atto425 protein labeling kit  (Jena Bioscience)


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    Jena Bioscience atto425 protein labeling kit
    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an <t>Atto425.</t> TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Atto425 Protein Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atto425 protein labeling kit/product/Jena Bioscience
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    atto425 protein labeling kit - by Bioz Stars, 2022-12
    88/100 stars

    Images

    1) Product Images from "Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB"

    Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2017.00067

    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Figure Legend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Techniques Used: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test

    2) Product Images from "Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB"

    Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2017.00067

    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Figure Legend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Techniques Used: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test

    3) Product Images from "Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB"

    Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2017.00067

    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Figure Legend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Techniques Used: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test

    4) Product Images from "Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB"

    Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2017.00067

    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Figure Legend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Techniques Used: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test

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  • 88
    Jena Bioscience atto425 protein labeling kit
    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an <t>Atto425.</t> TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Atto425 Protein Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atto425 protein labeling kit/product/Jena Bioscience
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atto425 protein labeling kit - by Bioz Stars, 2022-12
    88/100 stars
      Buy from Supplier

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    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

    doi: 10.3389/fcimb.2017.00067

    Figure Lengend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Article Snippet: To measure the amount of internalized TcdA, the primary amino groups of full-length TcdA were labeled using the Atto425 Protein Labeling Kit (Jena Bioscience GmbH) according to the manufacturer's protocol.

    Techniques: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test