focused ultrasonication  (Covaris)


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    Covaris focused ultrasonication
    Focused Ultrasonication, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/focused ultrasonication/product/Covaris
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    focused ultrasonication - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Integrated genomic characterization of oesophageal carcinoma
    Article Snippet: .. Low-pass whole-genome sequencing for rearrangement identification Genomic DNA (500–700 ng per sample) was sheared into 250-bp fragments using a Covaris E220 ultrasonicator, then converted to a paired-end Illumina library using KAPA Bio kits with Caliper (PerkinElmer) robotic NGS Suite (Partek Genomics) according to manufacturers’ protocols. ..

    Sonication:

    Article Title: Assessing the Impact of Cyclosporin A on Lentiviral Transduction and Preservation of Human Hematopoietic Stem Cells in Clinically Relevant Ex Vivo Gene Therapy Settings
    Article Snippet: .. Briefly, the genomic DNA from transduced cells was fragmented by adaptive focused acoustic sonication using a Covaris E220 Ultrasonicator (Covaris, Inc., Woburn, MA). .. The fragmented DNA was then subjected to end repair and 3′ adenylation, ligated to the DNA blunted-end linker cassette using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® following the manufacturer's instructions (New England Biolabs, Ipswich, MA).

    Article Title: An RNA-Centric Dissection of Host Complexes Controlling Flavivirus Infection
    Article Snippet: .. Lysates were sonicated using a focused-ultrasonicator (Covaris, E220) until the average RNA length was 500 nucleotides as determined by agarose gel analysis and stored at −80°C. .. Stored lysates were thawed on ice, and for every 1 mL of sonicated lysate 2 mL of ChIRP hybridization buffer (750 mM NaCl, 1% SDS, 50 mM Tris-HCl pH 7.0, 1 mM EDTA, 15% formamide; made fresh) and precleared by adding 30 μL washed MyOne C1 beads per mL of lysate at 37°C for 30 minutes on rotation.

    Article Title: Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity
    Article Snippet: .. For Med1 and Nanog IP, cells were resuspended in 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 2 mM EDTA, 0.1% SDS and 1% Triton X-100 and sonicated for approximately 8 min using a Covaris E220 ultrasonicator. .. The resulting whole cell extract was centrifuged and the supernatant was incubated for 1 h at 4 °C with 50 μL of protein G magnetic beads that had been pre-incubated with 5 μg of control rabbit IgG.

    Next-Generation Sequencing:

    Article Title: Integrated genomic characterization of oesophageal carcinoma
    Article Snippet: .. Low-pass whole-genome sequencing for rearrangement identification Genomic DNA (500–700 ng per sample) was sheared into 250-bp fragments using a Covaris E220 ultrasonicator, then converted to a paired-end Illumina library using KAPA Bio kits with Caliper (PerkinElmer) robotic NGS Suite (Partek Genomics) according to manufacturers’ protocols. ..

    Agarose Gel Electrophoresis:

    Article Title: An RNA-Centric Dissection of Host Complexes Controlling Flavivirus Infection
    Article Snippet: .. Lysates were sonicated using a focused-ultrasonicator (Covaris, E220) until the average RNA length was 500 nucleotides as determined by agarose gel analysis and stored at −80°C. .. Stored lysates were thawed on ice, and for every 1 mL of sonicated lysate 2 mL of ChIRP hybridization buffer (750 mM NaCl, 1% SDS, 50 mM Tris-HCl pH 7.0, 1 mM EDTA, 15% formamide; made fresh) and precleared by adding 30 μL washed MyOne C1 beads per mL of lysate at 37°C for 30 minutes on rotation.

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    Covaris focused ultrasonicator
    Identification of active deubiquitinases in bovine lung tissue (A) . Lung tissue from control and BRD-affected cows was subjected to lysis by <t>focused-ultrasonicator,</t> followed by reaction with HA-Ub-VS probe to label active deubiquitinases. The anti-HA western blotting was done to visualize the deubiquitinases, anti-beta actin blot was done as a loading control. Anti-USP15 western blot was also performed. (B) . Bovine deubiquitinases from (A) were subjected to immunoprecipitation and proteomic analysis. Relative fold change for one significantly down-regulated protein (p
    Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/focused ultrasonicator/product/Covaris
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    focused ultrasonicator - by Bioz Stars, 2020-05
    99/100 stars
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    Identification of active deubiquitinases in bovine lung tissue (A) . Lung tissue from control and BRD-affected cows was subjected to lysis by focused-ultrasonicator, followed by reaction with HA-Ub-VS probe to label active deubiquitinases. The anti-HA western blotting was done to visualize the deubiquitinases, anti-beta actin blot was done as a loading control. Anti-USP15 western blot was also performed. (B) . Bovine deubiquitinases from (A) were subjected to immunoprecipitation and proteomic analysis. Relative fold change for one significantly down-regulated protein (p

    Journal: Analytical biochemistry

    Article Title: Use of focused ultrasonication in activity-based profiling of deubiquitinating enzymes in tissue

    doi: 10.1016/j.ab.2016.09.016

    Figure Lengend Snippet: Identification of active deubiquitinases in bovine lung tissue (A) . Lung tissue from control and BRD-affected cows was subjected to lysis by focused-ultrasonicator, followed by reaction with HA-Ub-VS probe to label active deubiquitinases. The anti-HA western blotting was done to visualize the deubiquitinases, anti-beta actin blot was done as a loading control. Anti-USP15 western blot was also performed. (B) . Bovine deubiquitinases from (A) were subjected to immunoprecipitation and proteomic analysis. Relative fold change for one significantly down-regulated protein (p

    Article Snippet: The use of focused-ultrasonicator for proteomics has not yet been established.

    Techniques: Lysis, Western Blot, Immunoprecipitation