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fth1  (ABclonal Biotechnology)
94
ABclonal Biotechnology fth1
FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, <t>FTH1</t> and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Fth1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fth1/Ferritin+Heavy+Chain+Rabbit+pAb/pmc13683295-320-25-26
Average 94 stars, based on 1 article reviews
fth1 - by Bioz Stars, 2026-11
94/100 stars
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anti fth1  (Proteintech)
96
Proteintech anti fth1
FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, <t>FTH1</t> and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Anti Fth1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fth1/ACSL4%2FFACL4+Antibody/pm42886409-144-15-17
Average 96 stars, based on 1 article reviews
anti fth1 - by Bioz Stars, 2026-11
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monoclonal anti ferritin heavy chain antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc monoclonal anti ferritin heavy chain antibody
FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, <t>FTH1</t> and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Monoclonal Anti Ferritin Heavy Chain Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fth1/FTH1+Antibody/pm42834360-114-5-13
Average 96 stars, based on 1 article reviews
monoclonal anti ferritin heavy chain antibody - by Bioz Stars, 2026-11
96/100 stars
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fth  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc fth
FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, <t>FTH1</t> and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Fth, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fth1/FTH1+Rabbit+mAb/10__1038_slash_s41598___026___65300___2-93-26-27
Average 96 stars, based on 1 article reviews
fth - by Bioz Stars, 2026-11
96/100 stars
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anti fth1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti fth1
FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, <t>FTH1</t> and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Anti Fth1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fth1/FTH1+Rabbit+mAb/pm42509425-50-42-43
Average 96 stars, based on 1 article reviews
anti fth1 - by Bioz Stars, 2026-11
96/100 stars
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FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, FTH1 and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Journal: Molecular Biomedicine

Article Title: Curcumin analogue FLLL32 induces ferroptosis by targeting the STAT3-GPX4/SLC40A1 axis in colorectal cancer

doi: 10.1186/s43556-026-00575-5

Figure Lengend Snippet: FLLL32 induces a Ferrostatin-1-rescuable ferroptotic response in CRC cells. a Cell viability of HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Z-VAD-FMK or Ferrostatin-1. b , c RT-qPCR analysis of SLC40A1 and GPX4, FTH1 and FTL mRNA expression in HCT116 cells treated with DMSO or FLLL32. d Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. e DCF fluorescence analysis showing ROS levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. f FerroOrange fluorescence analysis showing intracellular Fe 2+ levels in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. g Colony formation of HCT116 and HT29 cells treated with Ferrostatin-1 and FLLL32. h Quantification of colony numbers in HCT116 and HT29 cells. i Western blot analysis of FTH1, FTL, GPX4 and SLC40A1 in HCT116 and HT29 cells treated with FLLL32 in the presence or absence of Ferrostatin-1. β-Tubulin was used as the loading control. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Primary antibodies used for immunoblot analysis were as follows: β-Tubulin (ZSGB-BIO, TA347064), STAT3 (HUABIO, ET1603-40), Phospho-STAT3 (Tyr705) (CST, 9145S), GPX4 (HUABIO, ET1706-45), SLC40A1 (CST, 80672T), FTH1 (Abclonal, A1144), FTL (Abclonal, A1768), Lamin B1 (Abclonal, A11495), E-cadherin (Proteintech, 20874-1-AP), N-cadherin (Proteintech, 22018-1-AP), Vimentin (Proteintech, 10366-1-AP), and HA-tag (Proteintech, 66006-2-Ig).

Techniques: Quantitative RT-PCR, Analysis, Expressing, Western Blot, Control, Fluorescence, Colony Assay