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Agilent technologies g2565aa microarray scanner
G2565aa Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/g2565aa microarray scanner/product/Agilent technologies
Average 97 stars, based on 42 article reviews
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g2565aa microarray scanner - by Bioz Stars, 2020-04
97/100 stars

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Amplification:

Article Title: Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions
Article Snippet: Half a µg of RNA was amplified and labeled with the Agilent Low Input Quick Amp Labeling Kit. .. Then, slides were scanned at 5 µm resolution in an Agilent G2565AA microarray scanner, and image files were analyzed with the Feature Extraction software 9.5.1.

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Fluorescence-labeled cRNA target was prepared from 500 ng total RNA using Agilent Low Linear amplification kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturers’ instructions. .. Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies).

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: The purified dye-coupled amplified RNA samples were hybridized to an Agilent 44 K mouse 60-mer oligo microarrays (Agilent Technologies) and were incubated for 17 hours at 60°C. .. The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner.

In Situ:

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: Each oligonucleotide was synthesized in situ on 2.5- × 7.5-cm glass slides by Agilent Technologies (Santa Clara, CA). .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software.

Fluorescence:

Article Title: Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease
Article Snippet: .. An Agilent G2565AA microarray scanner (Agilent Technologies, Palo Alto, USA) was used to read the fluorescence intensities of the hybridized arrays at 635 (Cy5) and 532 (Cy3) nm. .. Spot intensities were visualized by using GenePix Pro 4.1 software (Axon Instruments Inc., Foster City, USA) and spots affected by veils, grains of dust or other contaminants were removed from further analysis.

Article Title: Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation
Article Snippet: .. Fluorescence was recorded using an Agilent G2565AA microarray scanner (Agilent Technologies). .. Image and data analysis Tagged Image File Format images were analyzed using the GenePix Pro 4.0 software (Axon Instruments, Foster City, CA), and the quantified data matrix was loaded into a BioArraySoftware Environment (BASE) [ ].

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Fluorescence-labeled cRNA target was prepared from 500 ng total RNA using Agilent Low Linear amplification kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturers’ instructions. .. Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies).

Article Title: The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Article Snippet: Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner. .. The fluorescence intensities were quantified with QuantArray v3.0 software (PerkinElmer).

Synthesized:

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: Each oligonucleotide was synthesized in situ on 2.5- × 7.5-cm glass slides by Agilent Technologies (Santa Clara, CA). .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software.

Software:

Article Title: Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease
Article Snippet: An Agilent G2565AA microarray scanner (Agilent Technologies, Palo Alto, USA) was used to read the fluorescence intensities of the hybridized arrays at 635 (Cy5) and 532 (Cy3) nm. .. Spot intensities were visualized by using GenePix Pro 4.1 software (Axon Instruments Inc., Foster City, USA) and spots affected by veils, grains of dust or other contaminants were removed from further analysis.

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software. .. Initial data handling and visualization were done with the Matlab software (MathWorks, Natick, MA).

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner. .. Microarray images were analyzed using QuantArray v3.0 software (PerkinElmer).

Article Title: Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions
Article Snippet: .. Then, slides were scanned at 5 µm resolution in an Agilent G2565AA microarray scanner, and image files were analyzed with the Feature Extraction software 9.5.1. .. We used the GeneSpring 12.1 software (Agilent) to perform the interarray analyses.

Article Title: A comprehensive analysis of coregulator recruitment, androgen receptor function and gene expression in prostate cancer
Article Snippet: .. AR target gene Agilent oligoarray data analysis Array images were scanned using the G2565AA Microarray scanner (Agilent Technologies) with the gene expression signal extracted using Agilent’s Feature Extraction 5.1.1 software. .. The expression data were then normalized by quantile normalization following by log2 transformation.

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies). .. Fluorescence intensities were extracted using Gene Pix Pro 4.0 software (Axon Instruments Inc., Foster City, CA).

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner. .. The data were processed and analyzed by Genespring GX software package (Agilent Technologies) and were excluded if their t -test p value was greater than 0.05.

Article Title: The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Article Snippet: Microarray and qRT-PCR analyses DNA microarray analysis was carried out using in-house spotted DNA-microarrays of Saccharomyces cerevisiae , and standard protocols of public domain software used by the NFDM. .. Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner.

