Journal: PLOS Pathogens

Article Title: Single amino acid substitution in RdRp reduces viral recombination frequency of NADC30-like porcine reproductive and respiratory syndrome virus type 2

doi: 10.1371/journal.ppat.1014500

Figure Lengend Snippet: HeB108 and its ribavirin-associated mutants were inoculated into Marc-145 cells that were pre-treated with ribavirin (A) or 5-FU (B) at an MOI of 0.01 for 72 h. HuN4 and its ribavirin-associated mutants were inoculated into Marc-145 cells that were pre-treated with ribavirin (C) or 5-FU (D) . Cells without ribavirin or 5-FU treatment served as a control to evaluate the virus titers. Viral genomic RNA level was determined by RT-qPCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: Monolayers of Marc-145 cells were pre-treated with 100, 200, 300, and 350 μM ribavirin, or 400 and 600 μM 5-fluorouracil (5-FU) (MCE, China) for 2 h. The cells were infected with site-directed mutated or parental virus at an MOI of 0.01 for 2 h. The infected cells were washed three times with PBS.

Techniques: Control, Virus, Quantitative RT-PCR