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Illumina Inc fragmentation buffer
Fragmentation Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fragmentation buffer/product/Illumina Inc
Average 92 stars, based on 62 article reviews
Price from $9.99 to $1999.99
fragmentation buffer - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Magnetic Beads:

Article Title: Recurrent SRF-RELA Fusions Define a Novel Subset of Cellular Variants in the Myofibroma/Myopericytoma Spectrum: A Potential Diagnostic Pitfall with Sarcomas with Myogenic Differentiation
Article Snippet: .. Total RNA was extracted from frozen tissue from 2 index cases using RNeasy Plus Mini (Qiagen), followed by mRNA isolation with oligo(dT) magnetic beads and fragmentation by incubation at 94°C in fragmentation buffer (Illumina) for 2.5 minutes. .. After gel size-selection (350–400bp) and adapter ligation, the library was enriched by PCR for 15 cycles and purified.

Article Title: EWSR1 fusions with CREB family transcription factors define a novel myxoid mesenchymal tumor with predilection for intracranial location
Article Snippet: .. Total RNA was extracted from frozen tissue the index case #1 using RNeasy Plus Mini (Qiagen), followed by mRNA isolation with oligo(dT) magnetic beads and fragmentation by incubation at 94°C in fragmentation buffer (Illumina) for 2.5 minutes. .. After gel size-selection (350–400bp) and adapter ligation, the library was enriched by PCR for 15 cycles and purified.

Isolation:

Article Title: Recurrent SRF-RELA Fusions Define a Novel Subset of Cellular Variants in the Myofibroma/Myopericytoma Spectrum: A Potential Diagnostic Pitfall with Sarcomas with Myogenic Differentiation
Article Snippet: .. Total RNA was extracted from frozen tissue from 2 index cases using RNeasy Plus Mini (Qiagen), followed by mRNA isolation with oligo(dT) magnetic beads and fragmentation by incubation at 94°C in fragmentation buffer (Illumina) for 2.5 minutes. .. After gel size-selection (350–400bp) and adapter ligation, the library was enriched by PCR for 15 cycles and purified.

Article Title: EWSR1 fusions with CREB family transcription factors define a novel myxoid mesenchymal tumor with predilection for intracranial location
Article Snippet: .. Total RNA was extracted from frozen tissue the index case #1 using RNeasy Plus Mini (Qiagen), followed by mRNA isolation with oligo(dT) magnetic beads and fragmentation by incubation at 94°C in fragmentation buffer (Illumina) for 2.5 minutes. .. After gel size-selection (350–400bp) and adapter ligation, the library was enriched by PCR for 15 cycles and purified.

Next-Generation Sequencing:

Article Title: Abundance and distribution of sylvatic dengue virus vectors in three different land cover types in Sarawak, Malaysian Borneo
Article Snippet: .. Next-generation sequencing to detect viruses RNA (0.1–0.2 μg) quantified by NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Pittsburgh, Pennsylvania, USA), was fragmented by incubation at 94 °C for 8 min in 19.5 μl of fragmentation buffer (Illumina, San Diego, California, USA). .. Following fragmentation, first and second strand synthesis, adapter ligation and amplification of the library were performed using the TruSeq RNA Library Prep Kit v2 under conditions prescribed by the manufacturer (Illumina).

Synthesized:

Article Title: Transcriptomic analysis reveals the differentially expressed genes and pathways involved in drought tolerance in pearl millet [Pennisetum glaucum (L.) R. Br]
Article Snippet: .. The mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized using mRNA template and random hexamers, after which a second strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. .. After terminal repair and sequencing adapter ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment.

Spectrophotometry:

Article Title: Abundance and distribution of sylvatic dengue virus vectors in three different land cover types in Sarawak, Malaysian Borneo
Article Snippet: .. Next-generation sequencing to detect viruses RNA (0.1–0.2 μg) quantified by NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Pittsburgh, Pennsylvania, USA), was fragmented by incubation at 94 °C for 8 min in 19.5 μl of fragmentation buffer (Illumina, San Diego, California, USA). .. Following fragmentation, first and second strand synthesis, adapter ligation and amplification of the library were performed using the TruSeq RNA Library Prep Kit v2 under conditions prescribed by the manufacturer (Illumina).

Incubation:

Article Title: Recurrent NCOA2 gene rearrangements in congenital/infantile spindle cell rhabdomyosarcoma
Article Snippet: .. The mRNA was fragmented by incubation at 94°C for 2.5 min in fragmentation buffer (Illumina). .. To reduce the inclusion of artifact chimeric transcripts into the sequencing library, an additional gel size-selection step was introduced prior to the adapter ligation step( ).

Article Title: A Global Genomic Characterization of Nairoviruses Identifies Nine Discrete Genogroups with Distinctive Structural Characteristics and Host-Vector Associations
Article Snippet: .. Viral RNA (∼0.9 μg) was fragmented by incubation at 94°C for 8 minutes in 19.5 μL of fragmentation buffer (Illumina 15016648). .. A sequencing library was prepared from the RNA sample using an Illumina TruSeq RNA v2 kit (Illumina, San Diego, CA) following the manufacturer's protocol.