Construct:

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: Hybridization in miRNA Microarray and Data Treatment The same RNA pools that were used to construct the sequencing libraries were also subject to microarray analysis. .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner.

Spectroscopy:

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: Dye incorporation was monitored by UV-visible spectroscopy. .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner.

Random Hexamer Labeling:

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: Each microarray experiment represents four biological replicates. cDNA was generated from 30 μg of total RNA by using random hexamer primers and SuperScript II (Invitrogen, Carlsbad, CA). .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software.

Quantitative RT-PCR:

Article Title: The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Article Snippet: Paragraph title: Microarray and qRT-PCR analyses ... Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner.

Purification:

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: The labeled RNAs were purified to remove non-reacted Cy3-ULS to produce a fluorescently-labeled RNA sample for microarray analysis. .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner.

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: The purified dye-coupled amplified RNA samples were hybridized to an Agilent 44 K mouse 60-mer oligo microarrays (Agilent Technologies) and were incubated for 17 hours at 60°C. .. The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner.

Article Title: The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Article Snippet: For transcript profiling, total RNA from early log-phase cultures, either untreated or exposed to 2 mM of FeSO4 , was purified using the RNAeasy kit (QIAGEN), followed by the RNA clean up procedure. .. Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner.

Produced:

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Microarrays were produced at the Swegene DNA Microarray Resource Center, Department of Oncology, Lund University, Sweden ( ). .. Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies).

Microarray:

Article Title: Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease
Article Snippet: .. An Agilent G2565AA microarray scanner (Agilent Technologies, Palo Alto, USA) was used to read the fluorescence intensities of the hybridized arrays at 635 (Cy5) and 532 (Cy3) nm. .. Spot intensities were visualized by using GenePix Pro 4.1 software (Axon Instruments Inc., Foster City, USA) and spots affected by veils, grains of dust or other contaminants were removed from further analysis.

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software. .. Initial data handling and visualization were done with the Matlab software (MathWorks, Natick, MA).

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner. .. Microarray images were analyzed using QuantArray v3.0 software (PerkinElmer).

Article Title: Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions
Article Snippet: .. Then, slides were scanned at 5 µm resolution in an Agilent G2565AA microarray scanner, and image files were analyzed with the Feature Extraction software 9.5.1. .. We used the GeneSpring 12.1 software (Agilent) to perform the interarray analyses.

Article Title: Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation
Article Snippet: .. Fluorescence was recorded using an Agilent G2565AA microarray scanner (Agilent Technologies). .. Image and data analysis Tagged Image File Format images were analyzed using the GenePix Pro 4.0 software (Axon Instruments, Foster City, CA), and the quantified data matrix was loaded into a BioArraySoftware Environment (BASE) [ ].

Article Title: Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
Article Snippet: .. After hybridization in an oven rotating at 15 rpm (Model1012, Sheldon Manufacturing, Cornelius, OR), the slides were washed and scanned with the Agilent G2565AA Microarray Scanner. .. Statistical Analysis As the mRNA levels did not fit a Gaussian distribution, (a) the mRNA levels in each subgroup of samples were expressed as the median and range rather than the mean and coefficient of variation, and (b) relationships between the molecular markers and clinical and histological parameters were analyzed with the chi-square test (link between two qualitative parameters) or the non parametric Mann-Whitney U test (link between one qualitative parameter and one quantitative parameter) [ ].

Article Title: A comprehensive analysis of coregulator recruitment, androgen receptor function and gene expression in prostate cancer
Article Snippet: .. AR target gene Agilent oligoarray data analysis Array images were scanned using the G2565AA Microarray scanner (Agilent Technologies) with the gene expression signal extracted using Agilent’s Feature Extraction 5.1.1 software. .. The expression data were then normalized by quantile normalization following by log2 transformation.

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: .. Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies). .. Fluorescence intensities were extracted using Gene Pix Pro 4.0 software (Axon Instruments Inc., Foster City, CA).

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: .. The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner. .. The data were processed and analyzed by Genespring GX software package (Agilent Technologies) and were excluded if their t -test p value was greater than 0.05.

Article Title: The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Article Snippet: .. Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner. .. The fluorescence intensities were quantified with QuantArray v3.0 software (PerkinElmer).

