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Agilent technologies g2565aa scanner
G2565aa Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/g2565aa scanner/product/Agilent technologies
Average 78 stars, based on 1 article reviews
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g2565aa scanner - by Bioz Stars, 2020-01
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Microarray:

Article Title: Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori
Article Snippet: Paragraph title: Microarray experiments. ... Fluorescent signal intensities were measured with a G2565AA scanner (Agilent Technologies, Palo Alto, California, United States), and feature extraction was performed with ImaGene (Biodiscovery, El Segundo, California, United States).

Labeling:

Article Title: Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori
Article Snippet: 2 μg of purified labeled DNA from each test strain was mixed with 1 μg each from 26695 and J99 that had been labeled with the alternate dye and hybridized in DIG Easy Hyb buffer (Roche, Basel, Switzerland) to a microarray slide for 15–18 h at 37 °C. .. Fluorescent signal intensities were measured with a G2565AA scanner (Agilent Technologies, Palo Alto, California, United States), and feature extraction was performed with ImaGene (Biodiscovery, El Segundo, California, United States).

Purification:

Article Title: Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori
Article Snippet: 2 μg of purified labeled DNA from each test strain was mixed with 1 μg each from 26695 and J99 that had been labeled with the alternate dye and hybridized in DIG Easy Hyb buffer (Roche, Basel, Switzerland) to a microarray slide for 15–18 h at 37 °C. .. Fluorescent signal intensities were measured with a G2565AA scanner (Agilent Technologies, Palo Alto, California, United States), and feature extraction was performed with ImaGene (Biodiscovery, El Segundo, California, United States).

Concentration Assay:

Article Title: Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori
Article Snippet: DIG Easy Hyb buffer contains urea in a concentration that results in hybridization conditions that are comparable to 50% formamide content. .. Fluorescent signal intensities were measured with a G2565AA scanner (Agilent Technologies, Palo Alto, California, United States), and feature extraction was performed with ImaGene (Biodiscovery, El Segundo, California, United States).

Hybridization:

Article Title: Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori
Article Snippet: DIG Easy Hyb buffer contains urea in a concentration that results in hybridization conditions that are comparable to 50% formamide content. .. Fluorescent signal intensities were measured with a G2565AA scanner (Agilent Technologies, Palo Alto, California, United States), and feature extraction was performed with ImaGene (Biodiscovery, El Segundo, California, United States).

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    Agilent technologies high resolution microarray scanner
    BDNF induces the dissociation of mRNA from hnRNP K at the synapse. A , B , Effect of BDNF stimulation (50 ng/ml; 10 min) on transcript coimmunoprecipitation with hnRNP K in cultured hippocampal neurons as assessed by <t>microarray</t> and qPCR. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. A , Genes coding for synaptic proteins: GluA1, AMPA receptor subunit 1; GluA2, AMPA receptor subunit 2; GluN1, NMDAR subunit 1. B , Other genes: TrkB, tropomyosin-related kinase B receptor; NPAS4, neuronal PAS domain protein 4. The results represent quantitation of four different experiments performed in independent preparations, and are expressed as percentage [mean ± SEM (qPCR) or SD (microarray)] of control; * p
    High Resolution Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution microarray scanner/product/Agilent technologies
    Average 99 stars, based on 302 article reviews
    Price from $9.99 to $1999.99
    high resolution microarray scanner - by Bioz Stars, 2020-01
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    BDNF induces the dissociation of mRNA from hnRNP K at the synapse. A , B , Effect of BDNF stimulation (50 ng/ml; 10 min) on transcript coimmunoprecipitation with hnRNP K in cultured hippocampal neurons as assessed by microarray and qPCR. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. A , Genes coding for synaptic proteins: GluA1, AMPA receptor subunit 1; GluA2, AMPA receptor subunit 2; GluN1, NMDAR subunit 1. B , Other genes: TrkB, tropomyosin-related kinase B receptor; NPAS4, neuronal PAS domain protein 4. The results represent quantitation of four different experiments performed in independent preparations, and are expressed as percentage [mean ± SEM (qPCR) or SD (microarray)] of control; * p

