fmoc cha oh  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Name:
    Fmoc Cha OH
    Description:

    Catalog Number:
    47314
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore fmoc cha oh
    Fmoc Cha OH

    https://www.bioz.com/result/fmoc cha oh/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fmoc cha oh - by Bioz Stars, 2020-09
    85/100 stars

    Images

    Related Articles

    other:

    Article Title: Deletion of Ac-NMePhe1 from [NMePhe1]arodyn under Acidic Conditions: 2. Effects of Substitutions on Pharmacological Activity
    Article Snippet: Fmoc-Cha-OH was purchased from Calbiochem-Novabiochem (San Diego, CA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore pp2a phosphatase assay
    <t>PP2A</t> is important for B cell activation and Ig production in vitro . ( A ) Western blot analysis of PP2Aa and PP2Ac subunit expression in isolated B cells from the indicated mice. Quantification of PP2Ac expression in isolated B cells from the indicated mice. ( B ) ELISA analysis of the indicated serum Ig levels from the indicated mice (12–24 weeks old) ( n = 3 mice per group for 2 independent experiments). ( C and D ) Splenic B cells were isolated from the indicated mice and were stimulated with either CpG or anti-CD40 in the presence of IL-4 for 72 hours. ( C ) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells with CpG and IL-4 stimulation. Middle: Dot plots represent the percentages of IgG + CD138 + plasma cells in total cultured B cells in the presence of CpG plus IL-4. Right: qPCR analysis on the expression of the indicated gene expression by the indicated B cells ( n = 3 per group for 2 independent experiments). ( D ) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells after anti-CD40 and IL-4 stimulation. Middle: Dot plots indicate the percentages of IgG + CD138 + plasma cells in total B cells cultured with anti-CD40 and IL-4. Right: qPCR analysis of indicated genes in the indicated B cells ( n = 3 per group for 2 independent experiments). Paired t test, mean ± SEM.
    Pp2a Phosphatase Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp2a phosphatase assay/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pp2a phosphatase assay - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    PP2A is important for B cell activation and Ig production in vitro . ( A ) Western blot analysis of PP2Aa and PP2Ac subunit expression in isolated B cells from the indicated mice. Quantification of PP2Ac expression in isolated B cells from the indicated mice. ( B ) ELISA analysis of the indicated serum Ig levels from the indicated mice (12–24 weeks old) ( n = 3 mice per group for 2 independent experiments). ( C and D ) Splenic B cells were isolated from the indicated mice and were stimulated with either CpG or anti-CD40 in the presence of IL-4 for 72 hours. ( C ) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells with CpG and IL-4 stimulation. Middle: Dot plots represent the percentages of IgG + CD138 + plasma cells in total cultured B cells in the presence of CpG plus IL-4. Right: qPCR analysis on the expression of the indicated gene expression by the indicated B cells ( n = 3 per group for 2 independent experiments). ( D ) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells after anti-CD40 and IL-4 stimulation. Middle: Dot plots indicate the percentages of IgG + CD138 + plasma cells in total B cells cultured with anti-CD40 and IL-4. Right: qPCR analysis of indicated genes in the indicated B cells ( n = 3 per group for 2 independent experiments). Paired t test, mean ± SEM.

    Journal: JCI Insight

    Article Title: Serine/threonine phosphatase PP2A is essential for optimal B cell function

    doi: 10.1172/jci.insight.130655

    Figure Lengend Snippet: PP2A is important for B cell activation and Ig production in vitro . ( A ) Western blot analysis of PP2Aa and PP2Ac subunit expression in isolated B cells from the indicated mice. Quantification of PP2Ac expression in isolated B cells from the indicated mice. ( B ) ELISA analysis of the indicated serum Ig levels from the indicated mice (12–24 weeks old) ( n = 3 mice per group for 2 independent experiments). ( C and D ) Splenic B cells were isolated from the indicated mice and were stimulated with either CpG or anti-CD40 in the presence of IL-4 for 72 hours. ( C ) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells with CpG and IL-4 stimulation. Middle: Dot plots represent the percentages of IgG + CD138 + plasma cells in total cultured B cells in the presence of CpG plus IL-4. Right: qPCR analysis on the expression of the indicated gene expression by the indicated B cells ( n = 3 per group for 2 independent experiments). ( D ) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells after anti-CD40 and IL-4 stimulation. Middle: Dot plots indicate the percentages of IgG + CD138 + plasma cells in total B cells cultured with anti-CD40 and IL-4. Right: qPCR analysis of indicated genes in the indicated B cells ( n = 3 per group for 2 independent experiments). Paired t test, mean ± SEM.

    Article Snippet: PP2A phosphatase assay.B cells were isolated from mouse spleens, and PP2A phosphatase assay was performed as manufactory’s instructions (MilliporeSigma, catalog 17-313).

    Techniques: Activation Assay, In Vitro, Western Blot, Expressing, Isolation, Mouse Assay, Enzyme-linked Immunosorbent Assay, Produced, Cell Culture, Real-time Polymerase Chain Reaction

    PP2A in B cells inhibits mitochondrial respiration by suppressing the expression of purine nucleoside phosphorylase (PNP). ( A ) Dot plots show mitochondrial respiration in Ppp2r1a- deficient mouse splenic B cells compared with WT mouse splenic B cells (maximal oxygen consumption rate [OCR]and space capacity OCR) ( n = 4 independent experiments). ( B ) Joint analysis of RNA sequencing and metabolomics in B cells from flox/flox mice compared with control mice ( n = 3 mice per group). ( C ) Increased PNP mRNA expression in B cells from flox/flox mice compared with control mice ( n = 6 mice per group for qPCR experiment). ( D ) Western blot analysis on PNP expression in mouse splenic B cells with PP2Aa deficiency compared with WT controls. Quantification of Western blots. ( E ) Human primary B cells were enriched from peripheral blood mononuclear cells (PBMC). Left: Representation of oxygen consumption rate (OCR) of human primary B cells exposed to the indicated treatments (9-Deazaguanine was applied as PNP inhibitor). Right: Dot plots indicate mitochondrial respiration in human primary B cells with PP2Aa deficiency compared with controls exposed to the indicated treatments. Paired t test, mean ± SEM.

    Journal: JCI Insight

    Article Title: Serine/threonine phosphatase PP2A is essential for optimal B cell function

    doi: 10.1172/jci.insight.130655

    Figure Lengend Snippet: PP2A in B cells inhibits mitochondrial respiration by suppressing the expression of purine nucleoside phosphorylase (PNP). ( A ) Dot plots show mitochondrial respiration in Ppp2r1a- deficient mouse splenic B cells compared with WT mouse splenic B cells (maximal oxygen consumption rate [OCR]and space capacity OCR) ( n = 4 independent experiments). ( B ) Joint analysis of RNA sequencing and metabolomics in B cells from flox/flox mice compared with control mice ( n = 3 mice per group). ( C ) Increased PNP mRNA expression in B cells from flox/flox mice compared with control mice ( n = 6 mice per group for qPCR experiment). ( D ) Western blot analysis on PNP expression in mouse splenic B cells with PP2Aa deficiency compared with WT controls. Quantification of Western blots. ( E ) Human primary B cells were enriched from peripheral blood mononuclear cells (PBMC). Left: Representation of oxygen consumption rate (OCR) of human primary B cells exposed to the indicated treatments (9-Deazaguanine was applied as PNP inhibitor). Right: Dot plots indicate mitochondrial respiration in human primary B cells with PP2Aa deficiency compared with controls exposed to the indicated treatments. Paired t test, mean ± SEM.

    Article Snippet: PP2A phosphatase assay.B cells were isolated from mouse spleens, and PP2A phosphatase assay was performed as manufactory’s instructions (MilliporeSigma, catalog 17-313).