Article Title: Peroxisome Proliferator-Activated Receptor ? Regulates a MicroRNA-Mediated Signaling Cascade Responsible for Hepatocellular Proliferation ▿Peroxisome Proliferator-Activated Receptor ? Regulates a MicroRNA-Mediated Signaling Cascade Responsible for Hepatocellular Proliferation ▿ †
Article Snippet: .. The slides were washed, dried, and scanned using an Agilent G2565AA microarray scanner (Agilent Technologies). ..

Incubation:

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: Briefly, two µg of total RNA were incubated with Cy3-ULS (1 µl of Cy-ULS for 1 µg of cDNA) for 15 min at 85°C. .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner.

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: The purified dye-coupled amplified RNA samples were hybridized to an Agilent 44 K mouse 60-mer oligo microarrays (Agilent Technologies) and were incubated for 17 hours at 60°C. .. The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner.

Labeling:

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: Aminoallyl-modified dUTP was incorporated into the cDNA at a ratio of 4:1 aa-dUTP:dTTP, and cDNA was labeled with Cy3 or Cy5 monoreactive dyes (Amersham, Piscataway, NJ). .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software.

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: The labeled RNAs were purified to remove non-reacted Cy3-ULS to produce a fluorescently-labeled RNA sample for microarray analysis. .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner.

Article Title: Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions
Article Snippet: To assess the labeling and hybridization efficiencies we used an Agilent Spike-In Kit. .. Then, slides were scanned at 5 µm resolution in an Agilent G2565AA microarray scanner, and image files were analyzed with the Feature Extraction software 9.5.1.

Article Title: Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation
Article Snippet: Samples were labeled with Cy3-dCTP (Amersham) and reference was labeled with Cy5-dCTP. .. Fluorescence was recorded using an Agilent G2565AA microarray scanner (Agilent Technologies).

Article Title: Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
Article Snippet: The labeled solutions were then filtered on a Microcon YLM-30 column (Millipore, Billerica, MA), denatured and hybridized with unlabeled Cot-1 DNA (Invitrogen, Carlsbad, CA) to the CGH arrays. .. After hybridization in an oven rotating at 15 rpm (Model1012, Sheldon Manufacturing, Cornelius, OR), the slides were washed and scanned with the Agilent G2565AA Microarray Scanner.

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Pretreatment of arrays, hybridization, and post-hybridization washes was carried out according to manufacturers’ instructions (Corning ChipShot™ labeling system). .. Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies).

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: Amplified Universal Reference Total RNA (2 μg) as control and the amplified nine RNA samples (2 μg each) were labeled with Cy3 and Cy5, respectively using CyDye™ Post-Labeling Reactive Dye Pack (GE Healthcare) according to the manufacturer's instruction. .. The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner.

Article Title: The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Article Snippet: Forty micrograms of RNA were used to generate labeled cDNA, which were hybridized on the DNA arrays. .. Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner.

Expressing:

Article Title: Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions
Article Snippet: Hybridization and slide washing were performed with the Gene Expression Hybridization Kit (Agilent) and Gene Expression Wash Buffers (Agilent), respectively. .. Then, slides were scanned at 5 µm resolution in an Agilent G2565AA microarray scanner, and image files were analyzed with the Feature Extraction software 9.5.1.

Article Title: A comprehensive analysis of coregulator recruitment, androgen receptor function and gene expression in prostate cancer
Article Snippet: .. AR target gene Agilent oligoarray data analysis Array images were scanned using the G2565AA Microarray scanner (Agilent Technologies) with the gene expression signal extracted using Agilent’s Feature Extraction 5.1.1 software. .. The expression data were then normalized by quantile normalization following by log2 transformation.

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Paragraph title: MD-2−/− expression profile ... Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies).

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner. .. The t -test p value gives a confidence measure on how reproducible an expression level measurement is.

Article Title: Peroxisome Proliferator-Activated Receptor ? Regulates a MicroRNA-Mediated Signaling Cascade Responsible for Hepatocellular Proliferation ▿Peroxisome Proliferator-Activated Receptor ? Regulates a MicroRNA-Mediated Signaling Cascade Responsible for Hepatocellular Proliferation ▿ †
Article Snippet: Paragraph title: Gene expression profiling. ... The slides were washed, dried, and scanned using an Agilent G2565AA microarray scanner (Agilent Technologies).

Sequencing:

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: Hybridization in miRNA Microarray and Data Treatment The same RNA pools that were used to construct the sequencing libraries were also subject to microarray analysis. .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner.

Article Title: Peroxisome Proliferator-Activated Receptor ? Regulates a MicroRNA-Mediated Signaling Cascade Responsible for Hepatocellular Proliferation ▿Peroxisome Proliferator-Activated Receptor ? Regulates a MicroRNA-Mediated Signaling Cascade Responsible for Hepatocellular Proliferation ▿ †
Article Snippet: An Agilent 22 K mouse 60-mer oligonucleotide microarray (Agilent Technologies, Santa Clara, CA) containing more than 20,800 unique well-characterized gene/expressed sequence tag features were used. .. The slides were washed, dried, and scanned using an Agilent G2565AA microarray scanner (Agilent Technologies).

Transformation Assay:

Article Title: A comprehensive analysis of coregulator recruitment, androgen receptor function and gene expression in prostate cancer
Article Snippet: AR target gene Agilent oligoarray data analysis Array images were scanned using the G2565AA Microarray scanner (Agilent Technologies) with the gene expression signal extracted using Agilent’s Feature Extraction 5.1.1 software. .. The expression data were then normalized by quantile normalization following by log2 transformation.

Article Title: Expression of Stanniocalcin 1 in Thyroid Side Population Cells and Thyroid Cancer Cells
Article Snippet: The slides were washed, dried, and scanned using Agilent G2565AA microarray scanner. .. For the retained confidence signals, they were transformed into the log2 ratio relative to the pooled intact thyroid reference sample.

Hybridization:

Article Title: Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease
Article Snippet: Microarray Hybridization kit according to the manufacturer's instructions (Corning, Acton, USA). .. An Agilent G2565AA microarray scanner (Agilent Technologies, Palo Alto, USA) was used to read the fluorescence intensities of the hybridized arrays at 635 (Cy5) and 532 (Cy3) nm.

Article Title: Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides
Article Snippet: .. Hybridization was carried out in the miRNAChip_MS_V1 at 42°C for 16 h. Slides were washed following the manufacture’s recommendations and immediately scanned using an Agilent G2565AA microarray scanner. .. Microarray images were analyzed using QuantArray v3.0 software (PerkinElmer).

Article Title: Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions
Article Snippet: Hybridization and slide washing were performed with the Gene Expression Hybridization Kit (Agilent) and Gene Expression Wash Buffers (Agilent), respectively. .. Then, slides were scanned at 5 µm resolution in an Agilent G2565AA microarray scanner, and image files were analyzed with the Feature Extraction software 9.5.1.

Article Title: Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation
Article Snippet: Hybridization was performed overnight at 42 °C using a MAUI hybridization system (BioMicro Systems, Salt Lake City, USA), and slides were washed according to the Corning Pronto Plus system instructions. .. Fluorescence was recorded using an Agilent G2565AA microarray scanner (Agilent Technologies).

Article Title: Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
Article Snippet: .. After hybridization in an oven rotating at 15 rpm (Model1012, Sheldon Manufacturing, Cornelius, OR), the slides were washed and scanned with the Agilent G2565AA Microarray Scanner. .. Statistical Analysis As the mRNA levels did not fit a Gaussian distribution, (a) the mRNA levels in each subgroup of samples were expressed as the median and range rather than the mean and coefficient of variation, and (b) relationships between the molecular markers and clinical and histological parameters were analyzed with the chi-square test (link between two qualitative parameters) or the non parametric Mann-Whitney U test (link between one qualitative parameter and one quantitative parameter) [ ].

Article Title: Genome wide expression profiling and mutagenesis studies reveal that LPS responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions
Article Snippet: Pretreatment of arrays, hybridization, and post-hybridization washes was carried out according to manufacturers’ instructions (Corning ChipShot™ labeling system). .. Hybridized microarrays were imaged using an Agilent G2565AA microarray scanner (Agilent Technologies).

Generated:

Article Title: Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease
Article Snippet: An Agilent G2565AA microarray scanner (Agilent Technologies, Palo Alto, USA) was used to read the fluorescence intensities of the hybridized arrays at 635 (Cy5) and 532 (Cy3) nm. .. The data generated via GenePix Pro were analysed by using the Bio Array Software Environment BASE ( ).

