freestyle 293 expression system  (Thermo Fisher)


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    Name:
    FreeStyle 293 Expression System
    Description:
    The FreeStyle 293 Expression System is a complete suspension cell culture system for generating large amounts of mammalian recombinant protein Free your lab from the time consuming labor intensive process of seeding feeding passing and maintaining multiple flasks of adherent cells Figure 1 The FreeStyle 293 Expression System combines GIBCO FreeStyle 293 Expression Medium with 293fectin a cationic lipid formulation designed to transfect suspension FreeStyle 293 F cells at high efficiency FreeStyle 293 F cells and 293fectin are available as part of the Expression System or separately FreeStyle 293 Expression Medium is chemically defined protein free medium specifically developed for the ability to support the growth and transfection of 293 F cells under suspension type culture conditions Figure 2 The medium is a complete ready to use medium that has been supplemented with GlutaMAX I Supplement and is animal origin free FreeStyle 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum free conditions
    Catalog Number:
    k900001
    Price:
    None
    Applications:
    Bioproduction|Cell Culture|Mammalian Expression|Mammalian Protein Production|Plasmid Transfection|Protein Biology|Protein Expression|Transient Protein Production & High-Throughput Screening|Antibody & Cell Culture Production|Transfection
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    Kits and Assays
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    Structured Review

    Thermo Fisher freestyle 293 expression system
    The FreeStyle 293 Expression System is a complete suspension cell culture system for generating large amounts of mammalian recombinant protein Free your lab from the time consuming labor intensive process of seeding feeding passing and maintaining multiple flasks of adherent cells Figure 1 The FreeStyle 293 Expression System combines GIBCO FreeStyle 293 Expression Medium with 293fectin a cationic lipid formulation designed to transfect suspension FreeStyle 293 F cells at high efficiency FreeStyle 293 F cells and 293fectin are available as part of the Expression System or separately FreeStyle 293 Expression Medium is chemically defined protein free medium specifically developed for the ability to support the growth and transfection of 293 F cells under suspension type culture conditions Figure 2 The medium is a complete ready to use medium that has been supplemented with GlutaMAX I Supplement and is animal origin free FreeStyle 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum free conditions
    https://www.bioz.com/result/freestyle 293 expression system/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    freestyle 293 expression system - by Bioz Stars, 2020-08
    99/100 stars

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    Produced:

    Article Title: Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice
    Article Snippet: .. Transient expression of chimeric antibodies and purification Chimeric antibodies were expressed using the FreeStyle™ 293 expression system (Invitrogen) to obtain antibodies produced in a defined, serum-free medium. .. Constructs encoding chimeric IgG1 were transfected into 293-F cells by use of 293 fectin (Invitrogen).

    Transfection:

    Article Title: Crystal Structure of the Human Cannabinoid Receptor CB1
    Article Snippet: .. CB1 -Flavodoxin construct was transfected and expressed in HEK293F cells (Invitrogen) (Passage number is 12–20) using the FreeStyleTM 293 Expression system (Invitrogen). .. Briefly, HEK293F cells were seeded on day 0 at 6×105 cells/ml in freeStyle 293 expression medium (Invitrogen).

    Article Title: Optimization of HEK 293 cell growth by addition of non-animal derived components using design of experiments
    Article Snippet: .. The cells grow to a maximum concentration of ~ 3×106 cells/ml with over 90% viability and show an average doubling time of 24 h. In addition, HEK 293 cell growth was assessed in two other commercial serum-free culture media, namely ExCell 293 from SAFC Biosciences (Hampshire, UK) and Freestyle 293 from Invitrogen (Carlsbad, CA, USA) both compatible with PEI-mediated transient transfection , showing similar results (Figure ). ..

    Construct:

    Article Title: Crystal Structure of the Human Cannabinoid Receptor CB1
    Article Snippet: .. CB1 -Flavodoxin construct was transfected and expressed in HEK293F cells (Invitrogen) (Passage number is 12–20) using the FreeStyleTM 293 Expression system (Invitrogen). .. Briefly, HEK293F cells were seeded on day 0 at 6×105 cells/ml in freeStyle 293 expression medium (Invitrogen).

    Purification:

    Article Title: Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice
    Article Snippet: .. Transient expression of chimeric antibodies and purification Chimeric antibodies were expressed using the FreeStyle™ 293 expression system (Invitrogen) to obtain antibodies produced in a defined, serum-free medium. .. Constructs encoding chimeric IgG1 were transfected into 293-F cells by use of 293 fectin (Invitrogen).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
    Article Snippet: .. Materials The human prostate cancer (PC-3) cell line was purchased from ATCC (Rockville, MD), the FreeStyle™ 293 expression medium, Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) and 0.25% Trypsin-EDTA were obtained from Gibco-Invitrogen (Carlsbad, CA), the RQ1 RNase-free DNase and the CellTiter-Glo® Luminescent Cell Viability Assay kit were obtained from Promega, (Madison, WI), the DHL™ Cell Cytotoxicity Assay kit was from Anaspec (San Jose, CA) and the ELISA MAX™ Set Deluxe kit for human IL-8 was obtained from BioLegend (San Diego, CA). .. The in situ cell death detection kit [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)] was purchased from Roche (Indianapolis, IN), the DNase-free RNase A was obtained from Qiagen (Valencia, CA), Precast Tris-HCl gradient Ready gels® were from BioRad (Richmond, CA) and [3 H]thymidine (6.7 Ci/mmol) was from ICN (Irvine, CA).

    Concentration Assay:

    Article Title: Optimization of HEK 293 cell growth by addition of non-animal derived components using design of experiments
    Article Snippet: .. The cells grow to a maximum concentration of ~ 3×106 cells/ml with over 90% viability and show an average doubling time of 24 h. In addition, HEK 293 cell growth was assessed in two other commercial serum-free culture media, namely ExCell 293 from SAFC Biosciences (Hampshire, UK) and Freestyle 293 from Invitrogen (Carlsbad, CA, USA) both compatible with PEI-mediated transient transfection , showing similar results (Figure ). ..

    Cell Culture:

    Article Title: Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation
    Article Snippet: .. HEK-293F cells were cultured in FreeStyle™ 293 Expression Medium (Invitrogen). .. Plasmids were transfected into HEK-293F cells using 293fectin™ according to manufacturer's instructions (Invitrogen).

    Article Title: Identification of near pan-neutralizing antibodies against HIV-1 by deconvolution of plasma humoral responses
    Article Snippet: .. FreeStyle™ 293-F Cells (sex: female) were obtained from Thermo Fisher Scientific (Waltham, MA) and were cultured in 5% C02 at 37°C in FreeStyle™ 293 Expression Medium from Gibco (Gaithersburg, MD). .. Recombinant HIV-1 antigens were generated as described previously (31).

    Expressing:

    Article Title: Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation
    Article Snippet: .. HEK-293F cells were cultured in FreeStyle™ 293 Expression Medium (Invitrogen). .. Plasmids were transfected into HEK-293F cells using 293fectin™ according to manufacturer's instructions (Invitrogen).

    Article Title: Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice
    Article Snippet: .. Transient expression of chimeric antibodies and purification Chimeric antibodies were expressed using the FreeStyle™ 293 expression system (Invitrogen) to obtain antibodies produced in a defined, serum-free medium. .. Constructs encoding chimeric IgG1 were transfected into 293-F cells by use of 293 fectin (Invitrogen).

    Article Title: Dissection of Epitope-Specific Mechanisms of Neutralization of Influenza Virus by Intact IgG and Fab Fragments
    Article Snippet: .. nAb HC19 was transiently expressed by using the FreeStyle 293F expression system (Life Technologies), while FI6v3 was constitutively expressed from a stable HEK293F cell line. .. Plasmids encoding HC19 IgG and the stable cell line expressing FI6v3 IgG were kindly provided by Jesse Bloom at Fred Hutchinson Cancer Research Center (Seattle, WA).

