Journal: bioRxiv

Article Title: Differential TDP-43 interactomes between the cortex and cerebellum in the mouse

doi: 10.64898/2026.01.16.699854

Figure Lengend Snippet: TDP-43 differentially interacts with core paraspeckle proteins FUS, PSPC1, SFPQ and NONO in the cortex and cerebellum (a) Representative examples of co-immunoprecipitation of FUS, PSPC1, SFPQ and NONO following TDP-43 pull-down in the cortex and cerebellum of 3-month-old C57Bl/6J mice with both the N (Abcam, #ab225710) and C (Proteintech #12892-1-AP) terminal TDP-43 antibodies. All four proteins were detected in the IP fraction of the cortex with both antibodies, with no band present with IgG alone. In contrast, PSPC1 was absent from the cerebellar samples following pull down with either antibody. IP: immunoprecipitation, FT: flow through. (b) Representative examples of western blot detection of total, nuclear and cytoplasmic FUS, PSPC1, SFPQ and NONO expression levels in the cortex and cerebellum of 3-month-old C57Bl/6J mice. (c-e) Quantification of FUS, PSPC1, SFPQ and NONO total (c), nuclear (d) and cytoplasmic (e) expression levels in the cortex and cerebellum of 3-month-old C57Bl/6J mice. PSPC1 levels were significantly reduced in the nuclear fraction of the cerebellum compared to the cortex. No other significant changes were observed. cx: cortex, cb: cerebellum, n=3 *p<0.05 Welch’s t-test

Article Snippet: Mouse anti FUS (1:500, Santa Cruz: sc-373698), rabbit anti-PSPC1 (1:1000, Abcam: ab133574), mouse anti-SFPQ (1:500, Invitrogen: MA1-25325) and rabbit anti-NONO (1:500, Abcam: ab133574) antibodies were used with loading controls and secondary antibodies as before.

Techniques: Immunoprecipitation, Western Blot, Expressing

Journal: bioRxiv

Article Title: Differential TDP-43 interactomes between the cortex and cerebellum in the mouse

doi: 10.64898/2026.01.16.699854

Figure Lengend Snippet: NONO shows differential cellular distribution between the cortex and cerebellum. (a-d) Representative examples of paraspeckle proteins (PSPr), FUS (a), PSPC1 (b), SFPQ (c) and NONO (d) distribution and colocalisation with TDP-43 in the motor cortex and Crus 1 region of the cerebellum. Scale bar is 20μm. All four paraspeckle proteins show abundant staining in the nucleus, with minimal evidence of expression within the cytoplasm with the exception of NONO within the cerebellum, which shows a distinct punctate pattern of staining within the nucleus and cytoplasm. TDP-43 shows abundant nuclear colocalisation with all four paraspeckle proteins in both the cortex and cerebellum, but no robust evidence of colocalization in the cytoplasm

Article Snippet: Mouse anti FUS (1:500, Santa Cruz: sc-373698), rabbit anti-PSPC1 (1:1000, Abcam: ab133574), mouse anti-SFPQ (1:500, Invitrogen: MA1-25325) and rabbit anti-NONO (1:500, Abcam: ab133574) antibodies were used with loading controls and secondary antibodies as before.

Techniques: Staining, Expressing

Journal: bioRxiv

Article Title: Differential TDP-43 interactomes between the cortex and cerebellum in the mouse

doi: 10.64898/2026.01.16.699854

Figure Lengend Snippet: TDP-43 interactions with FUS, PSPC1, SFPQ and NONO show distinct differences between different cortical and cerebellar cell populations (a) Representative examples of proximity ligation assay (PLA) assessment of TDP-43 with FUS, PSPC1, SFPQ and NONO. (b) Quantitative analysis of PLA spot counts in the large cells of the motor cortex (MC; layer V), and the large (Purkinje cells; PC) and small (Granule cells; GC) of the cerebellum. n=3, *p<0.05; **p<0.01 cytoplasm vs. nucleus; †p<0.05 nucleus vs. motor cortex nucleus #p<0.05; ##p<0.01 cytoplasm vs. motor cortex cytoplasm. Two-way ANOVA with Fisher LSD post hoc. (c) Quantitative analysis of mean PLA spot size in large cells of the motor cortex (MC) and large (PC) and small (GC) cells of the cerebellum. n=3, *p<0.05 vs motor cortex. One way ANOVA with Tukey post hoc

Article Snippet: Mouse anti FUS (1:500, Santa Cruz: sc-373698), rabbit anti-PSPC1 (1:1000, Abcam: ab133574), mouse anti-SFPQ (1:500, Invitrogen: MA1-25325) and rabbit anti-NONO (1:500, Abcam: ab133574) antibodies were used with loading controls and secondary antibodies as before.

Techniques: Proximity Ligation Assay

Journal: Molecular cell

Article Title: ULK1/2 Regulates Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97

doi: 10.1016/j.molcel.2019.03.027

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following primary antibodies were used: mouse anti-SQSTM1/p62 (Abnova; H00008878-M01), rabbit anti-ubiquitin (DAKO; Z0458), rabbit anti-LC3B (MBL; PM036), rat anti-LAMP1 (Developmental Studies Hybridoma Bank; 1D4B), mouse anti–SMI-31 (Covance; SMI-31R), rabbit anti–β-amyloid 1–42 (Millipore; AB5078P), rabbit anti-FUS (Bethyl Laboratories; IHC-00074), rabbit anti–TDP-43 (Proteintech; 10782–2-AP), goat anti-TIA1 (Santa Cruz Biotechnology; sc-1751), and mouse anti–hnRNP A2B1 (Santa Cruz Biotechnology; sc-53531).

Techniques: Recombinant, Western Blot, Software

Journal: Cell Reports

Article Title: Molecular Dissection of FUS Points at Synergistic Effect of Low-Complexity Domains in Toxicity

doi: 10.1016/j.celrep.2018.06.070

Figure Lengend Snippet:

Article Snippet: CMV6 FUS-FLAG WT plasmid , Origene , TP301808.

Techniques: Virus, Recombinant, Protease Inhibitor, Transgenic Assay, Plasmid Preparation