    Journal: eNeuro

    Article Title: The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons

    doi: 10.1523/ENEURO.0268-17.2017

    Figure Lengend Snippet: BDNF induces the dissociation of mRNA from hnRNP K at the synapse. A , B , Effect of BDNF stimulation (50 ng/ml; 10 min) on transcript coimmunoprecipitation with hnRNP K in cultured hippocampal neurons as assessed by microarray and qPCR. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. A , Genes coding for synaptic proteins: GluA1, AMPA receptor subunit 1; GluA2, AMPA receptor subunit 2; GluN1, NMDAR subunit 1. B , Other genes: TrkB, tropomyosin-related kinase B receptor; NPAS4, neuronal PAS domain protein 4. The results represent quantitation of four different experiments performed in independent preparations, and are expressed as percentage [mean ± SEM (qPCR) or SD (microarray)] of control; * p

    Article Snippet: The Whole Rat Genome Microarray kit (4 × 44K, Agilent Technologies) was used and analyzed with a high-resolution microarray scanner (G2565AA, Agilent Technologies). hnRNP K-bound mRNAs were identified by setting a cutoff value of the fold variation between the hnRNP K and IgG samples.

    Techniques: Cell Culture, Microarray, Real-time Polymerase Chain Reaction, Quantitation Assay

    Stimulation of hippocampal neurons with BDNF decreases the interaction of hnRNP K with a large number of transcripts. A , B , List of the 10 most significantly enriched biological processes associated with mRNAs bound to hnRNP K ( A ) and those that were regulated by BDNF (50 ng/ml for 10 min) ( B ), identified with the PANTHER classification system. Only categories showing at least a 2-fold enrichment (considering the size of our lists) were analyzed and the 10 categories displaying the highest –log 10 ( p value) are shown. The number of transcripts belonging to each category and the fold change (designated in parenthesis) are indicated within graph bars. reg., regulation; pres., presynaptic; chem, chemical; syn., synaptic; pos., positive; proj., projection; diff., differentiation; neg., negative; sys., system; devel., development. C , Cultured hippocampal neurons were stimulated or not with BDNF (50 ng/ml) for 10 min before preparation of cellular extracts. hnRNP K was immunoprecipitated from control and BDNF-treated hippocampal neuron homogenates, and the associated transcripts were identified by microarray analysis. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. The results were obtained from the quantification of four different experiments performed in independent preparations, and are expressed as -log ( p value) and log fold change (BDNF vs control). A total of 9509 transcripts showed a decrease in the interaction with hnRNP K in cells stimulated with BDNF; p

    Journal: eNeuro

    Article Title: The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons

    doi: 10.1523/ENEURO.0268-17.2017

    Figure Lengend Snippet: Stimulation of hippocampal neurons with BDNF decreases the interaction of hnRNP K with a large number of transcripts. A , B , List of the 10 most significantly enriched biological processes associated with mRNAs bound to hnRNP K ( A ) and those that were regulated by BDNF (50 ng/ml for 10 min) ( B ), identified with the PANTHER classification system. Only categories showing at least a 2-fold enrichment (considering the size of our lists) were analyzed and the 10 categories displaying the highest –log 10 ( p value) are shown. The number of transcripts belonging to each category and the fold change (designated in parenthesis) are indicated within graph bars. reg., regulation; pres., presynaptic; chem, chemical; syn., synaptic; pos., positive; proj., projection; diff., differentiation; neg., negative; sys., system; devel., development. C , Cultured hippocampal neurons were stimulated or not with BDNF (50 ng/ml) for 10 min before preparation of cellular extracts. hnRNP K was immunoprecipitated from control and BDNF-treated hippocampal neuron homogenates, and the associated transcripts were identified by microarray analysis. The specificity of transcripts associated with hnRNP K was assessed by subtracting the levels of correspondent mRNAs pulled down together with mouse IgG antibodies. hnRNP K-associated mRNAs were then compared between control and BDNF treated neurons. The results were obtained from the quantification of four different experiments performed in independent preparations, and are expressed as -log ( p value) and log fold change (BDNF vs control). A total of 9509 transcripts showed a decrease in the interaction with hnRNP K in cells stimulated with BDNF; p

    Article Snippet: The Whole Rat Genome Microarray kit (4 × 44K, Agilent Technologies) was used and analyzed with a high-resolution microarray scanner (G2565AA, Agilent Technologies). hnRNP K-bound mRNAs were identified by setting a cutoff value of the fold variation between the hnRNP K and IgG samples.

    Techniques: Cell Culture, Immunoprecipitation, Microarray

    Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and microarray experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P

    Journal: Neuroscience Bulletin

    Article Title: Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

    doi: 10.1007/s12264-018-0251-5

    Figure Lengend Snippet: Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and microarray experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P

    Article Snippet: Chips were washed and scanned by a microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Microarray

    Differentially-expressed genes in the hippocampus of Np65 -KO mice. A Scatter plot of normalized intensity derived from microarray chips with WT and Np65 -KO mice. Dots above the red line denote upregulated genes, and dots below the green line denote downregulated genes. B Venn diagram showing the percentages of differentially-expressed genes categorized by fold-change. C Hierarchical clustering of differentially-expressed genes. N1–N3, Np65 -KO mice; W1–W3, WT mice. D Fold changes of differentially-expressed genes in Np65 -KO mice. E Chromosome distributions of differentially-expressed genes in Np65 -KO mice. Red bars, numbers of upregulated genes; green bars, numbers of downregulated genes.

    Journal: Neuroscience Bulletin

    Article Title: Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

    doi: 10.1007/s12264-018-0251-5

    Figure Lengend Snippet: Differentially-expressed genes in the hippocampus of Np65 -KO mice. A Scatter plot of normalized intensity derived from microarray chips with WT and Np65 -KO mice. Dots above the red line denote upregulated genes, and dots below the green line denote downregulated genes. B Venn diagram showing the percentages of differentially-expressed genes categorized by fold-change. C Hierarchical clustering of differentially-expressed genes. N1–N3, Np65 -KO mice; W1–W3, WT mice. D Fold changes of differentially-expressed genes in Np65 -KO mice. E Chromosome distributions of differentially-expressed genes in Np65 -KO mice. Red bars, numbers of upregulated genes; green bars, numbers of downregulated genes.

    Article Snippet: Chips were washed and scanned by a microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA).

    Techniques: Mouse Assay, Derivative Assay, Microarray

    Differentially-expressed genes in the hippocampus of Np65 -KO mice. A Scatter plot of normalized intensity derived from microarray chips with WT and Np65 -KO mice. Dots above the red line denote upregulated genes, and dots below the green line denote downregulated genes. B Venn diagram showing the percentages of differentially-expressed genes categorized by fold-change. C Hierarchical clustering of differentially-expressed genes. N1–N3, Np65 -KO mice; W1–W3, WT mice. D Fold changes of differentially-expressed genes in Np65 -KO mice. E Chromosome distributions of differentially-expressed genes in Np65 -KO mice. Red bars, numbers of upregulated genes; green bars, numbers of downregulated genes.

    Journal: Neuroscience Bulletin

    Article Title: Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

    doi: 10.1007/s12264-018-0251-5

    Figure Lengend Snippet: Differentially-expressed genes in the hippocampus of Np65 -KO mice. A Scatter plot of normalized intensity derived from microarray chips with WT and Np65 -KO mice. Dots above the red line denote upregulated genes, and dots below the green line denote downregulated genes. B Venn diagram showing the percentages of differentially-expressed genes categorized by fold-change. C Hierarchical clustering of differentially-expressed genes. N1–N3, Np65 -KO mice; W1–W3, WT mice. D Fold changes of differentially-expressed genes in Np65 -KO mice. E Chromosome distributions of differentially-expressed genes in Np65 -KO mice. Red bars, numbers of upregulated genes; green bars, numbers of downregulated genes.

    Article Snippet: Chips were washed and scanned by a microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA).

    Techniques: Mouse Assay, Derivative Assay, Microarray

    Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and microarray experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P

    Journal: Neuroscience Bulletin

    Article Title: Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

    doi: 10.1007/s12264-018-0251-5

    Figure Lengend Snippet: Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and microarray experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P

    Article Snippet: Chips were washed and scanned by a microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Microarray

    Candidate genes identified by microarray approaches. a False colour representation of the 13 overlapping genes (see also intersection in the Venn diagram) identified with sex-biased expression in 2 as well as in 8 months old mice. Fold-changes were calculated from four technical replicates using pooled total RNA isolated from male and female whole mouse hearts of both age groups ( n = 6 per group). Numbers of genes identified with sex-biased expression in each group (90 and 33, respectively) are shown in the Venn diagram. b False colour representation of the 14 overlapping genes (see also intersection in the Venn diagram) identified with sex-biased expression in human left ventricular samples of both age groups. Fold-changes were calculated from four technical replicates using pooled total RNA isolated from three to five samples per group. Numbers of genes identified with sex-biased expression in each age group (93 and 125, respectively) are shown in the Venn diagram. c False colour representation of the 16 genes identified in the Cardiogenomics dataset with sex-biased expression in myocardial tissue of male (mean age = 53.6 years) and female (mean age = 49.3 years) human donors ( n = 7 per group). Note that for some genes sex-biased expression was detected in both species (e.g. Ddx3y and Jarid1d) as well as in both platforms (USP9Y and RPS4Y1). Shades of red represent female-biased expression and blue , male-biased expression in individual samples (FDR

    Journal: Journal of Molecular Medicine (Berlin, Germany)

    Article Title: Sexually dimorphic gene expression in the heart of mice and men

    doi: 10.1007/s00109-007-0240-z

    Figure Lengend Snippet: Candidate genes identified by microarray approaches. a False colour representation of the 13 overlapping genes (see also intersection in the Venn diagram) identified with sex-biased expression in 2 as well as in 8 months old mice. Fold-changes were calculated from four technical replicates using pooled total RNA isolated from male and female whole mouse hearts of both age groups ( n = 6 per group). Numbers of genes identified with sex-biased expression in each group (90 and 33, respectively) are shown in the Venn diagram. b False colour representation of the 14 overlapping genes (see also intersection in the Venn diagram) identified with sex-biased expression in human left ventricular samples of both age groups. Fold-changes were calculated from four technical replicates using pooled total RNA isolated from three to five samples per group. Numbers of genes identified with sex-biased expression in each age group (93 and 125, respectively) are shown in the Venn diagram. c False colour representation of the 16 genes identified in the Cardiogenomics dataset with sex-biased expression in myocardial tissue of male (mean age = 53.6 years) and female (mean age = 49.3 years) human donors ( n = 7 per group). Note that for some genes sex-biased expression was detected in both species (e.g. Ddx3y and Jarid1d) as well as in both platforms (USP9Y and RPS4Y1). Shades of red represent female-biased expression and blue , male-biased expression in individual samples (FDR

    Article Snippet: After washing, microarray slides were scanned using a microarray scanner (G2565BA, Agilent) and image analysis was performed with the Feature Extraction software 7.1.1 (Agilent).

    Techniques: Microarray, Expressing, Mouse Assay, Isolation

    Experimental design to screen for genes with sex-biased expression in the heart of mice and human donors. Total RNA was isolated from mouse whole heart samples and from myocardial tissue of human donors. Cyanine dye labelled cDNAs generated from pooled RNAs were hybridised on Agilent cDNA microarrays. In addition, human myocardial expression datasets were downloaded from CardioGenomics and filtered for sex-biased genes. Candidate gene lists were compared to identify sexual dimorphisms in young as well as aged individuals and both species and platforms. Expression levels of several candidate genes were quantified individually by real-time PCR (QPCR) in the same samples used for microarray analysis and in left ventricular samples from female mice at different stages of the oestrous as well as from males ( n = 8 per group). Numbers of individuals analysed by QPCR are shown in brackets . FDR , false discovery rate; y , years; mon , months

    Journal: Journal of Molecular Medicine (Berlin, Germany)

    Article Title: Sexually dimorphic gene expression in the heart of mice and men

    doi: 10.1007/s00109-007-0240-z

    Figure Lengend Snippet: Experimental design to screen for genes with sex-biased expression in the heart of mice and human donors. Total RNA was isolated from mouse whole heart samples and from myocardial tissue of human donors. Cyanine dye labelled cDNAs generated from pooled RNAs were hybridised on Agilent cDNA microarrays. In addition, human myocardial expression datasets were downloaded from CardioGenomics and filtered for sex-biased genes. Candidate gene lists were compared to identify sexual dimorphisms in young as well as aged individuals and both species and platforms. Expression levels of several candidate genes were quantified individually by real-time PCR (QPCR) in the same samples used for microarray analysis and in left ventricular samples from female mice at different stages of the oestrous as well as from males ( n = 8 per group). Numbers of individuals analysed by QPCR are shown in brackets . FDR , false discovery rate; y , years; mon , months

    Article Snippet: After washing, microarray slides were scanned using a microarray scanner (G2565BA, Agilent) and image analysis was performed with the Feature Extraction software 7.1.1 (Agilent).

    Techniques: Expressing, Mouse Assay, Isolation, Generated, Real-time Polymerase Chain Reaction, Microarray