    Article Title: Identification of near pan-neutralizing antibodies against HIV-1 by deconvolution of plasma humoral responses
    Article Snippet: .. FreeStyle™ 293-F Cells (sex: female) were obtained from Thermo Fisher Scientific (Waltham, MA) and were cultured in 5% C02 at 37°C in FreeStyle™ 293 Expression Medium from Gibco (Gaithersburg, MD). .. Recombinant HIV-1 antigens were generated as described previously (31).

    Article Title: Crystal Structure of the Human Cannabinoid Receptor CB1
    Article Snippet: .. CB1 -Flavodoxin construct was transfected and expressed in HEK293F cells (Invitrogen) (Passage number is 12–20) using the FreeStyleTM 293 Expression system (Invitrogen). .. Briefly, HEK293F cells were seeded on day 0 at 6×105 cells/ml in freeStyle 293 expression medium (Invitrogen).

    Article Title: Reformatting palivizumab and motavizumab from IgG to human IgA impairs their efficacy against RSV infection in vitro and in vivo
    Article Snippet: .. FreeStyle™ 293-F cells (Invitrogen) were maintained in FreeStyle™ 293 Expression Medium (Invitrogen) at 37°C, 8% CO2 in an orbital shaker (125 rpm). .. HEp-2 cells (ATCC) were maintained in IMDM (Gibco) supplemented with penicillin (100 Units/mL; Life Technologies), streptomycin (100 µg/mL; Life Technologies) and 10% fetal calf serum (FCS) at 37°C, 5% CO2 .

    Article Title: Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
    Article Snippet: .. Materials The human prostate cancer (PC-3) cell line was purchased from ATCC (Rockville, MD), the FreeStyle™ 293 expression medium, Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) and 0.25% Trypsin-EDTA were obtained from Gibco-Invitrogen (Carlsbad, CA), the RQ1 RNase-free DNase and the CellTiter-Glo® Luminescent Cell Viability Assay kit were obtained from Promega, (Madison, WI), the DHL™ Cell Cytotoxicity Assay kit was from Anaspec (San Jose, CA) and the ELISA MAX™ Set Deluxe kit for human IL-8 was obtained from BioLegend (San Diego, CA). .. The in situ cell death detection kit [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)] was purchased from Roche (Indianapolis, IN), the DNase-free RNase A was obtained from Qiagen (Valencia, CA), Precast Tris-HCl gradient Ready gels® were from BioRad (Richmond, CA) and [3 H]thymidine (6.7 Ci/mmol) was from ICN (Irvine, CA).

    Modification:

    Article Title: Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
    Article Snippet: .. Materials The human prostate cancer (PC-3) cell line was purchased from ATCC (Rockville, MD), the FreeStyle™ 293 expression medium, Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) and 0.25% Trypsin-EDTA were obtained from Gibco-Invitrogen (Carlsbad, CA), the RQ1 RNase-free DNase and the CellTiter-Glo® Luminescent Cell Viability Assay kit were obtained from Promega, (Madison, WI), the DHL™ Cell Cytotoxicity Assay kit was from Anaspec (San Jose, CA) and the ELISA MAX™ Set Deluxe kit for human IL-8 was obtained from BioLegend (San Diego, CA). .. The in situ cell death detection kit [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)] was purchased from Roche (Indianapolis, IN), the DNase-free RNase A was obtained from Qiagen (Valencia, CA), Precast Tris-HCl gradient Ready gels® were from BioRad (Richmond, CA) and [3 H]thymidine (6.7 Ci/mmol) was from ICN (Irvine, CA).

    Cell Viability Assay:

    Article Title: Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
    Article Snippet: .. Materials The human prostate cancer (PC-3) cell line was purchased from ATCC (Rockville, MD), the FreeStyle™ 293 expression medium, Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) and 0.25% Trypsin-EDTA were obtained from Gibco-Invitrogen (Carlsbad, CA), the RQ1 RNase-free DNase and the CellTiter-Glo® Luminescent Cell Viability Assay kit were obtained from Promega, (Madison, WI), the DHL™ Cell Cytotoxicity Assay kit was from Anaspec (San Jose, CA) and the ELISA MAX™ Set Deluxe kit for human IL-8 was obtained from BioLegend (San Diego, CA). .. The in situ cell death detection kit [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)] was purchased from Roche (Indianapolis, IN), the DNase-free RNase A was obtained from Qiagen (Valencia, CA), Precast Tris-HCl gradient Ready gels® were from BioRad (Richmond, CA) and [3 H]thymidine (6.7 Ci/mmol) was from ICN (Irvine, CA).

    Cytotoxicity Assay:

    Article Title: Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
    Article Snippet: .. Materials The human prostate cancer (PC-3) cell line was purchased from ATCC (Rockville, MD), the FreeStyle™ 293 expression medium, Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) and 0.25% Trypsin-EDTA were obtained from Gibco-Invitrogen (Carlsbad, CA), the RQ1 RNase-free DNase and the CellTiter-Glo® Luminescent Cell Viability Assay kit were obtained from Promega, (Madison, WI), the DHL™ Cell Cytotoxicity Assay kit was from Anaspec (San Jose, CA) and the ELISA MAX™ Set Deluxe kit for human IL-8 was obtained from BioLegend (San Diego, CA). .. The in situ cell death detection kit [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)] was purchased from Roche (Indianapolis, IN), the DNase-free RNase A was obtained from Qiagen (Valencia, CA), Precast Tris-HCl gradient Ready gels® were from BioRad (Richmond, CA) and [3 H]thymidine (6.7 Ci/mmol) was from ICN (Irvine, CA).

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    Thermo Fisher freestyle 293 expression medium
    Engineering the frequency of glycosylation sites in the Muc1 polymer backbone tunes O-glycan maturation. (a) Components and features of secreted Muc1 and engineered variants each with 21 tandem repeats. (b) Tandem repeat sequences of secreted mucin mutants and the molecular weight of the polypeptide backbones. Single, double, and triple glycosylation mutants (sMuc1S, sMuc1D, and sMuc1T) have one, two, or three, serine/threonine (S/T) to alanine substitutions per repeat, respectively. (c) Representative Western blot analysis of affinity-purified recombinant secreted mucins from FreeStyle 293-F cell culture media probed with anti-SUMOstar antibody and PNA, s-WGA, and VVA lectins (of three independent experiments). The lectin blot was costained in multiple colors with PNA-Alexa Fluor 568, s-WGA-FITC, and biotinylated VVA (Secondary: NeutrAvidin-Dylight 650). (d) Representative fluorescence intensity electrophoretograms of the blots in part c. (e) Ratiometric intensity analysis of PNA to VVA signal (upper) and s-WGA to VVA signal (lower) for the indicated mucins and their corresponding frequency of S/T glycosylation sites in the polymer backbone. Ratiometric fluorescence intensity was quantified along each lane and normalized to the signal from the secreted mucin with wild-type Muc1 tandem repeats (sMuc1); data presented as the mean and SEM from at least three independent experiments. * P
    Freestyle 293 Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/freestyle 293 expression medium/product/Thermo Fisher
    Average 99 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    freestyle 293 expression medium - by Bioz Stars, 2020-08
    99/100 stars
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    Engineering the frequency of glycosylation sites in the Muc1 polymer backbone tunes O-glycan maturation. (a) Components and features of secreted Muc1 and engineered variants each with 21 tandem repeats. (b) Tandem repeat sequences of secreted mucin mutants and the molecular weight of the polypeptide backbones. Single, double, and triple glycosylation mutants (sMuc1S, sMuc1D, and sMuc1T) have one, two, or three, serine/threonine (S/T) to alanine substitutions per repeat, respectively. (c) Representative Western blot analysis of affinity-purified recombinant secreted mucins from FreeStyle 293-F cell culture media probed with anti-SUMOstar antibody and PNA, s-WGA, and VVA lectins (of three independent experiments). The lectin blot was costained in multiple colors with PNA-Alexa Fluor 568, s-WGA-FITC, and biotinylated VVA (Secondary: NeutrAvidin-Dylight 650). (d) Representative fluorescence intensity electrophoretograms of the blots in part c. (e) Ratiometric intensity analysis of PNA to VVA signal (upper) and s-WGA to VVA signal (lower) for the indicated mucins and their corresponding frequency of S/T glycosylation sites in the polymer backbone. Ratiometric fluorescence intensity was quantified along each lane and normalized to the signal from the secreted mucin with wild-type Muc1 tandem repeats (sMuc1); data presented as the mean and SEM from at least three independent experiments. * P

    Journal: ACS synthetic biology

    Article Title: Sequence-Specific Mucins for Glycocalyx Engineering

    doi: 10.1021/acssynbio.9b00127

    Figure Lengend Snippet: Engineering the frequency of glycosylation sites in the Muc1 polymer backbone tunes O-glycan maturation. (a) Components and features of secreted Muc1 and engineered variants each with 21 tandem repeats. (b) Tandem repeat sequences of secreted mucin mutants and the molecular weight of the polypeptide backbones. Single, double, and triple glycosylation mutants (sMuc1S, sMuc1D, and sMuc1T) have one, two, or three, serine/threonine (S/T) to alanine substitutions per repeat, respectively. (c) Representative Western blot analysis of affinity-purified recombinant secreted mucins from FreeStyle 293-F cell culture media probed with anti-SUMOstar antibody and PNA, s-WGA, and VVA lectins (of three independent experiments). The lectin blot was costained in multiple colors with PNA-Alexa Fluor 568, s-WGA-FITC, and biotinylated VVA (Secondary: NeutrAvidin-Dylight 650). (d) Representative fluorescence intensity electrophoretograms of the blots in part c. (e) Ratiometric intensity analysis of PNA to VVA signal (upper) and s-WGA to VVA signal (lower) for the indicated mucins and their corresponding frequency of S/T glycosylation sites in the polymer backbone. Ratiometric fluorescence intensity was quantified along each lane and normalized to the signal from the secreted mucin with wild-type Muc1 tandem repeats (sMuc1); data presented as the mean and SEM from at least three independent experiments. * P

    Article Snippet: FreeStyle 293-F cells were cultured in suspension in FreeStyle 293 Expression Medium (Thermo-Fisher).

    Techniques: Molecular Weight, Western Blot, Affinity Purification, Cell Culture, Whole Genome Amplification, Fluorescence

    Staining of different CSCs-markers on 3D spheres derived from unsorted Caki1-cells. 3D spheres were grown in FreeStyle™ 293 and stained for CSCs-markers  (A)  CD44,  (C)  CD105,  (E)  CXCR4, and  (G)  CD133. 3D spheres were stained with primary antibody (e.g. CD44, CD105, CXCR4 and CD133) + secondary antibody Alexa Fluor 488 (green) and counter-stained with the nuclear dye DAPI (blue). Positive spheres were shown as green. Merged fluorescence of Alexa Fluor 488 and DAPI is shown in  (B), (D), (F), (H) . Scale bar = 100 μm.

    Journal: Scientific Reports

    Article Title: Renal carcinoma CD105−/CD44− cells display stem-like properties in vitro and form aggressive tumors in vivo

    doi: 10.1038/s41598-020-62205-6

    Figure Lengend Snippet: Staining of different CSCs-markers on 3D spheres derived from unsorted Caki1-cells. 3D spheres were grown in FreeStyle™ 293 and stained for CSCs-markers (A) CD44, (C) CD105, (E) CXCR4, and (G) CD133. 3D spheres were stained with primary antibody (e.g. CD44, CD105, CXCR4 and CD133) + secondary antibody Alexa Fluor 488 (green) and counter-stained with the nuclear dye DAPI (blue). Positive spheres were shown as green. Merged fluorescence of Alexa Fluor 488 and DAPI is shown in (B), (D), (F), (H) . Scale bar = 100 μm.

    Article Snippet: Sorted CSCs/TICs (CD105+/−, CD133+/−, CXCR4+/−, and CD44+/−) cells were cultured in FreeStyle 293 Expression Medium (ThermoFisher Scientific, Massachusetts, USA).

    Techniques: Staining, Derivative Assay, Fluorescence

    Sorting of CSCs based on FACS analysis by FACS Aria II cell sorter. Dot plots  (A) (C)  and  (E)  showing unstained cells before FACS sorting and dot plots showing stained cells positive for  (B)  CD105−CSCs  (D)  CD44−CSCs and  (F)  CD133-CSCs from Caki-1 cell line. These CSCs were sorted and culture in normoxic condition in FreeStyle™ 293 medium before xenograft experiments. The percentage of sorted cells positive for CD105, CD44, and CD133 makers were approximately 9%, 96% and 1%, respectively.

    Journal: Scientific Reports

    Article Title: Renal carcinoma CD105−/CD44− cells display stem-like properties in vitro and form aggressive tumors in vivo

    doi: 10.1038/s41598-020-62205-6

    Figure Lengend Snippet: Sorting of CSCs based on FACS analysis by FACS Aria II cell sorter. Dot plots (A) (C) and (E) showing unstained cells before FACS sorting and dot plots showing stained cells positive for (B) CD105−CSCs (D) CD44−CSCs and (F) CD133-CSCs from Caki-1 cell line. These CSCs were sorted and culture in normoxic condition in FreeStyle™ 293 medium before xenograft experiments. The percentage of sorted cells positive for CD105, CD44, and CD133 makers were approximately 9%, 96% and 1%, respectively.

    Article Snippet: Sorted CSCs/TICs (CD105+/−, CD133+/−, CXCR4+/−, and CD44+/−) cells were cultured in FreeStyle 293 Expression Medium (ThermoFisher Scientific, Massachusetts, USA).

    Techniques: FACS, Staining

    Generation of 3D spheres derived from unsorted Caki-1 cells in FreeStyle™ 293 and RPMI-1640+10%FBS medium. Representative pictures of 3D spheres formed by Caki-1 cells in  (A)  FreeStyle™ 293 and  (B)  RPMI-1640+10%FBS. Caki-1 cells cultured in low-attachment plate tend to attach more in RPMI-1640+10%FBS media in comparison to FreeStyle™ 293.  (C)  Quantitative analysis of 3D spheres formation in FreeStyle™ 293 and RPMI-1640+10%FBS medium. Scale bar = 100 μm and *** P

    Journal: Scientific Reports

    Article Title: Renal carcinoma CD105−/CD44− cells display stem-like properties in vitro and form aggressive tumors in vivo

    doi: 10.1038/s41598-020-62205-6

    Figure Lengend Snippet: Generation of 3D spheres derived from unsorted Caki-1 cells in FreeStyle™ 293 and RPMI-1640+10%FBS medium. Representative pictures of 3D spheres formed by Caki-1 cells in (A) FreeStyle™ 293 and (B) RPMI-1640+10%FBS. Caki-1 cells cultured in low-attachment plate tend to attach more in RPMI-1640+10%FBS media in comparison to FreeStyle™ 293. (C) Quantitative analysis of 3D spheres formation in FreeStyle™ 293 and RPMI-1640+10%FBS medium. Scale bar = 100 μm and *** P

    Article Snippet: Sorted CSCs/TICs (CD105+/−, CD133+/−, CXCR4+/−, and CD44+/−) cells were cultured in FreeStyle 293 Expression Medium (ThermoFisher Scientific, Massachusetts, USA).

    Techniques: Derivative Assay, Cell Culture