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Agilent technologies g2565aa dna microarray scanner
G2565aa Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g2565aa dna microarray scanner - by Bioz Stars, 2020-04
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Hybridization:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Paragraph title: RNA isolation and Hybridization ... Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Article Title: Antibody Arrays Identify Potential Diagnostic Markers of Hepatocellular Carcinoma
Article Snippet: The slides were incubated overnight using gentle rotation at 4 rpm in Hybridization Incubator (Robbins Scientific, Model 400). .. Finally, the arrays were scanned in an Agilent G2565AA DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 100%, 10%, and 2% PMT individually using 5 μm resolution with only the green channel on.

Article Title: Improved microarray gene expression profiling of virus-infected cells after removal of viral RNA
Article Snippet: .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner. .. Affymetrix arrays mRNA amplification was performed using the MessageAmp™ II Biotin Enhanced kit from Ambion, according to the manufacturer's instructions.

Article Title: Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
Article Snippet: .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner. .. Statistical analysis After data extraction using Imagene 5.6 Software (BioDiscovery), Lowess normalization [ ] was performed on mean spot-intensities in order to correct for dye and print-tip biases [ ].

Article Title: Dissection of a metastatic gene expression signature into distinct components
Article Snippet: Microarray hybridization was performed as described elsewhere [ ]. .. The slides were scanned in the Agilent G2565AA DNA Microarray Scanner.

Amplification:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: RNA was amplified in a single round and complementary DNA (cDNA) was synthesized with Superscript III reverse transcriptase (Invitrogen) using a T7 oligo(dT)24VN primer . .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Article Title: Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
Article Snippet: Briefly, mRNA was amplified from 1 μg of total RNA by cDNA synthesis with oligo(dT) double-anchored primers, followed by in vitro transcription using a T7 RNA polymerase kit (Ambion). .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner.

In Vitro:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Complementary DNA was transcribed in vitro using the T7Megascript kit (Ambion) in the presence of aminoallyl-UTP, and copy ribonucleic acid (cRNA) quality was evaluated using the Bioanalyzer. .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Article Title: Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
Article Snippet: Briefly, mRNA was amplified from 1 μg of total RNA by cDNA synthesis with oligo(dT) double-anchored primers, followed by in vitro transcription using a T7 RNA polymerase kit (Ambion). .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner.

Synthesized:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: RNA was amplified in a single round and complementary DNA (cDNA) was synthesized with Superscript III reverse transcriptase (Invitrogen) using a T7 oligo(dT)24VN primer . .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Isolation:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Paragraph title: RNA isolation and Hybridization ... Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Blocking Assay:

Article Title: Antibody Arrays Identify Potential Diagnostic Markers of Hepatocellular Carcinoma
Article Snippet: Next, biotinylated detection antibodies were diluted 1:90 with binding buffer (0.4% triton X-100 and 1% casein block buffer). .. Finally, the arrays were scanned in an Agilent G2565AA DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 100%, 10%, and 2% PMT individually using 5 μm resolution with only the green channel on.

Mouse Assay:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Each cRNA sample was labeled with Cy3 or Cy5 and was hybridized in dye swap against a common reference pool sample consisting of RNA from naïve mice; a total of 6 slides were hybridized for each experimental group. .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Labeling:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Each cRNA sample was labeled with Cy3 or Cy5 and was hybridized in dye swap against a common reference pool sample consisting of RNA from naïve mice; a total of 6 slides were hybridized for each experimental group. .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Article Title: Improved microarray gene expression profiling of virus-infected cells after removal of viral RNA
Article Snippet: Mouse slides containing 35,000 spots (32,101 70-mer oligonucleotides, and 2,891 control spots) were hybridized with 1 μg of each alternatively labeled cRNA target at 42°C for 16–20 h using LifterSlips (Erie Scientific) and Corning Hybridization Chambers [ ]. .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner.

Article Title: Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
Article Snippet: Corning UltraGAPS slides, printed with a Mouse Array-Ready Oligo set (Operon; 35,000 spots), were hybridized with 1 μg of each alternatively labeled cRNA target at 42°C for 16–20 h. Two independent dye-swap hybridizations (4 arrays) were performed for each experimental group. .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner.

Article Title: Dissection of a metastatic gene expression signature into distinct components
Article Snippet: We combined 300 ng of cy5 or cy3 labeled sample target (with a labeled nucleotide incorporation of 3% to 5%) with 300 ng reverse labeled reference cRNA [ ], which was then fragmented using Ambion's Fragmentation kit (Ambion, Austin, TX, USA). .. The slides were scanned in the Agilent G2565AA DNA Microarray Scanner.

Microarray:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip . .. The microarray data were analyzed by ANOVA-modeling to identify genes that show differential expression from naïve control mice.

Article Title: Antibody Arrays Identify Potential Diagnostic Markers of Hepatocellular Carcinoma
Article Snippet: .. Finally, the arrays were scanned in an Agilent G2565AA DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 100%, 10%, and 2% PMT individually using 5 μm resolution with only the green channel on. ..

Article Title: Improved microarray gene expression profiling of virus-infected cells after removal of viral RNA
Article Snippet: .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner. .. Affymetrix arrays mRNA amplification was performed using the MessageAmp™ II Biotin Enhanced kit from Ambion, according to the manufacturer's instructions.

Article Title: Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
Article Snippet: .. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner. .. Statistical analysis After data extraction using Imagene 5.6 Software (BioDiscovery), Lowess normalization [ ] was performed on mean spot-intensities in order to correct for dye and print-tip biases [ ].

Article Title: Dissection of a metastatic gene expression signature into distinct components
Article Snippet: .. The slides were scanned in the Agilent G2565AA DNA Microarray Scanner. .. Images were quantified and corrected for background using Imagene software (Biodiscovery, El Segundo, CA, USA).

Incubation:

Article Title: Antibody Arrays Identify Potential Diagnostic Markers of Hepatocellular Carcinoma
Article Snippet: After incubation, the arrays were rinsed in PBST, washed twice in PBST (10 min each), once in PBS (10 min), and once in distilled water for 1 minute before being spun dry. .. Finally, the arrays were scanned in an Agilent G2565AA DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 100%, 10%, and 2% PMT individually using 5 μm resolution with only the green channel on.

Article Title: Dissection of a metastatic gene expression signature into distinct components
Article Snippet: Before hybridization, the microarray slides were incubated in borohydrate buffer (2× SSC (0.3 M NaCl, 50 mM sodium citrate), 0.05% SDS and 0.25% w/v sodium borohydrate (Sigma-Aldrich, St. Louis, MO, USA) for 30 minutes at 42°C. .. The slides were scanned in the Agilent G2565AA DNA Microarray Scanner.

Spectrophotometry:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Cy3 or Cy5 fluorophores (Amersham Biosciences, NL) were coupled to 1500 ng cRNA, and label incorporation was monitored by spectrophotometry and hybridizations were set up with 1000 ng of Cy3-labeled and 1000 ng of Cy5-labeled cRNA. .. Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip .

Expressing:

Article Title: Amygdala 14-3-3? as a Novel Modulator of Escalating Alcohol Intake in Mice
Article Snippet: Slides were washed manually, scanned in the Agilent G2565AA DNA Microarray Scanner (100% laser power and 30% photomultiplier tube) and quantified and background corrected with IMAGENE (version 5.6.1; BioDiscovery, Inc., CA, USA) and Loess normalized per print-tip . .. The microarray data were analyzed by ANOVA-modeling to identify genes that show differential expression from naïve control mice.

Article Title: Dissection of a metastatic gene expression signature into distinct components
Article Snippet: Paragraph title: Gene expression analysis ... The slides were scanned in the Agilent G2565AA DNA Microarray Scanner.

Binding Assay:

Article Title: Antibody Arrays Identify Potential Diagnostic Markers of Hepatocellular Carcinoma
Article Snippet: Next, biotinylated detection antibodies were diluted 1:90 with binding buffer (0.4% triton X-100 and 1% casein block buffer). .. Finally, the arrays were scanned in an Agilent G2565AA DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 100%, 10%, and 2% PMT individually using 5 μm resolution with only the green channel on.

Ab Array:

Article Title: Antibody Arrays Identify Potential Diagnostic Markers of Hepatocellular Carcinoma
Article Snippet: Paragraph title: Antibody array processing ... Finally, the arrays were scanned in an Agilent G2565AA DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 100%, 10%, and 2% PMT individually using 5 μm resolution with only the green channel on.

Software:

Article Title: Dissection of a metastatic gene expression signature into distinct components
Article Snippet: The slides were scanned in the Agilent G2565AA DNA Microarray Scanner. .. Images were quantified and corrected for background using Imagene software (Biodiscovery, El Segundo, CA, USA).

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  • 99
    Agilent technologies dna microarray scanner
    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from <t>DNA</t> <t>microarray</t> data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the <t>microarray</t> data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    Agilent technologies g2565ca dna microarray scanner
    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of <t>microarray</t> probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic <t>DNA</t> extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.
    G2565ca Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g2565ca dna microarray scanner/product/Agilent technologies
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    g2565ca dna microarray scanner - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    94
    Agilent technologies dna microarray scanner g2565ba
    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through <t>DNA</t> <t>microarray</t> analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.
    Dna Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner g2565ba/product/Agilent technologies
    Average 94 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner g2565ba - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Journal: Inhalation Toxicology

    Article Title: Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity

    doi: 10.3109/08958378.2015.1026620

    Figure Lengend Snippet: Effects of CNT-1 and CNT-2 on gene expression in NR8383 cells. Venn diagram showing the number of upregulated and downregulated genes in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h (A). Heat map generated from DNA microarray data reflecting differential expression of genes involved in the response to stimulus (GO: 0050896) in NR8383 cells exposed to CNT-1 or CNT-2 for 24 h. Expression levels are colored blue for low intensity and red for high intensity (see scale at the bottom right corner) (B).

    Article Snippet: Each labeled cRNA probe was used separately for hybridization to a 4 × 44 K Whole Rat Genome Microarray Kit (G4131F; Agilent Technologies, Santa Clara, CA), and hybridization was performed at 65 °C for 17 h. Hybridized microarray slides were washed according to the instructions of the manufacturer and were scanned with an Agilent DNA Microarray Scanner (G2565BA; Agilent Technologies, Santa Clara, CA) at 5-μm resolution.

    Techniques: Expressing, Generated, Microarray

    Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Journal: Scientific Reports

    Article Title: Transcriptomics in lung tissue upon respiratory syncytial virus infection reveals aging as important modulator of immune activation and matrix maintenance

    doi: 10.1038/s41598-018-35180-2

    Figure Lengend Snippet: Distinct effects by aging and RSV on gene expression. Panel A shows each individual animal in a principal component analysis (PCA) of the microarray data of lung samples derived at day 0 (mock-inoculated, black, n = 4/group), or at day 2 (red, n = 5/group) or day 5 (green, n = 5/group) after inoculation with RSV. Aging-regulated expression appears by PC1, RSV-regulated gene expression appears along PC2. Y, young (circles); O, old (triangles); M, mock control; R, RSV-inoculated. Panel B shows a Venn-diagram depicting numbers of genes that are significantly upregulated (↑), downregulated (↓), or show no significant response (−). The three symbols indicate from left to right: response to RSV in young mice, response to RSV in old mice, response to aging, respectively.

    Article Snippet: After washing (NimbleGen Arrays User’s Guide – Gene Expression Arrays Version 5.0), slides were scanned in an ozone-free room with a microarray scanner (Agilent DNA microarray scanner G2565CA, Agilent Technologies).

    Techniques: Expressing, Microarray, Derivative Assay, Mouse Assay

    Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Journal: Nucleic Acids Research

    Article Title: Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum

    doi: 10.1093/nar/gkn1069

    Figure Lengend Snippet: Chromosome aneuploidy in L. infantum Sb2000.1 and Sb4000.4 antimony-resistant mutants. Log 2 -transformed Sb2000.1/WT expression ratios of the upregulated chromosome 1 ( A ), chromosome 11 ( B ) and chromosome 25 ( C ) and of the downregulated chromosome 9 ( D ), chromosome 12 ( E ) and chromosome 32 ( F ) plotted as a function of the location of microarray probes on each chromosomes. For each plot, the log 2 -transformed gene expression ratios of chromosome 30, which was equally expressed in both samples, are shown as a control (grey line). Quantitative Southern blot hybridizations of digested genomic DNA extracted from L. infantum WT (lane 1), Sb2000.1 (lane 2) and Sb4000.4 (lane 3) were performed to correlate gene expression modulation of entire chromosomes with the chromosome DNA copy number. The hybridization signal of LinJ30_V3.2990 was used for normalization. The hybridization signals were quantified using ImageQuant 5.2 (Molecular Dynamics) and the fold differences in DNA copy number of Sb2000.1 compared to WT are found below each blot.

    Article Snippet: Microarray data acquisition and analysis Detection of the Alexa Fluor 555 and Alexa Fluor 647 signals was performed on a G2565CA DNA microarray scanner (Agilent technologies) at a 5-μm resolution.

    Techniques: Transformation Assay, Expressing, Microarray, Southern Blot, Hybridization

    Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Cancer cell AGR2/CD10 RNA expression levels. Levels were determined through DNA microarray analysis with signal intensity values on the y -axis. Indicated on the x -axis are G3 and G4 primary tumor cell types, L luminal, LNCaP, C4-2, CL1, DU145, PC3 cell lines, LuCaP35, LuCaP 49 xenografts. Except for DU145 and LuCaP 49, the expression pattern is either CD10 or AGR2.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: RNA Expression, Microarray, Expressing

    Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Prostate cancer cell phenotypes based on AGR2 and CD10 expression

    doi: 10.1038/modpathol.2012.238

    Figure Lengend Snippet: Expression levels of AGR2 and CD10 in xenografts. As inferred from DNA microarray analysis, these levels are presented in histogram format (top). CY3 intensity values are indicated on the y -axis. Because of the lower values for CD10, a separate histogram for CD10 is included (bottom). Note the ~6-fold decrease in CD10 from LuCaP 96 to Lu CaP 96CR.

    Article Snippet: Probe labeling and hybridization was performed following the Agilent protocol, and fluorescent array images were collected using the Agilent DNA microarray scanner G2565BA.

    Techniques: Expressing, Microarray