Article Title: Recurrent SRF-RELA Fusions Define a Novel Subset of Cellular Variants in the Myofibroma/Myopericytoma Spectrum: A Potential Diagnostic Pitfall with Sarcomas with Myogenic Differentiation
Article Snippet: .. Total RNA was extracted from frozen tissue from 2 index cases using RNeasy Plus Mini (Qiagen), followed by mRNA isolation with oligo(dT) magnetic beads and fragmentation by incubation at 94°C in fragmentation buffer (Illumina) for 2.5 minutes. .. After gel size-selection (350–400bp) and adapter ligation, the library was enriched by PCR for 15 cycles and purified.

Article Title: Frequent FOS Gene Rearrangements in Epithelioid Hemangioma: A Molecular Study of 58 Cases with Morphologic Reappraisal
Article Snippet: .. The mRNA was fragmented by incubation at 94°C for 2.5 min in fragmentation buffer (Illumina). .. To reduce the inclusion of artifactual chimeric transcripts due to random priming of transcript fragments into the sequencing library because of inefficient A-tailing reactions that lead to self ligation of blunt-ended template molecules , an additional gel size-selection step (capturing 350-400 bp) was introduced prior to the adapter ligation step.

Article Title: EWSR1 fusions with CREB family transcription factors define a novel myxoid mesenchymal tumor with predilection for intracranial location
Article Snippet: .. Total RNA was extracted from frozen tissue the index case #1 using RNeasy Plus Mini (Qiagen), followed by mRNA isolation with oligo(dT) magnetic beads and fragmentation by incubation at 94°C in fragmentation buffer (Illumina) for 2.5 minutes. .. After gel size-selection (350–400bp) and adapter ligation, the library was enriched by PCR for 15 cycles and purified.

Article Title: Abundance and distribution of sylvatic dengue virus vectors in three different land cover types in Sarawak, Malaysian Borneo
Article Snippet: .. Next-generation sequencing to detect viruses RNA (0.1–0.2 μg) quantified by NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Pittsburgh, Pennsylvania, USA), was fragmented by incubation at 94 °C for 8 min in 19.5 μl of fragmentation buffer (Illumina, San Diego, California, USA). .. Following fragmentation, first and second strand synthesis, adapter ligation and amplification of the library were performed using the TruSeq RNA Library Prep Kit v2 under conditions prescribed by the manufacturer (Illumina).

Article Title: Molecular Characterisation of Chikungunya Virus Infections in Trinidad and Comparison of Clinical and Laboratory Features with Dengue and Other Acute Febrile Cases
Article Snippet: .. Illumina sequencing For ten samples with low Ct values in RT-qPCR assays, viral RNA was extracted then fragmented by incubation at 94°C for eight (8) minutes in 19.5 μl of fragmentation buffer (Illumina Inc., San Diego, CA). .. First and second strand synthesis, adapter ligation and amplification of the library were performed using the Illumina TruSeq RNA Sample Preparation kit v2 under conditions prescribed by the manufacturer (Illumina Inc., San Diego, CA).

Quantitative RT-PCR:

Article Title: Molecular Characterisation of Chikungunya Virus Infections in Trinidad and Comparison of Clinical and Laboratory Features with Dengue and Other Acute Febrile Cases
Article Snippet: .. Illumina sequencing For ten samples with low Ct values in RT-qPCR assays, viral RNA was extracted then fragmented by incubation at 94°C for eight (8) minutes in 19.5 μl of fragmentation buffer (Illumina Inc., San Diego, CA). .. First and second strand synthesis, adapter ligation and amplification of the library were performed using the Illumina TruSeq RNA Sample Preparation kit v2 under conditions prescribed by the manufacturer (Illumina Inc., San Diego, CA).

Sequencing:

Article Title: Molecular Characterisation of Chikungunya Virus Infections in Trinidad and Comparison of Clinical and Laboratory Features with Dengue and Other Acute Febrile Cases
Article Snippet: .. Illumina sequencing For ten samples with low Ct values in RT-qPCR assays, viral RNA was extracted then fragmented by incubation at 94°C for eight (8) minutes in 19.5 μl of fragmentation buffer (Illumina Inc., San Diego, CA). .. First and second strand synthesis, adapter ligation and amplification of the library were performed using the Illumina TruSeq RNA Sample Preparation kit v2 under conditions prescribed by the manufacturer (Illumina Inc., San Diego, CA).

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    Illumina Inc illumina fragmentation buffer
    Workflow of <t>Illumina</t> deep sequencing and bioinformatic analyses. It includes sample preparation, cDNA library construction, Illumina sequencing, and data analyses including transcriptome assembly, BLAST search, GO annotation, and gene expression analysis.
    Illumina Fragmentation Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina fragmentation buffer/product/Illumina Inc
    Average 90 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    illumina fragmentation buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    Workflow of Illumina deep sequencing and bioinformatic analyses. It includes sample preparation, cDNA library construction, Illumina sequencing, and data analyses including transcriptome assembly, BLAST search, GO annotation, and gene expression analysis.

    Journal: Scientific Reports

    Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity

    doi: 10.1038/srep09836

    Figure Lengend Snippet: Workflow of Illumina deep sequencing and bioinformatic analyses. It includes sample preparation, cDNA library construction, Illumina sequencing, and data analyses including transcriptome assembly, BLAST search, GO annotation, and gene expression analysis.

    Article Snippet: Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

    Techniques: Sequencing, Sample Prep, cDNA Library Assay, Expressing