fluospheres carboxylate modified microspheres  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher fluospheres carboxylate modified microspheres
    Effect of LECT2 on phagocytosis of mouse MΦ. (A) Uptake of E. coli –FITC or <t>FluoSpheres</t> with LECT2 treatment. (B) Flow cytometry histogram represents the E. coli –FITC phagocytosis by MΦ. (C) Uptake of E. coli– FITC by resting MΦ treated with LECT2 supernatant. *, P
    Fluospheres Carboxylate Modified Microspheres, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluospheres carboxylate modified microspheres/product/Thermo Fisher
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    fluospheres carboxylate modified microspheres - by Bioz Stars, 2022-10
    99/100 stars

    Images

    1) Product Images from "LECT2 protects mice against bacterial sepsis by activating macrophages via the CD209a receptor"

    Article Title: LECT2 protects mice against bacterial sepsis by activating macrophages via the CD209a receptor

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20121466

    Effect of LECT2 on phagocytosis of mouse MΦ. (A) Uptake of E. coli –FITC or FluoSpheres with LECT2 treatment. (B) Flow cytometry histogram represents the E. coli –FITC phagocytosis by MΦ. (C) Uptake of E. coli– FITC by resting MΦ treated with LECT2 supernatant. *, P
    Figure Legend Snippet: Effect of LECT2 on phagocytosis of mouse MΦ. (A) Uptake of E. coli –FITC or FluoSpheres with LECT2 treatment. (B) Flow cytometry histogram represents the E. coli –FITC phagocytosis by MΦ. (C) Uptake of E. coli– FITC by resting MΦ treated with LECT2 supernatant. *, P

    Techniques Used: Flow Cytometry, Cytometry

    2) Product Images from "Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms"

    Article Title: Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.033654

    Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.
    Figure Legend Snippet: Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.

    Techniques Used: Mutagenesis, Permeability, Injection, Transmission Electron Microscopy

    3) Product Images from "Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles"

    Article Title: Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S170832

    CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.
    Figure Legend Snippet: CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.

    Techniques Used: Confocal Laser Scanning Microscopy

    4) Product Images from "Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles"

    Article Title: Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S170832

    CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.
    Figure Legend Snippet: CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.

    Techniques Used: Confocal Laser Scanning Microscopy

    5) Product Images from "Royal jelly enhances antigen‐specific mucosal IgA response"

    Article Title: Royal jelly enhances antigen‐specific mucosal IgA response

    Journal: Food Science & Nutrition

    doi: 10.1002/fsn3.29

    Both RJ and protease‐treated royal jelly ( pRJ ) promote antigen transcytosis across M‐like cells. (a) Outline of transcytosis assay in the in vitro M cell model. (b) To investigate the effect of RJ (B) or pRJ (c) on transcytosis activity in in vitro M cell model, the monolayers including M‐like cells induced to differentiate by Raji B cells, RJ, or pRJ as well as Caco‐2 control monolayers treated with DMSO as a control experiment were incubated with FluoSpheres ® carboxylate‐modified microspheres. The significance of difference (nonrepeated measures ANOVA) is indicated as follows: **, P
    Figure Legend Snippet: Both RJ and protease‐treated royal jelly ( pRJ ) promote antigen transcytosis across M‐like cells. (a) Outline of transcytosis assay in the in vitro M cell model. (b) To investigate the effect of RJ (B) or pRJ (c) on transcytosis activity in in vitro M cell model, the monolayers including M‐like cells induced to differentiate by Raji B cells, RJ, or pRJ as well as Caco‐2 control monolayers treated with DMSO as a control experiment were incubated with FluoSpheres ® carboxylate‐modified microspheres. The significance of difference (nonrepeated measures ANOVA) is indicated as follows: **, P

    Techniques Used: In Vitro, Activity Assay, Incubation, Modification

    6) Product Images from "Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae"

    Article Title: Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae

    Journal: Veterinary Research

    doi: 10.1186/s13567-017-0489-3

    BoHV-4 replication in bovine genital mucosa explants at 72 h after direct injection in the lamina propria. Red represents the FluoSpheres and green indicates the BoHV-4 infected cells.
    Figure Legend Snippet: BoHV-4 replication in bovine genital mucosa explants at 72 h after direct injection in the lamina propria. Red represents the FluoSpheres and green indicates the BoHV-4 infected cells.

    Techniques Used: Injection, Infection

    7) Product Images from "Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles"

    Article Title: Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S170832

    CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.
    Figure Legend Snippet: CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.

    Techniques Used: Confocal Laser Scanning Microscopy

    8) Product Images from "Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model"

    Article Title: Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087478

    Acute HFD increases c-Fos in orexin neurons of the LHA projecting to the VTA. Upper sets of panels depict low magnification photomicrographs of the LHA of mice with stereotaxic injections of FluoSpheres in the VTA and allowed to eat HFD. Right column of images shows merge of orexin-IR (green fluorescent staining), FluoSpheres (red fluorescent), and c-Fos (black/purple staining) signals. Bottom set of panels show high magnification photomicrographs of the area marked in low magnification images. Numbered arrows point to labeled cells as follows: 1-triple-labeled cell; 2- double c-Fos/orexin-IR cell, negative for FluoSpheres; 3-double c-Fos/FluoSpheres cell, negative for orexin. Scale bars: 50 µm (Upper panels), 20 µm (Bottom panels).
    Figure Legend Snippet: Acute HFD increases c-Fos in orexin neurons of the LHA projecting to the VTA. Upper sets of panels depict low magnification photomicrographs of the LHA of mice with stereotaxic injections of FluoSpheres in the VTA and allowed to eat HFD. Right column of images shows merge of orexin-IR (green fluorescent staining), FluoSpheres (red fluorescent), and c-Fos (black/purple staining) signals. Bottom set of panels show high magnification photomicrographs of the area marked in low magnification images. Numbered arrows point to labeled cells as follows: 1-triple-labeled cell; 2- double c-Fos/orexin-IR cell, negative for FluoSpheres; 3-double c-Fos/FluoSpheres cell, negative for orexin. Scale bars: 50 µm (Upper panels), 20 µm (Bottom panels).

    Techniques Used: Mouse Assay, Staining, Labeling

    9) Product Images from "Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms"

    Article Title: Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.033654

    Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.
    Figure Legend Snippet: Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.

    Techniques Used: Mutagenesis, Permeability, Injection, Transmission Electron Microscopy

    10) Product Images from "Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms"

    Article Title: Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.033654

    Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.
    Figure Legend Snippet: Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.

    Techniques Used: Mutagenesis, Permeability, Injection, Transmission Electron Microscopy

    11) Product Images from "Human Flt3L Generates Dendritic Cells from Canine Peripheral Blood Precursors: Implications for a Dog Glioma Clinical Trial"

    Article Title: Human Flt3L Generates Dendritic Cells from Canine Peripheral Blood Precursors: Implications for a Dog Glioma Clinical Trial

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0011074

    DC phagocytic activity. Peripheral blood cultures were collected after 7 days in culture with recombinant canine IL-4 and GM-CSF or Ad.hFlt3L conditioned medium and incubated for additional 24 h without added cytokines in the absence (mock) or presence of CpG 2216 or LPS. Activated dog DCs were mixed with 1.0 µm yellow-green fluorescent FluoSpheres for 4 hrs at 4°C or 37°C. A , Confocal microphotographs show CD11c + and MHCII + cells (red) containing yellow-green beads in their cytoplasm. Nuclei were stained with DAPI (blue). Scale bars: 10 µm. B , Scatter plot shows the quantification of CD11c + DCs containing yellow-green fluorescent FluoSpheres in their cytoplasm. *p
    Figure Legend Snippet: DC phagocytic activity. Peripheral blood cultures were collected after 7 days in culture with recombinant canine IL-4 and GM-CSF or Ad.hFlt3L conditioned medium and incubated for additional 24 h without added cytokines in the absence (mock) or presence of CpG 2216 or LPS. Activated dog DCs were mixed with 1.0 µm yellow-green fluorescent FluoSpheres for 4 hrs at 4°C or 37°C. A , Confocal microphotographs show CD11c + and MHCII + cells (red) containing yellow-green beads in their cytoplasm. Nuclei were stained with DAPI (blue). Scale bars: 10 µm. B , Scatter plot shows the quantification of CD11c + DCs containing yellow-green fluorescent FluoSpheres in their cytoplasm. *p

    Techniques Used: Activity Assay, Recombinant, Incubation, Staining

    12) Product Images from "Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms"

    Article Title: Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.033654

    Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.
    Figure Legend Snippet: Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.

