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Waters Corporation fractions hplc analysis
High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus <t>UPE</t> extract and <t>HPLC</t> analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.
Fractions Hplc Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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Article Title: Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction

Journal: Molecules

doi: 10.3390/molecules23123353

High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus UPE extract and HPLC analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.
Figure Legend Snippet: High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus UPE extract and HPLC analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.

Techniques Used: High Speed Counter-current Chromatography, High Performance Liquid Chromatography, Flow Cytometry

HPLC chromatograms of the extraction samples by ultrasonic ( A ), heat reflux extraction ( B ), and UPE ( C ) from Ligustri Lucidi Fructus. HPLC conditions: column, Purospher STAR RP18 Endiapped (250 mm × 4.6 mm i.d., 5 μm); mobile phase, acetonitrile ( A )-water ( B ), 0–2 min, 5–5%A; 2–40 min, 5–30%A; 40–45 min, 30–30%A; 45–53 min, 30–50%A; column temperature, 25 °C; flow rate, 1.0 mL·min −1 ; UV detection wavelength, 282 nm; injection volume, 10 μL; peak 1, salidroside; peak 2, echinacoside; peak 3, acteoside; peak 4, specnuezhenide; peak 5, isonuezhenide; and peak 6, nuezhenoside G13.
Figure Legend Snippet: HPLC chromatograms of the extraction samples by ultrasonic ( A ), heat reflux extraction ( B ), and UPE ( C ) from Ligustri Lucidi Fructus. HPLC conditions: column, Purospher STAR RP18 Endiapped (250 mm × 4.6 mm i.d., 5 μm); mobile phase, acetonitrile ( A )-water ( B ), 0–2 min, 5–5%A; 2–40 min, 5–30%A; 40–45 min, 30–30%A; 45–53 min, 30–50%A; column temperature, 25 °C; flow rate, 1.0 mL·min −1 ; UV detection wavelength, 282 nm; injection volume, 10 μL; peak 1, salidroside; peak 2, echinacoside; peak 3, acteoside; peak 4, specnuezhenide; peak 5, isonuezhenide; and peak 6, nuezhenoside G13.

Techniques Used: High Performance Liquid Chromatography, Reflux, Flow Cytometry, Injection

Related Articles

High Performance Liquid Chromatography:

Article Title: Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction
Article Snippet: .. HPLC Analysis and Identification of the Fractions HPLC analysis of the crude sample by UPE and fractions obtained from the HSCCC separation were performed on a Waters e2695 apparatus. .. Acetonitrile (A)-water (B) was appointed as the mobile phase, and the gradient elution was: 0–2 min, 5–5% A; 2–40 min, 5–30% A; 40–45 min, 30–30% A; 45–53 min, 30–50% A, at a flow rate of 1.0 mL/min.

High Speed Counter-current Chromatography:

Article Title: Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction
Article Snippet: .. HPLC Analysis and Identification of the Fractions HPLC analysis of the crude sample by UPE and fractions obtained from the HSCCC separation were performed on a Waters e2695 apparatus. .. Acetonitrile (A)-water (B) was appointed as the mobile phase, and the gradient elution was: 0–2 min, 5–5% A; 2–40 min, 5–30% A; 40–45 min, 30–30% A; 45–53 min, 30–50% A, at a flow rate of 1.0 mL/min.

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    Waters Corporation fractions hplc analysis
    High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus <t>UPE</t> extract and <t>HPLC</t> analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.
    Fractions Hplc Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fractions hplc analysis/product/Waters Corporation
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    fractions hplc analysis - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    90
    Waters Corporation q tof hplc produced fractions
    High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus <t>UPE</t> extract and <t>HPLC</t> analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.
    Q Tof Hplc Produced Fractions, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q tof hplc produced fractions/product/Waters Corporation
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q tof hplc produced fractions - by Bioz Stars, 2020-08
    90/100 stars
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    90
    Waters Corporation e2695 separations module system
    High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus <t>UPE</t> extract and <t>HPLC</t> analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.
    E2695 Separations Module System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2695 separations module system/product/Waters Corporation
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    e2695 separations module system - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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    High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus UPE extract and HPLC analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.

    Journal: Molecules

    Article Title: Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction

    doi: 10.3390/molecules23123353

    Figure Lengend Snippet: High-speed counter-current chromatography (HSCCC) chromatogram for the separation of Ligustri Lucidi Fructus UPE extract and HPLC analysis of the peak fractions. Experimental conditions: solvent system, ethyl acetate: n -butanol:water (2:1:3, v / v ); the upper organic phase as the stationary phase and the lower aqueous phase as the mobile phase; revolution speed, 800 rpm; flow rate, 0–100 min, 2.0 mL·min −1 ; 100–170 min, 1.0 mL·min −1 ; 170–300 min, 2.0 mL·min −1 ; sample size, 200 mg; UV detection wavelength, 254 nm; retention of stationary phase, 65.7%.

    Article Snippet: HPLC Analysis and Identification of the Fractions HPLC analysis of the crude sample by UPE and fractions obtained from the HSCCC separation were performed on a Waters e2695 apparatus.

    Techniques: High Speed Counter-current Chromatography, High Performance Liquid Chromatography, Flow Cytometry

    HPLC chromatograms of the extraction samples by ultrasonic ( A ), heat reflux extraction ( B ), and UPE ( C ) from Ligustri Lucidi Fructus. HPLC conditions: column, Purospher STAR RP18 Endiapped (250 mm × 4.6 mm i.d., 5 μm); mobile phase, acetonitrile ( A )-water ( B ), 0–2 min, 5–5%A; 2–40 min, 5–30%A; 40–45 min, 30–30%A; 45–53 min, 30–50%A; column temperature, 25 °C; flow rate, 1.0 mL·min −1 ; UV detection wavelength, 282 nm; injection volume, 10 μL; peak 1, salidroside; peak 2, echinacoside; peak 3, acteoside; peak 4, specnuezhenide; peak 5, isonuezhenide; and peak 6, nuezhenoside G13.

    Journal: Molecules

    Article Title: Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction

    doi: 10.3390/molecules23123353

    Figure Lengend Snippet: HPLC chromatograms of the extraction samples by ultrasonic ( A ), heat reflux extraction ( B ), and UPE ( C ) from Ligustri Lucidi Fructus. HPLC conditions: column, Purospher STAR RP18 Endiapped (250 mm × 4.6 mm i.d., 5 μm); mobile phase, acetonitrile ( A )-water ( B ), 0–2 min, 5–5%A; 2–40 min, 5–30%A; 40–45 min, 30–30%A; 45–53 min, 30–50%A; column temperature, 25 °C; flow rate, 1.0 mL·min −1 ; UV detection wavelength, 282 nm; injection volume, 10 μL; peak 1, salidroside; peak 2, echinacoside; peak 3, acteoside; peak 4, specnuezhenide; peak 5, isonuezhenide; and peak 6, nuezhenoside G13.

    Article Snippet: HPLC Analysis and Identification of the Fractions HPLC analysis of the crude sample by UPE and fractions obtained from the HSCCC separation were performed on a Waters e2695 apparatus.

    Techniques: High Performance Liquid Chromatography, Reflux, Flow Cytometry, Injection

    Reverse-phase HPLC trace showing the separation of the subunits of complex I from P. pastoris . Proteins were eluted from a reverse-phase HPLC column, at 50 μl min −1 , in a 100-min linear gradient of 0-70% propan-2-ol and 15-20% trifluoroethanol, in 1% hexafluoroisopropanol and 50 m m ammonium formate (pH 3.1). The eluant was analyzed by an online ESI mass spectrometer (see Experimental Methods), so the elution trace is described by the total ion current. The labels denote detected molecular masses that could be assigned to subunits of P. pastoris complex I; the numbers refer to the subunits as listed in Table II .

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: The Subunit Composition of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) From Pichia pastoris

    doi: 10.1074/mcp.M110.001255

    Figure Lengend Snippet: Reverse-phase HPLC trace showing the separation of the subunits of complex I from P. pastoris . Proteins were eluted from a reverse-phase HPLC column, at 50 μl min −1 , in a 100-min linear gradient of 0-70% propan-2-ol and 15-20% trifluoroethanol, in 1% hexafluoroisopropanol and 50 m m ammonium formate (pH 3.1). The eluant was analyzed by an online ESI mass spectrometer (see Experimental Methods), so the elution trace is described by the total ion current. The labels denote detected molecular masses that could be assigned to subunits of P. pastoris complex I; the numbers refer to the subunits as listed in Table II .

    Article Snippet: Analysis of HPLC Fractions by ESI-MS Fractions from reverse-phase HPLC were analyzed by ESI-MS in positive ion mode, either online with a Quattro Ultima triple quadrupole mass spectrometer (Waters-Micromass, Waters Corp., Milford, MA) scanning 700–2100 m/z every 5 s, or offline using a Q-TOF1 mass spectrometer (Waters-Micromass).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry