini Kit (QIAGEN, Hilden, Germany). .. The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology.

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: Total RNA was isolated using Trizol and purified with the miRNeasy mini kit (Qiagen). miRNA expression profiling including labeling, hybridization, scanning, normalization, and data analysis was performed at Exiqon. .. Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery).

Electrophoresis:

Article Title: microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)
Article Snippet: RNA quality and quantity were determined via standard electrophoresis and spectrophotometric methods. .. Images were then scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA, USA).

Microarray:

Article Title: Suppression of Adipogenesis by Pathogenic Seipin Mutant Is Associated with Inflammatory Response
Article Snippet: .. The slides were scanned with the Agilent G2565BA Microarray Scanner System. .. The Agilent G2567AA Feature Extraction Software v.9.1 (Agilent Technologies UK) was used for data extraction and quality control.

Article Title: Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276
Article Snippet: .. The miRCURY LNA™microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon). .. The data obtained were subjected to statistical analysis and differentially regulated miRNAs in infected and uninfected cells were reported.

Article Title: Therapeutic Action of Fluoxetine is Associated with a Reduction in Prefrontal Cortical miR-1971 Expression Levels in a Mouse Model of Posttraumatic Stress Disorder
Article Snippet: .. The miRCURY LNA™ miRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA). .. Image analysis was carried out with ImaGene® 9 miRCURY LNA™ miRNA Array Analysis Software (Exiqon A/S, Vedbaek, Denmark).

Article Title: Integration of microRNAome, proteomics and metabolomics to analyze arsenic-induced malignant cell transformation
Article Snippet: .. The samples were subsequently scanned using the Agilent G2565BA Microarray Scanner System (Santa Clara, CA). .. ImaGene 8.0 software was used for the image analysis (BioDiscovery, Inc., Hawthorne, CA).

Article Title: MicroRNA Signatures in Tumor Tissue Related to Angiogenesis in Non-Small Cell Lung Cancer
Article Snippet: .. The miCURY™ LNA Array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent technologies Inc., USA) and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc., USA). ..

Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
Article Snippet: .. Arrays were scanned with a G2565BA Microarray Scanner System (Agilent), and the resulting data were analyzed using the GeneSpring GX software (Agilent). .. The microarray analysis to monitor human non-protein coding transcripts was conducted as described previously [ ].

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition. .. Probe intensities were normalized using GeneSpring GX 11.0 (Agilent, Palo Alto, CA, USA).

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: .. The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology. .. The TIFF image generated was loaded into Feature Extraction Software (Agilent Technologies, Sisha Clara, CA, USA) for feature data extraction, and data analysis was performed with MultiExperiment Viewer (MeV) (Boston, MA, USA).

Article Title: Assessment of a novel multi-array normalization method based on spike-in control probes suitable for microRNA datasets with global decreases in expression
Article Snippet: .. Following hybridization and processing, the arrays were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and image analysis was performed using the ImaGene 8.0 software (BioDiscovery, Inc., Santa Clara, CA) to quantify the signals. .. The raw data were provided by Exiqon as ImaGene text files.

Article Title: microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)
Article Snippet: .. Images were then scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA, USA). .. Results were analyzed using ImaGene™ software (BioDiscovery, CA, USA).

Article Title: MicroRNA-210 Decreases heme Levels by Targeting Ferrochelatase in Cardiomyocytes
Article Snippet: .. The miRCURY LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc.) and the image analysis was carried out using the ImaGene 9.0 software (BioDiscovery, Inc.). ..

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: .. Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery). ..

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: .. Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1). ..

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.). ..

Article Title: MicroRNA-Mediated Suppression of the TGF-β Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance
Article Snippet: .. The miRCURY LNA microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies) and image analysis was carried out using the ImaGene® 9 (miRCURY LNA microRNA Array Analysis Software, Exiqon). ..

Generated:

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology. .. The TIFF image generated was loaded into Feature Extraction Software (Agilent Technologies, Sisha Clara, CA, USA) for feature data extraction, and data analysis was performed with MultiExperiment Viewer (MeV) (Boston, MA, USA).

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: Besides, the data we generated can be retrieved partly on the public platforms Chinese Glioma Genome Atlas (CGGA) ( http://www.cgga.org.cn ). .. Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1).

Transfection:

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: Total RNA was isolated from MDA-MB-231 cells after transfection with DDX5 siRNA, control siRNA, and negative control (transfection mix only), respectively, using the miRCURYTM RNA isolation kit. .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.).

Infection:

Article Title: Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276
Article Snippet: The miRCURY LNA™microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon). .. The data obtained were subjected to statistical analysis and differentially regulated miRNAs in infected and uninfected cells were reported.

Expressing:

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology. .. The array data has been submitted to gene expression omnibus (GEO) GSE42317 [ ].

Article Title: microRNA-204 modulates chemosensitivity and apoptosis of prostate cancer cells by targeting zinc-finger E-box-binding homeobox 1 (ZEB1)
Article Snippet: Samples were randomly selected from different treatment groups. miRNA expression analysis was performed using miRCURY LNA (Locked Nucleic Acid) microRNA Arrays (Exiqon, A/S, Vedbaek, Denmark) version 10.0. .. Images were then scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA, USA).

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: Paragraph title: Global miRNA Expression Profiling ... Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery).

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: Paragraph title: Whole transcriptome sequencing and whole-genome expression profiling ... Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1).

Genome Wide:

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: In CGGA genome-wide transcriptome microarray data, microarray analysis was performed on all samples using the Agilent Whole Human Genome Array according to the manufacturer’s instructions. .. Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1).

Sequencing:

Article Title: ALDH1A3: A Marker of Mesenchymal Phenotype in Gliomas Associated with Cell Invasion
Article Snippet: Paragraph title: Whole transcriptome sequencing and whole-genome expression profiling ... Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1).

Transformation Assay:

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery). .. Log2 transformed median Hy3 (sample)/Hy5 (common reference pool) ratios were calculated for each capture probe (present in four replicates on the array).

Spectrophotometry:

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: RNA concentration and quality were measured with the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Waltham, MA, USA). .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition.

Concentration Assay:

Article Title: Phosphorylated SATB1 is associated with the progression and prognosis of glioma
Article Snippet: RNA concentration and quality were measured with the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Waltham, MA, USA). .. The Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (version 9.1) were used for data acquisition.

Hybridization:

Article Title: Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276
Article Snippet: After hybridization, the microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) in order to prevent potential bleaching of the fluorescent dyes. .. The miRCURY LNA™microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon).

Article Title: Therapeutic Action of Fluoxetine is Associated with a Reduction in Prefrontal Cortical miR-1971 Expression Levels in a Mouse Model of Posttraumatic Stress Disorder
Article Snippet: After hybridization, microarray slides were scanned and stored in an ozone free environment (ozone below 2.0 ppb) in order to prevent potential bleaching of fluorescent labels. .. The miRCURY LNA™ miRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA).

Article Title: Integration of microRNAome, proteomics and metabolomics to analyze arsenic-induced malignant cell transformation
Article Snippet: Microarray analysis Three control samples and three experimental samples were sent to the LC-Bio Company (Beijing, China) for miRNA microarray hybridization and analysis. .. The samples were subsequently scanned using the Agilent G2565BA Microarray Scanner System (Santa Clara, CA).

Article Title: MicroRNA Signatures in Tumor Tissue Related to Angiogenesis in Non-Small Cell Lung Cancer
Article Snippet: After hybridization the microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) in order to prevent potential bleaching of the fluorescent dyes. .. The miCURY™ LNA Array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent technologies Inc., USA) and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc., USA).

Article Title: Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
Article Snippet: Labeling and hybridization of the cRNA was performed with Agilent Whole Porcine Genome Oligo (4 × 44K) Microarrays (one-color platform) at the National Engineering Center for Biochip at Shanghai, according to the manufacturer’s protocols. .. The slides were scanned and analyzed using the histogram method with default settings in an Agilent G2565AA and Agilent G2565BA Microarray Scanner System using SureScan Technology.

Article Title: Assessment of a novel multi-array normalization method based on spike-in control probes suitable for microRNA datasets with global decreases in expression
Article Snippet: .. Following hybridization and processing, the arrays were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., Santa Clara, CA) and image analysis was performed using the ImaGene 8.0 software (BioDiscovery, Inc., Santa Clara, CA) to quantify the signals. .. The raw data were provided by Exiqon as ImaGene text files.

Article Title: MicroRNA-210 Decreases heme Levels by Targeting Ferrochelatase in Cardiomyocytes
Article Snippet: After hybridization the microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) in order to prevent potential bleaching of the fluorescent dyes. .. The miRCURY LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc.) and the image analysis was carried out using the ImaGene 9.0 software (BioDiscovery, Inc.).

Article Title: MicroRNAs Control Hepatocyte Proliferation During Liver Regeneration
Article Snippet: Hybridization was performed using a Tecan HS4800 hybridization station. .. Slides were scanned using an Agilent G2565BA Microarray Scanner System and image analysis was carried out with ImaGene 7.0 software (Bio-Discovery).

Article Title: RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells *
Article Snippet: The hybridization was performed according to the miRCURY™ LNA array manual using a Tecan HS4800 hybridization station (Tecan, Austria). .. The microarray slides were scanned using the Agilent G2565BA Microarray Scanner System and the image analysis was carried out using the ImaGene 8.0 software (BioDiscovery Inc.).

Article Title: MicroRNA-Mediated Suppression of the TGF-β Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance
Article Snippet: After hybridization, the microarray slides were scanned and stored in an ozone-free environment (ozone level below 2.0 ppb) in order to prevent potential bleaching of the fluorescent dyes. .. The miRCURY LNA microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies) and image analysis was carried out using the ImaGene® 9 (miRCURY LNA microRNA Array Analysis Software, Exiqon).

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    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Principle of multiplex SOMAmer affinity assay. (A) Binding. SOMAmers and samples are mixed in 96-well microwell plates and allowed to bind. Cognate and non-cognate SOMAmer-target protein complexes form. Free SOMAmer and protein are also present. (B–H) Schematic sequence of assay steps leading to quantitative readout of target proteins. (B) SOMAmer-protein binding: DNA-based SOMAmer molecules (gold, blue, and green) have unique shapes selected to bind to a specific protein. SOMAmers contain biotin (B), a photo-cleavable linker (L) and a fluorescent tag at the 5′ end. Most SOMAmers (gold and green) bind to cognate proteins (red), but some (blue) form non-cognate complexes. (C) Catch-1. SOMAmers are captured onto a bead coated with streptavidin (SA) which binds biotin. Un-complexed proteins are washed away. (D) Proteins are tagged with NHS-biotin. (E) Photocleavage and kinetic challenge. UV light (hν) cleaves the linker and SOMAmers are released from beads, leaving biotin on bead. Samples are challenged with anionic competitor (dextran sulfate). Non-cognate complexes (blue SOMAmer) preferentially dissociate. (F) Catch-2 SOMAmer-protein complexes are captured onto new avidin coated beads by protein biotin tag. Free SOMAmers are washed away. (G) SOMAmers are released from complexes into solution at high pH. (H) Remaining SOMAmers are quantified by hybridization to microarray containing single-stranded DNA probes complementary to SOMAmer DNA sequence, which form a double-stranded helix. Hybridized SOMAmers are detected by fluorescent tags when the array is scanned.

    Journal: PLoS ONE

    Article Title: Aptamer-Based Multiplexed Proteomic Technology for Biomarker DiscoveryUnlocking Biomarker Discovery: Large Scale Application of Aptamer Proteomic Technology for Early Detection of Lung Cancer

    doi: 10.1371/journal.pone.0015004

    Figure Lengend Snippet: Principle of multiplex SOMAmer affinity assay. (A) Binding. SOMAmers and samples are mixed in 96-well microwell plates and allowed to bind. Cognate and non-cognate SOMAmer-target protein complexes form. Free SOMAmer and protein are also present. (B–H) Schematic sequence of assay steps leading to quantitative readout of target proteins. (B) SOMAmer-protein binding: DNA-based SOMAmer molecules (gold, blue, and green) have unique shapes selected to bind to a specific protein. SOMAmers contain biotin (B), a photo-cleavable linker (L) and a fluorescent tag at the 5′ end. Most SOMAmers (gold and green) bind to cognate proteins (red), but some (blue) form non-cognate complexes. (C) Catch-1. SOMAmers are captured onto a bead coated with streptavidin (SA) which binds biotin. Un-complexed proteins are washed away. (D) Proteins are tagged with NHS-biotin. (E) Photocleavage and kinetic challenge. UV light (hν) cleaves the linker and SOMAmers are released from beads, leaving biotin on bead. Samples are challenged with anionic competitor (dextran sulfate). Non-cognate complexes (blue SOMAmer) preferentially dissociate. (F) Catch-2 SOMAmer-protein complexes are captured onto new avidin coated beads by protein biotin tag. Free SOMAmers are washed away. (G) SOMAmers are released from complexes into solution at high pH. (H) Remaining SOMAmers are quantified by hybridization to microarray containing single-stranded DNA probes complementary to SOMAmer DNA sequence, which form a double-stranded helix. Hybridized SOMAmers are detected by fluorescent tags when the array is scanned.

    Article Snippet: Microarray Imaging The microarray slides were imaged with a microarray scanner (Agilent G2565CA Microarray Scanner System, Agilent Technologies) in the Cy3-channel at 5 µm resolution at 100% PMT setting and the XRD option enabled at 0.05.

    Techniques: Multiplex Assay, Binding Assay, Sequencing, Protein Binding, Avidin-Biotin Assay, Hybridization, Microarray

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization