g0 by trypsination  (Lonza)


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    Name:
    Trypsin EDTA
    Description:
    Trypsin EDTA 10X includes 5g L trypsin 1 250 and 2g L Versene EDTA manufactured with irradiated parcine trypsine tested for porcine parvovirus and mycoplasma 100 mL
    Catalog Number:
    BE02-007E
    Price:
    None
    Category:
    Culture Media and Reagents
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    Structured Review

    Lonza g0 by trypsination
    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in <t>G0</t> by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).
    Trypsin EDTA 10X includes 5g L trypsin 1 250 and 2g L Versene EDTA manufactured with irradiated parcine trypsine tested for porcine parvovirus and mycoplasma 100 mL
    https://www.bioz.com/result/g0 by trypsination/product/Lonza
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g0 by trypsination - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "The Human Homolog of Escherichia coli Endonuclease V Is a Nucleolar Protein with Affinity for Branched DNA Structures"

    Article Title: The Human Homolog of Escherichia coli Endonuclease V Is a Nucleolar Protein with Affinity for Branched DNA Structures

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0047466

    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in G0 by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).
    Figure Legend Snippet: Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in G0 by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).

    Techniques Used: Quantitative RT-PCR, Standard Deviation, Staining, Flow Cytometry, Blocking Assay, Isolation, Electrophoresis, Hybridization

    Related Articles

    Centrifugation:

    Article Title: Phosphorylation of human TRM9L integrates multiple stress-signaling pathways for tumor growth suppression
    Article Snippet: .. Unless otherwise stated, cells were detached from tissue culture plates by trypsinization (0.05% trypsin-EDTA without Ca2+ /Mg2+ , Lonza) at 37°C and collected by centrifugation (500 g , 5 min, 4°C). .. After aspirating medium, the cell pellet was resuspended in lysis buffer consisting of PBS, 1% Triton X-100, 2 mM EDTA, 1:100 mammalian cell protease inhibitors (Sigma-Aldrich), and 1:100 phosphatase inhibitor (Halt, Thermo Fisher Scientific).

    Cell Cycle Assay:

    Article Title: Diatom-Derived Polyunsaturated Aldehydes Activate Cell Death in Human Cancer Cell Lines but Not Normal Cells
    Article Snippet: Densitometric analysis of immunopositive bands was performed using Image J software. .. Cell cycle analysis A549 cells were collected from plates using 1 mL of Trypsin-EDTA (Lonza, Italy), fixed in 70% ethanol and stored at −20°C. .. Cells were then washed twice with PBS, resuspended in PBS containing 1 mg ml−1 RNase A (Qiagen, Cat.19101), incubated at 37°C for 45 min and then stained with propidium iodide (PI, 10 µg ml−1 ) for 15 min.

    Modification:

    Article Title: A microcarrier-based spheroid 3D invasion assay to monitor dynamic cell movement in extracellular matrix
    Article Snippet: In conclusion, this microcarrier-based 3D invasion assay is a powerful tool to study cell invasion in vitro. .. Reagents Dulbecco’s modified Eagle’s medium (DMEM, D0819, Sigma); Trypsin-EDTA (BE-17-161E, Lonza); Dulbecco’s Phosphate Buffered Saline (PBS, Ca2+ and Mg2+ free, D8537, Sigma-Aldrich); Fetal bovine serum (FBS, F7524, Sigma); Collagen type I, rat tail (08–115; Millipore); Matrigel Growth factor reduced (356,231, Coring); Agar (A1296, Sigma-Aldrich); Sodium bicarbonate (11810–017, Life technologies). .. Imaging System and Climate Control Configuration As time-lapse imaging may take hours to days, a screening system, e.g. confocal microscope, integrated with a cell incubation setup is indispensable.

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    Lonza g0 by trypsination
    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in <t>G0</t> by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).
    G0 By Trypsination, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g0 by trypsination/product/Lonza
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g0 by trypsination - by Bioz Stars, 2021-05
    97/100 stars
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    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in G0 by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).

    Journal: PLoS ONE

    Article Title: The Human Homolog of Escherichia coli Endonuclease V Is a Nucleolar Protein with Affinity for Branched DNA Structures

    doi: 10.1371/journal.pone.0047466

    Figure Lengend Snippet: Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in G0 by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).

    Article Snippet: Cell cycle synchronization and analysis by flow cytometrySynchronization of the cells in G0 phase was achieved by culturing cells as a confluent layer for 72 h followed by serum starvation (0.2% serum) for 72 h. The cells were released from G0 by trypsination (Trypsin-EDTA 200 mg/l, Lonza) for 4 min at 37°C and cultivated in standard growth medium at 25% confluence.

    Techniques: Quantitative RT-PCR, Standard Deviation, Staining, Flow Cytometry, Blocking Assay, Isolation, Electrophoresis, Hybridization