    Techniques Used: Mutagenesis, Permeability, Injection, Transmission Electron Microscopy

    13) Product Images from "Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles"

    Article Title: Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S170832

    CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.
    Figure Legend Snippet: CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.

    Techniques Used: Confocal Laser Scanning Microscopy

    14) Product Images from "Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles"

    Article Title: Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S170832

    CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.
    Figure Legend Snippet: CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.

    Techniques Used: Confocal Laser Scanning Microscopy

    15) Product Images from "Dual oxidase 1 limits the IFNγ-associated antitumor effect of macrophages"

    Article Title: Dual oxidase 1 limits the IFNγ-associated antitumor effect of macrophages

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1136/jitc-2020-000622

    DUOX1 controls the phagocytotic function of macrophages both in vitro and in vivo . (A) Macrophage precursors from WT or Duox1 −/− bone marrow were cultured in the presence of either M-CSF or GM-CSF. After 6 days, fluorescent beads (FluoSpheres) were added to the bone marrow–derived macrophages (BMDMs) for 30 min. (B and C, left panels) M-CSF (B) and GM-CSF (C) cultured BMDMs were analyzed by flow cytometry for their ability to take up the FluoSpheres, which is represented as the fold increases in mean fluorescence intensity (∆MFI=MFI of the specific fluorescence of beads−MFI of control (non-treated BMDMs)). The right panels show histograms of FluoSphere − (Ctrl) and FluoSphere + BMDMs. Data were obtained from two independent experiments and are represented as the mean±SEM. n=7–8, *p
    Figure Legend Snippet: DUOX1 controls the phagocytotic function of macrophages both in vitro and in vivo . (A) Macrophage precursors from WT or Duox1 −/− bone marrow were cultured in the presence of either M-CSF or GM-CSF. After 6 days, fluorescent beads (FluoSpheres) were added to the bone marrow–derived macrophages (BMDMs) for 30 min. (B and C, left panels) M-CSF (B) and GM-CSF (C) cultured BMDMs were analyzed by flow cytometry for their ability to take up the FluoSpheres, which is represented as the fold increases in mean fluorescence intensity (∆MFI=MFI of the specific fluorescence of beads−MFI of control (non-treated BMDMs)). The right panels show histograms of FluoSphere − (Ctrl) and FluoSphere + BMDMs. Data were obtained from two independent experiments and are represented as the mean±SEM. n=7–8, *p

    Techniques Used: In Vitro, In Vivo, Cell Culture, Derivative Assay, Flow Cytometry, Fluorescence

    16) Product Images from "Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles"

    Article Title: Comparison of normal versus imiquimod-induced psoriatic skin in mice for penetration of drugs and nanoparticles

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S170832

    CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.
    Figure Legend Snippet: CLSM visualization of vertical sections from normal and imiquimod-induced psoriatic skins, which were treated with 40-, 100-, and 500-nm-sized FluoSpheres ® for 24 hours at 32°C. Notes: Two views were given to present the wide structure of skin penetration ( A , 200×), as well as the enlarged field of the distribution around hair follicles ( B , 1,260×). For picture A , bar =100 µm, while for picture B , bar =25 µm. The emission wavelength was between 590 and 656 nm for 40 and 100-nm-sized FluoSphere ® when the excitation wavelength was set as 552 nm, while the emission wavelength was between 495 and 545 nm for 500-nm-sized FluoSphere ® when the excitation wavelength was set as 488 nm. The white arrows indicate the hair follicles. Abbreviations: CLSM, confocal laser scanning microscopy; IMQ, imiquimod.