Article Title: The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
Article Snippet: Each microarray experiment represents four biological replicates. cDNA was generated from 30 μg of total RNA by using random hexamer primers and SuperScript II (Invitrogen, Carlsbad, CA). .. Data acquisition was performed by using an Agilent G2565AA microarray scanner and extracted by using Agilent feature extraction software.

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    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g2565ca dna microarray scanner/product/Agilent technologies
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    g2565ca dna microarray scanner - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Principle of multiplex SOMAmer affinity assay. (A) Binding. SOMAmers and samples are mixed in 96-well microwell plates and allowed to bind. Cognate and non-cognate SOMAmer-target protein complexes form. Free SOMAmer and protein are also present. (B–H) Schematic sequence of assay steps leading to quantitative readout of target proteins. (B) SOMAmer-protein binding: DNA-based SOMAmer molecules (gold, blue, and green) have unique shapes selected to bind to a specific protein. SOMAmers contain biotin (B), a photo-cleavable linker (L) and a fluorescent tag at the 5′ end. Most SOMAmers (gold and green) bind to cognate proteins (red), but some (blue) form non-cognate complexes. (C) Catch-1. SOMAmers are captured onto a bead coated with streptavidin (SA) which binds biotin. Un-complexed proteins are washed away. (D) Proteins are tagged with NHS-biotin. (E) Photocleavage and kinetic challenge. UV light (hν) cleaves the linker and SOMAmers are released from beads, leaving biotin on bead. Samples are challenged with anionic competitor (dextran sulfate). Non-cognate complexes (blue SOMAmer) preferentially dissociate. (F) Catch-2 SOMAmer-protein complexes are captured onto new avidin coated beads by protein biotin tag. Free SOMAmers are washed away. (G) SOMAmers are released from complexes into solution at high pH. (H) Remaining SOMAmers are quantified by hybridization to microarray containing single-stranded DNA probes complementary to SOMAmer DNA sequence, which form a double-stranded helix. Hybridized SOMAmers are detected by fluorescent tags when the array is scanned.

    Journal: PLoS ONE

    Article Title: Aptamer-Based Multiplexed Proteomic Technology for Biomarker DiscoveryUnlocking Biomarker Discovery: Large Scale Application of Aptamer Proteomic Technology for Early Detection of Lung Cancer

    doi: 10.1371/journal.pone.0015004

    Figure Lengend Snippet: Principle of multiplex SOMAmer affinity assay. (A) Binding. SOMAmers and samples are mixed in 96-well microwell plates and allowed to bind. Cognate and non-cognate SOMAmer-target protein complexes form. Free SOMAmer and protein are also present. (B–H) Schematic sequence of assay steps leading to quantitative readout of target proteins. (B) SOMAmer-protein binding: DNA-based SOMAmer molecules (gold, blue, and green) have unique shapes selected to bind to a specific protein. SOMAmers contain biotin (B), a photo-cleavable linker (L) and a fluorescent tag at the 5′ end. Most SOMAmers (gold and green) bind to cognate proteins (red), but some (blue) form non-cognate complexes. (C) Catch-1. SOMAmers are captured onto a bead coated with streptavidin (SA) which binds biotin. Un-complexed proteins are washed away. (D) Proteins are tagged with NHS-biotin. (E) Photocleavage and kinetic challenge. UV light (hν) cleaves the linker and SOMAmers are released from beads, leaving biotin on bead. Samples are challenged with anionic competitor (dextran sulfate). Non-cognate complexes (blue SOMAmer) preferentially dissociate. (F) Catch-2 SOMAmer-protein complexes are captured onto new avidin coated beads by protein biotin tag. Free SOMAmers are washed away. (G) SOMAmers are released from complexes into solution at high pH. (H) Remaining SOMAmers are quantified by hybridization to microarray containing single-stranded DNA probes complementary to SOMAmer DNA sequence, which form a double-stranded helix. Hybridized SOMAmers are detected by fluorescent tags when the array is scanned.

    Article Snippet: Microarray Imaging The microarray slides were imaged with a microarray scanner (Agilent G2565CA Microarray Scanner System, Agilent Technologies) in the Cy3-channel at 5 µm resolution at 100% PMT setting and the XRD option enabled at 0.05.

    Techniques: Multiplex Assay, Binding Assay, Sequencing, Protein Binding, Avidin-Biotin Assay, Hybridization, Microarray

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization