Structured Review

Cayman Chemical flurbiprofen
DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and <t>S-flurbiprofen,</t> gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism"

Article Title: Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism

Journal: bioRxiv

doi: 10.1101/2024.01.17.576032

DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .
Figure Legend Snippet: DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .

Techniques Used: Fluorescence, Titration, Binding Assay

Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. ( A and B ) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. ( C and D ) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F ) Molecular stick models showing the distinct positions of (R)-(E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.
Figure Legend Snippet: Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. ( A and B ) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. ( C and D ) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F ) Molecular stick models showing the distinct positions of (R)-(E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.

Techniques Used: Binding Assay


Structured Review

Cayman Chemical flurbiprofen
Arachidonic Acid as a Model Ligand for DAUDA Displacement Assays with hFABP1.
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism"

Article Title: Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism

Journal: bioRxiv

doi: 10.1101/2024.01.17.576032

Arachidonic Acid as a Model Ligand for DAUDA Displacement Assays with hFABP1.
Figure Legend Snippet: Arachidonic Acid as a Model Ligand for DAUDA Displacement Assays with hFABP1.

Techniques Used: Fluorescence

DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine Kd values summarized in Table 1.
Figure Legend Snippet: DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine Kd values summarized in Table 1.

Techniques Used: Fluorescence, Titration, Binding Assay

Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. (A and B) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. (C and D) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F) Molecular stick models showing the distinct positions of (R)- (E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.
Figure Legend Snippet: Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. (A and B) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. (C and D) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F) Molecular stick models showing the distinct positions of (R)- (E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.

Techniques Used: Binding Assay


Structured Review

Cayman Chemical flurbiprofen
Arachidonic Acid as a Model Ligand for DAUDA Displacement Assays with hFABP1.
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism"

Article Title: Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism

Journal: bioRxiv

doi: 10.1101/2024.01.17.576032

Arachidonic Acid as a Model Ligand for DAUDA Displacement Assays with hFABP1.
Figure Legend Snippet: Arachidonic Acid as a Model Ligand for DAUDA Displacement Assays with hFABP1.

Techniques Used: Fluorescence

DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine Kd values summarized in Table 1.
Figure Legend Snippet: DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine Kd values summarized in Table 1.

Techniques Used: Fluorescence, Titration, Binding Assay

Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. (A and B) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. (C and D) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F) Molecular stick models showing the distinct positions of (R)- (E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.
Figure Legend Snippet: Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. (A and B) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. (C and D) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F) Molecular stick models showing the distinct positions of (R)- (E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.

Techniques Used: Binding Assay


Structured Review

Cayman Chemical flurbiprofen fp
Flurbiprofen Fp, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen fp/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen fp - by Bioz Stars, 2024-06
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Structured Review

Cayman Chemical flurbiprofen
( a ) Drug release percentages of ketorolac and <t>flurbiprofen</t> for the 3-day loading time; ( b ) drug release concentrations for ketorolac and flurbiprofen for the 3-day loading time; ( c ) comparison of ketorolac release percentages for the 3-day and 10-day loading times; ( d ) comparison of flurbiprofen release percentages for the 3-day and 10-day loading times.
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "Parametric Drug Release Optimization of Anti-Inflammatory Drugs by Gold Nanoparticles for Topically Applied Ocular Therapy"

Article Title: Parametric Drug Release Optimization of Anti-Inflammatory Drugs by Gold Nanoparticles for Topically Applied Ocular Therapy

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms232416191

( a ) Drug release percentages of ketorolac and flurbiprofen for the 3-day loading time; ( b ) drug release concentrations for ketorolac and flurbiprofen for the 3-day loading time; ( c ) comparison of ketorolac release percentages for the 3-day and 10-day loading times; ( d ) comparison of flurbiprofen release percentages for the 3-day and 10-day loading times.
Figure Legend Snippet: ( a ) Drug release percentages of ketorolac and flurbiprofen for the 3-day loading time; ( b ) drug release concentrations for ketorolac and flurbiprofen for the 3-day loading time; ( c ) comparison of ketorolac release percentages for the 3-day and 10-day loading times; ( d ) comparison of flurbiprofen release percentages for the 3-day and 10-day loading times.

Techniques Used:

( a ) Drug release concentrations for ketorolac with and without AuNPs; ( b ) the associated ketorolac release percentages; ( c ) drug release concentrations for flurbiprofen with and without AuNPs; ( d ) the associated flurbiprofen released percentages.
Figure Legend Snippet: ( a ) Drug release concentrations for ketorolac with and without AuNPs; ( b ) the associated ketorolac release percentages; ( c ) drug release concentrations for flurbiprofen with and without AuNPs; ( d ) the associated flurbiprofen released percentages.

Techniques Used:


Structured Review

Cayman Chemical flurbiprofen
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 92 stars, based on 1 article reviews
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92/100 stars

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Structured Review

Cayman Chemical rac flurbiprofen
Rac Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rac flurbiprofen/product/Cayman Chemical
Average 92 stars, based on 1 article reviews
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Structured Review

Cayman Chemical flurbiprofen
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen - by Bioz Stars, 2024-06
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Structured Review

Cayman Chemical flurbiprofen
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
flurbiprofen - by Bioz Stars, 2024-06
86/100 stars

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Structured Review

Cayman Chemical flurbiprofen
Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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    Cayman Chemical flurbiprofen
    DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and <t>S-flurbiprofen,</t> gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .
    Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flurbiprofen/product/Cayman Chemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flurbiprofen - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cayman Chemical flurbiprofen fp
    DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and <t>S-flurbiprofen,</t> gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .
    Flurbiprofen Fp, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flurbiprofen fp/product/Cayman Chemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flurbiprofen fp - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    92
    Cayman Chemical rac flurbiprofen
    DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and <t>S-flurbiprofen,</t> gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .
    Rac Flurbiprofen, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rac flurbiprofen/product/Cayman Chemical
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rac flurbiprofen - by Bioz Stars, 2024-06
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    Image Search Results


    DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .

    Journal: bioRxiv

    Article Title: Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism

    doi: 10.1101/2024.01.17.576032

    Figure Lengend Snippet: DAUDA displacement from FABP1 by drug ligands. The fluorescence spectra for DAUDA displacement titrations with diazepam, diclofenac, R- and S-flurbiprofen, gemfibrozil, ibuprofen, pioglitazone, sulfaphenazole and tolbutamide as ligands are shown across the rows. In each titration spectra, the top dark blue spectrum represents DAUDA (0.5 µM) prebound with FABP1 (0.3 µM) in the absence of ligand and each subsequent colored spectrum represents increasing concentrations of ligand. The shaded area is the spectrum of 0.5 µM unbound DAUDA in the absence of FABP1 and ligand. Corresponding DAUDA displacement curves are shown below each spectra. A ternary complex binding model was fit to replicate experiments done on separate days (dark blue, green, gold and pink circles) to determine K d values summarized in .

    Article Snippet: Kanamycin, Trizma base (Tris), sodium chloride, sodium phosphate, potassium phosphate, protease inhibitor tablets, benzonase, thrombin, Coomassie Brilliant Blue R, 11-(Dansylamino)undecanoic acid (DAUDA), arachidonic acid, diazepam, diclofenac, fluoxetine, racemic flurbiprofen, gemfibrozil, ibuprofen, sulfaphenazole and tolbutamide were purchased from Millipore-Sigma (St. Louis, MO). (R)- and (S)-flurbiprofen were purchased from Cayman Chemical (Ann Arbor, MI).

    Techniques: Fluorescence, Titration, Binding Assay

    Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. ( A and B ) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. ( C and D ) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F ) Molecular stick models showing the distinct positions of (R)-(E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.

    Journal: bioRxiv

    Article Title: Drugs Form Ternary Complexes with Human Liver Fatty Acid Binding Protein (FABP1) and FABP1 Binding Alters Drug Metabolism

    doi: 10.1101/2024.01.17.576032

    Figure Lengend Snippet: Docking of (R)- and (S)-flurbiprofen to hFABP1 with DAUDA. ( A and B ) Potential binding orientations of singly bound (R)-flurbiprofen (A, blue) and (S)-flurbiprofen (B, orange) in the absence of DAUDA. ( C and D ) Binding orientations of (R)- and (S)-flurbiprofen are shown in the presence of DAUDA (gray) in the hFABP1 binding cavity. (E and F ) Molecular stick models showing the distinct positions of (R)-(E) and (S)-flurbiprofen (F) at the portal domain of hFABP1 in the presence of DAUDA in the binding cavity. Predicted hydrogen bonding for the carboxyl group of (S)-flurbiprofen is shown in green.

    Article Snippet: Kanamycin, Trizma base (Tris), sodium chloride, sodium phosphate, potassium phosphate, protease inhibitor tablets, benzonase, thrombin, Coomassie Brilliant Blue R, 11-(Dansylamino)undecanoic acid (DAUDA), arachidonic acid, diazepam, diclofenac, fluoxetine, racemic flurbiprofen, gemfibrozil, ibuprofen, sulfaphenazole and tolbutamide were purchased from Millipore-Sigma (St. Louis, MO). (R)- and (S)-flurbiprofen were purchased from Cayman Chemical (Ann Arbor, MI).

    Techniques: Binding Assay