Journal: Molecular Biology and Evolution

Article Title: Horizontally Transferred DNA in the Genome of the Fungus Pyricularia oryzae is Associated With Repressive Histone Modifications

doi: 10.1093/molbev/msad186

Figure Lengend Snippet: Neighbor-joining trees of two genes in the “inserted” segments in the Br48 genome, and that of a conserved gene. ( A ) FAD/FMN-binding dehydrogenase. ( B ) Putative small secreted protein. ( C ) Elongation factor-1 gamma (the conserved gene). Accession numbers of protein sequences used in the analysis are shown after species names except sequences detected in the segment of Br48. Accession numbers of protein sequences used in the analysis are shown after species names. Bas, Basidiomycetes (shown in Brown); E, Eurotiomycetes (green); D, Dothideomycetes (Blue); L, Leothiomycetes (purple); P, Prokaryotes (red); S, Sordariomycetes (gray); Sc, Schizosaccharomyces (pink).

Article Snippet: To calculate IPC, twenty Ascomycete fungal species were selected, which included twelve Sordariomycetes fungi, Pyricularia grisea , Magnaporthiopsis poae (ATCC 64411), Gaeumannomyces tritici (R3-111a-1), Podospora comate , Colletotrichum orbiculare (MAFF 240422), Cryphonectria parasitica (EP155), Valsa mali var. pyri , Xylaria grammica , Fusarium oxysporum f. sp. cubense , Hirsutella minnesotensis (3608), Metarhizium brunneum (ARSEF 3297), and Trichoderma reesei (QM6a), three Leotiomycetes fungi, Sclerotinia sclerotiorum (1980 UF-70), Botrytis cinerea (BcDW1), and Pseudogymnoascus sp. (WSF 3629), two Eurotiomycetes fungi, Penicillium nordicum , and Aspergillus niger (ATCC 1015), and three Dothideomycetes fungi, Bipolaris maydis (ATCC 48331), Pyrenophora tritici-repentis (Pt-1C-BFP), and Alternaria atra .

Techniques: Binding Assay

Journal: Molecular Biology and Evolution

Article Title: Horizontally Transferred DNA in the Genome of the Fungus Pyricularia oryzae is Associated With Repressive Histone Modifications

doi: 10.1093/molbev/msad186

Figure Lengend Snippet: Association of silent histone marks with sequences of “movement”. ( A ) Read mapping of H3K9me3 and H3K27me3 on chromosome 6 of Br48. The black line shows moving averages of 15 consecutive IPC values on chromosome 6. The thick blue line indicates the H3K27me3-rich 110 kb region analyzed in detail (see the text). Since a part of chromosome 6 in the 70-15 strain was translocated to chromosome 1, the corresponding region in chromosome 1 was added to chromosome 6 of the strain for a comparison of genome structure. The black arrowhead indicates the connection point. ( B ) Neighbor-joining trees of PKS-NRPS hybrid protein and galactose oxidase protein in the H3K27me3-rich 110 kb region. Accession numbers of protein sequences used in the analysis are shown after species names. Bas, Basidiomycetes (shown in brown); E, Eurotiomycetes (green); D, Dothideomycetes (blue); L, Leothiomycetes (purple); P, Prokaryotes (red); S, Sordariomycetes (gray).

Article Snippet: To calculate IPC, twenty Ascomycete fungal species were selected, which included twelve Sordariomycetes fungi, Pyricularia grisea , Magnaporthiopsis poae (ATCC 64411), Gaeumannomyces tritici (R3-111a-1), Podospora comate , Colletotrichum orbiculare (MAFF 240422), Cryphonectria parasitica (EP155), Valsa mali var. pyri , Xylaria grammica , Fusarium oxysporum f. sp. cubense , Hirsutella minnesotensis (3608), Metarhizium brunneum (ARSEF 3297), and Trichoderma reesei (QM6a), three Leotiomycetes fungi, Sclerotinia sclerotiorum (1980 UF-70), Botrytis cinerea (BcDW1), and Pseudogymnoascus sp. (WSF 3629), two Eurotiomycetes fungi, Penicillium nordicum , and Aspergillus niger (ATCC 1015), and three Dothideomycetes fungi, Bipolaris maydis (ATCC 48331), Pyrenophora tritici-repentis (Pt-1C-BFP), and Alternaria atra .

Techniques: Comparison