    Techniques: Expressing, RNA Sequencing Assay, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot

    Increased PP2A expression and enhanced function in activated B cells or B cells from lupus-prone mice and SLE patients. ( A ) Western blot analysis on the expression of both scaffold (PP2A A ) and catalytic (PP2A C ) subunits in splenic B cells isolated from the indicated mice. Quantification of Western blots ( n = 3 per experiment, a representative experiment and pooled densitometry of the 3 experiments are shown). ( B ) Germinal center B cells (GC, CD19 + FAS + GL7 + ), plasma cells (PC, CD19 + IgG + CD138 hi ), marginal zone B cells (MZ, CD19 + CD21 + CD23 lo ), and follicular B cells (FO, CD19 + CD21 lo CD23 hi ) were FACS sorted for qPCR. Dot plots show the expression of PP2A A , and elevated expression was observed in GC and PC compared with MZ and FO. ( C ) Flow cytometry analysis of the expression of PP2Ac (indicated by mean florescence intensity, MFI) in total B cells from patients with SLE and matched healthy controls. ( D ) Dot plots show the increased MFI of PP2Ac in DN B cells (CD19 + IgD – CD27 – ), memory B cells (CD19 + IgD – CD27 + ), and plasma B cells (CD19 + IgG + CD138 + ) compared with naive B cells (CD19 + IgD + CD27 – ). ( E–H ) CD19 + human B cells were enriched by magnetic beads and cultured with the indicated stimuli for the indicated time. ( E ) Dot plots show the expression of PP2Aa in B cells stimulated with either CpG or anti-CD40 compared with unstimulated B cells 3 hours after stimulation. ( F ) Dot plots show the expression of PP2Aa in B cells stimulated with either CpG or anti-CD40 compared with unstimulated B cells 3 hours after stimulation. ( G ) Western blot analysis of the expression of PP2Aa and PP2Ac in human B cells stimulated with either CpG or anti-CD40 for 48 hours. ( H ) Dot plots show the quantification of PP2Aa and expression PP2Ac in human B cells stimulated with either CpG or anti-CD40 compared with control unstimulated B cells 3 hours after stimulation. ( I and J ) PP2A phosphatase activity was quantified using a kit from R D. The activity of PP2A is presented as the rate of phosphate release (pmol × 10 2 ). ( I ) Bar graph shows the PP2A phosphatase activity in ex vivo splenic B cells from lupus-prone Mrl.lpr mice compared with matching control Mrl.mpj mice (data pooled from 3 independent experiments, paired t test, mean ± SEM). ( J ) Splenic B cells were enriched from C57BL/6J WT mice by MACS and stimulated with the indicated reagents for several time periods; kinetic curves show the PP2A phosphatase activity ( n = 3 for 3 independent experiments, 2-way ANOVA analysis, mean ± SEM).

    Journal: JCI Insight

    Article Title: Serine/threonine phosphatase PP2A is essential for optimal B cell function

    doi: 10.1172/jci.insight.130655

    Figure Lengend Snippet: Increased PP2A expression and enhanced function in activated B cells or B cells from lupus-prone mice and SLE patients. ( A ) Western blot analysis on the expression of both scaffold (PP2A A ) and catalytic (PP2A C ) subunits in splenic B cells isolated from the indicated mice. Quantification of Western blots ( n = 3 per experiment, a representative experiment and pooled densitometry of the 3 experiments are shown). ( B ) Germinal center B cells (GC, CD19 + FAS + GL7 + ), plasma cells (PC, CD19 + IgG + CD138 hi ), marginal zone B cells (MZ, CD19 + CD21 + CD23 lo ), and follicular B cells (FO, CD19 + CD21 lo CD23 hi ) were FACS sorted for qPCR. Dot plots show the expression of PP2A A , and elevated expression was observed in GC and PC compared with MZ and FO. ( C ) Flow cytometry analysis of the expression of PP2Ac (indicated by mean florescence intensity, MFI) in total B cells from patients with SLE and matched healthy controls. ( D ) Dot plots show the increased MFI of PP2Ac in DN B cells (CD19 + IgD – CD27 – ), memory B cells (CD19 + IgD – CD27 + ), and plasma B cells (CD19 + IgG + CD138 + ) compared with naive B cells (CD19 + IgD + CD27 – ). ( E–H ) CD19 + human B cells were enriched by magnetic beads and cultured with the indicated stimuli for the indicated time. ( E ) Dot plots show the expression of PP2Aa in B cells stimulated with either CpG or anti-CD40 compared with unstimulated B cells 3 hours after stimulation. ( F ) Dot plots show the expression of PP2Aa in B cells stimulated with either CpG or anti-CD40 compared with unstimulated B cells 3 hours after stimulation. ( G ) Western blot analysis of the expression of PP2Aa and PP2Ac in human B cells stimulated with either CpG or anti-CD40 for 48 hours. ( H ) Dot plots show the quantification of PP2Aa and expression PP2Ac in human B cells stimulated with either CpG or anti-CD40 compared with control unstimulated B cells 3 hours after stimulation. ( I and J ) PP2A phosphatase activity was quantified using a kit from R D. The activity of PP2A is presented as the rate of phosphate release (pmol × 10 2 ). ( I ) Bar graph shows the PP2A phosphatase activity in ex vivo splenic B cells from lupus-prone Mrl.lpr mice compared with matching control Mrl.mpj mice (data pooled from 3 independent experiments, paired t test, mean ± SEM). ( J ) Splenic B cells were enriched from C57BL/6J WT mice by MACS and stimulated with the indicated reagents for several time periods; kinetic curves show the PP2A phosphatase activity ( n = 3 for 3 independent experiments, 2-way ANOVA analysis, mean ± SEM).

    Article Snippet: PP2A phosphatase assay.B cells were isolated from mouse spleens, and PP2A phosphatase assay was performed as manufactory’s instructions (MilliporeSigma, catalog 17-313).

    Techniques: Expressing, Mouse Assay, Western Blot, Isolation, FACS, Real-time Polymerase Chain Reaction, Flow Cytometry, Magnetic Beads, Cell Culture, Activity Assay, Ex Vivo, Magnetic Cell Separation

    PP2A is critical for B cell activation, differentiation, and Ig production in vivo. ( A ) Dot plots show the percentages of spontaneous germinal center B cells (GC-CD19 + FAS + PNA + ) and T follicular helper cells (Tfh-CD4 + CXCR5 hi PD1 hi ) in spleens of the indicated 24-week-old mice ( n = 6 mice per group for 2 independent experiments). ( B and C ) Mice were immunized i.p. with 0.2 mL/mouse SRBC. ( B ) IHC staining of frozen spleen sections of the indicated mice ( n = 3 mice per group in 2 independent experiments). Bar graphs show the quantification (percentage) of follicles with germinal centers in total splenic follicles from the indicated mice ( n = 3 mice per group in 2 independent experiments). ( C ) Flow cytometry analysis (%) of germinal center B cells (GC-CD19 + FAS + PNA + ), T follicular helper cells (Tfh-CD4 + CXCR5 hi PD1 hi ), and IgG plasma cells (PC-CD19 + IgG + CD138 + ) in the spleens of the indicated mice after SRBC immunization. ( D and E ) Indicated mice were i.p. immunized with 100 μg/mouse NP-Ficoll for 5 days or 100 μg/mouse NP-CGG in 5% alum for 14 days. ( D ) ELISA analysis for high affinity (NP-7) or low affinity (NP-41) for NP antigen-specific IgM in the serum of 12-week-old flox/flox mice compared with control mice 5 days after immunization with T-independent antigen NP-Ficoll ( n = 3 per group in 2 independent experiments). ( E ) ELISA analysis of high affinity (NP-7) or all affinity (NP-41) NP antigen-specific IgG in the serum of 12-week-old flox/flox mice compared with control mice 14 days after immunization with T-dependent antigen NP-CGG ( n = 3 per group in 2 independent experiments). Paired t test, ** P

    Journal: JCI Insight

    Article Title: Serine/threonine phosphatase PP2A is essential for optimal B cell function

