Chromatin Immunoprecipitation:Article Title:
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Distinct radial glia subtypes regulate midbrain dopaminergic neuron development.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used TH (1:1000, Pel-Freez, P40101), Ki67 (1:500, Cell Signaling Technology, 9449), NR4A2/Nurr1 (1:500, Santa Cruz, sc990), βIII-tubulin (1:500, Promega, G7121), LMX1 (1:1000, Millipore, AB10533), FOXA2 (1:1000, R&D Systems, AF2400), BMAL1 (1:1000,Abcam, ab93806), yH2AX (1:500, Millipore, 05-636), and β-ACTIN (1:500, Abcam, ab6276). .. Secondary antibodies were AlexaFluor from Invitrogen.
Article Title: Phase I/II trial of iPS-cell-derived dopaminergic cells for Parkinson's disease.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Primary antibodies used are as follows: FOXA2 (goat, R&D systems, AF2400, 1:500), NURR1 (mouse, Perseus Proteomics, PPN1404-00, 1:300), HNA (mouse, Millipore, MAB1281, 1:500, KI67 (rabbit, Abcam, Ab16667, 1:1,000), 5-HT (rat, Millipore MAB352, 1:100), and TH (rabbit, Millipore, AB152, 1:400). .. Secondary antibodies are as follows: Alexa Fluor 488 anti-Mouse IgG, (donkey, ThermoFisher, A21202, 1:400), Alexa Fluor 594 anti-Goat IgG (donkey, ThermoFisher, A11058, 1:2,000), Alexa Fluor 594 anti-Rabbit IgG (donkey, ThermoFisher, A21207, 1:400), and Alexa Fluor 594 anti-Rat IgG (donkey, ThermoFisher, A21209, 1:400).
Flow Cytometry:Article Title:
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Distinct radial glia subtypes regulate midbrain dopaminergic neuron development.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used TH (1:1000, Pel-Freez, P40101), Ki67 (1:500, Cell Signaling Technology, 9449), NR4A2/Nurr1 (1:500, Santa Cruz, sc990), βIII-tubulin (1:500, Promega, G7121), LMX1 (1:1000, Millipore, AB10533), FOXA2 (1:1000, R&D Systems, AF2400), BMAL1 (1:1000,Abcam, ab93806), yH2AX (1:500, Millipore, 05-636), and β-ACTIN (1:500, Abcam, ab6276). .. Secondary antibodies were AlexaFluor from Invitrogen.
Article Title: Phase I/II trial of iPS-cell-derived dopaminergic cells for Parkinson's disease.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Primary antibodies used are as follows: FOXA2 (goat, R&D systems, AF2400, 1:500), NURR1 (mouse, Perseus Proteomics, PPN1404-00, 1:300), HNA (mouse, Millipore, MAB1281, 1:500, KI67 (rabbit, Abcam, Ab16667, 1:1,000), 5-HT (rat, Millipore MAB352, 1:100), and TH (rabbit, Millipore, AB152, 1:400). .. Secondary antibodies are as follows: Alexa Fluor 488 anti-Mouse IgG, (donkey, ThermoFisher, A21202, 1:400), Alexa Fluor 594 anti-Goat IgG (donkey, ThermoFisher, A11058, 1:2,000), Alexa Fluor 594 anti-Rabbit IgG (donkey, ThermoFisher, A21207, 1:400), and Alexa Fluor 594 anti-Rat IgG (donkey, ThermoFisher, A21209, 1:400).
Magnetic Resonance Imaging:Article Title:
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used AP2-alpha (Santa Cruz Biotechnology; sc-12726; clone 3B5; 1:200); AP2-gamma (R&D Systems; AF5059; 1:500); AP2-gamma (Santa Cruz Biotechnology, sc-12762; clone 6E4/4; 1:200); Brachyury (R&D Systems; AF2085; 1:500); CDX2 (BioGenex; MU392-UC; clone 4 nature portfolio | reporting sum m ary M arch 2021 CDX2-88; 1:200); CER1 (R&D Systems; AF1075; 1:500); Cytokeratin 7 (Aligent; M7018; clone OV-TL 12/30; 1:100); E-Cadherin (BD Biosciences; 610182; clone 36; 1:200); EOMES (Abcam; ab23345; 1:200); FOXA2 (R&D Systems; AF2400; 1:200); GATA2 (Novus Biologics; NBMP1-82581; 1:200); GATA3 (Abcam; ab199428; clone EPR16651; 1:500); GATA4 (Santa Cruz Biotechnology; sc-25310; clone G-4; 1:200); GATA4 (ThermoFisher Scientific; 14-9980-82; clone eBioEvan; 1:500); GATA6 (R&D Systems; AF1700; 1:500); GATA6 (Cell Signaling Technology; 5851; clone D61E4; 1:2000); GFP (Abcam; ab13970; 1:1000); GFP (Nacalai USA; GF090R; clone GF090R; 1:1000); HAND1 (DSHB; PCRP-HAND1-2A9; clone 2A9 1:200); HNF4-alpha (Abcam; ab201460; clone EPR16885-99; 1:2000); ISL1 (DSHB; PCRP-ISL1-1A9; clone 1A9; 1:100); Laminin (Sigma Aldrich; L9393; 1:200); N-Cadherin (Abcam; ab98952; clone 5D5; 1:200); NANOG (Cell Signaling Technology; 4903; clone D73G4; 1:200); OCT3/4 (Santa Cruz Biotechnology; sc-5279; clone C-10; 1:100); OTX2 (R&D Systems; AF1979; 1:1000); phospho-SMAD1/5 (Cell Signaling Technology; 9516S; clone 41D10; 1:200); Smad2/3 (Cell Signaling Technology; 8685S; clone D7G7; 1:200); SOX17 (R&D Systems; AF1924; 1:500); SOX2 (ThermoFisher Scientific; 14-9811-82; clone Btjce; 1:500); TBX20 (R&D Systems; MAB8124; clone 668710; 1:100); VTCN1 (Abcam; ab209242; clone EPR20236; 1:200). .. AlexaFluor-405 Donkey Anti-Mouse (ThermoFisher Scientific; A48257; 1:500); AlexaFluor-488 Donkey Anti-Rat (ThermoFisher Scientific; A-21208; 1:500); AlexFluor-488 Donkey Anti-Mouse (ThermoFisher Scientific; A-21202; 1:500); AlexaFluor-488 Donkey Anti-Goat (ThermoFisher Scientific; A-11055; 1:500); AlexaFluor-568 Donkey Anti-Rabbit (ThermoFisher Scientific; A10042; 1:500); AlexaFluor-568 Donkey Anti-Rat (ThermoFisher Scientific; A78946; 1:500); AlexaFluor-568 Donkey Anti-Mouse (ThermoFisher Scientific; A10037; 1:500); AlexaFluor-647 Donkey Anti-Rat (ThermoFisher Scientific; A78947; 1:500); AlexaFluor-647 Donkey AntiGoat (ThermoFisher Scientific; A-21447; 1:500); AlexaFluor-647 Donkey Anti-Rabbit (Thermofisher Scientific; A-31573; 1:500); AlexaFluor-647 Donkey Anti-Mouse (ThermoFisher Scientific; A32787; 1:500).
Article Title: Distinct radial glia subtypes regulate midbrain dopaminergic neuron development.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used TH (1:1000, Pel-Freez, P40101), Ki67 (1:500, Cell Signaling Technology, 9449), NR4A2/Nurr1 (1:500, Santa Cruz, sc990), βIII-tubulin (1:500, Promega, G7121), LMX1 (1:1000, Millipore, AB10533), FOXA2 (1:1000, R&D Systems, AF2400), BMAL1 (1:1000,Abcam, ab93806), yH2AX (1:500, Millipore, 05-636), and β-ACTIN (1:500, Abcam, ab6276). .. Secondary antibodies were AlexaFluor from Invitrogen.
