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Agilent technologies g2565aa g2565ab scanner
G2565aa G2565ab Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g2565aa g2565ab scanner - by Bioz Stars, 2020-03
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Centrifugation:

Article Title: Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
Article Snippet: Microarrays were incubated in a hybridization chamber (Genetix) with 20 μl of hybridization buffer (6 × SSC, 0.5% SDS, 1% BSA) under a 24 × 24 mm cover slip, and bathed at 42°C for 45 min. Then the microarrays were washed with distilled water, and dried by a brief centrifugation. .. The slides were dried by spinning 1 min. at 500 × g and, finally, scanned using a G2565AA/G2565AB Scanner (Agilent).

Size-exclusion Chromatography:

Article Title: Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
Article Snippet: After a 3 hours incubation in the hybridization chamber, the slides were washed for 5 min. in 2 × SSC, 0.1% lauroylsarcosine, followed by 5 min. in 2 × SSC, and finally rinsed 10 sec. in 0.2 × SSC, and 5 min. in distilled water, at 45°C. .. The slides were dried by spinning 1 min. at 500 × g and, finally, scanned using a G2565AA/G2565AB Scanner (Agilent).

Labeling:

Article Title: Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
Article Snippet: The hybridization with the labeled DNA was carried out in hybridization buffer at the appropriate temperature (58–60°C) and with the required amount of target (0.3 pmoles Alexa Fluor 647 equivalent to 50 ng). .. The slides were dried by spinning 1 min. at 500 × g and, finally, scanned using a G2565AA/G2565AB Scanner (Agilent).

Incubation:

Article Title: Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
Article Snippet: After a 3 hours incubation in the hybridization chamber, the slides were washed for 5 min. in 2 × SSC, 0.1% lauroylsarcosine, followed by 5 min. in 2 × SSC, and finally rinsed 10 sec. in 0.2 × SSC, and 5 min. in distilled water, at 45°C. .. The slides were dried by spinning 1 min. at 500 × g and, finally, scanned using a G2565AA/G2565AB Scanner (Agilent).

Hybridization:

Article Title: Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
Article Snippet: Paragraph title: Hybridization and scanning ... The slides were dried by spinning 1 min. at 500 × g and, finally, scanned using a G2565AA/G2565AB Scanner (Agilent).

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  • 99
    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-03
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    99
    Agilent technologies dna microarray scanner g2565ba
    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through <t>DNA</t> <t>microarray</t> analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
    Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner g2565ba/product/Agilent technologies
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner g2565ba - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    87
    Agilent technologies microarray scanner g model g2565ba
    CD6 co-stimulation with activated leucocyte-cell adhesion molecule (ALCAM) induces a distinct gene transcription profile which is amplified further by interleukin (IL)-2. Difference in gene expression profile between 3 × 10 4 freshly isolated human peripheral blood mononuclear cells (PBMC) stimulated for 72 h with the immobilized anti-CD3 monoclonal antibody (mAb) (0·25 µg/ml) alone (lane 1), with ALCAM-Fc (1 µg/ml) (lane 2), interleukin (IL)-2 (2·5 ng/ml) (lane 4) or both (lane 3) or compared to unstimulated cells (lane 5). (a) Clustering of 15 000 genes or (b) 60 selected genes regulated differentially relative to the unstimulated cells are depicted. (c) Selected genes expression in CD3-activated cells incubated with a soluble CD6 membrane-distal domain specific humanized immunoglobulin (Ig)G1 non-depleting mAb (lane 7) or an irrelevant isotype control mAb (lane 6) at 10 µg/ml, compared to unstimulated cells (lane 8). (d) Colour bar ranging from 0 to 15-fold change relative to control. RNA was extracted by using Trizol reagent protocol. The labelled RNA was hybridized onto the human gene expression array 8 × 15 K. Hybridization was carried out in Agilent's Surehyb Chambers at 65°C for 16 h. The hybridized slides were washed and scanned using Agilent <t>microarray</t> <t>scanner</t> G Model <t>G2565BA</t> at 5 micron resolution.
    Microarray Scanner G Model G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner g model g2565ba/product/Agilent technologies
    Average 87 stars, based on 5 article reviews
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    99
    Agilent technologies microarray scanner
    Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and <t>microarray</t> experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-03
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      Buy from Supplier

    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: RNA Expression, Microarray, Expressing

    Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: Expressing, Microarray

    CD6 co-stimulation with activated leucocyte-cell adhesion molecule (ALCAM) induces a distinct gene transcription profile which is amplified further by interleukin (IL)-2. Difference in gene expression profile between 3 × 10 4 freshly isolated human peripheral blood mononuclear cells (PBMC) stimulated for 72 h with the immobilized anti-CD3 monoclonal antibody (mAb) (0·25 µg/ml) alone (lane 1), with ALCAM-Fc (1 µg/ml) (lane 2), interleukin (IL)-2 (2·5 ng/ml) (lane 4) or both (lane 3) or compared to unstimulated cells (lane 5). (a) Clustering of 15 000 genes or (b) 60 selected genes regulated differentially relative to the unstimulated cells are depicted. (c) Selected genes expression in CD3-activated cells incubated with a soluble CD6 membrane-distal domain specific humanized immunoglobulin (Ig)G1 non-depleting mAb (lane 7) or an irrelevant isotype control mAb (lane 6) at 10 µg/ml, compared to unstimulated cells (lane 8). (d) Colour bar ranging from 0 to 15-fold change relative to control. RNA was extracted by using Trizol reagent protocol. The labelled RNA was hybridized onto the human gene expression array 8 × 15 K. Hybridization was carried out in Agilent's Surehyb Chambers at 65°C for 16 h. The hybridized slides were washed and scanned using Agilent microarray scanner G Model G2565BA at 5 micron resolution.

    Journal: Clinical and Experimental Immunology

    Article Title: CD6 synergistic co-stimulation promoting proinflammatory response is modulated without interfering with the activated leucocyte cell adhesion molecule interaction

    doi: 10.1111/j.1365-2249.2010.04235.x

    Figure Lengend Snippet: CD6 co-stimulation with activated leucocyte-cell adhesion molecule (ALCAM) induces a distinct gene transcription profile which is amplified further by interleukin (IL)-2. Difference in gene expression profile between 3 × 10 4 freshly isolated human peripheral blood mononuclear cells (PBMC) stimulated for 72 h with the immobilized anti-CD3 monoclonal antibody (mAb) (0·25 µg/ml) alone (lane 1), with ALCAM-Fc (1 µg/ml) (lane 2), interleukin (IL)-2 (2·5 ng/ml) (lane 4) or both (lane 3) or compared to unstimulated cells (lane 5). (a) Clustering of 15 000 genes or (b) 60 selected genes regulated differentially relative to the unstimulated cells are depicted. (c) Selected genes expression in CD3-activated cells incubated with a soluble CD6 membrane-distal domain specific humanized immunoglobulin (Ig)G1 non-depleting mAb (lane 7) or an irrelevant isotype control mAb (lane 6) at 10 µg/ml, compared to unstimulated cells (lane 8). (d) Colour bar ranging from 0 to 15-fold change relative to control. RNA was extracted by using Trizol reagent protocol. The labelled RNA was hybridized onto the human gene expression array 8 × 15 K. Hybridization was carried out in Agilent's Surehyb Chambers at 65°C for 16 h. The hybridized slides were washed and scanned using Agilent microarray scanner G Model G2565BA at 5 micron resolution.

    Article Snippet: Hybridization was carried out in Agilent's Surehyb chambers at 65°C for 16 h. The hybridized slides were washed using Agilent gene expression wash buffers (part no. 5188-5327) and scanned using the Agilent microarray scanner G model G2565BA at 5 micron resolution.

    Techniques: Amplification, Expressing, Isolation, Incubation, Hybridization, Microarray

    Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and microarray experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P

    Journal: Neuroscience Bulletin

    Article Title: Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

    doi: 10.1007/s12264-018-0251-5

    Figure Lengend Snippet: Relative expression levels of selected genes in Np65 -KO and WT mice. A Results of quantitative real-time PCR (RT-PCR) ( n = 4 mice). B RT-PCR and microarray experimental results for relative gene expression in Np65 -KO and WT mice. The relative expression levels were calculated as the ratio of the target gene expression level to the β-actin expression level in the same sample. Fold changes are shown as mean ± SEM. * P

    Article Snippet: Chips were washed and scanned by a microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA).

    Techniques: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Microarray

    Differentially-expressed genes in the hippocampus of Np65 -KO mice. A Scatter plot of normalized intensity derived from microarray chips with WT and Np65 -KO mice. Dots above the red line denote upregulated genes, and dots below the green line denote downregulated genes. B Venn diagram showing the percentages of differentially-expressed genes categorized by fold-change. C Hierarchical clustering of differentially-expressed genes. N1–N3, Np65 -KO mice; W1–W3, WT mice. D Fold changes of differentially-expressed genes in Np65 -KO mice. E Chromosome distributions of differentially-expressed genes in Np65 -KO mice. Red bars, numbers of upregulated genes; green bars, numbers of downregulated genes.

    Journal: Neuroscience Bulletin

    Article Title: Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

    doi: 10.1007/s12264-018-0251-5

    Figure Lengend Snippet: Differentially-expressed genes in the hippocampus of Np65 -KO mice. A Scatter plot of normalized intensity derived from microarray chips with WT and Np65 -KO mice. Dots above the red line denote upregulated genes, and dots below the green line denote downregulated genes. B Venn diagram showing the percentages of differentially-expressed genes categorized by fold-change. C Hierarchical clustering of differentially-expressed genes. N1–N3, Np65 -KO mice; W1–W3, WT mice. D Fold changes of differentially-expressed genes in Np65 -KO mice. E Chromosome distributions of differentially-expressed genes in Np65 -KO mice. Red bars, numbers of upregulated genes; green bars, numbers of downregulated genes.

    Article Snippet: Chips were washed and scanned by a microarray scanner (G2565BA, Agilent Technologies, Santa Clara, CA).

    Techniques: Mouse Assay, Derivative Assay, Microarray