    doi: 10.1172/jci.insight.130655

    Figure Lengend Snippet: PP2A is critical for B cell activation, differentiation, and Ig production in vivo. ( A ) Dot plots show the percentages of spontaneous germinal center B cells (GC-CD19 + FAS + PNA + ) and T follicular helper cells (Tfh-CD4 + CXCR5 hi PD1 hi ) in spleens of the indicated 24-week-old mice ( n = 6 mice per group for 2 independent experiments). ( B and C ) Mice were immunized i.p. with 0.2 mL/mouse SRBC. ( B ) IHC staining of frozen spleen sections of the indicated mice ( n = 3 mice per group in 2 independent experiments). Bar graphs show the quantification (percentage) of follicles with germinal centers in total splenic follicles from the indicated mice ( n = 3 mice per group in 2 independent experiments). ( C ) Flow cytometry analysis (%) of germinal center B cells (GC-CD19 + FAS + PNA + ), T follicular helper cells (Tfh-CD4 + CXCR5 hi PD1 hi ), and IgG plasma cells (PC-CD19 + IgG + CD138 + ) in the spleens of the indicated mice after SRBC immunization. ( D and E ) Indicated mice were i.p. immunized with 100 μg/mouse NP-Ficoll for 5 days or 100 μg/mouse NP-CGG in 5% alum for 14 days. ( D ) ELISA analysis for high affinity (NP-7) or low affinity (NP-41) for NP antigen-specific IgM in the serum of 12-week-old flox/flox mice compared with control mice 5 days after immunization with T-independent antigen NP-Ficoll ( n = 3 per group in 2 independent experiments). ( E ) ELISA analysis of high affinity (NP-7) or all affinity (NP-41) NP antigen-specific IgG in the serum of 12-week-old flox/flox mice compared with control mice 14 days after immunization with T-dependent antigen NP-CGG ( n = 3 per group in 2 independent experiments). Paired t test, ** P

    Article Snippet: PP2A phosphatase assay.B cells were isolated from mouse spleens, and PP2A phosphatase assay was performed as manufactory’s instructions (MilliporeSigma, catalog 17-313).

    Techniques: Activation Assay, In Vivo, Mouse Assay, Immunohistochemistry, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    PP2A expression in B cells for disease development in mice after injection of pristine. ( A and B ) Indicated mice were challenged i.p. with 0.5 mL pristine. ( A ) Blot graph shows ANA antibody levels in the serum of flox/flox and control mice 1 month after pristine challenge ( n = 6 mice per group). ( B ) Immunofluorescence image analysis to detect IgG deposition in the kidneys of flox/flox and control mice 6 months after pristine challenge ( n = 6 mice per group). ( C ) Western blot analysis on pSTAT3 and total STAT3 expression in B cells with PP2A deficiency. Quantification of Western blots. ( D–F ) Human B cells were enriched from peripheral blood mononuclear cells (PBMC) using MACS cell separation kits. ( D ) Western blot analysis to detect PP2Aa and PP2Ac in human B cells treated with control or PP2Aa siRNA. Quantification of Western blot densities. ( E ) Flow cytometry analysis on pSTAT3 induction during B cell activation in the absence of PP2Aa. ( F ) Immunofluorescence image analysis on pSTAT3 expression during B cell activation in the absence of PP2Aa. Original magnification ×63. Paired t test, * P

    Journal: JCI Insight

    Article Title: Serine/threonine phosphatase PP2A is essential for optimal B cell function

    doi: 10.1172/jci.insight.130655

    Figure Lengend Snippet: PP2A expression in B cells for disease development in mice after injection of pristine. ( A and B ) Indicated mice were challenged i.p. with 0.5 mL pristine. ( A ) Blot graph shows ANA antibody levels in the serum of flox/flox and control mice 1 month after pristine challenge ( n = 6 mice per group). ( B ) Immunofluorescence image analysis to detect IgG deposition in the kidneys of flox/flox and control mice 6 months after pristine challenge ( n = 6 mice per group). ( C ) Western blot analysis on pSTAT3 and total STAT3 expression in B cells with PP2A deficiency. Quantification of Western blots. ( D–F ) Human B cells were enriched from peripheral blood mononuclear cells (PBMC) using MACS cell separation kits. ( D ) Western blot analysis to detect PP2Aa and PP2Ac in human B cells treated with control or PP2Aa siRNA. Quantification of Western blot densities. ( E ) Flow cytometry analysis on pSTAT3 induction during B cell activation in the absence of PP2Aa. ( F ) Immunofluorescence image analysis on pSTAT3 expression during B cell activation in the absence of PP2Aa. Original magnification ×63. Paired t test, * P

    Article Snippet: PP2A phosphatase assay.B cells were isolated from mouse spleens, and PP2A phosphatase assay was performed as manufactory’s instructions (MilliporeSigma, catalog 17-313).

    Techniques: Expressing, Mouse Assay, Injection, Immunofluorescence, Western Blot, Magnetic Cell Separation, Flow Cytometry, Activation Assay