Article Title: Phase I/II trial of iPS-cell-derived dopaminergic cells for Parkinson's disease.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Primary antibodies used are as follows: FOXA2 (goat, R&D systems, AF2400, 1:500), NURR1 (mouse, Perseus Proteomics, PPN1404-00, 1:300), HNA (mouse, Millipore, MAB1281, 1:500, KI67 (rabbit, Abcam, Ab16667, 1:1,000), 5-HT (rat, Millipore MAB352, 1:100), and TH (rabbit, Millipore, AB152, 1:400). .. Secondary antibodies are as follows: Alexa Fluor 488 anti-Mouse IgG, (donkey, ThermoFisher, A21202, 1:400), Alexa Fluor 594 anti-Goat IgG (donkey, ThermoFisher, A11058, 1:2,000), Alexa Fluor 594 anti-Rabbit IgG (donkey, ThermoFisher, A21207, 1:400), and Alexa Fluor 594 anti-Rat IgG (donkey, ThermoFisher, A21209, 1:400).
Immunohistochemistry:Article Title: Phase I/II trial of iPS-cell-derived dopaminergic cells for Parkinson's disease.
Article Snippet: .. Primary antibodies used for immunohistochemistry are as follows: FOXA2 (goat, R&D systems, AF2400, 1:500), NURR1 (mouse, Perseus Proteomics, PP-N1404-00, 1:300), human nucleic antigen (HNA) (mouse, Millipore, MAB1281, 1:500, Ki-67 (rabbit, Abcam, Ab16667, 1:1,000), 5-HT (rat, Millipore MAB352, 1:100) and TH (rabbit, Millipore, AB152, 1:400). .. Secondary antibodies are as follows: Alexa Fluor 488 anti-Mouse IgG, (donkey, Thermo Fisher Scientific, A21202, 1:400), Alexa Fluor 594 anti-Goat IgG (donkey, Thermo Fisher Scientific, A11058, 1:2,000), Alexa Fluor 594 anti-rabbit IgG (donkey, Thermo Fisher Scientific, A21207, 1:400) and Alexa Fluor 594 anti-rat IgG (donkey, Thermo Fisher Scientific, A21209, 1:400).
Article Title: Phase I/II trial of iPS-cell-derived dopaminergic cells for Parkinson’s disease
Article Snippet: .. Primary antibodies used for immunohistochemistry are as follows: FOXA2 (goat, R&D systems, AF2400, 1:500), NURR1 (mouse, Perseus Proteomics, PP-N1404-00, 1:300), human nucleic antigen (HNA) (mouse, Millipore, MAB1281, 1:500, Ki-67 (rabbit, Abcam, Ab16667, 1:1,000), 5-HT (rat, Millipore MAB352, 1:100) and TH (rabbit, Millipore, AB152, 1:400). .. Secondary antibodies are as follows: Alexa Fluor 488 anti-Mouse IgG, (donkey, Thermo Fisher Scientific, A21202, 1:400), Alexa Fluor 594 anti-Goat IgG (donkey, Thermo Fisher Scientific, A11058, 1:2,000), Alexa Fluor 594 anti-rabbit IgG (donkey, Thermo Fisher Scientific, A21207, 1:400) and Alexa Fluor 594 anti-rat IgG (donkey, Thermo Fisher Scientific, A21209, 1:400).
Incubation:Article Title: TFEB and TFE3 have cell-type specific expression in the brain and divergent roles in neurons
Article Snippet: .. Primary antibodies (TFEB (Cell Signalling) – 1:500, TFEB (Bethyl) – 1:1000, TFE3 (abcam) – 1:1000, TH (abcam, ab76442) – 1:1000, TH (Millipore, AB152) – 1:500, MAP2 (Millipore, ab92434) – 1:2000, FOXA2 (R&D systems, AF2400) – 1:250, Iba1 (abcam, ab5076) – 1:1000, LMX1A (abcam, ab139726) – 1:1000, TUJ1 (Biolegend, 801202) – 1:500, LAMP1 (Santa Cruz) – 1:500, TOM20 (Santa Cruz) – 1:1000) were incubated overnight at 4°C. .. Cells were then washed twice PBS and secondary antibodies (1:1000 dilution); α-Ms AF555 (ThermoFisher, A31570), α-Chk AF488 (ThermoFisher, A78948), α-Rb AF647 (ThermoFisher, A32795), α-Gt AF488 (ThermoFisher, A11055), α-Rb AF488 (ThermoFisher, A21206), α-Rb AF555 (ThermoFisher, A31572), α-Chk AF555 (ThermoFisher, A78949) were incubated at room temperature for 2 hours.
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