    Techniques Used: Confocal Laser Scanning Microscopy

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher fluospheres carboxylate modified microspheres
    Effect of LECT2 on phagocytosis of mouse MΦ. (A) Uptake of E. coli –FITC or <t>FluoSpheres</t> with LECT2 treatment. (B) Flow cytometry histogram represents the E. coli –FITC phagocytosis by MΦ. (C) Uptake of E. coli– FITC by resting MΦ treated with LECT2 supernatant. *, P
    Fluospheres Carboxylate Modified Microspheres, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluospheres carboxylate modified microspheres/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluospheres carboxylate modified microspheres - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    96
    Thermo Fisher carboxylate modified fluospheres beads
    Height and shape of colonies . Z stacks, with planes separated by 10 μm, of colonies formed by B. subtilis F29-3(pHag-gfp), B. subtilis FW463(pHag-gfp) and B. subtilis FK955(pHag-gfp) were acquired using a confocal laser-scanning microscope. Bacteria were cultured on 8.5-ml LB-0.4 agar plates containing red carboxylate-modified <t>FluoSpheres</t> beads (Invitrogen). The last plane with red fluorescence outside the green colony area indicates the agar surface.
    Carboxylate Modified Fluospheres Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carboxylate modified fluospheres beads/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    carboxylate modified fluospheres beads - by Bioz Stars, 2022-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Effect of LECT2 on phagocytosis of mouse MΦ. (A) Uptake of E. coli –FITC or FluoSpheres with LECT2 treatment. (B) Flow cytometry histogram represents the E. coli –FITC phagocytosis by MΦ. (C) Uptake of E. coli– FITC by resting MΦ treated with LECT2 supernatant. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: LECT2 protects mice against bacterial sepsis by activating macrophages via the CD209a receptor

    doi: 10.1084/jem.20121466

    Figure Lengend Snippet: Effect of LECT2 on phagocytosis of mouse MΦ. (A) Uptake of E. coli –FITC or FluoSpheres with LECT2 treatment. (B) Flow cytometry histogram represents the E. coli –FITC phagocytosis by MΦ. (C) Uptake of E. coli– FITC by resting MΦ treated with LECT2 supernatant. *, P

    Article Snippet: FluoSpheres (F8827) were purchased from Molecular Probes.

    Techniques: Flow Cytometry, Cytometry

    Height and shape of colonies . Z stacks, with planes separated by 10 μm, of colonies formed by B. subtilis F29-3(pHag-gfp), B. subtilis FW463(pHag-gfp) and B. subtilis FK955(pHag-gfp) were acquired using a confocal laser-scanning microscope. Bacteria were cultured on 8.5-ml LB-0.4 agar plates containing red carboxylate-modified FluoSpheres beads (Invitrogen). The last plane with red fluorescence outside the green colony area indicates the agar surface.

    Journal: Frontiers in Microbiology

    Article Title: Water surface tension modulates the swarming mechanics of Bacillus subtilis

    doi: 10.3389/fmicb.2015.01017

    Figure Lengend Snippet: Height and shape of colonies . Z stacks, with planes separated by 10 μm, of colonies formed by B. subtilis F29-3(pHag-gfp), B. subtilis FW463(pHag-gfp) and B. subtilis FK955(pHag-gfp) were acquired using a confocal laser-scanning microscope. Bacteria were cultured on 8.5-ml LB-0.4 agar plates containing red carboxylate-modified FluoSpheres beads (Invitrogen). The last plane with red fluorescence outside the green colony area indicates the agar surface.

    Article Snippet: To measure the height of colonies formed by GFP-expressing B. subtilis F29-3, bacteria were cultured on an LB-0.4 agar plate that contained 1/100th of its volume of 0.5 μm carboxylate-modified FluoSpheres beads (Invitrogen, Ex580/Em605).

    Techniques: Laser-Scanning Microscopy, Cell Culture, Modification, Fluorescence

    The blood–brain barrier is permeable in superoxide dismutase-1 (SOD1)G93A mice. A: Section though spinal cord of an ALS mouse (age 100 days) perfused with 0.2 μm Fluospheres (F8810, Invitrogen) shown in red (n=3). The actin cytoskeleton is green and blue defines the nuclei (bar=250 μm). Beads are present throughout the soma of the spinal cord (Panel B). However, in TBI/transplanted mouse at 30 days post transplantation (Panel C) no beads are present in the spinal cord.

    Journal: In Vivo

    Article Title: Amelioration of Amyotrophic Lateral Sclerosis in SOD1G93A Mice by M2 Microglia from Transplanted Marrow

    doi: 10.21873/invivo.11526

    Figure Lengend Snippet: The blood–brain barrier is permeable in superoxide dismutase-1 (SOD1)G93A mice. A: Section though spinal cord of an ALS mouse (age 100 days) perfused with 0.2 μm Fluospheres (F8810, Invitrogen) shown in red (n=3). The actin cytoskeleton is green and blue defines the nuclei (bar=250 μm). Beads are present throughout the soma of the spinal cord (Panel B). However, in TBI/transplanted mouse at 30 days post transplantation (Panel C) no beads are present in the spinal cord.

    Article Snippet: Blood–brain barrier permeability was analyzed by intravenous perfusion with 0.2 μm Fluorospheres (F8810; Invitrogen) on day 125 in control SOD1G93A mice, SOD1G93A mice transplanted on day 90 with GFP+ C57BL6 bone marrow and C57BL/6 mice.

    Techniques: Mouse Assay, Transplantation Assay

    Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.

    Journal: Disease Models & Mechanisms

    Article Title: Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms

    doi: 10.1242/dmm.033654

    Figure Lengend Snippet: Homozygous col22a1 −/− mutant embryos show dilated and dysmorphic cranial vessels and have increased vascular permeability. (A-D) Vascular patterning is not affected in col22a1 mutants at 3 dpf and 4 dpf. Confocal maximal-intensity projections of live kdrl:GFP embryos, dorsal view, anterior is to the top. (E-J) Dilated and dysmorphic vessels are observed in col22a1 mutants. Note the dilated and fused vessels at the vascular branch point in the periocular region (arrows) and the dilated and dysmorphic palatocerebral vein (PLV, arrowheads). Higher magnification (40×) images of the PLV are shown in I and J. Note that a portion of the PLV forms a plexus (arrow, J), which is not observed in WT embryos. Confocal maximal-intensity projections of selected slices of live kdrl:GFP embryos are shown, dorsal view, anterior is to the top. Cross-sections in the PLV, lateral dorsal aorta (LDA, E) and at the periocular branch point (asterisk, G) label the vessels selected for diameter measurement in Q. Different Z -stack projections of the same embryos are shown in E and G, and in F and H; I and J show different embryos. (K-P) Microangiography analysis of vascular permeability. FluoSpheres polystyrene microspheres (red, K,L) or low Mw (10 kDa) TRITC-dextran (red, M-P) were injected into the circulatory system of kdrl:GFP -positive WT and col22a1 −/− embryos. Dorsal views of merged images (red, dextran/microspheres; green, kdrl:GFP ) show rhodamine dye leakage at the brain ventricle and choroid plexus (dashed lines, N,P) and microsphere accumulation outside the vasculature (dashed lines, K,L) in col22a1 −/− embryos at 3 dpf and 4 dpf. Maximal-intensity projections of confocal Z -stacks are shown. (Q) Vessel diameters of the LDA (E), PLV and the periocular branching point (asterisk, G) of heat-stressed col22a1 mutants and wild-type (wt) embryos at 3 dpf. The measurements were made at three randomly selected points for each vessel in 12 mutant and wt embryos obtained in two independent experiments. LDA and periocular vessels were measured at both left and right sides where possible. The total number of measured points n =57 for LDA (s.d.=1.9 and 2.5 for wt and mutant embryos, respectively), n =54 and 57 for the periocular vessel (s.d.=4.3 and 6.5 for wt and mutant embryos, respectively), and n =36 for the PLV (s.d.=3.2 and 5.9 for wt and mutant embryos, respectively). P -values were calculated using Student's t -test between wt and col22a1 −/− embryos for all measured points in all embryos. (R,S) TEM analysis of cranial vascular endothelium. Note the highly dysmorphic vascular endothelium (arrows, S) in col22a1 mutants compared with normal vascular endothelium in WT embryos (arrowheads, R). RBC, red blood cells.

    Article Snippet: Heat-stressed live 72 hpf or 96 hpf embryos were injected into the perivitelline space with 10 kDa TRITC-dextran (Molecular Probes, Life Technologies, D1817), or with FluoSpheres carboxylate-modified microspheres 0.02 µm, red fluorescent (Molecular Probes, Invitrogen, F8786), and were embedded in low-melting-point agarose and immediately imaged using a Nikon A1R LUN-V inverted microscope with 20×/0.75 Multi immersion objective at the CCHMC Confocal Imaging Core facility.

    Techniques: Mutagenesis, Permeability, Injection, Transmission Electron Microscopy